Humoral autoimmune response heterogeneity in the spectrum of primary biliary cirrhosis

Detalhes bibliográficos
Autor(a) principal: Dellavance, Alessandra [UNIFESP]
Data de Publicação: 2013
Outros Autores: Cancado, Eduardo Luiz Rachid, Abrantes-Lemos, Clarice Pires, Harriz, Michelle [UNIFESP], Marvulle, Valdecir [UNIFESP], Andrade, Luiz Eduardo Coelho [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1007/s12072-012-9413-0
http://repositorio.unifesp.br/handle/11600/36358
Resumo: To compare autoantibody features in patients with primary biliary cirrhosis (PBC) and individuals presenting antimitochondria antibodies (AMAs) but no clinical or biochemical evidence of disease.A total of 212 AMA-positive serum samples were classified into four groups: PBC (definite PBC, n = 93); PBC/autoimmune disease (AID; PBC plus other AID, n = 37); biochemically normal (BN) individuals (n = 61); and BN/AID (BN plus other AID, n = 21). Samples were tested by indirect immunofluorescence (IIF) on rat kidney (IIF-AMA) and ELISA [antibodies to pyruvate dehydrogenase E2-complex (PDC-E2), gp-210, Sp-100, and CENP-A/B]. AMA isotype was determined by IIF-AMA. Affinity of anti-PDC-E2 IgG was determined by 8 M urea-modified ELISA.High-titer IIF-AMA was more frequent in PBC and PBC/AID (57 and 70 %) than in BN and BN/AID samples (23 and 19 %) (p < 0.001). Triple isotype IIF-AMA (IgA/IgM/IgG) was more frequent in PBC and PBC/AID samples (35 and 43 %) than in BN sample (18 %; p = 0.008; p = 0.013, respectively). Anti-PDC-E2 levels were higher in PBC (mean 3.82; 95 % CI 3.36-4.29) and PBC/AID samples (3.89; 3.15-4.63) than in BN (2.43; 1.92-2.94) and BN/AID samples (2.52; 1.54-3.50) (p < 0.001). Anti-PDC-E2 avidity was higher in PBC (mean 64.5 %; 95 % CI 57.5-71.5 %) and PBC/AID samples (66.1 %; 54.4-77.8 %) than in BN samples (39.2 %; 30.9-37.5 %) (p < 0.001). PBC and PBC/AID recognized more cell domains (mitochondria, nuclear envelope, PML/sp-100 bodies, centromere) than BN (p = 0.008) and BN/AID samples (p = 0.002). Three variables were independently associated with established PBC: high-avidity anti-PDC-E2 (OR 4.121; 95 % CI 2.118-8.019); high-titer IIF-AMA (OR 4.890; 2.319-10.314); antibodies to three or more antigenic cell domains (OR 9.414; 1.924-46.060).The autoantibody profile was quantitatively and qualitatively more robust in definite PBC as compared with AMA-positive biochemically normal individuals.
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spelling Humoral autoimmune response heterogeneity in the spectrum of primary biliary cirrhosisAutoimmunityAntibody affinityAutoimmune liver diseasesAutoantibodiesTo compare autoantibody features in patients with primary biliary cirrhosis (PBC) and individuals presenting antimitochondria antibodies (AMAs) but no clinical or biochemical evidence of disease.A total of 212 AMA-positive serum samples were classified into four groups: PBC (definite PBC, n = 93); PBC/autoimmune disease (AID; PBC plus other AID, n = 37); biochemically normal (BN) individuals (n = 61); and BN/AID (BN plus other AID, n = 21). Samples were tested by indirect immunofluorescence (IIF) on rat kidney (IIF-AMA) and ELISA [antibodies to pyruvate dehydrogenase E2-complex (PDC-E2), gp-210, Sp-100, and CENP-A/B]. AMA isotype was determined by IIF-AMA. Affinity of anti-PDC-E2 IgG was determined by 8 M urea-modified ELISA.High-titer IIF-AMA was more frequent in PBC and PBC/AID (57 and 70 %) than in BN and BN/AID samples (23 and 19 %) (p < 0.001). Triple isotype IIF-AMA (IgA/IgM/IgG) was more frequent in PBC and PBC/AID samples (35 and 43 %) than in BN sample (18 %; p = 0.008; p = 0.013, respectively). Anti-PDC-E2 levels were higher in PBC (mean 3.82; 95 % CI 3.36-4.29) and PBC/AID samples (3.89; 3.15-4.63) than in BN (2.43; 1.92-2.94) and BN/AID samples (2.52; 1.54-3.50) (p < 0.001). Anti-PDC-E2 avidity was higher in PBC (mean 64.5 %; 95 % CI 57.5-71.5 %) and PBC/AID samples (66.1 %; 54.4-77.8 %) than in BN samples (39.2 %; 30.9-37.5 %) (p < 0.001). PBC and PBC/AID recognized more cell domains (mitochondria, nuclear envelope, PML/sp-100 bodies, centromere) than BN (p = 0.008) and BN/AID samples (p = 0.002). Three variables were independently associated with established PBC: high-avidity anti-PDC-E2 (OR 4.121; 95 % CI 2.118-8.019); high-titer IIF-AMA (OR 4.890; 2.319-10.314); antibodies to three or more antigenic cell domains (OR 9.414; 1.924-46.060).The autoantibody profile was quantitatively and qualitatively more robust in definite PBC as compared with AMA-positive biochemically normal individuals.Universidade Federal de São Paulo, UNIFESP, Div Rheumatol, BR-04023900 São Paulo, BrazilFleury Med & Hlth Labs, Div Res & Dev, São Paulo, BrazilUniv São Paulo, Sch Med, Dept Gastroenterol, São Paulo, BrazilUniv São Paulo, Inst Trop, Lab Med Invest LIM 06, São Paulo, BrazilUniversidade Federal de São Paulo, UNIFESP, Hosp Servidor Publ Estadual Francisco Morato Oliv, São Paulo, BrazilUniversidade Federal de São Paulo, UNIFESP, Dept Stat, São Paulo, BrazilUniversidade Federal de São Paulo, UNIFESP, Div Rheumatol, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, UNIFESP, Hosp Servidor Publ Estadual Francisco Morato Oliv, São Paulo, BrazilUniversidade Federal de São Paulo, UNIFESP, Dept Stat, São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Research and Development Department of Fleury Medicine and HealthConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FAPESP: 2009/51887-0CNPq: 476356/2008-3SpringerUniversidade Federal de São Paulo (UNIFESP)Fleury Med & Hlth LabsUniversidade de São Paulo (USP)Dellavance, Alessandra [UNIFESP]Cancado, Eduardo Luiz RachidAbrantes-Lemos, Clarice PiresHarriz, Michelle [UNIFESP]Marvulle, Valdecir [UNIFESP]Andrade, Luiz Eduardo Coelho [UNIFESP]2016-01-24T14:31:48Z2016-01-24T14:31:48Z2013-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion775-784application/pdfhttp://dx.doi.org/10.1007/s12072-012-9413-0Hepatology International. New York: Springer, v. 7, n. 2, p. 775-784, 2013.10.1007/s12072-012-9413-0WOS000321127600056.pdf1936-0533http://repositorio.unifesp.br/handle/11600/36358WOS:000321127600056engHepatology Internationalinfo:eu-repo/semantics/openAccesshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-07T18:04:02Zoai:repositorio.unifesp.br/:11600/36358Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-07T18:04:02Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Humoral autoimmune response heterogeneity in the spectrum of primary biliary cirrhosis
title Humoral autoimmune response heterogeneity in the spectrum of primary biliary cirrhosis
spellingShingle Humoral autoimmune response heterogeneity in the spectrum of primary biliary cirrhosis
Dellavance, Alessandra [UNIFESP]
Autoimmunity
Antibody affinity
Autoimmune liver diseases
Autoantibodies
title_short Humoral autoimmune response heterogeneity in the spectrum of primary biliary cirrhosis
title_full Humoral autoimmune response heterogeneity in the spectrum of primary biliary cirrhosis
title_fullStr Humoral autoimmune response heterogeneity in the spectrum of primary biliary cirrhosis
title_full_unstemmed Humoral autoimmune response heterogeneity in the spectrum of primary biliary cirrhosis
title_sort Humoral autoimmune response heterogeneity in the spectrum of primary biliary cirrhosis
author Dellavance, Alessandra [UNIFESP]
author_facet Dellavance, Alessandra [UNIFESP]
Cancado, Eduardo Luiz Rachid
Abrantes-Lemos, Clarice Pires
Harriz, Michelle [UNIFESP]
Marvulle, Valdecir [UNIFESP]
Andrade, Luiz Eduardo Coelho [UNIFESP]
author_role author
author2 Cancado, Eduardo Luiz Rachid
Abrantes-Lemos, Clarice Pires
Harriz, Michelle [UNIFESP]
Marvulle, Valdecir [UNIFESP]
Andrade, Luiz Eduardo Coelho [UNIFESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Fleury Med & Hlth Labs
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Dellavance, Alessandra [UNIFESP]
Cancado, Eduardo Luiz Rachid
Abrantes-Lemos, Clarice Pires
Harriz, Michelle [UNIFESP]
Marvulle, Valdecir [UNIFESP]
Andrade, Luiz Eduardo Coelho [UNIFESP]
dc.subject.por.fl_str_mv Autoimmunity
Antibody affinity
Autoimmune liver diseases
Autoantibodies
topic Autoimmunity
Antibody affinity
Autoimmune liver diseases
Autoantibodies
description To compare autoantibody features in patients with primary biliary cirrhosis (PBC) and individuals presenting antimitochondria antibodies (AMAs) but no clinical or biochemical evidence of disease.A total of 212 AMA-positive serum samples were classified into four groups: PBC (definite PBC, n = 93); PBC/autoimmune disease (AID; PBC plus other AID, n = 37); biochemically normal (BN) individuals (n = 61); and BN/AID (BN plus other AID, n = 21). Samples were tested by indirect immunofluorescence (IIF) on rat kidney (IIF-AMA) and ELISA [antibodies to pyruvate dehydrogenase E2-complex (PDC-E2), gp-210, Sp-100, and CENP-A/B]. AMA isotype was determined by IIF-AMA. Affinity of anti-PDC-E2 IgG was determined by 8 M urea-modified ELISA.High-titer IIF-AMA was more frequent in PBC and PBC/AID (57 and 70 %) than in BN and BN/AID samples (23 and 19 %) (p < 0.001). Triple isotype IIF-AMA (IgA/IgM/IgG) was more frequent in PBC and PBC/AID samples (35 and 43 %) than in BN sample (18 %; p = 0.008; p = 0.013, respectively). Anti-PDC-E2 levels were higher in PBC (mean 3.82; 95 % CI 3.36-4.29) and PBC/AID samples (3.89; 3.15-4.63) than in BN (2.43; 1.92-2.94) and BN/AID samples (2.52; 1.54-3.50) (p < 0.001). Anti-PDC-E2 avidity was higher in PBC (mean 64.5 %; 95 % CI 57.5-71.5 %) and PBC/AID samples (66.1 %; 54.4-77.8 %) than in BN samples (39.2 %; 30.9-37.5 %) (p < 0.001). PBC and PBC/AID recognized more cell domains (mitochondria, nuclear envelope, PML/sp-100 bodies, centromere) than BN (p = 0.008) and BN/AID samples (p = 0.002). Three variables were independently associated with established PBC: high-avidity anti-PDC-E2 (OR 4.121; 95 % CI 2.118-8.019); high-titer IIF-AMA (OR 4.890; 2.319-10.314); antibodies to three or more antigenic cell domains (OR 9.414; 1.924-46.060).The autoantibody profile was quantitatively and qualitatively more robust in definite PBC as compared with AMA-positive biochemically normal individuals.
publishDate 2013
dc.date.none.fl_str_mv 2013-06-01
2016-01-24T14:31:48Z
2016-01-24T14:31:48Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s12072-012-9413-0
Hepatology International. New York: Springer, v. 7, n. 2, p. 775-784, 2013.
10.1007/s12072-012-9413-0
WOS000321127600056.pdf
1936-0533
http://repositorio.unifesp.br/handle/11600/36358
WOS:000321127600056
url http://dx.doi.org/10.1007/s12072-012-9413-0
http://repositorio.unifesp.br/handle/11600/36358
identifier_str_mv Hepatology International. New York: Springer, v. 7, n. 2, p. 775-784, 2013.
10.1007/s12072-012-9413-0
WOS000321127600056.pdf
1936-0533
WOS:000321127600056
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Hepatology International
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.format.none.fl_str_mv 775-784
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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