Effects of 'casoparan', a peptide isolated from casein hydrolysates with mastoparan-like properties

Detalhes bibliográficos
Autor(a) principal: Lebrun, I
Data de Publicação: 2004
Outros Autores: Cavallaro, V, Juliano, Luiz [UNIFESP], Juliano, Maria Aparecida [UNIFESP], Silva, MCCDE
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
dARK ID: ark:/48912/001300000h9b6
Texto Completo: http://dx.doi.org/10.1080/09629350400003068
http://repositorio.unifesp.br/handle/11600/27841
Resumo: CASEIN, a protein found in milk of several species, is divided into different chains from 19 to 25 kappaDa. Casein is also considered as a source of amino acids and generating peptides with biological activities such as opiate, immunostimulating, antibacterial, peptidase inhibitors, among others. in this work, Sephadex G-10 chromatography followed by high-performance liquid chromatography isolation purified NZCase TT, an industrial culture media for tetanus toxin production. in the first step, four pools were isolated and tested in different assays: isolated smooth muscle assay ( guinea pig ileum, rat uterus), phagocytosis in vitro of opsonized sheep red blood cells, and hydrogen peroxide ( H2O2) release from mouse peritoneal macrophages. Pool III was the main active pool being able to potentiate bradykinin action in guinea pig ileum, stimulating phagocitic activity by resident macrophages and increasing H2O2 release from macrophages previously activated with bacille Calmette Guerin. Using mass spectra the primary structure of the main peptide from pool III was obtained-INKKI, which corresponds to beta-casein fragment 26-30. the immunostimulating action is probably related to a direct action in macrophage cells.
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spelling Effects of 'casoparan', a peptide isolated from casein hydrolysates with mastoparan-like propertiesmastoparancasein peptidesinflammationmacrophagespeptide isolationCASEIN, a protein found in milk of several species, is divided into different chains from 19 to 25 kappaDa. Casein is also considered as a source of amino acids and generating peptides with biological activities such as opiate, immunostimulating, antibacterial, peptidase inhibitors, among others. in this work, Sephadex G-10 chromatography followed by high-performance liquid chromatography isolation purified NZCase TT, an industrial culture media for tetanus toxin production. in the first step, four pools were isolated and tested in different assays: isolated smooth muscle assay ( guinea pig ileum, rat uterus), phagocytosis in vitro of opsonized sheep red blood cells, and hydrogen peroxide ( H2O2) release from mouse peritoneal macrophages. Pool III was the main active pool being able to potentiate bradykinin action in guinea pig ileum, stimulating phagocitic activity by resident macrophages and increasing H2O2 release from macrophages previously activated with bacille Calmette Guerin. Using mass spectra the primary structure of the main peptide from pool III was obtained-INKKI, which corresponds to beta-casein fragment 26-30. the immunostimulating action is probably related to a direct action in macrophage cells.Butantan Inst, Biochem & Biophys Lab, São Paulo, BrazilButantan Inst, Physiopathol Lab, São Paulo, BrazilUNIFESP, Dept Biophys, São Paulo, BrazilUNIFESP, Dept Biophys, São Paulo, BrazilWeb of ScienceCarfax PublishingButantan InstUniversidade Federal de São Paulo (UNIFESP)Lebrun, ICavallaro, VJuliano, Luiz [UNIFESP]Juliano, Maria Aparecida [UNIFESP]Silva, MCCDE2016-01-24T12:37:16Z2016-01-24T12:37:16Z2004-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion263-268http://dx.doi.org/10.1080/09629350400003068Mediators of Inflammation. Basingstoke: Carfax Publishing, v. 13, n. 4, p. 263-268, 2004.10.1080/096293504000030680962-9351http://repositorio.unifesp.br/handle/11600/27841WOS:000224975100005ark:/48912/001300000h9b6engMediators of Inflammationinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2021-09-29T11:23:43Zoai:repositorio.unifesp.br/:11600/27841Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T20:18:48.363825Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Effects of 'casoparan', a peptide isolated from casein hydrolysates with mastoparan-like properties
title Effects of 'casoparan', a peptide isolated from casein hydrolysates with mastoparan-like properties
spellingShingle Effects of 'casoparan', a peptide isolated from casein hydrolysates with mastoparan-like properties
Lebrun, I
mastoparan
casein peptides
inflammation
macrophages
peptide isolation
title_short Effects of 'casoparan', a peptide isolated from casein hydrolysates with mastoparan-like properties
title_full Effects of 'casoparan', a peptide isolated from casein hydrolysates with mastoparan-like properties
title_fullStr Effects of 'casoparan', a peptide isolated from casein hydrolysates with mastoparan-like properties
title_full_unstemmed Effects of 'casoparan', a peptide isolated from casein hydrolysates with mastoparan-like properties
title_sort Effects of 'casoparan', a peptide isolated from casein hydrolysates with mastoparan-like properties
author Lebrun, I
author_facet Lebrun, I
Cavallaro, V
Juliano, Luiz [UNIFESP]
Juliano, Maria Aparecida [UNIFESP]
Silva, MCCDE
author_role author
author2 Cavallaro, V
Juliano, Luiz [UNIFESP]
Juliano, Maria Aparecida [UNIFESP]
Silva, MCCDE
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Butantan Inst
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Lebrun, I
Cavallaro, V
Juliano, Luiz [UNIFESP]
Juliano, Maria Aparecida [UNIFESP]
Silva, MCCDE
dc.subject.por.fl_str_mv mastoparan
casein peptides
inflammation
macrophages
peptide isolation
topic mastoparan
casein peptides
inflammation
macrophages
peptide isolation
description CASEIN, a protein found in milk of several species, is divided into different chains from 19 to 25 kappaDa. Casein is also considered as a source of amino acids and generating peptides with biological activities such as opiate, immunostimulating, antibacterial, peptidase inhibitors, among others. in this work, Sephadex G-10 chromatography followed by high-performance liquid chromatography isolation purified NZCase TT, an industrial culture media for tetanus toxin production. in the first step, four pools were isolated and tested in different assays: isolated smooth muscle assay ( guinea pig ileum, rat uterus), phagocytosis in vitro of opsonized sheep red blood cells, and hydrogen peroxide ( H2O2) release from mouse peritoneal macrophages. Pool III was the main active pool being able to potentiate bradykinin action in guinea pig ileum, stimulating phagocitic activity by resident macrophages and increasing H2O2 release from macrophages previously activated with bacille Calmette Guerin. Using mass spectra the primary structure of the main peptide from pool III was obtained-INKKI, which corresponds to beta-casein fragment 26-30. the immunostimulating action is probably related to a direct action in macrophage cells.
publishDate 2004
dc.date.none.fl_str_mv 2004-08-01
2016-01-24T12:37:16Z
2016-01-24T12:37:16Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1080/09629350400003068
Mediators of Inflammation. Basingstoke: Carfax Publishing, v. 13, n. 4, p. 263-268, 2004.
10.1080/09629350400003068
0962-9351
http://repositorio.unifesp.br/handle/11600/27841
WOS:000224975100005
dc.identifier.dark.fl_str_mv ark:/48912/001300000h9b6
url http://dx.doi.org/10.1080/09629350400003068
http://repositorio.unifesp.br/handle/11600/27841
identifier_str_mv Mediators of Inflammation. Basingstoke: Carfax Publishing, v. 13, n. 4, p. 263-268, 2004.
10.1080/09629350400003068
0962-9351
WOS:000224975100005
ark:/48912/001300000h9b6
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Mediators of Inflammation
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 263-268
dc.publisher.none.fl_str_mv Carfax Publishing
publisher.none.fl_str_mv Carfax Publishing
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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