Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1590/S0100-879X2003000800010 http://repositorio.unifesp.br/handle/11600/1809 |
Resumo: | Apoptosis and necrosis are two distinct forms of cell death that can occur in response to different agents and stress conditions. In order to verify if the oxidative stress induced by dietary selenium and vitamin E deficiencies can lead muscle cells to apoptosis, one-day-old chicks were reared using diets differing in their vitamin E (0 or 10 IU/kg) and selenium (0 or 0.15 ppm) supplementation. Chick skeletal muscle tissue was obtained from 28-day-old animals and used to verify apoptosis occurrence based on caspase activity detection and DNA fragmentation. Antioxidant deficiency significantly increased caspase-like activity assessed by the hydrolysis of fluorogenic peptide substrates (Abz-peptidyl-EDDnp) at lambdaexc = 320 nm and lambdaem = 420 nm. Proteolytic activation was not accompanied by typical internucleosomal DNA fragmentation detected by field inversion gel electrophoresis. Although the general caspase inhibitor N-benzyloxycarbonyl-Val-Ala-Asp(O-Me) fluoromethyl ketone (Z-VAD-fmk) (0 to 80 muM) did not block caspase-like activity when preincubated for 30 min with muscle homogenates, the hydrolyzed substrates presented the same cleavage profile in HPLC (at the aspartic acid residue) when incubated with the purified recombinant enzyme caspase-3. These data indicate that oxidative stress causes caspase-like activation in muscle cells and suggest that cell death associated with exudative diathesis (dietary deficiency of selenium and vitamin E) can follow the apoptotic pathway. |
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Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cellsOxidative stressCaspase activationApoptosisDietary selenium deficiencyDietary vitamin E deficiencyInternucleosomal DNA fragmentationCaspase substrateApoptosis and necrosis are two distinct forms of cell death that can occur in response to different agents and stress conditions. In order to verify if the oxidative stress induced by dietary selenium and vitamin E deficiencies can lead muscle cells to apoptosis, one-day-old chicks were reared using diets differing in their vitamin E (0 or 10 IU/kg) and selenium (0 or 0.15 ppm) supplementation. Chick skeletal muscle tissue was obtained from 28-day-old animals and used to verify apoptosis occurrence based on caspase activity detection and DNA fragmentation. Antioxidant deficiency significantly increased caspase-like activity assessed by the hydrolysis of fluorogenic peptide substrates (Abz-peptidyl-EDDnp) at lambdaexc = 320 nm and lambdaem = 420 nm. Proteolytic activation was not accompanied by typical internucleosomal DNA fragmentation detected by field inversion gel electrophoresis. Although the general caspase inhibitor N-benzyloxycarbonyl-Val-Ala-Asp(O-Me) fluoromethyl ketone (Z-VAD-fmk) (0 to 80 muM) did not block caspase-like activity when preincubated for 30 min with muscle homogenates, the hydrolyzed substrates presented the same cleavage profile in HPLC (at the aspartic acid residue) when incubated with the purified recombinant enzyme caspase-3. These data indicate that oxidative stress causes caspase-like activation in muscle cells and suggest that cell death associated with exudative diathesis (dietary deficiency of selenium and vitamin E) can follow the apoptotic pathway.Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de BioquímicaUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de BiofísicaUniversidade de São Paulo Faculdade de Zootecnia e Engenharia de Alimentos Departamento de CiênciasUNIFESP, EPM, Depto. de BioquímicaUNIFESP, EPM, Depto. de BiofísicaSciELOAssociação Brasileira de Divulgação CientíficaUniversidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Nunes, Viviane A. [UNIFESP]Gozzo, Andrezza Justino [UNIFESP]Juliano, Maria Aparecida [UNIFESP]Cerqueira César, M.Sampaio, Misako Uemura [UNIFESP]Sampaio, Claudio a.m. [UNIFESP]Araujo, Mariana da Silva [UNIFESP]2015-06-14T13:30:06Z2015-06-14T13:30:06Z2003-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1047-1053application/pdfhttp://dx.doi.org/10.1590/S0100-879X2003000800010Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 36, n. 8, p. 1047-1053, 2003.10.1590/S0100-879X2003000800010S0100-879X2003000800010.pdf0100-879XS0100-879X2003000800010http://repositorio.unifesp.br/handle/11600/1809WOS:000185012900011engBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-29T17:31:59Zoai:repositorio.unifesp.br/:11600/1809Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-29T17:31:59Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells |
title |
Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells |
spellingShingle |
Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells Nunes, Viviane A. [UNIFESP] Oxidative stress Caspase activation Apoptosis Dietary selenium deficiency Dietary vitamin E deficiency Internucleosomal DNA fragmentation Caspase substrate |
title_short |
Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells |
title_full |
Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells |
title_fullStr |
Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells |
title_full_unstemmed |
Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells |
title_sort |
Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells |
author |
Nunes, Viviane A. [UNIFESP] |
author_facet |
Nunes, Viviane A. [UNIFESP] Gozzo, Andrezza Justino [UNIFESP] Juliano, Maria Aparecida [UNIFESP] Cerqueira César, M. Sampaio, Misako Uemura [UNIFESP] Sampaio, Claudio a.m. [UNIFESP] Araujo, Mariana da Silva [UNIFESP] |
author_role |
author |
author2 |
Gozzo, Andrezza Justino [UNIFESP] Juliano, Maria Aparecida [UNIFESP] Cerqueira César, M. Sampaio, Misako Uemura [UNIFESP] Sampaio, Claudio a.m. [UNIFESP] Araujo, Mariana da Silva [UNIFESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Nunes, Viviane A. [UNIFESP] Gozzo, Andrezza Justino [UNIFESP] Juliano, Maria Aparecida [UNIFESP] Cerqueira César, M. Sampaio, Misako Uemura [UNIFESP] Sampaio, Claudio a.m. [UNIFESP] Araujo, Mariana da Silva [UNIFESP] |
dc.subject.por.fl_str_mv |
Oxidative stress Caspase activation Apoptosis Dietary selenium deficiency Dietary vitamin E deficiency Internucleosomal DNA fragmentation Caspase substrate |
topic |
Oxidative stress Caspase activation Apoptosis Dietary selenium deficiency Dietary vitamin E deficiency Internucleosomal DNA fragmentation Caspase substrate |
description |
Apoptosis and necrosis are two distinct forms of cell death that can occur in response to different agents and stress conditions. In order to verify if the oxidative stress induced by dietary selenium and vitamin E deficiencies can lead muscle cells to apoptosis, one-day-old chicks were reared using diets differing in their vitamin E (0 or 10 IU/kg) and selenium (0 or 0.15 ppm) supplementation. Chick skeletal muscle tissue was obtained from 28-day-old animals and used to verify apoptosis occurrence based on caspase activity detection and DNA fragmentation. Antioxidant deficiency significantly increased caspase-like activity assessed by the hydrolysis of fluorogenic peptide substrates (Abz-peptidyl-EDDnp) at lambdaexc = 320 nm and lambdaem = 420 nm. Proteolytic activation was not accompanied by typical internucleosomal DNA fragmentation detected by field inversion gel electrophoresis. Although the general caspase inhibitor N-benzyloxycarbonyl-Val-Ala-Asp(O-Me) fluoromethyl ketone (Z-VAD-fmk) (0 to 80 muM) did not block caspase-like activity when preincubated for 30 min with muscle homogenates, the hydrolyzed substrates presented the same cleavage profile in HPLC (at the aspartic acid residue) when incubated with the purified recombinant enzyme caspase-3. These data indicate that oxidative stress causes caspase-like activation in muscle cells and suggest that cell death associated with exudative diathesis (dietary deficiency of selenium and vitamin E) can follow the apoptotic pathway. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-08-01 2015-06-14T13:30:06Z 2015-06-14T13:30:06Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-879X2003000800010 Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 36, n. 8, p. 1047-1053, 2003. 10.1590/S0100-879X2003000800010 S0100-879X2003000800010.pdf 0100-879X S0100-879X2003000800010 http://repositorio.unifesp.br/handle/11600/1809 WOS:000185012900011 |
url |
http://dx.doi.org/10.1590/S0100-879X2003000800010 http://repositorio.unifesp.br/handle/11600/1809 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 36, n. 8, p. 1047-1053, 2003. 10.1590/S0100-879X2003000800010 S0100-879X2003000800010.pdf 0100-879X S0100-879X2003000800010 WOS:000185012900011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1047-1053 application/pdf |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
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1814268305570004992 |