A Recombinant Protein Based on Trypanosoma cruzi P21 Enhances Phagocytosis

• Autor(a) principal: Rodrigues, Adele A.
• Data de Publicação: 2012
• Outros Autores: Clemente, Tatiana M., Santos, Marlus A. dos, Machado, Fabricio C., Gomes, Rafael G. B., Moreira, Heline Hellen T., Cruz, Mario C. [UNIFESP], Brigido, Paula C., Santos, Paulo C. F. dos, Martins, Flavia A., Bahia, Diana [UNIFESP], Maricato, Juliana Terzi [UNIFESP], Janini, Luiz Mário Ramos [UNIFESP], Reboredo, Eduardo H., Mortara, Renato Arruda [UNIFESP], Silva, Claudio V. da
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.1371/journal.pone.0051384; http://repositorio.unifesp.br/handle/11600/35608

Background: P21 is a secreted protein expressed in all developmental stages of Trypanosoma cruzi. the aim of this study was to determine the effect of the recombinant protein based on P21 (P21-His(6)) on inflammatory macrophages during phagocytosis.Findings: Our results showed that P21-His(6) acts as a phagocytosis inducer by binding to CXCR4 chemokine receptor and activating actin polymerization in a way dependent on the PI3-kinase signaling pathway.Conclusions: Thus, our results shed light on the notion that native P21 is a component related to T. cruzi evasion from the immune response and that CXCR4 may be involved in phagocytosis. P21-His(6) represents an important experimental control tool to study phagocytosis signaling pathways of different intracellular parasites and particles.