Serotonin concentration, synthesis, cell origin, and targets in the rat caput epididymis during sexual maturation and variations associated with adult mating status: Morphological and biochemical studies
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.2164/jandrol.106.000653 http://repositorio.unifesp.br/handle/11600/29349 |
Resumo: | The caput epididymis of some mammals contains large quantities of serotonin whose origin, targets, and physiological variations have been poorly studied. We combined morphological and biochemical techniques to begin approaching these aspects of serotonin in the rat caput epididymis. Serotonin immunostaining was detected in mast, epithelial, and neuroendocrine cells. Epithelial cells displayed immunoreactivity to 5HT(1A), 5HT(2A), and 5HT(3) serotonin receptors. Endothelial and mast cells labeled positive for 5HT(1B) serotonin receptors and spermatozoa displayed 5HT2A and 5HT3 serotonin receptor immunoreactivity. Epithelial, endothelial, and mast cells stained positive for serotonin transporters. Only epithelial cells showed tryptophan hydroxylase immunoreactivity; this enzyme catalyzes the limiting step in the serotonin synthetic pathway. in addition, Western blot analyses of caput homogenates documented the presence of 2 protein bands (approximate to 51 kd and 48 kd) that were immunoreactive for tryptophan hydroxylase. Chromatographic analyses documented the presence of tryptophan hydroxylase in the caput, and showed that both its activity and serotonin availability increased with sexual maturation and decreased following p-chlorophenylalanine treatment, an inhibitor of tryptophan hydroxylase activity. Interestingly, serotonin concentration and tryptophan hydroxylase activity tended to be higher in breeding males than in those with no mating experience. We think that these results support the existence of a local serotoninergic system in the rat caput epididymis that might regulate some aspects of male reproductive function. |
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Serotonin concentration, synthesis, cell origin, and targets in the rat caput epididymis during sexual maturation and variations associated with adult mating status: Morphological and biochemical studies5-hydroxytryptaminetryptophan hydroxylaseneuroendocrine cellsmale reproductionserotonin receptorspermatozoaThe caput epididymis of some mammals contains large quantities of serotonin whose origin, targets, and physiological variations have been poorly studied. We combined morphological and biochemical techniques to begin approaching these aspects of serotonin in the rat caput epididymis. Serotonin immunostaining was detected in mast, epithelial, and neuroendocrine cells. Epithelial cells displayed immunoreactivity to 5HT(1A), 5HT(2A), and 5HT(3) serotonin receptors. Endothelial and mast cells labeled positive for 5HT(1B) serotonin receptors and spermatozoa displayed 5HT2A and 5HT3 serotonin receptor immunoreactivity. Epithelial, endothelial, and mast cells stained positive for serotonin transporters. Only epithelial cells showed tryptophan hydroxylase immunoreactivity; this enzyme catalyzes the limiting step in the serotonin synthetic pathway. in addition, Western blot analyses of caput homogenates documented the presence of 2 protein bands (approximate to 51 kd and 48 kd) that were immunoreactive for tryptophan hydroxylase. Chromatographic analyses documented the presence of tryptophan hydroxylase in the caput, and showed that both its activity and serotonin availability increased with sexual maturation and decreased following p-chlorophenylalanine treatment, an inhibitor of tryptophan hydroxylase activity. Interestingly, serotonin concentration and tryptophan hydroxylase activity tended to be higher in breeding males than in those with no mating experience. We think that these results support the existence of a local serotoninergic system in the rat caput epididymis that might regulate some aspects of male reproductive function.Natl Autonomous Univ Mexico, Biomed Res Inst, Dept Cell Biol & Physiol, Mexico City 04510, DF, MexicoUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, Sect Expt Endocrinol, São Paulo, BrazilNatl Autonomous Univ Mexico, Fac Sci, Dept Reprod Biol, Mexico City 04510, DF, MexicoXXI Century Natl Med Ctr, Unit Med Invest Neurol Dis, Mexico City, DF, MexicoUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, Sect Expt Endocrinol, São Paulo, BrazilWeb of ScienceAmer Soc Andrology, IncNatl Autonomous Univ MexicoUniversidade Federal de São Paulo (UNIFESP)XXI Century Natl Med CtrJimenez-Trejo, FranciscoTapia-Rodriguez, MiguelQueiroz, Daniel Barboza Cava [UNIFESP]Padilla, PatriciaAvellar, Maria Christina Werneck [UNIFESP]Manzano, Patricia RivasManjarrez-Gutierrez, GabrielGutierrez-Ospina, Gabriel2016-01-24T12:41:42Z2016-01-24T12:41:42Z2007-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion136-149http://dx.doi.org/10.2164/jandrol.106.000653Journal of Andrology. Lawrence: Amer Soc Andrology, Inc, v. 28, n. 1, p. 136-149, 2007.10.2164/jandrol.106.0006530196-3635http://repositorio.unifesp.br/handle/11600/29349WOS:000243141400018engJournal of Andrologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2022-06-02T09:02:18Zoai:repositorio.unifesp.br/:11600/29349Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652022-06-02T09:02:18Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Serotonin concentration, synthesis, cell origin, and targets in the rat caput epididymis during sexual maturation and variations associated with adult mating status: Morphological and biochemical studies |
title |
Serotonin concentration, synthesis, cell origin, and targets in the rat caput epididymis during sexual maturation and variations associated with adult mating status: Morphological and biochemical studies |
spellingShingle |
Serotonin concentration, synthesis, cell origin, and targets in the rat caput epididymis during sexual maturation and variations associated with adult mating status: Morphological and biochemical studies Jimenez-Trejo, Francisco 5-hydroxytryptamine tryptophan hydroxylase neuroendocrine cells male reproduction serotonin receptor spermatozoa |
title_short |
Serotonin concentration, synthesis, cell origin, and targets in the rat caput epididymis during sexual maturation and variations associated with adult mating status: Morphological and biochemical studies |
title_full |
Serotonin concentration, synthesis, cell origin, and targets in the rat caput epididymis during sexual maturation and variations associated with adult mating status: Morphological and biochemical studies |
title_fullStr |
Serotonin concentration, synthesis, cell origin, and targets in the rat caput epididymis during sexual maturation and variations associated with adult mating status: Morphological and biochemical studies |
title_full_unstemmed |
Serotonin concentration, synthesis, cell origin, and targets in the rat caput epididymis during sexual maturation and variations associated with adult mating status: Morphological and biochemical studies |
title_sort |
Serotonin concentration, synthesis, cell origin, and targets in the rat caput epididymis during sexual maturation and variations associated with adult mating status: Morphological and biochemical studies |
author |
Jimenez-Trejo, Francisco |
author_facet |
Jimenez-Trejo, Francisco Tapia-Rodriguez, Miguel Queiroz, Daniel Barboza Cava [UNIFESP] Padilla, Patricia Avellar, Maria Christina Werneck [UNIFESP] Manzano, Patricia Rivas Manjarrez-Gutierrez, Gabriel Gutierrez-Ospina, Gabriel |
author_role |
author |
author2 |
Tapia-Rodriguez, Miguel Queiroz, Daniel Barboza Cava [UNIFESP] Padilla, Patricia Avellar, Maria Christina Werneck [UNIFESP] Manzano, Patricia Rivas Manjarrez-Gutierrez, Gabriel Gutierrez-Ospina, Gabriel |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Natl Autonomous Univ Mexico Universidade Federal de São Paulo (UNIFESP) XXI Century Natl Med Ctr |
dc.contributor.author.fl_str_mv |
Jimenez-Trejo, Francisco Tapia-Rodriguez, Miguel Queiroz, Daniel Barboza Cava [UNIFESP] Padilla, Patricia Avellar, Maria Christina Werneck [UNIFESP] Manzano, Patricia Rivas Manjarrez-Gutierrez, Gabriel Gutierrez-Ospina, Gabriel |
dc.subject.por.fl_str_mv |
5-hydroxytryptamine tryptophan hydroxylase neuroendocrine cells male reproduction serotonin receptor spermatozoa |
topic |
5-hydroxytryptamine tryptophan hydroxylase neuroendocrine cells male reproduction serotonin receptor spermatozoa |
description |
The caput epididymis of some mammals contains large quantities of serotonin whose origin, targets, and physiological variations have been poorly studied. We combined morphological and biochemical techniques to begin approaching these aspects of serotonin in the rat caput epididymis. Serotonin immunostaining was detected in mast, epithelial, and neuroendocrine cells. Epithelial cells displayed immunoreactivity to 5HT(1A), 5HT(2A), and 5HT(3) serotonin receptors. Endothelial and mast cells labeled positive for 5HT(1B) serotonin receptors and spermatozoa displayed 5HT2A and 5HT3 serotonin receptor immunoreactivity. Epithelial, endothelial, and mast cells stained positive for serotonin transporters. Only epithelial cells showed tryptophan hydroxylase immunoreactivity; this enzyme catalyzes the limiting step in the serotonin synthetic pathway. in addition, Western blot analyses of caput homogenates documented the presence of 2 protein bands (approximate to 51 kd and 48 kd) that were immunoreactive for tryptophan hydroxylase. Chromatographic analyses documented the presence of tryptophan hydroxylase in the caput, and showed that both its activity and serotonin availability increased with sexual maturation and decreased following p-chlorophenylalanine treatment, an inhibitor of tryptophan hydroxylase activity. Interestingly, serotonin concentration and tryptophan hydroxylase activity tended to be higher in breeding males than in those with no mating experience. We think that these results support the existence of a local serotoninergic system in the rat caput epididymis that might regulate some aspects of male reproductive function. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-01-01 2016-01-24T12:41:42Z 2016-01-24T12:41:42Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.2164/jandrol.106.000653 Journal of Andrology. Lawrence: Amer Soc Andrology, Inc, v. 28, n. 1, p. 136-149, 2007. 10.2164/jandrol.106.000653 0196-3635 http://repositorio.unifesp.br/handle/11600/29349 WOS:000243141400018 |
url |
http://dx.doi.org/10.2164/jandrol.106.000653 http://repositorio.unifesp.br/handle/11600/29349 |
identifier_str_mv |
Journal of Andrology. Lawrence: Amer Soc Andrology, Inc, v. 28, n. 1, p. 136-149, 2007. 10.2164/jandrol.106.000653 0196-3635 WOS:000243141400018 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Andrology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
136-149 |
dc.publisher.none.fl_str_mv |
Amer Soc Andrology, Inc |
publisher.none.fl_str_mv |
Amer Soc Andrology, Inc |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268282279034880 |