The antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilms
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1806-83242013005000024 http://hdl.handle.net/11449/109663 |
Resumo: | The aim of this study was to evaluate the effects of Citrus limonum and Citrus aurantium essential oils (EOs) compared to 0.2% chlorhexidine (CHX) and 1% sodium hypochlorite (NaOCl) on multi-species biofilms formed by Candida albicans, Enterococcus faecalis and Escherichia coli. The biofilms were grown in acrylic disks immersed in broth, inoculated with microbial suspension (106 cells/mL) and incubated at 37°C / 48 h. After the biofilms were formed, they were exposed for 5 minutes to the solutions (n = 10): C. aurantium EO, C. limonum EO, 0.2% CHX, 1% NaOCl or sterile saline solution [0.9% sodium chloride (NaCl)]. Next, the discs were placed in sterile 0.9% NaCl and sonicated to disperse the biofilms. Tenfold serial dilutions were performed and the aliquots were seeded onto selective agar and incubated at 37°C / 48 h. Next, the number of colony-forming units per milliliter was counted and analyzed statistically (Tukey test, p ≤ 0.05). C. aurantium EO and NaOCl inhibited the growth of all microorganisms in multi-species biofilms. C. limonum EO promoted a 100% reduction of C. albicans and E. coli, and 49.3% of E. faecalis. CHX was less effective against C. albicans and E. coli, yielding a reduction of 68.8% and 86.7%, respectively. However, the reduction of E. faecalis using CHX (81.7%) was greater than that obtained using C. limonum EO. Both Citrus limonum and Citrus aurantium EOs are effective in controlling multi-species biofilms; the microbial reductions achieved by EOs were not only similar to those of NaOCl, but even higher than those achieved by CHX, in some cases. |
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The antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilmsBiofilmsCitrus limonumCitrus aurantiumThe aim of this study was to evaluate the effects of Citrus limonum and Citrus aurantium essential oils (EOs) compared to 0.2% chlorhexidine (CHX) and 1% sodium hypochlorite (NaOCl) on multi-species biofilms formed by Candida albicans, Enterococcus faecalis and Escherichia coli. The biofilms were grown in acrylic disks immersed in broth, inoculated with microbial suspension (106 cells/mL) and incubated at 37°C / 48 h. After the biofilms were formed, they were exposed for 5 minutes to the solutions (n = 10): C. aurantium EO, C. limonum EO, 0.2% CHX, 1% NaOCl or sterile saline solution [0.9% sodium chloride (NaCl)]. Next, the discs were placed in sterile 0.9% NaCl and sonicated to disperse the biofilms. Tenfold serial dilutions were performed and the aliquots were seeded onto selective agar and incubated at 37°C / 48 h. Next, the number of colony-forming units per milliliter was counted and analyzed statistically (Tukey test, p ≤ 0.05). C. aurantium EO and NaOCl inhibited the growth of all microorganisms in multi-species biofilms. C. limonum EO promoted a 100% reduction of C. albicans and E. coli, and 49.3% of E. faecalis. CHX was less effective against C. albicans and E. coli, yielding a reduction of 68.8% and 86.7%, respectively. However, the reduction of E. faecalis using CHX (81.7%) was greater than that obtained using C. limonum EO. Both Citrus limonum and Citrus aurantium EOs are effective in controlling multi-species biofilms; the microbial reductions achieved by EOs were not only similar to those of NaOCl, but even higher than those achieved by CHX, in some cases.Universidade Estadual Paulista Institute of Science and Technology Department of Biosciences and Oral DiagnosisUniversidade Federal de São Paulo Institute of Environmental, Chemical and Pharmaceutics Sciences Department of Earth and Exact SciencesUniversidade Estadual Paulista Institute of Science and Technology Department of Biosciences and Oral DiagnosisSociedade Brasileira de Pesquisa Odontológica (SBPqO)Universidade Estadual Paulista (Unesp)Universidade Federal de São Paulo (UNIFESP)Oliveira, Sarah Almeida CoelhoZambrana, Jéssica Rabelo MinaDi Iorio, Fernanda Bispo ReisPereira, Cristiane AparecidaJorge, Antonio Olavo Cardoso [UNESP]2014-09-30T18:18:33Z2014-09-30T18:18:33Z2014-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article22-27application/pdfhttp://dx.doi.org/10.1590/S1806-83242013005000024Brazilian Oral Research. Sociedade Brasileira de Pesquisa Odontológica - SBPqO, v. 28, n. 1, p. 22-27, 2014.1806-8324http://hdl.handle.net/11449/10966310.1590/S1806-83242013005000024S1806-83242014000100217WOS:000328723700004S1806-83242014000100217.pdf0053567153623569SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Oral Researchinfo:eu-repo/semantics/openAccess2023-12-02T06:12:05Zoai:repositorio.unesp.br:11449/109663Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:16:48.311225Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilms |
title |
The antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilms |
spellingShingle |
The antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilms Oliveira, Sarah Almeida Coelho Biofilms Citrus limonum Citrus aurantium |
title_short |
The antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilms |
title_full |
The antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilms |
title_fullStr |
The antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilms |
title_full_unstemmed |
The antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilms |
title_sort |
The antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilms |
author |
Oliveira, Sarah Almeida Coelho |
author_facet |
Oliveira, Sarah Almeida Coelho Zambrana, Jéssica Rabelo Mina Di Iorio, Fernanda Bispo Reis Pereira, Cristiane Aparecida Jorge, Antonio Olavo Cardoso [UNESP] |
author_role |
author |
author2 |
Zambrana, Jéssica Rabelo Mina Di Iorio, Fernanda Bispo Reis Pereira, Cristiane Aparecida Jorge, Antonio Olavo Cardoso [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Oliveira, Sarah Almeida Coelho Zambrana, Jéssica Rabelo Mina Di Iorio, Fernanda Bispo Reis Pereira, Cristiane Aparecida Jorge, Antonio Olavo Cardoso [UNESP] |
dc.subject.por.fl_str_mv |
Biofilms Citrus limonum Citrus aurantium |
topic |
Biofilms Citrus limonum Citrus aurantium |
description |
The aim of this study was to evaluate the effects of Citrus limonum and Citrus aurantium essential oils (EOs) compared to 0.2% chlorhexidine (CHX) and 1% sodium hypochlorite (NaOCl) on multi-species biofilms formed by Candida albicans, Enterococcus faecalis and Escherichia coli. The biofilms were grown in acrylic disks immersed in broth, inoculated with microbial suspension (106 cells/mL) and incubated at 37°C / 48 h. After the biofilms were formed, they were exposed for 5 minutes to the solutions (n = 10): C. aurantium EO, C. limonum EO, 0.2% CHX, 1% NaOCl or sterile saline solution [0.9% sodium chloride (NaCl)]. Next, the discs were placed in sterile 0.9% NaCl and sonicated to disperse the biofilms. Tenfold serial dilutions were performed and the aliquots were seeded onto selective agar and incubated at 37°C / 48 h. Next, the number of colony-forming units per milliliter was counted and analyzed statistically (Tukey test, p ≤ 0.05). C. aurantium EO and NaOCl inhibited the growth of all microorganisms in multi-species biofilms. C. limonum EO promoted a 100% reduction of C. albicans and E. coli, and 49.3% of E. faecalis. CHX was less effective against C. albicans and E. coli, yielding a reduction of 68.8% and 86.7%, respectively. However, the reduction of E. faecalis using CHX (81.7%) was greater than that obtained using C. limonum EO. Both Citrus limonum and Citrus aurantium EOs are effective in controlling multi-species biofilms; the microbial reductions achieved by EOs were not only similar to those of NaOCl, but even higher than those achieved by CHX, in some cases. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09-30T18:18:33Z 2014-09-30T18:18:33Z 2014-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1806-83242013005000024 Brazilian Oral Research. Sociedade Brasileira de Pesquisa Odontológica - SBPqO, v. 28, n. 1, p. 22-27, 2014. 1806-8324 http://hdl.handle.net/11449/109663 10.1590/S1806-83242013005000024 S1806-83242014000100217 WOS:000328723700004 S1806-83242014000100217.pdf 0053567153623569 |
url |
http://dx.doi.org/10.1590/S1806-83242013005000024 http://hdl.handle.net/11449/109663 |
identifier_str_mv |
Brazilian Oral Research. Sociedade Brasileira de Pesquisa Odontológica - SBPqO, v. 28, n. 1, p. 22-27, 2014. 1806-8324 10.1590/S1806-83242013005000024 S1806-83242014000100217 WOS:000328723700004 S1806-83242014000100217.pdf 0053567153623569 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Oral Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
22-27 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129046376087552 |