Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells

Detalhes bibliográficos
Autor(a) principal: Merjan, A.j. [UNIFESP]
Data de Publicação: 2001
Outros Autores: Kanashiro, Celia Akemi [UNIFESP], Krieger, Jose Eduardo, Han, Sang Won [UNIFESP], Paiva, Antonio Cechelli de Mattos [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0100-879X2001000900011
http://repositorio.unifesp.br/handle/11600/1236
Resumo: A construct (AT1R-NF) containing a Flag sequence added to the N-terminus of the rat AT1 receptor was stably expressed in Chinese hamster ovary cells and quantified in the cell membrane by confocal microscopy after reaction with a fluorescein-labeled anti-Flag monoclonal antibody. Angiotensin II bound to AT1R-NF and induced endocytosis with a half-time of 2 min. After 60-90 min, fluorescence accumulated around the cell nucleus, suggesting migration of the ligand-receptor complex to the nuclear membrane. Angiotensin antagonists also induced endocytosis, suggesting that a common step in the transduction signal mechanism occurring after ligand binding may be responsible for the ligand-receptor complex internalization.
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spelling Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cellsangiotensin IIagonist-induced receptor internalizationendocytosisfluorescence-tagged receptornuclear angiotensin receptorsrenin-angiotensin systemA construct (AT1R-NF) containing a Flag sequence added to the N-terminus of the rat AT1 receptor was stably expressed in Chinese hamster ovary cells and quantified in the cell membrane by confocal microscopy after reaction with a fluorescein-labeled anti-Flag monoclonal antibody. Angiotensin II bound to AT1R-NF and induced endocytosis with a half-time of 2 min. After 60-90 min, fluorescence accumulated around the cell nucleus, suggesting migration of the ligand-receptor complex to the nuclear membrane. Angiotensin antagonists also induced endocytosis, suggesting that a common step in the transduction signal mechanism occurring after ligand binding may be responsible for the ligand-receptor complex internalization.Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de BiofísicaUniversidade de São Paulo Faculdade de Medicina Instituto do CoraçãoUNIFESP, EPM, Depto. de BiofísicaSciELOAssociação Brasileira de Divulgação CientíficaUniversidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Merjan, A.j. [UNIFESP]Kanashiro, Celia Akemi [UNIFESP]Krieger, Jose EduardoHan, Sang Won [UNIFESP]Paiva, Antonio Cechelli de Mattos [UNIFESP]2015-06-14T13:29:29Z2015-06-14T13:29:29Z2001-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1175-1183application/pdfhttp://dx.doi.org/10.1590/S0100-879X2001000900011Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 9, p. 1175-1183, 2001.10.1590/S0100-879X2001000900011S0100-879X2001000900011.pdf0100-879XS0100-879X2001000900011http://repositorio.unifesp.br/handle/11600/1236WOS:000171117600011engBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-29T17:25:43Zoai:repositorio.unifesp.br/:11600/1236Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-29T17:25:43Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells
title Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells
spellingShingle Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells
Merjan, A.j. [UNIFESP]
angiotensin II
agonist-induced receptor internalization
endocytosis
fluorescence-tagged receptor
nuclear angiotensin receptors
renin-angiotensin system
title_short Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells
title_full Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells
title_fullStr Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells
title_full_unstemmed Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells
title_sort Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells
author Merjan, A.j. [UNIFESP]
author_facet Merjan, A.j. [UNIFESP]
Kanashiro, Celia Akemi [UNIFESP]
Krieger, Jose Eduardo
Han, Sang Won [UNIFESP]
Paiva, Antonio Cechelli de Mattos [UNIFESP]
author_role author
author2 Kanashiro, Celia Akemi [UNIFESP]
Krieger, Jose Eduardo
Han, Sang Won [UNIFESP]
Paiva, Antonio Cechelli de Mattos [UNIFESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Merjan, A.j. [UNIFESP]
Kanashiro, Celia Akemi [UNIFESP]
Krieger, Jose Eduardo
Han, Sang Won [UNIFESP]
Paiva, Antonio Cechelli de Mattos [UNIFESP]
dc.subject.por.fl_str_mv angiotensin II
agonist-induced receptor internalization
endocytosis
fluorescence-tagged receptor
nuclear angiotensin receptors
renin-angiotensin system
topic angiotensin II
agonist-induced receptor internalization
endocytosis
fluorescence-tagged receptor
nuclear angiotensin receptors
renin-angiotensin system
description A construct (AT1R-NF) containing a Flag sequence added to the N-terminus of the rat AT1 receptor was stably expressed in Chinese hamster ovary cells and quantified in the cell membrane by confocal microscopy after reaction with a fluorescein-labeled anti-Flag monoclonal antibody. Angiotensin II bound to AT1R-NF and induced endocytosis with a half-time of 2 min. After 60-90 min, fluorescence accumulated around the cell nucleus, suggesting migration of the ligand-receptor complex to the nuclear membrane. Angiotensin antagonists also induced endocytosis, suggesting that a common step in the transduction signal mechanism occurring after ligand binding may be responsible for the ligand-receptor complex internalization.
publishDate 2001
dc.date.none.fl_str_mv 2001-09-01
2015-06-14T13:29:29Z
2015-06-14T13:29:29Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-879X2001000900011
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 9, p. 1175-1183, 2001.
10.1590/S0100-879X2001000900011
S0100-879X2001000900011.pdf
0100-879X
S0100-879X2001000900011
http://repositorio.unifesp.br/handle/11600/1236
WOS:000171117600011
url http://dx.doi.org/10.1590/S0100-879X2001000900011
http://repositorio.unifesp.br/handle/11600/1236
identifier_str_mv Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 9, p. 1175-1183, 2001.
10.1590/S0100-879X2001000900011
S0100-879X2001000900011.pdf
0100-879X
S0100-879X2001000900011
WOS:000171117600011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Medical and Biological Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1175-1183
application/pdf
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268355018752000

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