Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancer
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://dx.doi.org/10.1186/s12944-017-0547-x https://repositorio.unifesp.br/handle/11600/51379 |
Resumo: | Background: Cancer cachexia is a multifactorial metabolic syndrome characterized by marked loss of adipose tissue and skeletal muscle. Fat loss from adipose tissue in cancer cachexia is partly the result of increased lipolysis. Despite the growing amount of studies focused on elucidating the mechanisms through which lipolysis-related proteins regulate the lipolytic process, there are scarce data concerning that profile in the adipose tissue of cancer cachectic patients. Considering its fundamental importance, it was our main purpose to characterize the expression of the lipolysis-related proteins in the white adipose tissue of cachectic cancer patients. Methods: Patients from the University Hospital were divided into three groups: control, cancer cachexia (CC), and weight-stable cancer patients (WSC). To gain greater insight into adipose tissue wasting during cancer cachexia progression, we have also analyzed an experimental model of cachexia (Walker 256 carcinosarcoma). Animals were divided into: control, intermediate cachexia (IC) and terminal cachexia (TC). Subcutaneous white adipose tissue of patients and epidydimal white adipose tissue of animals were investigated regarding molecular aspects by determining the protein content and gene expression of hormone-sensitive lipase (HSL), adipose triglyceride lipase (ATGL), comparative gene identification-58 (CGI-58), perilipin 1, leptin, adiponectin, visfatin, and tumour necrosis factor alpha (TNF-alpha). Results: We found augmented lipolysis in CC associated with increased HSL expression, as well as upregulation of ATGL expression and reduction in perilipin 1 content. In IC, there was an imbalance in the secretion of pro-and anti-inflammatory factors. The alterations at the end-stage of cachexia were even more profound, and there was a reduction in the expression of almost all proteins analyzed in the animals. Conclusions: Our findings show that cachexia induces important morphological, molecular, and humoral alterations in the white adipose tissue, which are specific to the stage of the syndrome. |
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Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancerBackground: Cancer cachexia is a multifactorial metabolic syndrome characterized by marked loss of adipose tissue and skeletal muscle. Fat loss from adipose tissue in cancer cachexia is partly the result of increased lipolysis. Despite the growing amount of studies focused on elucidating the mechanisms through which lipolysis-related proteins regulate the lipolytic process, there are scarce data concerning that profile in the adipose tissue of cancer cachectic patients. Considering its fundamental importance, it was our main purpose to characterize the expression of the lipolysis-related proteins in the white adipose tissue of cachectic cancer patients. Methods: Patients from the University Hospital were divided into three groups: control, cancer cachexia (CC), and weight-stable cancer patients (WSC). To gain greater insight into adipose tissue wasting during cancer cachexia progression, we have also analyzed an experimental model of cachexia (Walker 256 carcinosarcoma). Animals were divided into: control, intermediate cachexia (IC) and terminal cachexia (TC). Subcutaneous white adipose tissue of patients and epidydimal white adipose tissue of animals were investigated regarding molecular aspects by determining the protein content and gene expression of hormone-sensitive lipase (HSL), adipose triglyceride lipase (ATGL), comparative gene identification-58 (CGI-58), perilipin 1, leptin, adiponectin, visfatin, and tumour necrosis factor alpha (TNF-alpha). Results: We found augmented lipolysis in CC associated with increased HSL expression, as well as upregulation of ATGL expression and reduction in perilipin 1 content. In IC, there was an imbalance in the secretion of pro-and anti-inflammatory factors. The alterations at the end-stage of cachexia were even more profound, and there was a reduction in the expression of almost all proteins analyzed in the animals. Conclusions: Our findings show that cachexia induces important morphological, molecular, and humoral alterations in the white adipose tissue, which are specific to the stage of the syndrome.Univ São Paulo, Canc Metab Res Grp, Inst Biomed Sci, Dept Surg,Fac Med, São Paulo, BrazilSão Paulo State Univ UNESP, Exercise & Immunometab Res Grp, Dept Phys Educ, Presidente Prudente, SP, BrazilUniv Fed São Paulo, UNIFESP, Dept Fisiol, São Paulo, BrazilUniv São Paulo, Univ Hosp, Dept Clin Surg, São Paulo, BrazilUniv Mogi das Cruzes, Lab Adipose Tissue Biol, Ctr Integrated Biotechnol, Mogi Das Cruzes, BrazilUniv São Paulo, Inst Biomed Sci, Ave Prof Lineu Prestes 1524,Lab 434, BR-05508900 São Paulo, SP, BrazilUniv Fed São Paulo, UNIFESP, Dept Fisiol, São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)FAPESP: 2012/50079-0Biomed Central Ltd2019-08-19T11:49:42Z2019-08-19T11:49:42Z2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion-application/pdfhttps://dx.doi.org/10.1186/s12944-017-0547-xLipids In Health And Disease. London, v. 16, p. -, 2017.10.1186/s12944-017-0547-xWOS000408372700002.pdf1476-511Xhttps://repositorio.unifesp.br/handle/11600/51379WOS:000408372700002enginfo:eu-repo/semantics/openAccessSilverio, RenataLira, Fabio Santos deOyama, Lila Missae [UNIFESP]Nascimento, Claudia Maria da Penha Oller do [UNIFESP]Otoch, Jose PinhataAlcantara, Paulo S. M.Batista Junior, Miguel LuizSeelaender, Mariliareponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-03T01:45:52Zoai:repositorio.unifesp.br/:11600/51379Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-03T01:45:52Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancer |
title |
Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancer |
spellingShingle |
Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancer Silverio, Renata |
title_short |
Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancer |
title_full |
Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancer |
title_fullStr |
Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancer |
title_full_unstemmed |
Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancer |
title_sort |
Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancer |
author |
Silverio, Renata |
author_facet |
Silverio, Renata Lira, Fabio Santos de Oyama, Lila Missae [UNIFESP] Nascimento, Claudia Maria da Penha Oller do [UNIFESP] Otoch, Jose Pinhata Alcantara, Paulo S. M. Batista Junior, Miguel Luiz Seelaender, Marilia |
author_role |
author |
author2 |
Lira, Fabio Santos de Oyama, Lila Missae [UNIFESP] Nascimento, Claudia Maria da Penha Oller do [UNIFESP] Otoch, Jose Pinhata Alcantara, Paulo S. M. Batista Junior, Miguel Luiz Seelaender, Marilia |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Silverio, Renata Lira, Fabio Santos de Oyama, Lila Missae [UNIFESP] Nascimento, Claudia Maria da Penha Oller do [UNIFESP] Otoch, Jose Pinhata Alcantara, Paulo S. M. Batista Junior, Miguel Luiz Seelaender, Marilia |
description |
Background: Cancer cachexia is a multifactorial metabolic syndrome characterized by marked loss of adipose tissue and skeletal muscle. Fat loss from adipose tissue in cancer cachexia is partly the result of increased lipolysis. Despite the growing amount of studies focused on elucidating the mechanisms through which lipolysis-related proteins regulate the lipolytic process, there are scarce data concerning that profile in the adipose tissue of cancer cachectic patients. Considering its fundamental importance, it was our main purpose to characterize the expression of the lipolysis-related proteins in the white adipose tissue of cachectic cancer patients. Methods: Patients from the University Hospital were divided into three groups: control, cancer cachexia (CC), and weight-stable cancer patients (WSC). To gain greater insight into adipose tissue wasting during cancer cachexia progression, we have also analyzed an experimental model of cachexia (Walker 256 carcinosarcoma). Animals were divided into: control, intermediate cachexia (IC) and terminal cachexia (TC). Subcutaneous white adipose tissue of patients and epidydimal white adipose tissue of animals were investigated regarding molecular aspects by determining the protein content and gene expression of hormone-sensitive lipase (HSL), adipose triglyceride lipase (ATGL), comparative gene identification-58 (CGI-58), perilipin 1, leptin, adiponectin, visfatin, and tumour necrosis factor alpha (TNF-alpha). Results: We found augmented lipolysis in CC associated with increased HSL expression, as well as upregulation of ATGL expression and reduction in perilipin 1 content. In IC, there was an imbalance in the secretion of pro-and anti-inflammatory factors. The alterations at the end-stage of cachexia were even more profound, and there was a reduction in the expression of almost all proteins analyzed in the animals. Conclusions: Our findings show that cachexia induces important morphological, molecular, and humoral alterations in the white adipose tissue, which are specific to the stage of the syndrome. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 2019-08-19T11:49:42Z 2019-08-19T11:49:42Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://dx.doi.org/10.1186/s12944-017-0547-x Lipids In Health And Disease. London, v. 16, p. -, 2017. 10.1186/s12944-017-0547-x WOS000408372700002.pdf 1476-511X https://repositorio.unifesp.br/handle/11600/51379 WOS:000408372700002 |
url |
https://dx.doi.org/10.1186/s12944-017-0547-x https://repositorio.unifesp.br/handle/11600/51379 |
identifier_str_mv |
Lipids In Health And Disease. London, v. 16, p. -, 2017. 10.1186/s12944-017-0547-x WOS000408372700002.pdf 1476-511X WOS:000408372700002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
- application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd |
publisher.none.fl_str_mv |
Biomed Central Ltd |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268348538552320 |