Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoprotein

Detalhes bibliográficos
Autor(a) principal: Passos, Eduardo Pandolfi
Data de Publicação: 1999
Outros Autores: Brugnara, L., Facin, A.C., Riffel, Alessandro, Lima, Geraldo Rodrigues de [UNIFESP], Freitas, Vilmon de [UNIFESP], Brandelli, Adriano
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: https://dx.doi.org/10.1590/S0100-879X1999000100009
https://repositorio.unifesp.br/handle/11600/725
Resumo: The effect of peritoneal fluid (PF) on the human sperm acrosome reaction (AR) was tested. Sperm was pre-incubated with PF and the AR was induced by calcium ionophore A23187 and a neoglycoprotein bearing N-acetylglycosamine residues (NGP). The AR induced by calcium ionophore was inhibited 40% by PF from controls (PFc) and 50% by PF from the endometriosis (PFe) group, but not by PF from infertile patients without endometriosis (PFi). No significant differences were found in the spontaneous AR. When the AR was induced by NGP, pre-incubation with PFc reduced (60%) the percentage of AR, while PFe and PFi caused no significant differences. The average rates of acrosome reactions obtained in control, NGP- and ionophore-treated sperm showed that NGP-induced exocytosis differed significantly between the PFc (11%) and PFe/PFi groups (17%), and the ionophore-induced AR was higher for PFi (33%) than PFc/PFe (25%). The incidence of the NGP-induced AR was reduced in the first hour of pre-incubation with PFc and remained nearly constant throughout 4 h of incubation. The present data indicate that PF possesses a protective factor which prevents premature AR.
id UFSP_bfe16c955be16b85e3a01ff08c74af51
oai_identifier_str oai:repositorio.unifesp.br/:11600/725
network_acronym_str UFSP
network_name_str Repositório Institucional da UNIFESP
repository_id_str 3465
spelling Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoproteinAcrosome reactionEndometriosisInfertilityPeritoneal fluidSpermThe effect of peritoneal fluid (PF) on the human sperm acrosome reaction (AR) was tested. Sperm was pre-incubated with PF and the AR was induced by calcium ionophore A23187 and a neoglycoprotein bearing N-acetylglycosamine residues (NGP). The AR induced by calcium ionophore was inhibited 40% by PF from controls (PFc) and 50% by PF from the endometriosis (PFe) group, but not by PF from infertile patients without endometriosis (PFi). No significant differences were found in the spontaneous AR. When the AR was induced by NGP, pre-incubation with PFc reduced (60%) the percentage of AR, while PFe and PFi caused no significant differences. The average rates of acrosome reactions obtained in control, NGP- and ionophore-treated sperm showed that NGP-induced exocytosis differed significantly between the PFc (11%) and PFe/PFi groups (17%), and the ionophore-induced AR was higher for PFi (33%) than PFc/PFe (25%). The incidence of the NGP-induced AR was reduced in the first hour of pre-incubation with PFc and remained nearly constant throughout 4 h of incubation. The present data indicate that PF possesses a protective factor which prevents premature AR.Hospital de Clínicas de Porto AlegreUniversidade Federal de São Paulo (UNIFESP)UNIFESP, EPM, São Paulo, BrazilSciELOAssociação Brasileira de Divulgação CientíficaHospital de Clínicas de Porto AlegreUniversidade Federal de São Paulo (UNIFESP)Passos, Eduardo PandolfiBrugnara, L.Facin, A.C.Riffel, AlessandroLima, Geraldo Rodrigues de [UNIFESP]Freitas, Vilmon de [UNIFESP]Brandelli, Adriano2015-06-14T13:24:49Z2015-06-14T13:24:49Z1999-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion59-65application/pdfhttps://dx.doi.org/10.1590/S0100-879X1999000100009Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 32, n. 1, p. 59-65, 1999.10.1590/S0100-879X1999000100009S0100-879X1999000100009.pdf0100-879XS0100-879X1999000100009https://repositorio.unifesp.br/handle/11600/725WOS:000078324100009engBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-05T05:23:52Zoai:repositorio.unifesp.br/:11600/725Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-05T05:23:52Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoprotein
title Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoprotein
spellingShingle Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoprotein
Passos, Eduardo Pandolfi
Acrosome reaction
Endometriosis
Infertility
Peritoneal fluid
Sperm
title_short Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoprotein
title_full Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoprotein
title_fullStr Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoprotein
title_full_unstemmed Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoprotein
title_sort Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoprotein
author Passos, Eduardo Pandolfi
author_facet Passos, Eduardo Pandolfi
Brugnara, L.
Facin, A.C.
Riffel, Alessandro
Lima, Geraldo Rodrigues de [UNIFESP]
Freitas, Vilmon de [UNIFESP]
Brandelli, Adriano
author_role author
author2 Brugnara, L.
Facin, A.C.
Riffel, Alessandro
Lima, Geraldo Rodrigues de [UNIFESP]
Freitas, Vilmon de [UNIFESP]
Brandelli, Adriano
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Hospital de Clínicas de Porto Alegre
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Passos, Eduardo Pandolfi
Brugnara, L.
Facin, A.C.
Riffel, Alessandro
Lima, Geraldo Rodrigues de [UNIFESP]
Freitas, Vilmon de [UNIFESP]
Brandelli, Adriano
dc.subject.por.fl_str_mv Acrosome reaction
Endometriosis
Infertility
Peritoneal fluid
Sperm
topic Acrosome reaction
Endometriosis
Infertility
Peritoneal fluid
Sperm
description The effect of peritoneal fluid (PF) on the human sperm acrosome reaction (AR) was tested. Sperm was pre-incubated with PF and the AR was induced by calcium ionophore A23187 and a neoglycoprotein bearing N-acetylglycosamine residues (NGP). The AR induced by calcium ionophore was inhibited 40% by PF from controls (PFc) and 50% by PF from the endometriosis (PFe) group, but not by PF from infertile patients without endometriosis (PFi). No significant differences were found in the spontaneous AR. When the AR was induced by NGP, pre-incubation with PFc reduced (60%) the percentage of AR, while PFe and PFi caused no significant differences. The average rates of acrosome reactions obtained in control, NGP- and ionophore-treated sperm showed that NGP-induced exocytosis differed significantly between the PFc (11%) and PFe/PFi groups (17%), and the ionophore-induced AR was higher for PFi (33%) than PFc/PFe (25%). The incidence of the NGP-induced AR was reduced in the first hour of pre-incubation with PFc and remained nearly constant throughout 4 h of incubation. The present data indicate that PF possesses a protective factor which prevents premature AR.
publishDate 1999
dc.date.none.fl_str_mv 1999-01-01
2015-06-14T13:24:49Z
2015-06-14T13:24:49Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://dx.doi.org/10.1590/S0100-879X1999000100009
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 32, n. 1, p. 59-65, 1999.
10.1590/S0100-879X1999000100009
S0100-879X1999000100009.pdf
0100-879X
S0100-879X1999000100009
https://repositorio.unifesp.br/handle/11600/725
WOS:000078324100009
url https://dx.doi.org/10.1590/S0100-879X1999000100009
https://repositorio.unifesp.br/handle/11600/725
identifier_str_mv Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 32, n. 1, p. 59-65, 1999.
10.1590/S0100-879X1999000100009
S0100-879X1999000100009.pdf
0100-879X
S0100-879X1999000100009
WOS:000078324100009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Medical and Biological Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 59-65
application/pdf
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268349654237184