Comparative Study of the Standard Fluorescent Antibody to Membrane Antigen (FAMA) Assay and a Flow Cytometry-Adapted FAMA Assay To Assess Immunity to Varicella-Zoster Virus
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/33828 http://dx.doi.org/10.1128/CVI.05130-11 |
Resumo: | A flow cytometry-adapted fluorescent antibody to membrane antigen (FAMA) assay to detect IgG antibodies against varicella-zoster virus (VZV) was developed and tested in 62 serum samples, showing 90.32% accuracy obtained from a receiver operating characteristic (ROC) curve with a 0.9125 (95% confidence interval [CI], 0.829 to 1.00) area below the curve compared to the result with standard FAMA. |
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Lafer, Manuel Mindlin [UNIFESP]Weckx, L. Y. [UNIFESP]Moraes-Pinto, M. I. de [UNIFESP]Garretson, A.Steinberg, S. P.Gershon, A. A.LaRussa, P. S.Universidade Federal de São Paulo (UNIFESP)Columbia Univ2016-01-24T14:16:55Z2016-01-24T14:16:55Z2011-07-01Clinical and Vaccine Immunology. Washington: Amer Soc Microbiology, v. 18, n. 7, p. 1194-1197, 2011.1556-6811http://repositorio.unifesp.br/handle/11600/33828http://dx.doi.org/10.1128/CVI.05130-11WOS000292378200023.pdf10.1128/CVI.05130-11WOS:000292378200023A flow cytometry-adapted fluorescent antibody to membrane antigen (FAMA) assay to detect IgG antibodies against varicella-zoster virus (VZV) was developed and tested in 62 serum samples, showing 90.32% accuracy obtained from a receiver operating characteristic (ROC) curve with a 0.9125 (95% confidence interval [CI], 0.829 to 1.00) area below the curve compared to the result with standard FAMA.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fed Univ São Paulo UNIFESP EPM, Div Pediat Infect Dis, BR-04040000 São Paulo, BrazilColumbia Univ, Dept Pediat, Coll Phys & Surg, New York, NY 10027 USAFed Univ São Paulo UNIFESP EPM, Div Pediat Infect Dis, BR-04040000 São Paulo, BrazilCAPES: 0108-08-1Web of Science1194-1197engAmer Soc MicrobiologyClinical and Vaccine ImmunologyComparative Study of the Standard Fluorescent Antibody to Membrane Antigen (FAMA) Assay and a Flow Cytometry-Adapted FAMA Assay To Assess Immunity to Varicella-Zoster Virusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000292378200023.pdfapplication/pdf458288${dspace.ui.url}/bitstream/11600/33828/1/WOS000292378200023.pdfaedb5539b1dda960c301df6c1e831d7aMD51open accessTEXTWOS000292378200023.pdf.txtWOS000292378200023.pdf.txtExtracted texttext/plain15254${dspace.ui.url}/bitstream/11600/33828/21/WOS000292378200023.pdf.txteecb4f4f83ad2d61ad978dda3c4a6a9aMD521open accessTHUMBNAILWOS000292378200023.pdf.jpgWOS000292378200023.pdf.jpgIM Thumbnailimage/jpeg6946${dspace.ui.url}/bitstream/11600/33828/23/WOS000292378200023.pdf.jpg004ed6a80283c64d8b185d419e5e0239MD523open access11600/338282023-06-05 19:09:52.29open accessoai:repositorio.unifesp.br:11600/33828Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:09:52Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Comparative Study of the Standard Fluorescent Antibody to Membrane Antigen (FAMA) Assay and a Flow Cytometry-Adapted FAMA Assay To Assess Immunity to Varicella-Zoster Virus |
title |
Comparative Study of the Standard Fluorescent Antibody to Membrane Antigen (FAMA) Assay and a Flow Cytometry-Adapted FAMA Assay To Assess Immunity to Varicella-Zoster Virus |
spellingShingle |
Comparative Study of the Standard Fluorescent Antibody to Membrane Antigen (FAMA) Assay and a Flow Cytometry-Adapted FAMA Assay To Assess Immunity to Varicella-Zoster Virus Lafer, Manuel Mindlin [UNIFESP] |
title_short |
Comparative Study of the Standard Fluorescent Antibody to Membrane Antigen (FAMA) Assay and a Flow Cytometry-Adapted FAMA Assay To Assess Immunity to Varicella-Zoster Virus |
title_full |
Comparative Study of the Standard Fluorescent Antibody to Membrane Antigen (FAMA) Assay and a Flow Cytometry-Adapted FAMA Assay To Assess Immunity to Varicella-Zoster Virus |
title_fullStr |
Comparative Study of the Standard Fluorescent Antibody to Membrane Antigen (FAMA) Assay and a Flow Cytometry-Adapted FAMA Assay To Assess Immunity to Varicella-Zoster Virus |
title_full_unstemmed |
Comparative Study of the Standard Fluorescent Antibody to Membrane Antigen (FAMA) Assay and a Flow Cytometry-Adapted FAMA Assay To Assess Immunity to Varicella-Zoster Virus |
title_sort |
Comparative Study of the Standard Fluorescent Antibody to Membrane Antigen (FAMA) Assay and a Flow Cytometry-Adapted FAMA Assay To Assess Immunity to Varicella-Zoster Virus |
author |
Lafer, Manuel Mindlin [UNIFESP] |
author_facet |
Lafer, Manuel Mindlin [UNIFESP] Weckx, L. Y. [UNIFESP] Moraes-Pinto, M. I. de [UNIFESP] Garretson, A. Steinberg, S. P. Gershon, A. A. LaRussa, P. S. |
author_role |
author |
author2 |
Weckx, L. Y. [UNIFESP] Moraes-Pinto, M. I. de [UNIFESP] Garretson, A. Steinberg, S. P. Gershon, A. A. LaRussa, P. S. |
author2_role |
author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Columbia Univ |
dc.contributor.author.fl_str_mv |
Lafer, Manuel Mindlin [UNIFESP] Weckx, L. Y. [UNIFESP] Moraes-Pinto, M. I. de [UNIFESP] Garretson, A. Steinberg, S. P. Gershon, A. A. LaRussa, P. S. |
description |
A flow cytometry-adapted fluorescent antibody to membrane antigen (FAMA) assay to detect IgG antibodies against varicella-zoster virus (VZV) was developed and tested in 62 serum samples, showing 90.32% accuracy obtained from a receiver operating characteristic (ROC) curve with a 0.9125 (95% confidence interval [CI], 0.829 to 1.00) area below the curve compared to the result with standard FAMA. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-07-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:16:55Z |
dc.date.available.fl_str_mv |
2016-01-24T14:16:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Clinical and Vaccine Immunology. Washington: Amer Soc Microbiology, v. 18, n. 7, p. 1194-1197, 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/33828 http://dx.doi.org/10.1128/CVI.05130-11 |
dc.identifier.issn.none.fl_str_mv |
1556-6811 |
dc.identifier.file.none.fl_str_mv |
WOS000292378200023.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1128/CVI.05130-11 |
dc.identifier.wos.none.fl_str_mv |
WOS:000292378200023 |
identifier_str_mv |
Clinical and Vaccine Immunology. Washington: Amer Soc Microbiology, v. 18, n. 7, p. 1194-1197, 2011. 1556-6811 WOS000292378200023.pdf 10.1128/CVI.05130-11 WOS:000292378200023 |
url |
http://repositorio.unifesp.br/handle/11600/33828 http://dx.doi.org/10.1128/CVI.05130-11 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Clinical and Vaccine Immunology |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
1194-1197 |
dc.publisher.none.fl_str_mv |
Amer Soc Microbiology |
publisher.none.fl_str_mv |
Amer Soc Microbiology |
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reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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Repositório Institucional da UNIFESP |
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