Humoral autoimmune response heterogeneity in the spectrum of primary biliary cirrhosis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1007/s12072-012-9413-0 http://repositorio.unifesp.br/handle/11600/36358 |
Resumo: | To compare autoantibody features in patients with primary biliary cirrhosis (PBC) and individuals presenting antimitochondria antibodies (AMAs) but no clinical or biochemical evidence of disease.A total of 212 AMA-positive serum samples were classified into four groups: PBC (definite PBC, n = 93); PBC/autoimmune disease (AID; PBC plus other AID, n = 37); biochemically normal (BN) individuals (n = 61); and BN/AID (BN plus other AID, n = 21). Samples were tested by indirect immunofluorescence (IIF) on rat kidney (IIF-AMA) and ELISA [antibodies to pyruvate dehydrogenase E2-complex (PDC-E2), gp-210, Sp-100, and CENP-A/B]. AMA isotype was determined by IIF-AMA. Affinity of anti-PDC-E2 IgG was determined by 8 M urea-modified ELISA.High-titer IIF-AMA was more frequent in PBC and PBC/AID (57 and 70 %) than in BN and BN/AID samples (23 and 19 %) (p < 0.001). Triple isotype IIF-AMA (IgA/IgM/IgG) was more frequent in PBC and PBC/AID samples (35 and 43 %) than in BN sample (18 %; p = 0.008; p = 0.013, respectively). Anti-PDC-E2 levels were higher in PBC (mean 3.82; 95 % CI 3.36-4.29) and PBC/AID samples (3.89; 3.15-4.63) than in BN (2.43; 1.92-2.94) and BN/AID samples (2.52; 1.54-3.50) (p < 0.001). Anti-PDC-E2 avidity was higher in PBC (mean 64.5 %; 95 % CI 57.5-71.5 %) and PBC/AID samples (66.1 %; 54.4-77.8 %) than in BN samples (39.2 %; 30.9-37.5 %) (p < 0.001). PBC and PBC/AID recognized more cell domains (mitochondria, nuclear envelope, PML/sp-100 bodies, centromere) than BN (p = 0.008) and BN/AID samples (p = 0.002). Three variables were independently associated with established PBC: high-avidity anti-PDC-E2 (OR 4.121; 95 % CI 2.118-8.019); high-titer IIF-AMA (OR 4.890; 2.319-10.314); antibodies to three or more antigenic cell domains (OR 9.414; 1.924-46.060).The autoantibody profile was quantitatively and qualitatively more robust in definite PBC as compared with AMA-positive biochemically normal individuals. |
id |
UFSP_b6a73f49be7249c976b64d7e6dc95a05 |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br/:11600/36358 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Humoral autoimmune response heterogeneity in the spectrum of primary biliary cirrhosisAutoimmunityAntibody affinityAutoimmune liver diseasesAutoantibodiesTo compare autoantibody features in patients with primary biliary cirrhosis (PBC) and individuals presenting antimitochondria antibodies (AMAs) but no clinical or biochemical evidence of disease.A total of 212 AMA-positive serum samples were classified into four groups: PBC (definite PBC, n = 93); PBC/autoimmune disease (AID; PBC plus other AID, n = 37); biochemically normal (BN) individuals (n = 61); and BN/AID (BN plus other AID, n = 21). Samples were tested by indirect immunofluorescence (IIF) on rat kidney (IIF-AMA) and ELISA [antibodies to pyruvate dehydrogenase E2-complex (PDC-E2), gp-210, Sp-100, and CENP-A/B]. AMA isotype was determined by IIF-AMA. Affinity of anti-PDC-E2 IgG was determined by 8 M urea-modified ELISA.High-titer IIF-AMA was more frequent in PBC and PBC/AID (57 and 70 %) than in BN and BN/AID samples (23 and 19 %) (p < 0.001). Triple isotype IIF-AMA (IgA/IgM/IgG) was more frequent in PBC and PBC/AID samples (35 and 43 %) than in BN sample (18 %; p = 0.008; p = 0.013, respectively). Anti-PDC-E2 levels were higher in PBC (mean 3.82; 95 % CI 3.36-4.29) and PBC/AID samples (3.89; 3.15-4.63) than in BN (2.43; 1.92-2.94) and BN/AID samples (2.52; 1.54-3.50) (p < 0.001). Anti-PDC-E2 avidity was higher in PBC (mean 64.5 %; 95 % CI 57.5-71.5 %) and PBC/AID samples (66.1 %; 54.4-77.8 %) than in BN samples (39.2 %; 30.9-37.5 %) (p < 0.001). PBC and PBC/AID recognized more cell domains (mitochondria, nuclear envelope, PML/sp-100 bodies, centromere) than BN (p = 0.008) and BN/AID samples (p = 0.002). Three variables were independently associated with established PBC: high-avidity anti-PDC-E2 (OR 4.121; 95 % CI 2.118-8.019); high-titer IIF-AMA (OR 4.890; 2.319-10.314); antibodies to three or more antigenic cell domains (OR 9.414; 1.924-46.060).The autoantibody profile was quantitatively and qualitatively more robust in definite PBC as compared with AMA-positive biochemically normal individuals.Universidade Federal de São Paulo, UNIFESP, Div Rheumatol, BR-04023900 São Paulo, BrazilFleury Med & Hlth Labs, Div Res & Dev, São Paulo, BrazilUniv São Paulo, Sch Med, Dept Gastroenterol, São Paulo, BrazilUniv São Paulo, Inst Trop, Lab Med Invest LIM 06, São Paulo, BrazilUniversidade Federal de São Paulo, UNIFESP, Hosp Servidor Publ Estadual Francisco Morato Oliv, São Paulo, BrazilUniversidade Federal de São Paulo, UNIFESP, Dept Stat, São Paulo, BrazilUniversidade Federal de São Paulo, UNIFESP, Div Rheumatol, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, UNIFESP, Hosp Servidor Publ Estadual Francisco Morato Oliv, São Paulo, BrazilUniversidade Federal de São Paulo, UNIFESP, Dept Stat, São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Research and Development Department of Fleury Medicine and HealthConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FAPESP: 2009/51887-0CNPq: 476356/2008-3SpringerUniversidade Federal de São Paulo (UNIFESP)Fleury Med & Hlth LabsUniversidade de São Paulo (USP)Dellavance, Alessandra [UNIFESP]Cancado, Eduardo Luiz RachidAbrantes-Lemos, Clarice PiresHarriz, Michelle [UNIFESP]Marvulle, Valdecir [UNIFESP]Andrade, Luiz Eduardo Coelho [UNIFESP]2016-01-24T14:31:48Z2016-01-24T14:31:48Z2013-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion775-784application/pdfhttp://dx.doi.org/10.1007/s12072-012-9413-0Hepatology International. New York: Springer, v. 7, n. 2, p. 775-784, 2013.10.1007/s12072-012-9413-0WOS000321127600056.pdf1936-0533http://repositorio.unifesp.br/handle/11600/36358WOS:000321127600056engHepatology Internationalinfo:eu-repo/semantics/openAccesshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-07T18:04:02Zoai:repositorio.unifesp.br/:11600/36358Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-07T18:04:02Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Humoral autoimmune response heterogeneity in the spectrum of primary biliary cirrhosis |
title |
Humoral autoimmune response heterogeneity in the spectrum of primary biliary cirrhosis |
spellingShingle |
Humoral autoimmune response heterogeneity in the spectrum of primary biliary cirrhosis Dellavance, Alessandra [UNIFESP] Autoimmunity Antibody affinity Autoimmune liver diseases Autoantibodies |
title_short |
Humoral autoimmune response heterogeneity in the spectrum of primary biliary cirrhosis |
title_full |
Humoral autoimmune response heterogeneity in the spectrum of primary biliary cirrhosis |
title_fullStr |
Humoral autoimmune response heterogeneity in the spectrum of primary biliary cirrhosis |
title_full_unstemmed |
Humoral autoimmune response heterogeneity in the spectrum of primary biliary cirrhosis |
title_sort |
Humoral autoimmune response heterogeneity in the spectrum of primary biliary cirrhosis |
author |
Dellavance, Alessandra [UNIFESP] |
author_facet |
Dellavance, Alessandra [UNIFESP] Cancado, Eduardo Luiz Rachid Abrantes-Lemos, Clarice Pires Harriz, Michelle [UNIFESP] Marvulle, Valdecir [UNIFESP] Andrade, Luiz Eduardo Coelho [UNIFESP] |
author_role |
author |
author2 |
Cancado, Eduardo Luiz Rachid Abrantes-Lemos, Clarice Pires Harriz, Michelle [UNIFESP] Marvulle, Valdecir [UNIFESP] Andrade, Luiz Eduardo Coelho [UNIFESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Fleury Med & Hlth Labs Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Dellavance, Alessandra [UNIFESP] Cancado, Eduardo Luiz Rachid Abrantes-Lemos, Clarice Pires Harriz, Michelle [UNIFESP] Marvulle, Valdecir [UNIFESP] Andrade, Luiz Eduardo Coelho [UNIFESP] |
dc.subject.por.fl_str_mv |
Autoimmunity Antibody affinity Autoimmune liver diseases Autoantibodies |
topic |
Autoimmunity Antibody affinity Autoimmune liver diseases Autoantibodies |
description |
To compare autoantibody features in patients with primary biliary cirrhosis (PBC) and individuals presenting antimitochondria antibodies (AMAs) but no clinical or biochemical evidence of disease.A total of 212 AMA-positive serum samples were classified into four groups: PBC (definite PBC, n = 93); PBC/autoimmune disease (AID; PBC plus other AID, n = 37); biochemically normal (BN) individuals (n = 61); and BN/AID (BN plus other AID, n = 21). Samples were tested by indirect immunofluorescence (IIF) on rat kidney (IIF-AMA) and ELISA [antibodies to pyruvate dehydrogenase E2-complex (PDC-E2), gp-210, Sp-100, and CENP-A/B]. AMA isotype was determined by IIF-AMA. Affinity of anti-PDC-E2 IgG was determined by 8 M urea-modified ELISA.High-titer IIF-AMA was more frequent in PBC and PBC/AID (57 and 70 %) than in BN and BN/AID samples (23 and 19 %) (p < 0.001). Triple isotype IIF-AMA (IgA/IgM/IgG) was more frequent in PBC and PBC/AID samples (35 and 43 %) than in BN sample (18 %; p = 0.008; p = 0.013, respectively). Anti-PDC-E2 levels were higher in PBC (mean 3.82; 95 % CI 3.36-4.29) and PBC/AID samples (3.89; 3.15-4.63) than in BN (2.43; 1.92-2.94) and BN/AID samples (2.52; 1.54-3.50) (p < 0.001). Anti-PDC-E2 avidity was higher in PBC (mean 64.5 %; 95 % CI 57.5-71.5 %) and PBC/AID samples (66.1 %; 54.4-77.8 %) than in BN samples (39.2 %; 30.9-37.5 %) (p < 0.001). PBC and PBC/AID recognized more cell domains (mitochondria, nuclear envelope, PML/sp-100 bodies, centromere) than BN (p = 0.008) and BN/AID samples (p = 0.002). Three variables were independently associated with established PBC: high-avidity anti-PDC-E2 (OR 4.121; 95 % CI 2.118-8.019); high-titer IIF-AMA (OR 4.890; 2.319-10.314); antibodies to three or more antigenic cell domains (OR 9.414; 1.924-46.060).The autoantibody profile was quantitatively and qualitatively more robust in definite PBC as compared with AMA-positive biochemically normal individuals. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-06-01 2016-01-24T14:31:48Z 2016-01-24T14:31:48Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s12072-012-9413-0 Hepatology International. New York: Springer, v. 7, n. 2, p. 775-784, 2013. 10.1007/s12072-012-9413-0 WOS000321127600056.pdf 1936-0533 http://repositorio.unifesp.br/handle/11600/36358 WOS:000321127600056 |
url |
http://dx.doi.org/10.1007/s12072-012-9413-0 http://repositorio.unifesp.br/handle/11600/36358 |
identifier_str_mv |
Hepatology International. New York: Springer, v. 7, n. 2, p. 775-784, 2013. 10.1007/s12072-012-9413-0 WOS000321127600056.pdf 1936-0533 WOS:000321127600056 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Hepatology International |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0 |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0 |
dc.format.none.fl_str_mv |
775-784 application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268432088039424 |