Application of four molecular typing methods for analysis of Mycobacterium fortuitum group strains causing post-mammaplasty infections
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1111/j.1469-0691.2005.01312.x http://repositorio.unifesp.br/handle/11600/28709 |
Resumo: | A cluster of cases of post-augmentation mammaplasty surgical site infections occurred between 2002 and 2004 in Campinas, in the southern region of Brazil. Rapidly growing mycobacteria were isolated from samples from 12 patients. Eleven isolates were identified as Mycobacterium fortuitum and one as Mycobacterium porcinum by PCR-restriction digestion of the hsp65 gene. These 12 isolates, plus six additional M. fortuitum isolates from non-related patients, were typed by pulsed-field gel electrophoresis (PFGE) and three PCR-based techniques: 16S-23S rRNA internal transcribed spacer (ITS) genotyping; randomly amplified polymorphic DNA (RAPD) PCR; and enterobacterial repetitive intergenic consensus (ERIC) PCR. Four novel M. fortuitum allelic variants were identified by restriction analysis of the ITS fragment. One major cluster, comprising six M. fortuitum isolates, and a second cluster of two isolates, were identified by the four methods. RAPD-PCR and ITS genotyping were less discriminative than ERIC-PCR. ERIC-PCR was comparable to PFGE as a valuable complementary tool for investigation of this type of outbreak. |
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Application of four molecular typing methods for analysis of Mycobacterium fortuitum group strains causing post-mammaplasty infectionsinfectionsmammaplastymolecular epidemiologyMycobacterium fortuitumplastic surgerytypingA cluster of cases of post-augmentation mammaplasty surgical site infections occurred between 2002 and 2004 in Campinas, in the southern region of Brazil. Rapidly growing mycobacteria were isolated from samples from 12 patients. Eleven isolates were identified as Mycobacterium fortuitum and one as Mycobacterium porcinum by PCR-restriction digestion of the hsp65 gene. These 12 isolates, plus six additional M. fortuitum isolates from non-related patients, were typed by pulsed-field gel electrophoresis (PFGE) and three PCR-based techniques: 16S-23S rRNA internal transcribed spacer (ITS) genotyping; randomly amplified polymorphic DNA (RAPD) PCR; and enterobacterial repetitive intergenic consensus (ERIC) PCR. Four novel M. fortuitum allelic variants were identified by restriction analysis of the ITS fragment. One major cluster, comprising six M. fortuitum isolates, and a second cluster of two isolates, were identified by the four methods. RAPD-PCR and ITS genotyping were less discriminative than ERIC-PCR. ERIC-PCR was comparable to PFGE as a valuable complementary tool for investigation of this type of outbreak.Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, SP, BrazilFleury Ctr Med Diagnost, São Paulo, BrazilInst Adolfo Lutz Registro, São Paulo, BrazilInst Vozza Med & Diagnose LTDA, Campinas, BrazilCtr Vigilancia Epidemiol Prof Alexandre Vranjac, São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, SP, BrazilWeb of ScienceBlackwell PublishingUniversidade Federal de São Paulo (UNIFESP)Fleury Ctr Med DiagnostInst Adolfo Lutz RegistroInst Vozza Med & Diagnose LTDACtr Vigilancia Epidemiol Prof Alexandre VranjacSampaio, JLM [UNIFESP]Chimara, EricaFerrazoli, LucilaineTelles, Maria Alice da SilvaDel Guercio, VMFJerico, ZVNMiyashiro, Kozue [UNIFESP]Fortaleza, CMCBPadoveze, M. C.Leao, S. C. [UNIFESP]2016-01-24T12:40:56Z2016-01-24T12:40:56Z2006-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion142-149application/pdfhttp://dx.doi.org/10.1111/j.1469-0691.2005.01312.xClinical Microbiology and Infection. Oxford: Blackwell Publishing, v. 12, n. 2, p. 142-149, 2006.10.1111/j.1469-0691.2005.01312.xWOS000234698700008.pdf1198-743Xhttp://repositorio.unifesp.br/handle/11600/28709WOS:000234698700008engClinical Microbiology and Infectioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-10-10T10:29:50Zoai:repositorio.unifesp.br/:11600/28709Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-10-10T10:29:50Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Application of four molecular typing methods for analysis of Mycobacterium fortuitum group strains causing post-mammaplasty infections |
title |
Application of four molecular typing methods for analysis of Mycobacterium fortuitum group strains causing post-mammaplasty infections |
spellingShingle |
Application of four molecular typing methods for analysis of Mycobacterium fortuitum group strains causing post-mammaplasty infections Sampaio, JLM [UNIFESP] infections mammaplasty molecular epidemiology Mycobacterium fortuitum plastic surgery typing |
title_short |
Application of four molecular typing methods for analysis of Mycobacterium fortuitum group strains causing post-mammaplasty infections |
title_full |
Application of four molecular typing methods for analysis of Mycobacterium fortuitum group strains causing post-mammaplasty infections |
title_fullStr |
Application of four molecular typing methods for analysis of Mycobacterium fortuitum group strains causing post-mammaplasty infections |
title_full_unstemmed |
Application of four molecular typing methods for analysis of Mycobacterium fortuitum group strains causing post-mammaplasty infections |
title_sort |
Application of four molecular typing methods for analysis of Mycobacterium fortuitum group strains causing post-mammaplasty infections |
author |
Sampaio, JLM [UNIFESP] |
author_facet |
Sampaio, JLM [UNIFESP] Chimara, Erica Ferrazoli, Lucilaine Telles, Maria Alice da Silva Del Guercio, VMF Jerico, ZVN Miyashiro, Kozue [UNIFESP] Fortaleza, CMCB Padoveze, M. C. Leao, S. C. [UNIFESP] |
author_role |
author |
author2 |
Chimara, Erica Ferrazoli, Lucilaine Telles, Maria Alice da Silva Del Guercio, VMF Jerico, ZVN Miyashiro, Kozue [UNIFESP] Fortaleza, CMCB Padoveze, M. C. Leao, S. C. [UNIFESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Fleury Ctr Med Diagnost Inst Adolfo Lutz Registro Inst Vozza Med & Diagnose LTDA Ctr Vigilancia Epidemiol Prof Alexandre Vranjac |
dc.contributor.author.fl_str_mv |
Sampaio, JLM [UNIFESP] Chimara, Erica Ferrazoli, Lucilaine Telles, Maria Alice da Silva Del Guercio, VMF Jerico, ZVN Miyashiro, Kozue [UNIFESP] Fortaleza, CMCB Padoveze, M. C. Leao, S. C. [UNIFESP] |
dc.subject.por.fl_str_mv |
infections mammaplasty molecular epidemiology Mycobacterium fortuitum plastic surgery typing |
topic |
infections mammaplasty molecular epidemiology Mycobacterium fortuitum plastic surgery typing |
description |
A cluster of cases of post-augmentation mammaplasty surgical site infections occurred between 2002 and 2004 in Campinas, in the southern region of Brazil. Rapidly growing mycobacteria were isolated from samples from 12 patients. Eleven isolates were identified as Mycobacterium fortuitum and one as Mycobacterium porcinum by PCR-restriction digestion of the hsp65 gene. These 12 isolates, plus six additional M. fortuitum isolates from non-related patients, were typed by pulsed-field gel electrophoresis (PFGE) and three PCR-based techniques: 16S-23S rRNA internal transcribed spacer (ITS) genotyping; randomly amplified polymorphic DNA (RAPD) PCR; and enterobacterial repetitive intergenic consensus (ERIC) PCR. Four novel M. fortuitum allelic variants were identified by restriction analysis of the ITS fragment. One major cluster, comprising six M. fortuitum isolates, and a second cluster of two isolates, were identified by the four methods. RAPD-PCR and ITS genotyping were less discriminative than ERIC-PCR. ERIC-PCR was comparable to PFGE as a valuable complementary tool for investigation of this type of outbreak. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-02-01 2016-01-24T12:40:56Z 2016-01-24T12:40:56Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/j.1469-0691.2005.01312.x Clinical Microbiology and Infection. Oxford: Blackwell Publishing, v. 12, n. 2, p. 142-149, 2006. 10.1111/j.1469-0691.2005.01312.x WOS000234698700008.pdf 1198-743X http://repositorio.unifesp.br/handle/11600/28709 WOS:000234698700008 |
url |
http://dx.doi.org/10.1111/j.1469-0691.2005.01312.x http://repositorio.unifesp.br/handle/11600/28709 |
identifier_str_mv |
Clinical Microbiology and Infection. Oxford: Blackwell Publishing, v. 12, n. 2, p. 142-149, 2006. 10.1111/j.1469-0691.2005.01312.x WOS000234698700008.pdf 1198-743X WOS:000234698700008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical Microbiology and Infection |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
142-149 application/pdf |
dc.publisher.none.fl_str_mv |
Blackwell Publishing |
publisher.none.fl_str_mv |
Blackwell Publishing |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268447492669440 |