Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells

Detalhes bibliográficos
Autor(a) principal: Nunes, Viviane A. [UNIFESP]
Data de Publicação: 2003
Outros Autores: Gozzo, Andrezza Justino [UNIFESP], Juliano, Maria Aparecida [UNIFESP], Cerqueira César, M., Sampaio, Misako Uemura [UNIFESP], Sampaio, Claudio a.m. [UNIFESP], Araujo, Mariana da Silva [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0100-879X2003000800010
http://repositorio.unifesp.br/handle/11600/1809
Resumo: Apoptosis and necrosis are two distinct forms of cell death that can occur in response to different agents and stress conditions. In order to verify if the oxidative stress induced by dietary selenium and vitamin E deficiencies can lead muscle cells to apoptosis, one-day-old chicks were reared using diets differing in their vitamin E (0 or 10 IU/kg) and selenium (0 or 0.15 ppm) supplementation. Chick skeletal muscle tissue was obtained from 28-day-old animals and used to verify apoptosis occurrence based on caspase activity detection and DNA fragmentation. Antioxidant deficiency significantly increased caspase-like activity assessed by the hydrolysis of fluorogenic peptide substrates (Abz-peptidyl-EDDnp) at lambdaexc = 320 nm and lambdaem = 420 nm. Proteolytic activation was not accompanied by typical internucleosomal DNA fragmentation detected by field inversion gel electrophoresis. Although the general caspase inhibitor N-benzyloxycarbonyl-Val-Ala-Asp(O-Me) fluoromethyl ketone (Z-VAD-fmk) (0 to 80 muM) did not block caspase-like activity when preincubated for 30 min with muscle homogenates, the hydrolyzed substrates presented the same cleavage profile in HPLC (at the aspartic acid residue) when incubated with the purified recombinant enzyme caspase-3. These data indicate that oxidative stress causes caspase-like activation in muscle cells and suggest that cell death associated with exudative diathesis (dietary deficiency of selenium and vitamin E) can follow the apoptotic pathway.
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spelling Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cellsOxidative stressCaspase activationApoptosisDietary selenium deficiencyDietary vitamin E deficiencyInternucleosomal DNA fragmentationCaspase substrateApoptosis and necrosis are two distinct forms of cell death that can occur in response to different agents and stress conditions. In order to verify if the oxidative stress induced by dietary selenium and vitamin E deficiencies can lead muscle cells to apoptosis, one-day-old chicks were reared using diets differing in their vitamin E (0 or 10 IU/kg) and selenium (0 or 0.15 ppm) supplementation. Chick skeletal muscle tissue was obtained from 28-day-old animals and used to verify apoptosis occurrence based on caspase activity detection and DNA fragmentation. Antioxidant deficiency significantly increased caspase-like activity assessed by the hydrolysis of fluorogenic peptide substrates (Abz-peptidyl-EDDnp) at lambdaexc = 320 nm and lambdaem = 420 nm. Proteolytic activation was not accompanied by typical internucleosomal DNA fragmentation detected by field inversion gel electrophoresis. Although the general caspase inhibitor N-benzyloxycarbonyl-Val-Ala-Asp(O-Me) fluoromethyl ketone (Z-VAD-fmk) (0 to 80 muM) did not block caspase-like activity when preincubated for 30 min with muscle homogenates, the hydrolyzed substrates presented the same cleavage profile in HPLC (at the aspartic acid residue) when incubated with the purified recombinant enzyme caspase-3. These data indicate that oxidative stress causes caspase-like activation in muscle cells and suggest that cell death associated with exudative diathesis (dietary deficiency of selenium and vitamin E) can follow the apoptotic pathway.Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de BioquímicaUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de BiofísicaUniversidade de São Paulo Faculdade de Zootecnia e Engenharia de Alimentos Departamento de CiênciasUNIFESP, EPM, Depto. de BioquímicaUNIFESP, EPM, Depto. de BiofísicaSciELOAssociação Brasileira de Divulgação CientíficaUniversidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Nunes, Viviane A. [UNIFESP]Gozzo, Andrezza Justino [UNIFESP]Juliano, Maria Aparecida [UNIFESP]Cerqueira César, M.Sampaio, Misako Uemura [UNIFESP]Sampaio, Claudio a.m. [UNIFESP]Araujo, Mariana da Silva [UNIFESP]2015-06-14T13:30:06Z2015-06-14T13:30:06Z2003-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1047-1053application/pdfhttp://dx.doi.org/10.1590/S0100-879X2003000800010Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 36, n. 8, p. 1047-1053, 2003.10.1590/S0100-879X2003000800010S0100-879X2003000800010.pdf0100-879XS0100-879X2003000800010http://repositorio.unifesp.br/handle/11600/1809WOS:000185012900011engBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-29T17:31:59Zoai:repositorio.unifesp.br/:11600/1809Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-29T17:31:59Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells
title Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells
spellingShingle Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells
Nunes, Viviane A. [UNIFESP]
Oxidative stress
Caspase activation
Apoptosis
Dietary selenium deficiency
Dietary vitamin E deficiency
Internucleosomal DNA fragmentation
Caspase substrate
title_short Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells
title_full Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells
title_fullStr Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells
title_full_unstemmed Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells
title_sort Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells
author Nunes, Viviane A. [UNIFESP]
author_facet Nunes, Viviane A. [UNIFESP]
Gozzo, Andrezza Justino [UNIFESP]
Juliano, Maria Aparecida [UNIFESP]
Cerqueira César, M.
Sampaio, Misako Uemura [UNIFESP]
Sampaio, Claudio a.m. [UNIFESP]
Araujo, Mariana da Silva [UNIFESP]
author_role author
author2 Gozzo, Andrezza Justino [UNIFESP]
Juliano, Maria Aparecida [UNIFESP]
Cerqueira César, M.
Sampaio, Misako Uemura [UNIFESP]
Sampaio, Claudio a.m. [UNIFESP]
Araujo, Mariana da Silva [UNIFESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Nunes, Viviane A. [UNIFESP]
Gozzo, Andrezza Justino [UNIFESP]
Juliano, Maria Aparecida [UNIFESP]
Cerqueira César, M.
Sampaio, Misako Uemura [UNIFESP]
Sampaio, Claudio a.m. [UNIFESP]
Araujo, Mariana da Silva [UNIFESP]
dc.subject.por.fl_str_mv Oxidative stress
Caspase activation
Apoptosis
Dietary selenium deficiency
Dietary vitamin E deficiency
Internucleosomal DNA fragmentation
Caspase substrate
topic Oxidative stress
Caspase activation
Apoptosis
Dietary selenium deficiency
Dietary vitamin E deficiency
Internucleosomal DNA fragmentation
Caspase substrate
description Apoptosis and necrosis are two distinct forms of cell death that can occur in response to different agents and stress conditions. In order to verify if the oxidative stress induced by dietary selenium and vitamin E deficiencies can lead muscle cells to apoptosis, one-day-old chicks were reared using diets differing in their vitamin E (0 or 10 IU/kg) and selenium (0 or 0.15 ppm) supplementation. Chick skeletal muscle tissue was obtained from 28-day-old animals and used to verify apoptosis occurrence based on caspase activity detection and DNA fragmentation. Antioxidant deficiency significantly increased caspase-like activity assessed by the hydrolysis of fluorogenic peptide substrates (Abz-peptidyl-EDDnp) at lambdaexc = 320 nm and lambdaem = 420 nm. Proteolytic activation was not accompanied by typical internucleosomal DNA fragmentation detected by field inversion gel electrophoresis. Although the general caspase inhibitor N-benzyloxycarbonyl-Val-Ala-Asp(O-Me) fluoromethyl ketone (Z-VAD-fmk) (0 to 80 muM) did not block caspase-like activity when preincubated for 30 min with muscle homogenates, the hydrolyzed substrates presented the same cleavage profile in HPLC (at the aspartic acid residue) when incubated with the purified recombinant enzyme caspase-3. These data indicate that oxidative stress causes caspase-like activation in muscle cells and suggest that cell death associated with exudative diathesis (dietary deficiency of selenium and vitamin E) can follow the apoptotic pathway.
publishDate 2003
dc.date.none.fl_str_mv 2003-08-01
2015-06-14T13:30:06Z
2015-06-14T13:30:06Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-879X2003000800010
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 36, n. 8, p. 1047-1053, 2003.
10.1590/S0100-879X2003000800010
S0100-879X2003000800010.pdf
0100-879X
S0100-879X2003000800010
http://repositorio.unifesp.br/handle/11600/1809
WOS:000185012900011
url http://dx.doi.org/10.1590/S0100-879X2003000800010
http://repositorio.unifesp.br/handle/11600/1809
identifier_str_mv Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 36, n. 8, p. 1047-1053, 2003.
10.1590/S0100-879X2003000800010
S0100-879X2003000800010.pdf
0100-879X
S0100-879X2003000800010
WOS:000185012900011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Medical and Biological Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1047-1053
application/pdf
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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