Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoprotein
Autor(a) principal: | |
---|---|
Data de Publicação: | 1999 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://dx.doi.org/10.1590/S0100-879X1999000100009 https://repositorio.unifesp.br/handle/11600/725 |
Resumo: | The effect of peritoneal fluid (PF) on the human sperm acrosome reaction (AR) was tested. Sperm was pre-incubated with PF and the AR was induced by calcium ionophore A23187 and a neoglycoprotein bearing N-acetylglycosamine residues (NGP). The AR induced by calcium ionophore was inhibited 40% by PF from controls (PFc) and 50% by PF from the endometriosis (PFe) group, but not by PF from infertile patients without endometriosis (PFi). No significant differences were found in the spontaneous AR. When the AR was induced by NGP, pre-incubation with PFc reduced (60%) the percentage of AR, while PFe and PFi caused no significant differences. The average rates of acrosome reactions obtained in control, NGP- and ionophore-treated sperm showed that NGP-induced exocytosis differed significantly between the PFc (11%) and PFe/PFi groups (17%), and the ionophore-induced AR was higher for PFi (33%) than PFc/PFe (25%). The incidence of the NGP-induced AR was reduced in the first hour of pre-incubation with PFc and remained nearly constant throughout 4 h of incubation. The present data indicate that PF possesses a protective factor which prevents premature AR. |
id |
UFSP_bfe16c955be16b85e3a01ff08c74af51 |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br/:11600/725 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoproteinAcrosome reactionEndometriosisInfertilityPeritoneal fluidSpermThe effect of peritoneal fluid (PF) on the human sperm acrosome reaction (AR) was tested. Sperm was pre-incubated with PF and the AR was induced by calcium ionophore A23187 and a neoglycoprotein bearing N-acetylglycosamine residues (NGP). The AR induced by calcium ionophore was inhibited 40% by PF from controls (PFc) and 50% by PF from the endometriosis (PFe) group, but not by PF from infertile patients without endometriosis (PFi). No significant differences were found in the spontaneous AR. When the AR was induced by NGP, pre-incubation with PFc reduced (60%) the percentage of AR, while PFe and PFi caused no significant differences. The average rates of acrosome reactions obtained in control, NGP- and ionophore-treated sperm showed that NGP-induced exocytosis differed significantly between the PFc (11%) and PFe/PFi groups (17%), and the ionophore-induced AR was higher for PFi (33%) than PFc/PFe (25%). The incidence of the NGP-induced AR was reduced in the first hour of pre-incubation with PFc and remained nearly constant throughout 4 h of incubation. The present data indicate that PF possesses a protective factor which prevents premature AR.Hospital de Clínicas de Porto AlegreUniversidade Federal de São Paulo (UNIFESP)UNIFESP, EPM, São Paulo, BrazilSciELOAssociação Brasileira de Divulgação CientíficaHospital de Clínicas de Porto AlegreUniversidade Federal de São Paulo (UNIFESP)Passos, Eduardo PandolfiBrugnara, L.Facin, A.C.Riffel, AlessandroLima, Geraldo Rodrigues de [UNIFESP]Freitas, Vilmon de [UNIFESP]Brandelli, Adriano2015-06-14T13:24:49Z2015-06-14T13:24:49Z1999-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion59-65application/pdfhttps://dx.doi.org/10.1590/S0100-879X1999000100009Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 32, n. 1, p. 59-65, 1999.10.1590/S0100-879X1999000100009S0100-879X1999000100009.pdf0100-879XS0100-879X1999000100009https://repositorio.unifesp.br/handle/11600/725WOS:000078324100009engBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-05T05:23:52Zoai:repositorio.unifesp.br/:11600/725Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-05T05:23:52Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoprotein |
title |
Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoprotein |
spellingShingle |
Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoprotein Passos, Eduardo Pandolfi Acrosome reaction Endometriosis Infertility Peritoneal fluid Sperm |
title_short |
Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoprotein |
title_full |
Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoprotein |
title_fullStr |
Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoprotein |
title_full_unstemmed |
Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoprotein |
title_sort |
Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoprotein |
author |
Passos, Eduardo Pandolfi |
author_facet |
Passos, Eduardo Pandolfi Brugnara, L. Facin, A.C. Riffel, Alessandro Lima, Geraldo Rodrigues de [UNIFESP] Freitas, Vilmon de [UNIFESP] Brandelli, Adriano |
author_role |
author |
author2 |
Brugnara, L. Facin, A.C. Riffel, Alessandro Lima, Geraldo Rodrigues de [UNIFESP] Freitas, Vilmon de [UNIFESP] Brandelli, Adriano |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Hospital de Clínicas de Porto Alegre Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Passos, Eduardo Pandolfi Brugnara, L. Facin, A.C. Riffel, Alessandro Lima, Geraldo Rodrigues de [UNIFESP] Freitas, Vilmon de [UNIFESP] Brandelli, Adriano |
dc.subject.por.fl_str_mv |
Acrosome reaction Endometriosis Infertility Peritoneal fluid Sperm |
topic |
Acrosome reaction Endometriosis Infertility Peritoneal fluid Sperm |
description |
The effect of peritoneal fluid (PF) on the human sperm acrosome reaction (AR) was tested. Sperm was pre-incubated with PF and the AR was induced by calcium ionophore A23187 and a neoglycoprotein bearing N-acetylglycosamine residues (NGP). The AR induced by calcium ionophore was inhibited 40% by PF from controls (PFc) and 50% by PF from the endometriosis (PFe) group, but not by PF from infertile patients without endometriosis (PFi). No significant differences were found in the spontaneous AR. When the AR was induced by NGP, pre-incubation with PFc reduced (60%) the percentage of AR, while PFe and PFi caused no significant differences. The average rates of acrosome reactions obtained in control, NGP- and ionophore-treated sperm showed that NGP-induced exocytosis differed significantly between the PFc (11%) and PFe/PFi groups (17%), and the ionophore-induced AR was higher for PFi (33%) than PFc/PFe (25%). The incidence of the NGP-induced AR was reduced in the first hour of pre-incubation with PFc and remained nearly constant throughout 4 h of incubation. The present data indicate that PF possesses a protective factor which prevents premature AR. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999-01-01 2015-06-14T13:24:49Z 2015-06-14T13:24:49Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://dx.doi.org/10.1590/S0100-879X1999000100009 Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 32, n. 1, p. 59-65, 1999. 10.1590/S0100-879X1999000100009 S0100-879X1999000100009.pdf 0100-879X S0100-879X1999000100009 https://repositorio.unifesp.br/handle/11600/725 WOS:000078324100009 |
url |
https://dx.doi.org/10.1590/S0100-879X1999000100009 https://repositorio.unifesp.br/handle/11600/725 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 32, n. 1, p. 59-65, 1999. 10.1590/S0100-879X1999000100009 S0100-879X1999000100009.pdf 0100-879X S0100-879X1999000100009 WOS:000078324100009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
59-65 application/pdf |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268349654237184 |