Application of four molecular typing methods for analysis of Mycobacterium fortuitum group strains causing post-mammaplasty infections

Detalhes bibliográficos
Autor(a) principal: Sampaio, JLM [UNIFESP]
Data de Publicação: 2006
Outros Autores: Chimara, Erica, Ferrazoli, Lucilaine, Telles, Maria Alice da Silva, Del Guercio, VMF, Jerico, ZVN, Miyashiro, Kozue [UNIFESP], Fortaleza, CMCB, Padoveze, M. C., Leao, S. C. [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1111/j.1469-0691.2005.01312.x
http://repositorio.unifesp.br/handle/11600/28709
Resumo: A cluster of cases of post-augmentation mammaplasty surgical site infections occurred between 2002 and 2004 in Campinas, in the southern region of Brazil. Rapidly growing mycobacteria were isolated from samples from 12 patients. Eleven isolates were identified as Mycobacterium fortuitum and one as Mycobacterium porcinum by PCR-restriction digestion of the hsp65 gene. These 12 isolates, plus six additional M. fortuitum isolates from non-related patients, were typed by pulsed-field gel electrophoresis (PFGE) and three PCR-based techniques: 16S-23S rRNA internal transcribed spacer (ITS) genotyping; randomly amplified polymorphic DNA (RAPD) PCR; and enterobacterial repetitive intergenic consensus (ERIC) PCR. Four novel M. fortuitum allelic variants were identified by restriction analysis of the ITS fragment. One major cluster, comprising six M. fortuitum isolates, and a second cluster of two isolates, were identified by the four methods. RAPD-PCR and ITS genotyping were less discriminative than ERIC-PCR. ERIC-PCR was comparable to PFGE as a valuable complementary tool for investigation of this type of outbreak.
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spelling Application of four molecular typing methods for analysis of Mycobacterium fortuitum group strains causing post-mammaplasty infectionsinfectionsmammaplastymolecular epidemiologyMycobacterium fortuitumplastic surgerytypingA cluster of cases of post-augmentation mammaplasty surgical site infections occurred between 2002 and 2004 in Campinas, in the southern region of Brazil. Rapidly growing mycobacteria were isolated from samples from 12 patients. Eleven isolates were identified as Mycobacterium fortuitum and one as Mycobacterium porcinum by PCR-restriction digestion of the hsp65 gene. These 12 isolates, plus six additional M. fortuitum isolates from non-related patients, were typed by pulsed-field gel electrophoresis (PFGE) and three PCR-based techniques: 16S-23S rRNA internal transcribed spacer (ITS) genotyping; randomly amplified polymorphic DNA (RAPD) PCR; and enterobacterial repetitive intergenic consensus (ERIC) PCR. Four novel M. fortuitum allelic variants were identified by restriction analysis of the ITS fragment. One major cluster, comprising six M. fortuitum isolates, and a second cluster of two isolates, were identified by the four methods. RAPD-PCR and ITS genotyping were less discriminative than ERIC-PCR. ERIC-PCR was comparable to PFGE as a valuable complementary tool for investigation of this type of outbreak.Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, SP, BrazilFleury Ctr Med Diagnost, São Paulo, BrazilInst Adolfo Lutz Registro, São Paulo, BrazilInst Vozza Med & Diagnose LTDA, Campinas, BrazilCtr Vigilancia Epidemiol Prof Alexandre Vranjac, São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, SP, BrazilWeb of ScienceBlackwell PublishingUniversidade Federal de São Paulo (UNIFESP)Fleury Ctr Med DiagnostInst Adolfo Lutz RegistroInst Vozza Med & Diagnose LTDACtr Vigilancia Epidemiol Prof Alexandre VranjacSampaio, JLM [UNIFESP]Chimara, EricaFerrazoli, LucilaineTelles, Maria Alice da SilvaDel Guercio, VMFJerico, ZVNMiyashiro, Kozue [UNIFESP]Fortaleza, CMCBPadoveze, M. C.Leao, S. C. [UNIFESP]2016-01-24T12:40:56Z2016-01-24T12:40:56Z2006-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion142-149application/pdfhttp://dx.doi.org/10.1111/j.1469-0691.2005.01312.xClinical Microbiology and Infection. Oxford: Blackwell Publishing, v. 12, n. 2, p. 142-149, 2006.10.1111/j.1469-0691.2005.01312.xWOS000234698700008.pdf1198-743Xhttp://repositorio.unifesp.br/handle/11600/28709WOS:000234698700008engClinical Microbiology and Infectioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-10-10T10:29:50Zoai:repositorio.unifesp.br/:11600/28709Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-10-10T10:29:50Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Application of four molecular typing methods for analysis of Mycobacterium fortuitum group strains causing post-mammaplasty infections
title Application of four molecular typing methods for analysis of Mycobacterium fortuitum group strains causing post-mammaplasty infections
spellingShingle Application of four molecular typing methods for analysis of Mycobacterium fortuitum group strains causing post-mammaplasty infections
Sampaio, JLM [UNIFESP]
infections
mammaplasty
molecular epidemiology
Mycobacterium fortuitum
plastic surgery
typing
title_short Application of four molecular typing methods for analysis of Mycobacterium fortuitum group strains causing post-mammaplasty infections
title_full Application of four molecular typing methods for analysis of Mycobacterium fortuitum group strains causing post-mammaplasty infections
title_fullStr Application of four molecular typing methods for analysis of Mycobacterium fortuitum group strains causing post-mammaplasty infections
title_full_unstemmed Application of four molecular typing methods for analysis of Mycobacterium fortuitum group strains causing post-mammaplasty infections
title_sort Application of four molecular typing methods for analysis of Mycobacterium fortuitum group strains causing post-mammaplasty infections
author Sampaio, JLM [UNIFESP]
author_facet Sampaio, JLM [UNIFESP]
Chimara, Erica
Ferrazoli, Lucilaine
Telles, Maria Alice da Silva
Del Guercio, VMF
Jerico, ZVN
Miyashiro, Kozue [UNIFESP]
Fortaleza, CMCB
Padoveze, M. C.
Leao, S. C. [UNIFESP]
author_role author
author2 Chimara, Erica
Ferrazoli, Lucilaine
Telles, Maria Alice da Silva
Del Guercio, VMF
Jerico, ZVN
Miyashiro, Kozue [UNIFESP]
Fortaleza, CMCB
Padoveze, M. C.
Leao, S. C. [UNIFESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Fleury Ctr Med Diagnost
Inst Adolfo Lutz Registro
Inst Vozza Med & Diagnose LTDA
Ctr Vigilancia Epidemiol Prof Alexandre Vranjac
dc.contributor.author.fl_str_mv Sampaio, JLM [UNIFESP]
Chimara, Erica
Ferrazoli, Lucilaine
Telles, Maria Alice da Silva
Del Guercio, VMF
Jerico, ZVN
Miyashiro, Kozue [UNIFESP]
Fortaleza, CMCB
Padoveze, M. C.
Leao, S. C. [UNIFESP]
dc.subject.por.fl_str_mv infections
mammaplasty
molecular epidemiology
Mycobacterium fortuitum
plastic surgery
typing
topic infections
mammaplasty
molecular epidemiology
Mycobacterium fortuitum
plastic surgery
typing
description A cluster of cases of post-augmentation mammaplasty surgical site infections occurred between 2002 and 2004 in Campinas, in the southern region of Brazil. Rapidly growing mycobacteria were isolated from samples from 12 patients. Eleven isolates were identified as Mycobacterium fortuitum and one as Mycobacterium porcinum by PCR-restriction digestion of the hsp65 gene. These 12 isolates, plus six additional M. fortuitum isolates from non-related patients, were typed by pulsed-field gel electrophoresis (PFGE) and three PCR-based techniques: 16S-23S rRNA internal transcribed spacer (ITS) genotyping; randomly amplified polymorphic DNA (RAPD) PCR; and enterobacterial repetitive intergenic consensus (ERIC) PCR. Four novel M. fortuitum allelic variants were identified by restriction analysis of the ITS fragment. One major cluster, comprising six M. fortuitum isolates, and a second cluster of two isolates, were identified by the four methods. RAPD-PCR and ITS genotyping were less discriminative than ERIC-PCR. ERIC-PCR was comparable to PFGE as a valuable complementary tool for investigation of this type of outbreak.
publishDate 2006
dc.date.none.fl_str_mv 2006-02-01
2016-01-24T12:40:56Z
2016-01-24T12:40:56Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1111/j.1469-0691.2005.01312.x
Clinical Microbiology and Infection. Oxford: Blackwell Publishing, v. 12, n. 2, p. 142-149, 2006.
10.1111/j.1469-0691.2005.01312.x
WOS000234698700008.pdf
1198-743X
http://repositorio.unifesp.br/handle/11600/28709
WOS:000234698700008
url http://dx.doi.org/10.1111/j.1469-0691.2005.01312.x
http://repositorio.unifesp.br/handle/11600/28709
identifier_str_mv Clinical Microbiology and Infection. Oxford: Blackwell Publishing, v. 12, n. 2, p. 142-149, 2006.
10.1111/j.1469-0691.2005.01312.x
WOS000234698700008.pdf
1198-743X
WOS:000234698700008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Clinical Microbiology and Infection
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 142-149
application/pdf
dc.publisher.none.fl_str_mv Blackwell Publishing
publisher.none.fl_str_mv Blackwell Publishing
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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