Effects of 'casoparan', a peptide isolated from casein hydrolysates with mastoparan-like properties
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
dARK ID: | ark:/48912/001300000h9b6 |
Texto Completo: | http://dx.doi.org/10.1080/09629350400003068 http://repositorio.unifesp.br/handle/11600/27841 |
Resumo: | CASEIN, a protein found in milk of several species, is divided into different chains from 19 to 25 kappaDa. Casein is also considered as a source of amino acids and generating peptides with biological activities such as opiate, immunostimulating, antibacterial, peptidase inhibitors, among others. in this work, Sephadex G-10 chromatography followed by high-performance liquid chromatography isolation purified NZCase TT, an industrial culture media for tetanus toxin production. in the first step, four pools were isolated and tested in different assays: isolated smooth muscle assay ( guinea pig ileum, rat uterus), phagocytosis in vitro of opsonized sheep red blood cells, and hydrogen peroxide ( H2O2) release from mouse peritoneal macrophages. Pool III was the main active pool being able to potentiate bradykinin action in guinea pig ileum, stimulating phagocitic activity by resident macrophages and increasing H2O2 release from macrophages previously activated with bacille Calmette Guerin. Using mass spectra the primary structure of the main peptide from pool III was obtained-INKKI, which corresponds to beta-casein fragment 26-30. the immunostimulating action is probably related to a direct action in macrophage cells. |
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Effects of 'casoparan', a peptide isolated from casein hydrolysates with mastoparan-like propertiesmastoparancasein peptidesinflammationmacrophagespeptide isolationCASEIN, a protein found in milk of several species, is divided into different chains from 19 to 25 kappaDa. Casein is also considered as a source of amino acids and generating peptides with biological activities such as opiate, immunostimulating, antibacterial, peptidase inhibitors, among others. in this work, Sephadex G-10 chromatography followed by high-performance liquid chromatography isolation purified NZCase TT, an industrial culture media for tetanus toxin production. in the first step, four pools were isolated and tested in different assays: isolated smooth muscle assay ( guinea pig ileum, rat uterus), phagocytosis in vitro of opsonized sheep red blood cells, and hydrogen peroxide ( H2O2) release from mouse peritoneal macrophages. Pool III was the main active pool being able to potentiate bradykinin action in guinea pig ileum, stimulating phagocitic activity by resident macrophages and increasing H2O2 release from macrophages previously activated with bacille Calmette Guerin. Using mass spectra the primary structure of the main peptide from pool III was obtained-INKKI, which corresponds to beta-casein fragment 26-30. the immunostimulating action is probably related to a direct action in macrophage cells.Butantan Inst, Biochem & Biophys Lab, São Paulo, BrazilButantan Inst, Physiopathol Lab, São Paulo, BrazilUNIFESP, Dept Biophys, São Paulo, BrazilUNIFESP, Dept Biophys, São Paulo, BrazilWeb of ScienceCarfax PublishingButantan InstUniversidade Federal de São Paulo (UNIFESP)Lebrun, ICavallaro, VJuliano, Luiz [UNIFESP]Juliano, Maria Aparecida [UNIFESP]Silva, MCCDE2016-01-24T12:37:16Z2016-01-24T12:37:16Z2004-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion263-268http://dx.doi.org/10.1080/09629350400003068Mediators of Inflammation. Basingstoke: Carfax Publishing, v. 13, n. 4, p. 263-268, 2004.10.1080/096293504000030680962-9351http://repositorio.unifesp.br/handle/11600/27841WOS:000224975100005ark:/48912/001300000h9b6engMediators of Inflammationinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2021-09-29T11:23:43Zoai:repositorio.unifesp.br/:11600/27841Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T20:18:48.363825Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Effects of 'casoparan', a peptide isolated from casein hydrolysates with mastoparan-like properties |
title |
Effects of 'casoparan', a peptide isolated from casein hydrolysates with mastoparan-like properties |
spellingShingle |
Effects of 'casoparan', a peptide isolated from casein hydrolysates with mastoparan-like properties Lebrun, I mastoparan casein peptides inflammation macrophages peptide isolation |
title_short |
Effects of 'casoparan', a peptide isolated from casein hydrolysates with mastoparan-like properties |
title_full |
Effects of 'casoparan', a peptide isolated from casein hydrolysates with mastoparan-like properties |
title_fullStr |
Effects of 'casoparan', a peptide isolated from casein hydrolysates with mastoparan-like properties |
title_full_unstemmed |
Effects of 'casoparan', a peptide isolated from casein hydrolysates with mastoparan-like properties |
title_sort |
Effects of 'casoparan', a peptide isolated from casein hydrolysates with mastoparan-like properties |
author |
Lebrun, I |
author_facet |
Lebrun, I Cavallaro, V Juliano, Luiz [UNIFESP] Juliano, Maria Aparecida [UNIFESP] Silva, MCCDE |
author_role |
author |
author2 |
Cavallaro, V Juliano, Luiz [UNIFESP] Juliano, Maria Aparecida [UNIFESP] Silva, MCCDE |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Butantan Inst Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Lebrun, I Cavallaro, V Juliano, Luiz [UNIFESP] Juliano, Maria Aparecida [UNIFESP] Silva, MCCDE |
dc.subject.por.fl_str_mv |
mastoparan casein peptides inflammation macrophages peptide isolation |
topic |
mastoparan casein peptides inflammation macrophages peptide isolation |
description |
CASEIN, a protein found in milk of several species, is divided into different chains from 19 to 25 kappaDa. Casein is also considered as a source of amino acids and generating peptides with biological activities such as opiate, immunostimulating, antibacterial, peptidase inhibitors, among others. in this work, Sephadex G-10 chromatography followed by high-performance liquid chromatography isolation purified NZCase TT, an industrial culture media for tetanus toxin production. in the first step, four pools were isolated and tested in different assays: isolated smooth muscle assay ( guinea pig ileum, rat uterus), phagocytosis in vitro of opsonized sheep red blood cells, and hydrogen peroxide ( H2O2) release from mouse peritoneal macrophages. Pool III was the main active pool being able to potentiate bradykinin action in guinea pig ileum, stimulating phagocitic activity by resident macrophages and increasing H2O2 release from macrophages previously activated with bacille Calmette Guerin. Using mass spectra the primary structure of the main peptide from pool III was obtained-INKKI, which corresponds to beta-casein fragment 26-30. the immunostimulating action is probably related to a direct action in macrophage cells. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-08-01 2016-01-24T12:37:16Z 2016-01-24T12:37:16Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/09629350400003068 Mediators of Inflammation. Basingstoke: Carfax Publishing, v. 13, n. 4, p. 263-268, 2004. 10.1080/09629350400003068 0962-9351 http://repositorio.unifesp.br/handle/11600/27841 WOS:000224975100005 |
dc.identifier.dark.fl_str_mv |
ark:/48912/001300000h9b6 |
url |
http://dx.doi.org/10.1080/09629350400003068 http://repositorio.unifesp.br/handle/11600/27841 |
identifier_str_mv |
Mediators of Inflammation. Basingstoke: Carfax Publishing, v. 13, n. 4, p. 263-268, 2004. 10.1080/09629350400003068 0962-9351 WOS:000224975100005 ark:/48912/001300000h9b6 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Mediators of Inflammation |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
263-268 |
dc.publisher.none.fl_str_mv |
Carfax Publishing |
publisher.none.fl_str_mv |
Carfax Publishing |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1818602465846624256 |