Search for LT-II and STb DNA Sequences Among Escherichia coli Isolated from Bovine Meat Products by Colony Hybridization

Detalhes bibliográficos
Autor(a) principal: Cerqueira, Aloysio de Mello Figueiredo
Data de Publicação: 1994
Outros Autores: Tibana, Anita, Gomes, Tania Aparecida Tardelli [UNIFESP], Guth, Beatriz Ernestina Cabilio [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: https://dx.doi.org/10.4315/0362-028X-57.8.734
https://repositorio.unifesp.br/handle/11600/44364
Resumo: A total of 1,066 Escherichia call colonies isolated from 105 raw bovine meat samples purchased at supermarkets in Rio de Janeiro were submitted to hybridization assays with gene probes for LT-II and STb enterotoxins. Five colonies comprising four different E. call strains isolated from four pieces of beef, two samples of ground beef (5.7%) and two hamburger patties (5.7%) hybridized with the LT-II probe, while no hybridization occurred with the STb probe. Expression of LT-II enterotoxin using the Y1 adrenal cell assay was verified in two of four E. coli strains. A serotype diversity existed among LT-II E. call strains.
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spelling Search for LT-II and STb DNA Sequences Among Escherichia coli Isolated from Bovine Meat Products by Colony HybridizationLT-IISTBESCHERICHIA COLIBOVINE MEATA total of 1,066 Escherichia call colonies isolated from 105 raw bovine meat samples purchased at supermarkets in Rio de Janeiro were submitted to hybridization assays with gene probes for LT-II and STb enterotoxins. Five colonies comprising four different E. call strains isolated from four pieces of beef, two samples of ground beef (5.7%) and two hamburger patties (5.7%) hybridized with the LT-II probe, while no hybridization occurred with the STb probe. Expression of LT-II enterotoxin using the Y1 adrenal cell assay was verified in two of four E. coli strains. A serotype diversity existed among LT-II E. call strains.ESCOLA PAULISTA MED, DEPT MICROBIOL IMMUNOL & PARASITOL, BR-04023 SAO PAULO, BRAZILUNIV FED RIO DE JANEIRO, INST MICROBIOL, DEPT MED MICROBIOL, BR-21941 RIO DE JANEIRO, BRAZILESCOLA PAULISTA MED, DEPT MICROBIOL IMMUNOL & PARASITOL, BR-04023 SAO PAULO, BRAZILWeb of ScienceInt Assoc Food ProtectionUniversidade Federal de São Paulo (UNIFESP)Universidade Federal do Rio de Janeiro (UFRJ)Cerqueira, Aloysio de Mello FigueiredoTibana, AnitaGomes, Tania Aparecida Tardelli [UNIFESP]Guth, Beatriz Ernestina Cabilio [UNIFESP]2018-06-15T17:58:46Z2018-06-15T17:58:46Z1994-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion734-736https://dx.doi.org/10.4315/0362-028X-57.8.734Journal Of Food Protection. Des Moines: Int Assoc Food Protection, v. 57, n. 8, p. 734-736, 1994.10.4315/0362-028X-57.8.7340362-028Xhttps://repositorio.unifesp.br/handle/11600/44364WOS:A1994PF22300015engJournal Of Food Protectioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-05-09T09:19:43Zoai:repositorio.unifesp.br/:11600/44364Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-05-09T09:19:43Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Search for LT-II and STb DNA Sequences Among Escherichia coli Isolated from Bovine Meat Products by Colony Hybridization
title Search for LT-II and STb DNA Sequences Among Escherichia coli Isolated from Bovine Meat Products by Colony Hybridization
spellingShingle Search for LT-II and STb DNA Sequences Among Escherichia coli Isolated from Bovine Meat Products by Colony Hybridization
Cerqueira, Aloysio de Mello Figueiredo
LT-II
STB
ESCHERICHIA COLI
BOVINE MEAT
title_short Search for LT-II and STb DNA Sequences Among Escherichia coli Isolated from Bovine Meat Products by Colony Hybridization
title_full Search for LT-II and STb DNA Sequences Among Escherichia coli Isolated from Bovine Meat Products by Colony Hybridization
title_fullStr Search for LT-II and STb DNA Sequences Among Escherichia coli Isolated from Bovine Meat Products by Colony Hybridization
title_full_unstemmed Search for LT-II and STb DNA Sequences Among Escherichia coli Isolated from Bovine Meat Products by Colony Hybridization
title_sort Search for LT-II and STb DNA Sequences Among Escherichia coli Isolated from Bovine Meat Products by Colony Hybridization
author Cerqueira, Aloysio de Mello Figueiredo
author_facet Cerqueira, Aloysio de Mello Figueiredo
Tibana, Anita
Gomes, Tania Aparecida Tardelli [UNIFESP]
Guth, Beatriz Ernestina Cabilio [UNIFESP]
author_role author
author2 Tibana, Anita
Gomes, Tania Aparecida Tardelli [UNIFESP]
Guth, Beatriz Ernestina Cabilio [UNIFESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.author.fl_str_mv Cerqueira, Aloysio de Mello Figueiredo
Tibana, Anita
Gomes, Tania Aparecida Tardelli [UNIFESP]
Guth, Beatriz Ernestina Cabilio [UNIFESP]
dc.subject.por.fl_str_mv LT-II
STB
ESCHERICHIA COLI
BOVINE MEAT
topic LT-II
STB
ESCHERICHIA COLI
BOVINE MEAT
description A total of 1,066 Escherichia call colonies isolated from 105 raw bovine meat samples purchased at supermarkets in Rio de Janeiro were submitted to hybridization assays with gene probes for LT-II and STb enterotoxins. Five colonies comprising four different E. call strains isolated from four pieces of beef, two samples of ground beef (5.7%) and two hamburger patties (5.7%) hybridized with the LT-II probe, while no hybridization occurred with the STb probe. Expression of LT-II enterotoxin using the Y1 adrenal cell assay was verified in two of four E. coli strains. A serotype diversity existed among LT-II E. call strains.
publishDate 1994
dc.date.none.fl_str_mv 1994-08-01
2018-06-15T17:58:46Z
2018-06-15T17:58:46Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://dx.doi.org/10.4315/0362-028X-57.8.734
Journal Of Food Protection. Des Moines: Int Assoc Food Protection, v. 57, n. 8, p. 734-736, 1994.
10.4315/0362-028X-57.8.734
0362-028X
https://repositorio.unifesp.br/handle/11600/44364
WOS:A1994PF22300015
url https://dx.doi.org/10.4315/0362-028X-57.8.734
https://repositorio.unifesp.br/handle/11600/44364
identifier_str_mv Journal Of Food Protection. Des Moines: Int Assoc Food Protection, v. 57, n. 8, p. 734-736, 1994.
10.4315/0362-028X-57.8.734
0362-028X
WOS:A1994PF22300015
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal Of Food Protection
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 734-736
dc.publisher.none.fl_str_mv Int Assoc Food Protection
publisher.none.fl_str_mv Int Assoc Food Protection
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1826890222567161856

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