Clinical implications of autoantibodies in HIV infection
Autor(a) principal: | |
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Data de Publicação: | 1997 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/25816 http://dx.doi.org/10.1097/00002030-199715000-00009 |
Resumo: | Objective: To study the frequency and specificity of autoantibodies in HIV-infected subjects and their association with rheumatic manifestations, immunodeficiency, and prognosis.Design: Prospective study of sequentially selected HIV-infected patients. Indirect immunofluorescence reading was performed by two independent observers blinded for the patient diagnosis. Enzyme-linked immunosorbent assay (ELISA) was performed using coded serum samples.Setting: the study was performed at the Infectious Disease and Rheumatology Divisions of a tertiary care university hospital.Patients: One hundred sequentially selected HIV-infected patients formed group A. Controls included 80 non-HIV-infected high-risk individuals (group B), 20 herpesvirus-infected patients (group C), and 30 healthy blood donors (group D).Main outcome measures: Patients were followed for 2 years and evaluated for the presence of immunodeficiency, rheumatic manifestations, circulating autoantibodies and total CD4+ cell count. Indirect immunofluorescence was used to investigate antinuclear antibodies, antibodies to native DNA, smooth muscle, parietal cell, glomeruli, thyroid, and neutrophil cytoplasm. Agglutination was used to detect antibodies to erythrocytes and rheumatoid factor. ELISA was used to determine antibodies to cardiolipin and denatured DNA. CD4+ lymphocytes were counted by flow cytometry. Immunoglobulin (Ig) G, IgM and IgA serum levels were determined by radial immunodiffusion.Results: HIV-infected patients presented higher overall frequency of autoantibodies than the other groups. No difference was observed between immunodeficient and asymptomatic HIV-infected patients. the most frequent specificities were antibodies to cardiolipin and to denatured DNA. Ig serum levels did not correlate with the occurrence of autoantibodies. the presence of autoantibodies was associated with lower CD4+ cell counts and with higher mortality within 2 years. Rheumatic manifestations were observed in 35 HIV-infected patients and were not associated with the occurrence of autoantibodies or the presence of immunodeficiency.Conclusions: HIV infection is associated with an increased incidence of autoantibodies. Although not related to the occurrence of rheumatic manifestations, the presence of autoantibodies was significantly associated with lower CD4+ lymphocyte counts and increased mortality, which implies prognostic significance to this phenomenon in the context of HIV infection. |
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Massaki, Paulo Sergio [UNIFESP]Accetturi, Conceicao Aparecida [UNIFESP]Nishie, I. A.Silva, Neusa Pereira da [UNIFESP]Sato, Emília Inoue [UNIFESP]Andrade, Luís E. C [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2016-01-24T12:30:29Z2016-01-24T12:30:29Z1997-12-01Aids. Philadelphia: Lippincott Williams & Wilkins, v. 11, n. 15, p. 1845-1850, 1997.0269-9370http://repositorio.unifesp.br/handle/11600/25816http://dx.doi.org/10.1097/00002030-199715000-0000910.1097/00002030-199715000-00009WOS:A1997YH35800009Objective: To study the frequency and specificity of autoantibodies in HIV-infected subjects and their association with rheumatic manifestations, immunodeficiency, and prognosis.Design: Prospective study of sequentially selected HIV-infected patients. Indirect immunofluorescence reading was performed by two independent observers blinded for the patient diagnosis. Enzyme-linked immunosorbent assay (ELISA) was performed using coded serum samples.Setting: the study was performed at the Infectious Disease and Rheumatology Divisions of a tertiary care university hospital.Patients: One hundred sequentially selected HIV-infected patients formed group A. Controls included 80 non-HIV-infected high-risk individuals (group B), 20 herpesvirus-infected patients (group C), and 30 healthy blood donors (group D).Main outcome measures: Patients were followed for 2 years and evaluated for the presence of immunodeficiency, rheumatic manifestations, circulating autoantibodies and total CD4+ cell count. Indirect immunofluorescence was used to investigate antinuclear antibodies, antibodies to native DNA, smooth muscle, parietal cell, glomeruli, thyroid, and neutrophil cytoplasm. Agglutination was used to detect antibodies to erythrocytes and rheumatoid factor. ELISA was used to determine antibodies to cardiolipin and denatured DNA. CD4+ lymphocytes were counted by flow cytometry. Immunoglobulin (Ig) G, IgM and IgA serum levels were determined by radial immunodiffusion.Results: HIV-infected patients presented higher overall frequency of autoantibodies than the other groups. No difference was observed between immunodeficient and asymptomatic HIV-infected patients. the most frequent specificities were antibodies to cardiolipin and to denatured DNA. Ig serum levels did not correlate with the occurrence of autoantibodies. the presence of autoantibodies was associated with lower CD4+ cell counts and with higher mortality within 2 years. Rheumatic manifestations were observed in 35 HIV-infected patients and were not associated with the occurrence of autoantibodies or the presence of immunodeficiency.Conclusions: HIV infection is associated with an increased incidence of autoantibodies. Although not related to the occurrence of rheumatic manifestations, the presence of autoantibodies was significantly associated with lower CD4+ lymphocyte counts and increased mortality, which implies prognostic significance to this phenomenon in the context of HIV infection.Universidade Federal de São Paulo, DIV RHEUMATOL, ESCOLA PAULISTA MED, BR-04023900 São Paulo, BRAZILUniversidade Federal de São Paulo, DIV INFECT DIS, ESCOLA PAULISTA MED, BR-04023900 São Paulo, BRAZILUniversidade Federal de São Paulo, DIV RHEUMATOL, ESCOLA PAULISTA MED, BR-04023900 São Paulo, BRAZILUniversidade Federal de São Paulo, DIV INFECT DIS, ESCOLA PAULISTA MED, BR-04023900 São Paulo, BRAZILWeb of Science1845-1850engLippincott Williams & WilkinsAidsAIDSautoantibodiesantinuclear antibodiesClinical implications of autoantibodies in HIV infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/258162023-01-30 22:20:24.592metadata only accessoai:repositorio.unifesp.br:11600/25816Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-01-31T01:20:24Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Clinical implications of autoantibodies in HIV infection |
title |
Clinical implications of autoantibodies in HIV infection |
spellingShingle |
Clinical implications of autoantibodies in HIV infection Massaki, Paulo Sergio [UNIFESP] AIDS autoantibodies antinuclear antibodies |
title_short |
Clinical implications of autoantibodies in HIV infection |
title_full |
Clinical implications of autoantibodies in HIV infection |
title_fullStr |
Clinical implications of autoantibodies in HIV infection |
title_full_unstemmed |
Clinical implications of autoantibodies in HIV infection |
title_sort |
Clinical implications of autoantibodies in HIV infection |
author |
Massaki, Paulo Sergio [UNIFESP] |
author_facet |
Massaki, Paulo Sergio [UNIFESP] Accetturi, Conceicao Aparecida [UNIFESP] Nishie, I. A. Silva, Neusa Pereira da [UNIFESP] Sato, Emília Inoue [UNIFESP] Andrade, Luís E. C [UNIFESP] |
author_role |
author |
author2 |
Accetturi, Conceicao Aparecida [UNIFESP] Nishie, I. A. Silva, Neusa Pereira da [UNIFESP] Sato, Emília Inoue [UNIFESP] Andrade, Luís E. C [UNIFESP] |
author2_role |
author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Massaki, Paulo Sergio [UNIFESP] Accetturi, Conceicao Aparecida [UNIFESP] Nishie, I. A. Silva, Neusa Pereira da [UNIFESP] Sato, Emília Inoue [UNIFESP] Andrade, Luís E. C [UNIFESP] |
dc.subject.eng.fl_str_mv |
AIDS autoantibodies antinuclear antibodies |
topic |
AIDS autoantibodies antinuclear antibodies |
description |
Objective: To study the frequency and specificity of autoantibodies in HIV-infected subjects and their association with rheumatic manifestations, immunodeficiency, and prognosis.Design: Prospective study of sequentially selected HIV-infected patients. Indirect immunofluorescence reading was performed by two independent observers blinded for the patient diagnosis. Enzyme-linked immunosorbent assay (ELISA) was performed using coded serum samples.Setting: the study was performed at the Infectious Disease and Rheumatology Divisions of a tertiary care university hospital.Patients: One hundred sequentially selected HIV-infected patients formed group A. Controls included 80 non-HIV-infected high-risk individuals (group B), 20 herpesvirus-infected patients (group C), and 30 healthy blood donors (group D).Main outcome measures: Patients were followed for 2 years and evaluated for the presence of immunodeficiency, rheumatic manifestations, circulating autoantibodies and total CD4+ cell count. Indirect immunofluorescence was used to investigate antinuclear antibodies, antibodies to native DNA, smooth muscle, parietal cell, glomeruli, thyroid, and neutrophil cytoplasm. Agglutination was used to detect antibodies to erythrocytes and rheumatoid factor. ELISA was used to determine antibodies to cardiolipin and denatured DNA. CD4+ lymphocytes were counted by flow cytometry. Immunoglobulin (Ig) G, IgM and IgA serum levels were determined by radial immunodiffusion.Results: HIV-infected patients presented higher overall frequency of autoantibodies than the other groups. No difference was observed between immunodeficient and asymptomatic HIV-infected patients. the most frequent specificities were antibodies to cardiolipin and to denatured DNA. Ig serum levels did not correlate with the occurrence of autoantibodies. the presence of autoantibodies was associated with lower CD4+ cell counts and with higher mortality within 2 years. Rheumatic manifestations were observed in 35 HIV-infected patients and were not associated with the occurrence of autoantibodies or the presence of immunodeficiency.Conclusions: HIV infection is associated with an increased incidence of autoantibodies. Although not related to the occurrence of rheumatic manifestations, the presence of autoantibodies was significantly associated with lower CD4+ lymphocyte counts and increased mortality, which implies prognostic significance to this phenomenon in the context of HIV infection. |
publishDate |
1997 |
dc.date.issued.fl_str_mv |
1997-12-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T12:30:29Z |
dc.date.available.fl_str_mv |
2016-01-24T12:30:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Aids. Philadelphia: Lippincott Williams & Wilkins, v. 11, n. 15, p. 1845-1850, 1997. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/25816 http://dx.doi.org/10.1097/00002030-199715000-00009 |
dc.identifier.issn.none.fl_str_mv |
0269-9370 |
dc.identifier.doi.none.fl_str_mv |
10.1097/00002030-199715000-00009 |
dc.identifier.wos.none.fl_str_mv |
WOS:A1997YH35800009 |
identifier_str_mv |
Aids. Philadelphia: Lippincott Williams & Wilkins, v. 11, n. 15, p. 1845-1850, 1997. 0269-9370 10.1097/00002030-199715000-00009 WOS:A1997YH35800009 |
url |
http://repositorio.unifesp.br/handle/11600/25816 http://dx.doi.org/10.1097/00002030-199715000-00009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Aids |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1845-1850 |
dc.publisher.none.fl_str_mv |
Lippincott Williams & Wilkins |
publisher.none.fl_str_mv |
Lippincott Williams & Wilkins |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764281773555712 |