Comparative Study of the Standard Fluorescent Antibody to Membrane Antigen (FAMA) Assay and a Flow Cytometry-Adapted FAMA Assay To Assess Immunity to Varicella-Zoster Virus

Detalhes bibliográficos
Autor(a) principal: Lafer, Manuel Mindlin [UNIFESP]
Data de Publicação: 2011
Outros Autores: Weckx, L. Y. [UNIFESP], Moraes-Pinto, M. I. de [UNIFESP], Garretson, A., Steinberg, S. P., Gershon, A. A., LaRussa, P. S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://repositorio.unifesp.br/handle/11600/33828
http://dx.doi.org/10.1128/CVI.05130-11
Resumo: A flow cytometry-adapted fluorescent antibody to membrane antigen (FAMA) assay to detect IgG antibodies against varicella-zoster virus (VZV) was developed and tested in 62 serum samples, showing 90.32% accuracy obtained from a receiver operating characteristic (ROC) curve with a 0.9125 (95% confidence interval [CI], 0.829 to 1.00) area below the curve compared to the result with standard FAMA.
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spelling Lafer, Manuel Mindlin [UNIFESP]Weckx, L. Y. [UNIFESP]Moraes-Pinto, M. I. de [UNIFESP]Garretson, A.Steinberg, S. P.Gershon, A. A.LaRussa, P. S.Universidade Federal de São Paulo (UNIFESP)Columbia Univ2016-01-24T14:16:55Z2016-01-24T14:16:55Z2011-07-01Clinical and Vaccine Immunology. Washington: Amer Soc Microbiology, v. 18, n. 7, p. 1194-1197, 2011.1556-6811http://repositorio.unifesp.br/handle/11600/33828http://dx.doi.org/10.1128/CVI.05130-11WOS000292378200023.pdf10.1128/CVI.05130-11WOS:000292378200023A flow cytometry-adapted fluorescent antibody to membrane antigen (FAMA) assay to detect IgG antibodies against varicella-zoster virus (VZV) was developed and tested in 62 serum samples, showing 90.32% accuracy obtained from a receiver operating characteristic (ROC) curve with a 0.9125 (95% confidence interval [CI], 0.829 to 1.00) area below the curve compared to the result with standard FAMA.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fed Univ São Paulo UNIFESP EPM, Div Pediat Infect Dis, BR-04040000 São Paulo, BrazilColumbia Univ, Dept Pediat, Coll Phys & Surg, New York, NY 10027 USAFed Univ São Paulo UNIFESP EPM, Div Pediat Infect Dis, BR-04040000 São Paulo, BrazilCAPES: 0108-08-1Web of Science1194-1197engAmer Soc MicrobiologyClinical and Vaccine ImmunologyComparative Study of the Standard Fluorescent Antibody to Membrane Antigen (FAMA) Assay and a Flow Cytometry-Adapted FAMA Assay To Assess Immunity to Varicella-Zoster Virusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000292378200023.pdfapplication/pdf458288${dspace.ui.url}/bitstream/11600/33828/1/WOS000292378200023.pdfaedb5539b1dda960c301df6c1e831d7aMD51open accessTEXTWOS000292378200023.pdf.txtWOS000292378200023.pdf.txtExtracted texttext/plain15254${dspace.ui.url}/bitstream/11600/33828/21/WOS000292378200023.pdf.txteecb4f4f83ad2d61ad978dda3c4a6a9aMD521open accessTHUMBNAILWOS000292378200023.pdf.jpgWOS000292378200023.pdf.jpgIM Thumbnailimage/jpeg6946${dspace.ui.url}/bitstream/11600/33828/23/WOS000292378200023.pdf.jpg004ed6a80283c64d8b185d419e5e0239MD523open access11600/338282023-06-05 19:09:52.29open accessoai:repositorio.unifesp.br:11600/33828Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:09:52Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.en.fl_str_mv Comparative Study of the Standard Fluorescent Antibody to Membrane Antigen (FAMA) Assay and a Flow Cytometry-Adapted FAMA Assay To Assess Immunity to Varicella-Zoster Virus
title Comparative Study of the Standard Fluorescent Antibody to Membrane Antigen (FAMA) Assay and a Flow Cytometry-Adapted FAMA Assay To Assess Immunity to Varicella-Zoster Virus
spellingShingle Comparative Study of the Standard Fluorescent Antibody to Membrane Antigen (FAMA) Assay and a Flow Cytometry-Adapted FAMA Assay To Assess Immunity to Varicella-Zoster Virus
Lafer, Manuel Mindlin [UNIFESP]
title_short Comparative Study of the Standard Fluorescent Antibody to Membrane Antigen (FAMA) Assay and a Flow Cytometry-Adapted FAMA Assay To Assess Immunity to Varicella-Zoster Virus
title_full Comparative Study of the Standard Fluorescent Antibody to Membrane Antigen (FAMA) Assay and a Flow Cytometry-Adapted FAMA Assay To Assess Immunity to Varicella-Zoster Virus
title_fullStr Comparative Study of the Standard Fluorescent Antibody to Membrane Antigen (FAMA) Assay and a Flow Cytometry-Adapted FAMA Assay To Assess Immunity to Varicella-Zoster Virus
title_full_unstemmed Comparative Study of the Standard Fluorescent Antibody to Membrane Antigen (FAMA) Assay and a Flow Cytometry-Adapted FAMA Assay To Assess Immunity to Varicella-Zoster Virus
title_sort Comparative Study of the Standard Fluorescent Antibody to Membrane Antigen (FAMA) Assay and a Flow Cytometry-Adapted FAMA Assay To Assess Immunity to Varicella-Zoster Virus
author Lafer, Manuel Mindlin [UNIFESP]
author_facet Lafer, Manuel Mindlin [UNIFESP]
Weckx, L. Y. [UNIFESP]
Moraes-Pinto, M. I. de [UNIFESP]
Garretson, A.
Steinberg, S. P.
Gershon, A. A.
LaRussa, P. S.
author_role author
author2 Weckx, L. Y. [UNIFESP]
Moraes-Pinto, M. I. de [UNIFESP]
Garretson, A.
Steinberg, S. P.
Gershon, A. A.
LaRussa, P. S.
author2_role author
author
author
author
author
author
dc.contributor.institution.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Columbia Univ
dc.contributor.author.fl_str_mv Lafer, Manuel Mindlin [UNIFESP]
Weckx, L. Y. [UNIFESP]
Moraes-Pinto, M. I. de [UNIFESP]
Garretson, A.
Steinberg, S. P.
Gershon, A. A.
LaRussa, P. S.
description A flow cytometry-adapted fluorescent antibody to membrane antigen (FAMA) assay to detect IgG antibodies against varicella-zoster virus (VZV) was developed and tested in 62 serum samples, showing 90.32% accuracy obtained from a receiver operating characteristic (ROC) curve with a 0.9125 (95% confidence interval [CI], 0.829 to 1.00) area below the curve compared to the result with standard FAMA.
publishDate 2011
dc.date.issued.fl_str_mv 2011-07-01
dc.date.accessioned.fl_str_mv 2016-01-24T14:16:55Z
dc.date.available.fl_str_mv 2016-01-24T14:16:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv Clinical and Vaccine Immunology. Washington: Amer Soc Microbiology, v. 18, n. 7, p. 1194-1197, 2011.
dc.identifier.uri.fl_str_mv http://repositorio.unifesp.br/handle/11600/33828
http://dx.doi.org/10.1128/CVI.05130-11
dc.identifier.issn.none.fl_str_mv 1556-6811
dc.identifier.file.none.fl_str_mv WOS000292378200023.pdf
dc.identifier.doi.none.fl_str_mv 10.1128/CVI.05130-11
dc.identifier.wos.none.fl_str_mv WOS:000292378200023
identifier_str_mv Clinical and Vaccine Immunology. Washington: Amer Soc Microbiology, v. 18, n. 7, p. 1194-1197, 2011.
1556-6811
WOS000292378200023.pdf
10.1128/CVI.05130-11
WOS:000292378200023
url http://repositorio.unifesp.br/handle/11600/33828
http://dx.doi.org/10.1128/CVI.05130-11
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dc.format.none.fl_str_mv 1194-1197
dc.publisher.none.fl_str_mv Amer Soc Microbiology
publisher.none.fl_str_mv Amer Soc Microbiology
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instname:Universidade Federal de São Paulo (UNIFESP)
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