The blockade of cyclooxygenases-1 and-2 reduces the effects of hypoxia on endothelial cells
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1590/S0100-879X2006000900006 http://repositorio.unifesp.br/handle/11600/29119 |
Resumo: | Hypoxia activates endothelial cells by the action of reactive oxygen species generated in part by cyclooxygenases (COX) production enhancing leukocyte transmigration. We investigated the effect of specific COX inhibition on the function of endothelial cells exposed to hypoxia. Mouse immortalized endothelial cells were subjected to 30 min of oxygen deprivation by gas exchange. Acridine orange/ ethidium bromide dyes and lactate dehydrogenase activity were used to monitor cell viability. the mRNA of COX-1 and -2 was amplified and semi-quantified before and after hypoxia in cells treated or not with indomethacin, a non-selective COX inhibitor. Expression of RANTES ( regulated upon activation, normal T cell expressed and secreted) protein and the protective role of heme oxygenase-1 (HO-1) were also investigated by PCR. Gas exchange decreased partial oxygen pressure (PaO2) by 45.12 +/- 5.85% (from 162 +/- 10 to 73 +/- 7.4 mmIIg). Thirty minutes of hypoxia decreased cell viability and enhanced lactate dehydrogenase levels compared to control (73.1 +/- 2.7 vs 91.2 +/- 0.9%, P < 0.02; 35.96 +/- 11.64 vs 22.19 +/- 9.65%, P = 0.002, respectively). COX-2 and HO-1 mRNA were up-regulated after hypoxia. Indomethacin (300 mu M) decreased COX-2, HO-1, hypoxiainducible factor-1 alpha and RANTES mRNA and increased cell viability after hypoxia. We conclude that blockade of COX up-regulation can ameliorate endothelial injury, resulting in reduced production of chemokines. |
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The blockade of cyclooxygenases-1 and-2 reduces the effects of hypoxia on endothelial cellsendothelial cellhypoxiaindomethacincyclooxygenaseheme oxygenase 1Hypoxia activates endothelial cells by the action of reactive oxygen species generated in part by cyclooxygenases (COX) production enhancing leukocyte transmigration. We investigated the effect of specific COX inhibition on the function of endothelial cells exposed to hypoxia. Mouse immortalized endothelial cells were subjected to 30 min of oxygen deprivation by gas exchange. Acridine orange/ ethidium bromide dyes and lactate dehydrogenase activity were used to monitor cell viability. the mRNA of COX-1 and -2 was amplified and semi-quantified before and after hypoxia in cells treated or not with indomethacin, a non-selective COX inhibitor. Expression of RANTES ( regulated upon activation, normal T cell expressed and secreted) protein and the protective role of heme oxygenase-1 (HO-1) were also investigated by PCR. Gas exchange decreased partial oxygen pressure (PaO2) by 45.12 +/- 5.85% (from 162 +/- 10 to 73 +/- 7.4 mmIIg). Thirty minutes of hypoxia decreased cell viability and enhanced lactate dehydrogenase levels compared to control (73.1 +/- 2.7 vs 91.2 +/- 0.9%, P < 0.02; 35.96 +/- 11.64 vs 22.19 +/- 9.65%, P = 0.002, respectively). COX-2 and HO-1 mRNA were up-regulated after hypoxia. Indomethacin (300 mu M) decreased COX-2, HO-1, hypoxiainducible factor-1 alpha and RANTES mRNA and increased cell viability after hypoxia. We conclude that blockade of COX up-regulation can ameliorate endothelial injury, resulting in reduced production of chemokines.UNIFESP, Div Nefrol, Lab Imunol Clin & Expt, Hosp Rim & Hipertensao,Fundacao Oswaldo Ramos, São Paulo, SP, BrazilUniv São Paulo, Dept Imunol, Inst Ciencias Biomed 4, Lab Imunobiol Transplantes, São Paulo, SP, BrazilUNIFESP, Div Nefrol, Lab Imunol Clin & Expt, Hosp Rim & Hipertensao,Fundacao Oswaldo Ramos, São Paulo, SP, BrazilWeb of ScienceAssoc Bras Divulg CientificaUniversidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Gloria, Maria Aparecida da [UNIFESP]Cenedeze, Marcos Antonio [UNIFESP]Pacheco-Silva, Alvaro [UNIFESP]Camara, Niels Olsen Saraiva [UNIFESP]2016-01-24T12:41:25Z2016-01-24T12:41:25Z2006-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1189-1196application/pdfhttp://dx.doi.org/10.1590/S0100-879X2006000900006Brazilian Journal of Medical and Biological Research. São Paulo: Assoc Bras Divulg Cientifica, v. 39, n. 9, p. 1189-1196, 2006.10.1590/S0100-879X20060009000060100-879XS0100-879X2006000900006http://repositorio.unifesp.br/handle/11600/29119WOS:000240545900006engBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-31T03:48:11Zoai:repositorio.unifesp.br/:11600/29119Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-31T03:48:11Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
The blockade of cyclooxygenases-1 and-2 reduces the effects of hypoxia on endothelial cells |
title |
The blockade of cyclooxygenases-1 and-2 reduces the effects of hypoxia on endothelial cells |
spellingShingle |
The blockade of cyclooxygenases-1 and-2 reduces the effects of hypoxia on endothelial cells Gloria, Maria Aparecida da [UNIFESP] endothelial cell hypoxia indomethacin cyclooxygenase heme oxygenase 1 |
title_short |
The blockade of cyclooxygenases-1 and-2 reduces the effects of hypoxia on endothelial cells |
title_full |
The blockade of cyclooxygenases-1 and-2 reduces the effects of hypoxia on endothelial cells |
title_fullStr |
The blockade of cyclooxygenases-1 and-2 reduces the effects of hypoxia on endothelial cells |
title_full_unstemmed |
The blockade of cyclooxygenases-1 and-2 reduces the effects of hypoxia on endothelial cells |
title_sort |
The blockade of cyclooxygenases-1 and-2 reduces the effects of hypoxia on endothelial cells |
author |
Gloria, Maria Aparecida da [UNIFESP] |
author_facet |
Gloria, Maria Aparecida da [UNIFESP] Cenedeze, Marcos Antonio [UNIFESP] Pacheco-Silva, Alvaro [UNIFESP] Camara, Niels Olsen Saraiva [UNIFESP] |
author_role |
author |
author2 |
Cenedeze, Marcos Antonio [UNIFESP] Pacheco-Silva, Alvaro [UNIFESP] Camara, Niels Olsen Saraiva [UNIFESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Gloria, Maria Aparecida da [UNIFESP] Cenedeze, Marcos Antonio [UNIFESP] Pacheco-Silva, Alvaro [UNIFESP] Camara, Niels Olsen Saraiva [UNIFESP] |
dc.subject.por.fl_str_mv |
endothelial cell hypoxia indomethacin cyclooxygenase heme oxygenase 1 |
topic |
endothelial cell hypoxia indomethacin cyclooxygenase heme oxygenase 1 |
description |
Hypoxia activates endothelial cells by the action of reactive oxygen species generated in part by cyclooxygenases (COX) production enhancing leukocyte transmigration. We investigated the effect of specific COX inhibition on the function of endothelial cells exposed to hypoxia. Mouse immortalized endothelial cells were subjected to 30 min of oxygen deprivation by gas exchange. Acridine orange/ ethidium bromide dyes and lactate dehydrogenase activity were used to monitor cell viability. the mRNA of COX-1 and -2 was amplified and semi-quantified before and after hypoxia in cells treated or not with indomethacin, a non-selective COX inhibitor. Expression of RANTES ( regulated upon activation, normal T cell expressed and secreted) protein and the protective role of heme oxygenase-1 (HO-1) were also investigated by PCR. Gas exchange decreased partial oxygen pressure (PaO2) by 45.12 +/- 5.85% (from 162 +/- 10 to 73 +/- 7.4 mmIIg). Thirty minutes of hypoxia decreased cell viability and enhanced lactate dehydrogenase levels compared to control (73.1 +/- 2.7 vs 91.2 +/- 0.9%, P < 0.02; 35.96 +/- 11.64 vs 22.19 +/- 9.65%, P = 0.002, respectively). COX-2 and HO-1 mRNA were up-regulated after hypoxia. Indomethacin (300 mu M) decreased COX-2, HO-1, hypoxiainducible factor-1 alpha and RANTES mRNA and increased cell viability after hypoxia. We conclude that blockade of COX up-regulation can ameliorate endothelial injury, resulting in reduced production of chemokines. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-09-01 2016-01-24T12:41:25Z 2016-01-24T12:41:25Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-879X2006000900006 Brazilian Journal of Medical and Biological Research. São Paulo: Assoc Bras Divulg Cientifica, v. 39, n. 9, p. 1189-1196, 2006. 10.1590/S0100-879X2006000900006 0100-879X S0100-879X2006000900006 http://repositorio.unifesp.br/handle/11600/29119 WOS:000240545900006 |
url |
http://dx.doi.org/10.1590/S0100-879X2006000900006 http://repositorio.unifesp.br/handle/11600/29119 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research. São Paulo: Assoc Bras Divulg Cientifica, v. 39, n. 9, p. 1189-1196, 2006. 10.1590/S0100-879X2006000900006 0100-879X S0100-879X2006000900006 WOS:000240545900006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1189-1196 application/pdf |
dc.publisher.none.fl_str_mv |
Assoc Bras Divulg Cientifica |
publisher.none.fl_str_mv |
Assoc Bras Divulg Cientifica |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
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1814268360841494528 |