The antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilms
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/8184 http://dx.doi.org/10.1590/S1806-83242013005000024 |
Resumo: | The aim of this study was to evaluate the effects of Citrus limonum and Citrus aurantium essential oils (EOs) compared to 0.2% chlorhexidine (CHX) and 1% sodium hypochlorite (NaOCl) on multi-species biofilms formed by Candida albicans, Enterococcus faecalis and Escherichia coli. The biofilms were grown in acrylic disks immersed in broth, inoculated with microbial suspension (106 cells/mL) and incubated at 37°C / 48 h. After the biofilms were formed, they were exposed for 5 minutes to the solutions (n = 10): C. aurantium EO, C. limonum EO, 0.2% CHX, 1% NaOCl or sterile saline solution [0.9% sodium chloride (NaCl)]. Next, the discs were placed in sterile 0.9% NaCl and sonicated to disperse the biofilms. Tenfold serial dilutions were performed and the aliquots were seeded onto selective agar and incubated at 37°C / 48 h. Next, the number of colony-forming units per milliliter was counted and analyzed statistically (Tukey test, p ≤ 0.05). C. aurantium EO and NaOCl inhibited the growth of all microorganisms in multi-species biofilms. C. limonum EO promoted a 100% reduction of C. albicans and E. coli, and 49.3% of E. faecalis. CHX was less effective against C. albicans and E. coli, yielding a reduction of 68.8% and 86.7%, respectively. However, the reduction of E. faecalis using CHX (81.7%) was greater than that obtained using C. limonum EO. Both Citrus limonum and Citrus aurantium EOs are effective in controlling multi-species biofilms; the microbial reductions achieved by EOs were not only similar to those of NaOCl, but even higher than those achieved by CHX, in some cases. |
id |
UFSP_b36bf4732cf64937f183843c31329503 |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br:11600/8184 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Oliveira, Sarah Almeida CoelhoZambrana, Jéssica Rabelo MinaDi Iorio, Fernanda Bispo ReisPereira, Cristiane AparecidaJorge, Antonio Olavo CardosoUniversidade Estadual Paulista (UNESP)Universidade Federal de São Paulo (UNIFESP)2015-06-14T13:46:52Z2015-06-14T13:46:52Z2014-01-01Brazilian Oral Research. Sociedade Brasileira de Pesquisa Odontológica - SBPqO, v. 28, n. 1, p. 22-27, 2014.1806-8324http://repositorio.unifesp.br/handle/11600/8184http://dx.doi.org/10.1590/S1806-83242013005000024S1806-83242014000100217.pdfS1806-8324201400010021710.1590/S1806-83242013005000024WOS:000328723700004The aim of this study was to evaluate the effects of Citrus limonum and Citrus aurantium essential oils (EOs) compared to 0.2% chlorhexidine (CHX) and 1% sodium hypochlorite (NaOCl) on multi-species biofilms formed by Candida albicans, Enterococcus faecalis and Escherichia coli. The biofilms were grown in acrylic disks immersed in broth, inoculated with microbial suspension (106 cells/mL) and incubated at 37°C / 48 h. After the biofilms were formed, they were exposed for 5 minutes to the solutions (n = 10): C. aurantium EO, C. limonum EO, 0.2% CHX, 1% NaOCl or sterile saline solution [0.9% sodium chloride (NaCl)]. Next, the discs were placed in sterile 0.9% NaCl and sonicated to disperse the biofilms. Tenfold serial dilutions were performed and the aliquots were seeded onto selective agar and incubated at 37°C / 48 h. Next, the number of colony-forming units per milliliter was counted and analyzed statistically (Tukey test, p ≤ 0.05). C. aurantium EO and NaOCl inhibited the growth of all microorganisms in multi-species biofilms. C. limonum EO promoted a 100% reduction of C. albicans and E. coli, and 49.3% of E. faecalis. CHX was less effective against C. albicans and E. coli, yielding a reduction of 68.8% and 86.7%, respectively. However, the reduction of E. faecalis using CHX (81.7%) was greater than that obtained using C. limonum EO. Both Citrus limonum and Citrus aurantium EOs are effective in controlling multi-species biofilms; the microbial reductions achieved by EOs were not only similar to those of NaOCl, but even higher than those achieved by CHX, in some cases.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista Institute of Science and Technology Department of Biosciences and Oral DiagnosisUniversidade Federal de São Paulo (UNIFESP) Institute of Environmental, Chemical and Pharmaceutics Sciences Department of Earth and Exact SciencesUNIFESP, Institute of Environmental, Chemical and Pharmaceutics Sciences Department of Earth and Exact SciencesFAPESP: 09/52048-1FAPESP: 2010/00879-4SciELO22-27engSociedade Brasileira de Pesquisa Odontológica - SBPqOBrazilian Oral ResearchBiofilmsCitrus limonumCitrus aurantiumThe antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilmsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS1806-83242014000100217.pdfapplication/pdf1782439${dspace.ui.url}/bitstream/11600/8184/1/S1806-83242014000100217.pdfc88bb6a3d5d1da890db5341dff4ec33aMD51open accessTEXTS1806-83242014000100217.pdf.txtS1806-83242014000100217.pdf.txtExtracted texttext/plain25186${dspace.ui.url}/bitstream/11600/8184/9/S1806-83242014000100217.pdf.txt7d897f5fafb888b917fa7895408559a3MD59open accessTHUMBNAILS1806-83242014000100217.pdf.jpgS1806-83242014000100217.pdf.jpgIM Thumbnailimage/jpeg6644${dspace.ui.url}/bitstream/11600/8184/11/S1806-83242014000100217.pdf.jpga4a9f118208267ee9e92748655eb2882MD511open access11600/81842023-06-05 19:32:16.323open accessoai:repositorio.unifesp.br:11600/8184Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:32:16Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
The antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilms |
title |
The antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilms |
spellingShingle |
The antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilms Oliveira, Sarah Almeida Coelho Biofilms Citrus limonum Citrus aurantium |
title_short |
The antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilms |
title_full |
The antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilms |
title_fullStr |
The antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilms |
title_full_unstemmed |
The antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilms |
title_sort |
The antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilms |
author |
Oliveira, Sarah Almeida Coelho |
author_facet |
Oliveira, Sarah Almeida Coelho Zambrana, Jéssica Rabelo Mina Di Iorio, Fernanda Bispo Reis Pereira, Cristiane Aparecida Jorge, Antonio Olavo Cardoso |
author_role |
author |
author2 |
Zambrana, Jéssica Rabelo Mina Di Iorio, Fernanda Bispo Reis Pereira, Cristiane Aparecida Jorge, Antonio Olavo Cardoso |
author2_role |
author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Oliveira, Sarah Almeida Coelho Zambrana, Jéssica Rabelo Mina Di Iorio, Fernanda Bispo Reis Pereira, Cristiane Aparecida Jorge, Antonio Olavo Cardoso |
dc.subject.eng.fl_str_mv |
Biofilms Citrus limonum Citrus aurantium |
topic |
Biofilms Citrus limonum Citrus aurantium |
description |
The aim of this study was to evaluate the effects of Citrus limonum and Citrus aurantium essential oils (EOs) compared to 0.2% chlorhexidine (CHX) and 1% sodium hypochlorite (NaOCl) on multi-species biofilms formed by Candida albicans, Enterococcus faecalis and Escherichia coli. The biofilms were grown in acrylic disks immersed in broth, inoculated with microbial suspension (106 cells/mL) and incubated at 37°C / 48 h. After the biofilms were formed, they were exposed for 5 minutes to the solutions (n = 10): C. aurantium EO, C. limonum EO, 0.2% CHX, 1% NaOCl or sterile saline solution [0.9% sodium chloride (NaCl)]. Next, the discs were placed in sterile 0.9% NaCl and sonicated to disperse the biofilms. Tenfold serial dilutions were performed and the aliquots were seeded onto selective agar and incubated at 37°C / 48 h. Next, the number of colony-forming units per milliliter was counted and analyzed statistically (Tukey test, p ≤ 0.05). C. aurantium EO and NaOCl inhibited the growth of all microorganisms in multi-species biofilms. C. limonum EO promoted a 100% reduction of C. albicans and E. coli, and 49.3% of E. faecalis. CHX was less effective against C. albicans and E. coli, yielding a reduction of 68.8% and 86.7%, respectively. However, the reduction of E. faecalis using CHX (81.7%) was greater than that obtained using C. limonum EO. Both Citrus limonum and Citrus aurantium EOs are effective in controlling multi-species biofilms; the microbial reductions achieved by EOs were not only similar to those of NaOCl, but even higher than those achieved by CHX, in some cases. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-01-01 |
dc.date.accessioned.fl_str_mv |
2015-06-14T13:46:52Z |
dc.date.available.fl_str_mv |
2015-06-14T13:46:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Brazilian Oral Research. Sociedade Brasileira de Pesquisa Odontológica - SBPqO, v. 28, n. 1, p. 22-27, 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/8184 http://dx.doi.org/10.1590/S1806-83242013005000024 |
dc.identifier.issn.none.fl_str_mv |
1806-8324 |
dc.identifier.file.none.fl_str_mv |
S1806-83242014000100217.pdf |
dc.identifier.scielo.none.fl_str_mv |
S1806-83242014000100217 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S1806-83242013005000024 |
dc.identifier.wos.none.fl_str_mv |
WOS:000328723700004 |
identifier_str_mv |
Brazilian Oral Research. Sociedade Brasileira de Pesquisa Odontológica - SBPqO, v. 28, n. 1, p. 22-27, 2014. 1806-8324 S1806-83242014000100217.pdf S1806-83242014000100217 10.1590/S1806-83242013005000024 WOS:000328723700004 |
url |
http://repositorio.unifesp.br/handle/11600/8184 http://dx.doi.org/10.1590/S1806-83242013005000024 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Brazilian Oral Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
22-27 |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
bitstream.url.fl_str_mv |
${dspace.ui.url}/bitstream/11600/8184/1/S1806-83242014000100217.pdf ${dspace.ui.url}/bitstream/11600/8184/9/S1806-83242014000100217.pdf.txt ${dspace.ui.url}/bitstream/11600/8184/11/S1806-83242014000100217.pdf.jpg |
bitstream.checksum.fl_str_mv |
c88bb6a3d5d1da890db5341dff4ec33a 7d897f5fafb888b917fa7895408559a3 a4a9f118208267ee9e92748655eb2882 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764256429473792 |