Frequency of Werner helicase 1367 polymorphism and age-related morbidity in an elderly Brazilian population
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1590/S0100-879X2005000700008 http://repositorio.unifesp.br/handle/11600/2592 |
Resumo: | Werner syndrome (WS) is a premature aging disease caused by a mutation in the WRN gene. The gene was identified in 1996 and its product acts as a DNA helicase and exonuclease. Some specific WRN polymorphic variants were associated with increased risk for cardiovascular diseases. The identification of genetic polymorphisms as risk factors for complex diseases affecting older people can improve their prevention, diagnosis and prognosis. We investigated WRN codon 1367 polymorphism in 383 residents in a district of the city of São Paulo, who were enrolled in an Elderly Brazilian Longitudinal Study. Their mean age was 79.70 ± 5.32 years, ranging from 67 to 97. This population was composed of 262 females (68.4%) and 121 males (31.6%) of European (89.2%), Japanese (3.3%), Middle Eastern (1.81%), and mixed and/or other origins (5.7%). There are no studies concerning this polymorphism in Brazilian population. These subjects were evaluated clinically every two years. The major health problems and morbidities affecting this cohort were cardiovascular diseases (21.7%), hypertension (83.7%), diabetes (63.3%), obesity (41.23%), dementia (8.0%), depression (20.0%), and neoplasia (10.8%). Their prevalence is similar to some urban elderly Brazilian samples. DNA was isolated from blood cells, amplified by PCR and digested with PmaCI. Allele frequencies were 0.788 for the cysteine and 0.211 for the arginine. Genotype distributions were within that expected for the Hardy-Weinberg equilibrium. Female gender was associated with hypertension and obesity. Logistic regression analysis did not detect significant association between the polymorphism and morbidity. These findings confirm those from Europeans and differ from Japanese population. |
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Frequency of Werner helicase 1367 polymorphism and age-related morbidity in an elderly Brazilian populationWRN: codon 1367 polymorphismAge-related morbiditiesElderly cohort studyWerner syndrome (WS) is a premature aging disease caused by a mutation in the WRN gene. The gene was identified in 1996 and its product acts as a DNA helicase and exonuclease. Some specific WRN polymorphic variants were associated with increased risk for cardiovascular diseases. The identification of genetic polymorphisms as risk factors for complex diseases affecting older people can improve their prevention, diagnosis and prognosis. We investigated WRN codon 1367 polymorphism in 383 residents in a district of the city of São Paulo, who were enrolled in an Elderly Brazilian Longitudinal Study. Their mean age was 79.70 ± 5.32 years, ranging from 67 to 97. This population was composed of 262 females (68.4%) and 121 males (31.6%) of European (89.2%), Japanese (3.3%), Middle Eastern (1.81%), and mixed and/or other origins (5.7%). There are no studies concerning this polymorphism in Brazilian population. These subjects were evaluated clinically every two years. The major health problems and morbidities affecting this cohort were cardiovascular diseases (21.7%), hypertension (83.7%), diabetes (63.3%), obesity (41.23%), dementia (8.0%), depression (20.0%), and neoplasia (10.8%). Their prevalence is similar to some urban elderly Brazilian samples. DNA was isolated from blood cells, amplified by PCR and digested with PmaCI. Allele frequencies were 0.788 for the cysteine and 0.211 for the arginine. Genotype distributions were within that expected for the Hardy-Weinberg equilibrium. Female gender was associated with hypertension and obesity. Logistic regression analysis did not detect significant association between the polymorphism and morbidity. These findings confirm those from Europeans and differ from Japanese population.Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de MorfologiaUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Clínica MédicaUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Medicina PreventivaUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de PsiquiatriaFaculdade de Medicina de Marília Hemocentro Disciplina de Genética e Biologia MolecularUNIFESP, EPM, Depto. de MorfologiaUNIFESP, EPM, Depto. de Clínica MédicaUNIFESP, EPM, Depto. de Medicina PreventivaUNIFESP, EPM, Depto. de PsiquiatriaSciELOAssociação Brasileira de Divulgação CientíficaUniversidade Federal de São Paulo (UNIFESP)Faculdade de Medicina de Marília Hemocentro Disciplina de Genética e Biologia MolecularSmith, Marilia de Arruda Cardoso [UNIFESP]Silva, M.d.a. [UNIFESP]Araujo, Lara Miguel Quirino [UNIFESP]Ramos, Luiz Roberto [UNIFESP]Lábio, Roger Willian deBurbano, Rommel Rodríguez [UNIFESP]Peres, Clovis de Araujo [UNIFESP]Andreoli, Sergio Baxter [UNIFESP]Payão, Spencer Luiz Marques [UNIFESP]Cendoroglo, Maysa Seabra [UNIFESP]2015-06-14T13:31:39Z2015-06-14T13:31:39Z2005-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1053-1059application/pdfhttp://dx.doi.org/10.1590/S0100-879X2005000700008Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 38, n. 7, p. 1053-1059, 2005.10.1590/S0100-879X2005000700008S0100-879X2005000700008.pdf0100-879XS0100-879X2005000700008http://repositorio.unifesp.br/handle/11600/2592WOS:000230829900008engBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-05T23:17:02Zoai:repositorio.unifesp.br/:11600/2592Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-05T23:17:02Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Frequency of Werner helicase 1367 polymorphism and age-related morbidity in an elderly Brazilian population |
title |
Frequency of Werner helicase 1367 polymorphism and age-related morbidity in an elderly Brazilian population |
spellingShingle |
Frequency of Werner helicase 1367 polymorphism and age-related morbidity in an elderly Brazilian population Smith, Marilia de Arruda Cardoso [UNIFESP] WRN: codon 1367 polymorphism Age-related morbidities Elderly cohort study |
title_short |
Frequency of Werner helicase 1367 polymorphism and age-related morbidity in an elderly Brazilian population |
title_full |
Frequency of Werner helicase 1367 polymorphism and age-related morbidity in an elderly Brazilian population |
title_fullStr |
Frequency of Werner helicase 1367 polymorphism and age-related morbidity in an elderly Brazilian population |
title_full_unstemmed |
Frequency of Werner helicase 1367 polymorphism and age-related morbidity in an elderly Brazilian population |
title_sort |
Frequency of Werner helicase 1367 polymorphism and age-related morbidity in an elderly Brazilian population |
author |
Smith, Marilia de Arruda Cardoso [UNIFESP] |
author_facet |
Smith, Marilia de Arruda Cardoso [UNIFESP] Silva, M.d.a. [UNIFESP] Araujo, Lara Miguel Quirino [UNIFESP] Ramos, Luiz Roberto [UNIFESP] Lábio, Roger Willian de Burbano, Rommel Rodríguez [UNIFESP] Peres, Clovis de Araujo [UNIFESP] Andreoli, Sergio Baxter [UNIFESP] Payão, Spencer Luiz Marques [UNIFESP] Cendoroglo, Maysa Seabra [UNIFESP] |
author_role |
author |
author2 |
Silva, M.d.a. [UNIFESP] Araujo, Lara Miguel Quirino [UNIFESP] Ramos, Luiz Roberto [UNIFESP] Lábio, Roger Willian de Burbano, Rommel Rodríguez [UNIFESP] Peres, Clovis de Araujo [UNIFESP] Andreoli, Sergio Baxter [UNIFESP] Payão, Spencer Luiz Marques [UNIFESP] Cendoroglo, Maysa Seabra [UNIFESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Faculdade de Medicina de Marília Hemocentro Disciplina de Genética e Biologia Molecular |
dc.contributor.author.fl_str_mv |
Smith, Marilia de Arruda Cardoso [UNIFESP] Silva, M.d.a. [UNIFESP] Araujo, Lara Miguel Quirino [UNIFESP] Ramos, Luiz Roberto [UNIFESP] Lábio, Roger Willian de Burbano, Rommel Rodríguez [UNIFESP] Peres, Clovis de Araujo [UNIFESP] Andreoli, Sergio Baxter [UNIFESP] Payão, Spencer Luiz Marques [UNIFESP] Cendoroglo, Maysa Seabra [UNIFESP] |
dc.subject.por.fl_str_mv |
WRN: codon 1367 polymorphism Age-related morbidities Elderly cohort study |
topic |
WRN: codon 1367 polymorphism Age-related morbidities Elderly cohort study |
description |
Werner syndrome (WS) is a premature aging disease caused by a mutation in the WRN gene. The gene was identified in 1996 and its product acts as a DNA helicase and exonuclease. Some specific WRN polymorphic variants were associated with increased risk for cardiovascular diseases. The identification of genetic polymorphisms as risk factors for complex diseases affecting older people can improve their prevention, diagnosis and prognosis. We investigated WRN codon 1367 polymorphism in 383 residents in a district of the city of São Paulo, who were enrolled in an Elderly Brazilian Longitudinal Study. Their mean age was 79.70 ± 5.32 years, ranging from 67 to 97. This population was composed of 262 females (68.4%) and 121 males (31.6%) of European (89.2%), Japanese (3.3%), Middle Eastern (1.81%), and mixed and/or other origins (5.7%). There are no studies concerning this polymorphism in Brazilian population. These subjects were evaluated clinically every two years. The major health problems and morbidities affecting this cohort were cardiovascular diseases (21.7%), hypertension (83.7%), diabetes (63.3%), obesity (41.23%), dementia (8.0%), depression (20.0%), and neoplasia (10.8%). Their prevalence is similar to some urban elderly Brazilian samples. DNA was isolated from blood cells, amplified by PCR and digested with PmaCI. Allele frequencies were 0.788 for the cysteine and 0.211 for the arginine. Genotype distributions were within that expected for the Hardy-Weinberg equilibrium. Female gender was associated with hypertension and obesity. Logistic regression analysis did not detect significant association between the polymorphism and morbidity. These findings confirm those from Europeans and differ from Japanese population. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-07-01 2015-06-14T13:31:39Z 2015-06-14T13:31:39Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-879X2005000700008 Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 38, n. 7, p. 1053-1059, 2005. 10.1590/S0100-879X2005000700008 S0100-879X2005000700008.pdf 0100-879X S0100-879X2005000700008 http://repositorio.unifesp.br/handle/11600/2592 WOS:000230829900008 |
url |
http://dx.doi.org/10.1590/S0100-879X2005000700008 http://repositorio.unifesp.br/handle/11600/2592 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 38, n. 7, p. 1053-1059, 2005. 10.1590/S0100-879X2005000700008 S0100-879X2005000700008.pdf 0100-879X S0100-879X2005000700008 WOS:000230829900008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1053-1059 application/pdf |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268367668772864 |