Rapid tests for the detection of the Mycobacterium abscessus subsp. bolletii strain responsible for an epidemic of surgical-site infections in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/7448 http://dx.doi.org/10.1590/S0074-02762012000800002 |
Resumo: | A single strain of Mycobacterium abscessus subsp. bolletii, characterised by a particular rpoB sequevar and two highly related pulsed field gel electrophoresis patterns has been responsible for a nationwide outbreak of surgical infections in Brazil since 2004. In this study, we developed molecular tests based on polymerase chain reaction restriction-enzyme analysis (PRA) and sequencing for the rapid identification of this strain. Sequences of 15 DNA regions conserved in mycobacteria were retrieved from GenBank or sequenced and analysed in silico. Single nucleotide polymorphisms specific to the epidemic strain and located in enzyme recognition sites were detected in rpoB, the 3' region of the 16S rDNA and gyrB. The three tests that were developed, i.e., PRA-rpoB, PRA-16S and gyrB sequence analysis, showed 100%, 100% and 92.31% sensitivity and 93.06%, 90.28% and 100% specificity, respectively, for the discrimination of the surgical strain from other M. abscessus subsp. bolletii isolates, including 116 isolates from 95 patients, one environmental isolate and two type strains. The results of the three tests were stable, as shown by results obtained for different isolates from the same patient. In conclusion, due to the clinical and epidemiological importance of this strain, these tests could be implemented in reference laboratories for the rapid preliminary diagnosis and epidemiological surveillance of this epidemic strain. |
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Matsumoto, Cristianne Kayoko [UNIFESP]Chimara, EricaRamos, Jesus PaisCampos, Carlos Eduardo DiasCaldas, Paulo Cesar de SouzaLima, Karla Valeria BatistaLopes, Maria LuizaDuarte, Rafael SilvaLeao, Sylvia Cardoso [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Instituto Adolfo LutzEscola Nacional de Saúde Pública-Fiocruz Centro de Referência Professor Hélio FragaInstituto Evandro ChagasUniversidade Federal do Rio de Janeiro Instituto de Microbiologia Professor Paulo de Góes2015-06-14T13:45:05Z2015-06-14T13:45:05Z2012-12-01Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 107, n. 8, p. 969-977, 2012.0074-0276http://repositorio.unifesp.br/handle/11600/7448http://dx.doi.org/10.1590/S0074-02762012000800002S0074-02762012000800002.pdfS0074-0276201200080000210.1590/S0074-02762012000800002WOS:000328105100002A single strain of Mycobacterium abscessus subsp. bolletii, characterised by a particular rpoB sequevar and two highly related pulsed field gel electrophoresis patterns has been responsible for a nationwide outbreak of surgical infections in Brazil since 2004. In this study, we developed molecular tests based on polymerase chain reaction restriction-enzyme analysis (PRA) and sequencing for the rapid identification of this strain. Sequences of 15 DNA regions conserved in mycobacteria were retrieved from GenBank or sequenced and analysed in silico. Single nucleotide polymorphisms specific to the epidemic strain and located in enzyme recognition sites were detected in rpoB, the 3' region of the 16S rDNA and gyrB. The three tests that were developed, i.e., PRA-rpoB, PRA-16S and gyrB sequence analysis, showed 100%, 100% and 92.31% sensitivity and 93.06%, 90.28% and 100% specificity, respectively, for the discrimination of the surgical strain from other M. abscessus subsp. bolletii isolates, including 116 isolates from 95 patients, one environmental isolate and two type strains. The results of the three tests were stable, as shown by results obtained for different isolates from the same patient. In conclusion, due to the clinical and epidemiological importance of this strain, these tests could be implemented in reference laboratories for the rapid preliminary diagnosis and epidemiological surveillance of this epidemic strain.Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Microbiologia, Imunologia e ParasitologiaInstituto Adolfo LutzEscola Nacional de Saúde Pública-Fiocruz Centro de Referência Professor Hélio FragaInstituto Evandro ChagasUniversidade Federal do Rio de Janeiro Instituto de Microbiologia Professor Paulo de GóesUNIFESP, EPM, Depto. de Microbiologia, Imunologia e ParasitologiaSciELO969-977engInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo CruzMycobacterium abscessusepidemicrapid testrpoBepidemiological surveillanceRapid tests for the detection of the Mycobacterium abscessus subsp. bolletii strain responsible for an epidemic of surgical-site infections in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS0074-02762012000800002.pdfapplication/pdf1040073${dspace.ui.url}/bitstream/11600/7448/1/S0074-02762012000800002.pdf77cdc651973a8b7991a1c79ac66add04MD51open accessTEXTS0074-02762012000800002.pdf.txtS0074-02762012000800002.pdf.txtExtracted texttext/plain42849${dspace.ui.url}/bitstream/11600/7448/2/S0074-02762012000800002.pdf.txt9b9d108365e9f9a87a38ca717d2a58fcMD52open access11600/74482022-02-08 11:52:12.537open accessoai:repositorio.unifesp.br:11600/7448Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:23:21.185257Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Rapid tests for the detection of the Mycobacterium abscessus subsp. bolletii strain responsible for an epidemic of surgical-site infections in Brazil |
title |
Rapid tests for the detection of the Mycobacterium abscessus subsp. bolletii strain responsible for an epidemic of surgical-site infections in Brazil |
spellingShingle |
Rapid tests for the detection of the Mycobacterium abscessus subsp. bolletii strain responsible for an epidemic of surgical-site infections in Brazil Matsumoto, Cristianne Kayoko [UNIFESP] Mycobacterium abscessus epidemic rapid test rpoB epidemiological surveillance |
title_short |
Rapid tests for the detection of the Mycobacterium abscessus subsp. bolletii strain responsible for an epidemic of surgical-site infections in Brazil |
title_full |
Rapid tests for the detection of the Mycobacterium abscessus subsp. bolletii strain responsible for an epidemic of surgical-site infections in Brazil |
title_fullStr |
Rapid tests for the detection of the Mycobacterium abscessus subsp. bolletii strain responsible for an epidemic of surgical-site infections in Brazil |
title_full_unstemmed |
Rapid tests for the detection of the Mycobacterium abscessus subsp. bolletii strain responsible for an epidemic of surgical-site infections in Brazil |
title_sort |
Rapid tests for the detection of the Mycobacterium abscessus subsp. bolletii strain responsible for an epidemic of surgical-site infections in Brazil |
author |
Matsumoto, Cristianne Kayoko [UNIFESP] |
author_facet |
Matsumoto, Cristianne Kayoko [UNIFESP] Chimara, Erica Ramos, Jesus Pais Campos, Carlos Eduardo Dias Caldas, Paulo Cesar de Souza Lima, Karla Valeria Batista Lopes, Maria Luiza Duarte, Rafael Silva Leao, Sylvia Cardoso [UNIFESP] |
author_role |
author |
author2 |
Chimara, Erica Ramos, Jesus Pais Campos, Carlos Eduardo Dias Caldas, Paulo Cesar de Souza Lima, Karla Valeria Batista Lopes, Maria Luiza Duarte, Rafael Silva Leao, Sylvia Cardoso [UNIFESP] |
author2_role |
author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Instituto Adolfo Lutz Escola Nacional de Saúde Pública-Fiocruz Centro de Referência Professor Hélio Fraga Instituto Evandro Chagas Universidade Federal do Rio de Janeiro Instituto de Microbiologia Professor Paulo de Góes |
dc.contributor.author.fl_str_mv |
Matsumoto, Cristianne Kayoko [UNIFESP] Chimara, Erica Ramos, Jesus Pais Campos, Carlos Eduardo Dias Caldas, Paulo Cesar de Souza Lima, Karla Valeria Batista Lopes, Maria Luiza Duarte, Rafael Silva Leao, Sylvia Cardoso [UNIFESP] |
dc.subject.eng.fl_str_mv |
Mycobacterium abscessus epidemic rapid test rpoB epidemiological surveillance |
topic |
Mycobacterium abscessus epidemic rapid test rpoB epidemiological surveillance |
description |
A single strain of Mycobacterium abscessus subsp. bolletii, characterised by a particular rpoB sequevar and two highly related pulsed field gel electrophoresis patterns has been responsible for a nationwide outbreak of surgical infections in Brazil since 2004. In this study, we developed molecular tests based on polymerase chain reaction restriction-enzyme analysis (PRA) and sequencing for the rapid identification of this strain. Sequences of 15 DNA regions conserved in mycobacteria were retrieved from GenBank or sequenced and analysed in silico. Single nucleotide polymorphisms specific to the epidemic strain and located in enzyme recognition sites were detected in rpoB, the 3' region of the 16S rDNA and gyrB. The three tests that were developed, i.e., PRA-rpoB, PRA-16S and gyrB sequence analysis, showed 100%, 100% and 92.31% sensitivity and 93.06%, 90.28% and 100% specificity, respectively, for the discrimination of the surgical strain from other M. abscessus subsp. bolletii isolates, including 116 isolates from 95 patients, one environmental isolate and two type strains. The results of the three tests were stable, as shown by results obtained for different isolates from the same patient. In conclusion, due to the clinical and epidemiological importance of this strain, these tests could be implemented in reference laboratories for the rapid preliminary diagnosis and epidemiological surveillance of this epidemic strain. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-12-01 |
dc.date.accessioned.fl_str_mv |
2015-06-14T13:45:05Z |
dc.date.available.fl_str_mv |
2015-06-14T13:45:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 107, n. 8, p. 969-977, 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/7448 http://dx.doi.org/10.1590/S0074-02762012000800002 |
dc.identifier.issn.none.fl_str_mv |
0074-0276 |
dc.identifier.file.none.fl_str_mv |
S0074-02762012000800002.pdf |
dc.identifier.scielo.none.fl_str_mv |
S0074-02762012000800002 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S0074-02762012000800002 |
dc.identifier.wos.none.fl_str_mv |
WOS:000328105100002 |
identifier_str_mv |
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 107, n. 8, p. 969-977, 2012. 0074-0276 S0074-02762012000800002.pdf S0074-02762012000800002 10.1590/S0074-02762012000800002 WOS:000328105100002 |
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http://repositorio.unifesp.br/handle/11600/7448 http://dx.doi.org/10.1590/S0074-02762012000800002 |
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969-977 |
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Instituto Oswaldo Cruz, Ministério da Saúde |
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Instituto Oswaldo Cruz, Ministério da Saúde |
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