Blood flow measurements in rats using four color microspheres during blockade of different vasopressor systems
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/28067 http://dx.doi.org/10.1590/S0100-879X2005000100018 |
Resumo: | The use of colored microspheres to adequately evaluate blood flow chancres under different circumstances in the same rat has been validated with a maximum of three different colors due to methodological limitations. the aim of the present study was to validate the use of four different colors measuring four repeated blood flow. changes in the same rat to assess the role of vasopressor systems in. controlling arterial pressure (AP). Red (150,000), white (200,000)), yellow (150,000), and blue (200,000) colored microspheres were infused into the left ventricle of 6 male Wistar rats 1) at rest and 2) after vasopressin (aAVP, 10 mug/kg, iv), 3) renin-angiotensin (losartan, 10 ms/kg iv), and 4) sympathetic system blockade (hexamethonium., 20 mg/kg, iv) to determine blood flow changes. AP was recorded and processed with a data acquisition system (1-kHz sampling frequency). Blood flow changes were quantified by spectrophotometry absorption peaks for colored microsphere components in the tissues evaluated. Administration of aAVP and losartan slightly reduced the AP (-5.7 +/- 0.5 and -7.8 +/- 1.2 mmHg, respectively), while hexamethonium induced a 52 +/- 3 mmHg fall in AP. the aAVP injection increased blood flow in lungs (78%), liver (117%) and skeletal muscle (>150%), while losartan administration enhanced blood flow in heart (126%), lungs (100%), kidneys (80%), and gastrocnemius (75%) and soleus (94%) muscles. Hexamethonium administration reduced only kidney blood flow (50%). in conclusion, four types of colored microspheres can be used to perform four repeated blood flow measurements in the same rat detecting small alterations such as changes in tissues with low blood flow. |
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De Angelis, KatiaGama, V. M. [UNIFESP]Farah, VAMIrigoyen, Maria Claudia [UNIFESP]Universidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)Univ Santo AmaroUniv Sao Judas Tadeu2016-01-24T12:37:32Z2016-01-24T12:37:32Z2005-01-01Brazilian Journal of Medical and Biological Research. São Paulo: Assoc Bras Divulg Cientifica, v. 38, n. 1, p. 119-125, 2005.0100-879Xhttp://repositorio.unifesp.br/handle/11600/28067http://dx.doi.org/10.1590/S0100-879X2005000100018S0100-879X200500010001810.1590/S0100-879X2005000100018WOS:000226663600018The use of colored microspheres to adequately evaluate blood flow chancres under different circumstances in the same rat has been validated with a maximum of three different colors due to methodological limitations. the aim of the present study was to validate the use of four different colors measuring four repeated blood flow. changes in the same rat to assess the role of vasopressor systems in. controlling arterial pressure (AP). Red (150,000), white (200,000)), yellow (150,000), and blue (200,000) colored microspheres were infused into the left ventricle of 6 male Wistar rats 1) at rest and 2) after vasopressin (aAVP, 10 mug/kg, iv), 3) renin-angiotensin (losartan, 10 ms/kg iv), and 4) sympathetic system blockade (hexamethonium., 20 mg/kg, iv) to determine blood flow changes. AP was recorded and processed with a data acquisition system (1-kHz sampling frequency). Blood flow changes were quantified by spectrophotometry absorption peaks for colored microsphere components in the tissues evaluated. Administration of aAVP and losartan slightly reduced the AP (-5.7 +/- 0.5 and -7.8 +/- 1.2 mmHg, respectively), while hexamethonium induced a 52 +/- 3 mmHg fall in AP. the aAVP injection increased blood flow in lungs (78%), liver (117%) and skeletal muscle (>150%), while losartan administration enhanced blood flow in heart (126%), lungs (100%), kidneys (80%), and gastrocnemius (75%) and soleus (94%) muscles. Hexamethonium administration reduced only kidney blood flow (50%). in conclusion, four types of colored microspheres can be used to perform four repeated blood flow measurements in the same rat detecting small alterations such as changes in tissues with low blood flow.Univ São Paulo, Fac Med, Inst Coracao, Unidade Hipertensao, BR-05403000 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Nefrol, São Paulo, BrazilUniv Santo Amaro, UNIPESQ, Santo Amaro, SP, BrazilUniv Sao Judas Tadeu, Lab Movimiento Humano, São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Nefrol, São Paulo, BrazilWeb of Science119-125engAssoc Bras Divulg CientificaBrazilian Journal of Medical and Biological Researchcolored microspheresarterial pressuresympathetic systemrenin-angiotensinvasopressinlocal blood flow measurementBlood flow measurements in rats using four color microspheres during blockade of different vasopressor systemsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS0100-879X2005000100018.pdfS0100-879X2005000100018.pdfapplication/pdf516253${dspace.ui.url}/bitstream/11600/28067/1/S0100-879X2005000100018.pdf2a3cbf2e47f9cedc8f07f71aa51ee9bcMD51open access11600/280672022-11-03 15:20:02.217open accessoai:repositorio.unifesp.br:11600/28067Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-11-03T18:20:02Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Blood flow measurements in rats using four color microspheres during blockade of different vasopressor systems |
title |
Blood flow measurements in rats using four color microspheres during blockade of different vasopressor systems |
spellingShingle |
Blood flow measurements in rats using four color microspheres during blockade of different vasopressor systems De Angelis, Katia colored microspheres arterial pressure sympathetic system renin-angiotensin vasopressin local blood flow measurement |
title_short |
Blood flow measurements in rats using four color microspheres during blockade of different vasopressor systems |
title_full |
Blood flow measurements in rats using four color microspheres during blockade of different vasopressor systems |
title_fullStr |
Blood flow measurements in rats using four color microspheres during blockade of different vasopressor systems |
title_full_unstemmed |
Blood flow measurements in rats using four color microspheres during blockade of different vasopressor systems |
title_sort |
Blood flow measurements in rats using four color microspheres during blockade of different vasopressor systems |
author |
De Angelis, Katia |
author_facet |
De Angelis, Katia Gama, V. M. [UNIFESP] Farah, VAM Irigoyen, Maria Claudia [UNIFESP] |
author_role |
author |
author2 |
Gama, V. M. [UNIFESP] Farah, VAM Irigoyen, Maria Claudia [UNIFESP] |
author2_role |
author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Federal de São Paulo (UNIFESP) Univ Santo Amaro Univ Sao Judas Tadeu |
dc.contributor.author.fl_str_mv |
De Angelis, Katia Gama, V. M. [UNIFESP] Farah, VAM Irigoyen, Maria Claudia [UNIFESP] |
dc.subject.eng.fl_str_mv |
colored microspheres arterial pressure sympathetic system renin-angiotensin vasopressin local blood flow measurement |
topic |
colored microspheres arterial pressure sympathetic system renin-angiotensin vasopressin local blood flow measurement |
description |
The use of colored microspheres to adequately evaluate blood flow chancres under different circumstances in the same rat has been validated with a maximum of three different colors due to methodological limitations. the aim of the present study was to validate the use of four different colors measuring four repeated blood flow. changes in the same rat to assess the role of vasopressor systems in. controlling arterial pressure (AP). Red (150,000), white (200,000)), yellow (150,000), and blue (200,000) colored microspheres were infused into the left ventricle of 6 male Wistar rats 1) at rest and 2) after vasopressin (aAVP, 10 mug/kg, iv), 3) renin-angiotensin (losartan, 10 ms/kg iv), and 4) sympathetic system blockade (hexamethonium., 20 mg/kg, iv) to determine blood flow changes. AP was recorded and processed with a data acquisition system (1-kHz sampling frequency). Blood flow changes were quantified by spectrophotometry absorption peaks for colored microsphere components in the tissues evaluated. Administration of aAVP and losartan slightly reduced the AP (-5.7 +/- 0.5 and -7.8 +/- 1.2 mmHg, respectively), while hexamethonium induced a 52 +/- 3 mmHg fall in AP. the aAVP injection increased blood flow in lungs (78%), liver (117%) and skeletal muscle (>150%), while losartan administration enhanced blood flow in heart (126%), lungs (100%), kidneys (80%), and gastrocnemius (75%) and soleus (94%) muscles. Hexamethonium administration reduced only kidney blood flow (50%). in conclusion, four types of colored microspheres can be used to perform four repeated blood flow measurements in the same rat detecting small alterations such as changes in tissues with low blood flow. |
publishDate |
2005 |
dc.date.issued.fl_str_mv |
2005-01-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T12:37:32Z |
dc.date.available.fl_str_mv |
2016-01-24T12:37:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Brazilian Journal of Medical and Biological Research. São Paulo: Assoc Bras Divulg Cientifica, v. 38, n. 1, p. 119-125, 2005. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/28067 http://dx.doi.org/10.1590/S0100-879X2005000100018 |
dc.identifier.issn.none.fl_str_mv |
0100-879X |
dc.identifier.scielo.none.fl_str_mv |
S0100-879X2005000100018 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S0100-879X2005000100018 |
dc.identifier.wos.none.fl_str_mv |
WOS:000226663600018 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research. São Paulo: Assoc Bras Divulg Cientifica, v. 38, n. 1, p. 119-125, 2005. 0100-879X S0100-879X2005000100018 10.1590/S0100-879X2005000100018 WOS:000226663600018 |
url |
http://repositorio.unifesp.br/handle/11600/28067 http://dx.doi.org/10.1590/S0100-879X2005000100018 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
119-125 |
dc.publisher.none.fl_str_mv |
Assoc Bras Divulg Cientifica |
publisher.none.fl_str_mv |
Assoc Bras Divulg Cientifica |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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Universidade Federal de São Paulo (UNIFESP) |
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UNIFESP |
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UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP |
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