The blockade of cyclooxygenases-1 and-2 reduces the effects of hypoxia on endothelial cells

Detalhes bibliográficos
Autor(a) principal: Gloria, Maria Aparecida da [UNIFESP]
Data de Publicação: 2006
Outros Autores: Cenedeze, Marcos Antonio [UNIFESP], Pacheco-Silva, Alvaro [UNIFESP], Camara, Niels Olsen Saraiva [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0100-879X2006000900006
http://repositorio.unifesp.br/handle/11600/29119
Resumo: Hypoxia activates endothelial cells by the action of reactive oxygen species generated in part by cyclooxygenases (COX) production enhancing leukocyte transmigration. We investigated the effect of specific COX inhibition on the function of endothelial cells exposed to hypoxia. Mouse immortalized endothelial cells were subjected to 30 min of oxygen deprivation by gas exchange. Acridine orange/ ethidium bromide dyes and lactate dehydrogenase activity were used to monitor cell viability. the mRNA of COX-1 and -2 was amplified and semi-quantified before and after hypoxia in cells treated or not with indomethacin, a non-selective COX inhibitor. Expression of RANTES ( regulated upon activation, normal T cell expressed and secreted) protein and the protective role of heme oxygenase-1 (HO-1) were also investigated by PCR. Gas exchange decreased partial oxygen pressure (PaO2) by 45.12 +/- 5.85% (from 162 +/- 10 to 73 +/- 7.4 mmIIg). Thirty minutes of hypoxia decreased cell viability and enhanced lactate dehydrogenase levels compared to control (73.1 +/- 2.7 vs 91.2 +/- 0.9%, P < 0.02; 35.96 +/- 11.64 vs 22.19 +/- 9.65%, P = 0.002, respectively). COX-2 and HO-1 mRNA were up-regulated after hypoxia. Indomethacin (300 mu M) decreased COX-2, HO-1, hypoxiainducible factor-1 alpha and RANTES mRNA and increased cell viability after hypoxia. We conclude that blockade of COX up-regulation can ameliorate endothelial injury, resulting in reduced production of chemokines.
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spelling The blockade of cyclooxygenases-1 and-2 reduces the effects of hypoxia on endothelial cellsendothelial cellhypoxiaindomethacincyclooxygenaseheme oxygenase 1Hypoxia activates endothelial cells by the action of reactive oxygen species generated in part by cyclooxygenases (COX) production enhancing leukocyte transmigration. We investigated the effect of specific COX inhibition on the function of endothelial cells exposed to hypoxia. Mouse immortalized endothelial cells were subjected to 30 min of oxygen deprivation by gas exchange. Acridine orange/ ethidium bromide dyes and lactate dehydrogenase activity were used to monitor cell viability. the mRNA of COX-1 and -2 was amplified and semi-quantified before and after hypoxia in cells treated or not with indomethacin, a non-selective COX inhibitor. Expression of RANTES ( regulated upon activation, normal T cell expressed and secreted) protein and the protective role of heme oxygenase-1 (HO-1) were also investigated by PCR. Gas exchange decreased partial oxygen pressure (PaO2) by 45.12 +/- 5.85% (from 162 +/- 10 to 73 +/- 7.4 mmIIg). Thirty minutes of hypoxia decreased cell viability and enhanced lactate dehydrogenase levels compared to control (73.1 +/- 2.7 vs 91.2 +/- 0.9%, P < 0.02; 35.96 +/- 11.64 vs 22.19 +/- 9.65%, P = 0.002, respectively). COX-2 and HO-1 mRNA were up-regulated after hypoxia. Indomethacin (300 mu M) decreased COX-2, HO-1, hypoxiainducible factor-1 alpha and RANTES mRNA and increased cell viability after hypoxia. We conclude that blockade of COX up-regulation can ameliorate endothelial injury, resulting in reduced production of chemokines.UNIFESP, Div Nefrol, Lab Imunol Clin & Expt, Hosp Rim & Hipertensao,Fundacao Oswaldo Ramos, São Paulo, SP, BrazilUniv São Paulo, Dept Imunol, Inst Ciencias Biomed 4, Lab Imunobiol Transplantes, São Paulo, SP, BrazilUNIFESP, Div Nefrol, Lab Imunol Clin & Expt, Hosp Rim & Hipertensao,Fundacao Oswaldo Ramos, São Paulo, SP, BrazilWeb of ScienceAssoc Bras Divulg CientificaUniversidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Gloria, Maria Aparecida da [UNIFESP]Cenedeze, Marcos Antonio [UNIFESP]Pacheco-Silva, Alvaro [UNIFESP]Camara, Niels Olsen Saraiva [UNIFESP]2016-01-24T12:41:25Z2016-01-24T12:41:25Z2006-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1189-1196application/pdfhttp://dx.doi.org/10.1590/S0100-879X2006000900006Brazilian Journal of Medical and Biological Research. São Paulo: Assoc Bras Divulg Cientifica, v. 39, n. 9, p. 1189-1196, 2006.10.1590/S0100-879X20060009000060100-879XS0100-879X2006000900006http://repositorio.unifesp.br/handle/11600/29119WOS:000240545900006engBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-31T03:48:11Zoai:repositorio.unifesp.br/:11600/29119Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-31T03:48:11Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv The blockade of cyclooxygenases-1 and-2 reduces the effects of hypoxia on endothelial cells
title The blockade of cyclooxygenases-1 and-2 reduces the effects of hypoxia on endothelial cells
spellingShingle The blockade of cyclooxygenases-1 and-2 reduces the effects of hypoxia on endothelial cells
Gloria, Maria Aparecida da [UNIFESP]
endothelial cell
hypoxia
indomethacin
cyclooxygenase
heme oxygenase 1
title_short The blockade of cyclooxygenases-1 and-2 reduces the effects of hypoxia on endothelial cells
title_full The blockade of cyclooxygenases-1 and-2 reduces the effects of hypoxia on endothelial cells
title_fullStr The blockade of cyclooxygenases-1 and-2 reduces the effects of hypoxia on endothelial cells
title_full_unstemmed The blockade of cyclooxygenases-1 and-2 reduces the effects of hypoxia on endothelial cells
title_sort The blockade of cyclooxygenases-1 and-2 reduces the effects of hypoxia on endothelial cells
author Gloria, Maria Aparecida da [UNIFESP]
author_facet Gloria, Maria Aparecida da [UNIFESP]
Cenedeze, Marcos Antonio [UNIFESP]
Pacheco-Silva, Alvaro [UNIFESP]
Camara, Niels Olsen Saraiva [UNIFESP]
author_role author
author2 Cenedeze, Marcos Antonio [UNIFESP]
Pacheco-Silva, Alvaro [UNIFESP]
Camara, Niels Olsen Saraiva [UNIFESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Gloria, Maria Aparecida da [UNIFESP]
Cenedeze, Marcos Antonio [UNIFESP]
Pacheco-Silva, Alvaro [UNIFESP]
Camara, Niels Olsen Saraiva [UNIFESP]
dc.subject.por.fl_str_mv endothelial cell
hypoxia
indomethacin
cyclooxygenase
heme oxygenase 1
topic endothelial cell
hypoxia
indomethacin
cyclooxygenase
heme oxygenase 1
description Hypoxia activates endothelial cells by the action of reactive oxygen species generated in part by cyclooxygenases (COX) production enhancing leukocyte transmigration. We investigated the effect of specific COX inhibition on the function of endothelial cells exposed to hypoxia. Mouse immortalized endothelial cells were subjected to 30 min of oxygen deprivation by gas exchange. Acridine orange/ ethidium bromide dyes and lactate dehydrogenase activity were used to monitor cell viability. the mRNA of COX-1 and -2 was amplified and semi-quantified before and after hypoxia in cells treated or not with indomethacin, a non-selective COX inhibitor. Expression of RANTES ( regulated upon activation, normal T cell expressed and secreted) protein and the protective role of heme oxygenase-1 (HO-1) were also investigated by PCR. Gas exchange decreased partial oxygen pressure (PaO2) by 45.12 +/- 5.85% (from 162 +/- 10 to 73 +/- 7.4 mmIIg). Thirty minutes of hypoxia decreased cell viability and enhanced lactate dehydrogenase levels compared to control (73.1 +/- 2.7 vs 91.2 +/- 0.9%, P < 0.02; 35.96 +/- 11.64 vs 22.19 +/- 9.65%, P = 0.002, respectively). COX-2 and HO-1 mRNA were up-regulated after hypoxia. Indomethacin (300 mu M) decreased COX-2, HO-1, hypoxiainducible factor-1 alpha and RANTES mRNA and increased cell viability after hypoxia. We conclude that blockade of COX up-regulation can ameliorate endothelial injury, resulting in reduced production of chemokines.
publishDate 2006
dc.date.none.fl_str_mv 2006-09-01
2016-01-24T12:41:25Z
2016-01-24T12:41:25Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-879X2006000900006
Brazilian Journal of Medical and Biological Research. São Paulo: Assoc Bras Divulg Cientifica, v. 39, n. 9, p. 1189-1196, 2006.
10.1590/S0100-879X2006000900006
0100-879X
S0100-879X2006000900006
http://repositorio.unifesp.br/handle/11600/29119
WOS:000240545900006
url http://dx.doi.org/10.1590/S0100-879X2006000900006
http://repositorio.unifesp.br/handle/11600/29119
identifier_str_mv Brazilian Journal of Medical and Biological Research. São Paulo: Assoc Bras Divulg Cientifica, v. 39, n. 9, p. 1189-1196, 2006.
10.1590/S0100-879X2006000900006
0100-879X
S0100-879X2006000900006
WOS:000240545900006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Medical and Biological Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1189-1196
application/pdf
dc.publisher.none.fl_str_mv Assoc Bras Divulg Cientifica
publisher.none.fl_str_mv Assoc Bras Divulg Cientifica
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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