Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancer

Detalhes bibliográficos
Autor(a) principal: Silverio, Renata
Data de Publicação: 2017
Outros Autores: Lira, Fabio Santos de, Oyama, Lila Missae [UNIFESP], Nascimento, Claudia Maria da Penha Oller do [UNIFESP], Otoch, Jose Pinhata, Alcantara, Paulo S. M., Batista Junior, Miguel Luiz, Seelaender, Marilia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: https://dx.doi.org/10.1186/s12944-017-0547-x
https://repositorio.unifesp.br/handle/11600/51379
Resumo: Background: Cancer cachexia is a multifactorial metabolic syndrome characterized by marked loss of adipose tissue and skeletal muscle. Fat loss from adipose tissue in cancer cachexia is partly the result of increased lipolysis. Despite the growing amount of studies focused on elucidating the mechanisms through which lipolysis-related proteins regulate the lipolytic process, there are scarce data concerning that profile in the adipose tissue of cancer cachectic patients. Considering its fundamental importance, it was our main purpose to characterize the expression of the lipolysis-related proteins in the white adipose tissue of cachectic cancer patients. Methods: Patients from the University Hospital were divided into three groups: control, cancer cachexia (CC), and weight-stable cancer patients (WSC). To gain greater insight into adipose tissue wasting during cancer cachexia progression, we have also analyzed an experimental model of cachexia (Walker 256 carcinosarcoma). Animals were divided into: control, intermediate cachexia (IC) and terminal cachexia (TC). Subcutaneous white adipose tissue of patients and epidydimal white adipose tissue of animals were investigated regarding molecular aspects by determining the protein content and gene expression of hormone-sensitive lipase (HSL), adipose triglyceride lipase (ATGL), comparative gene identification-58 (CGI-58), perilipin 1, leptin, adiponectin, visfatin, and tumour necrosis factor alpha (TNF-alpha). Results: We found augmented lipolysis in CC associated with increased HSL expression, as well as upregulation of ATGL expression and reduction in perilipin 1 content. In IC, there was an imbalance in the secretion of pro-and anti-inflammatory factors. The alterations at the end-stage of cachexia were even more profound, and there was a reduction in the expression of almost all proteins analyzed in the animals. Conclusions: Our findings show that cachexia induces important morphological, molecular, and humoral alterations in the white adipose tissue, which are specific to the stage of the syndrome.
id UFSP_c6a6f25f431e06d4a8c214e70bf4aa16
oai_identifier_str oai:repositorio.unifesp.br/:11600/51379
network_acronym_str UFSP
network_name_str Repositório Institucional da UNIFESP
repository_id_str 3465
spelling Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancerBackground: Cancer cachexia is a multifactorial metabolic syndrome characterized by marked loss of adipose tissue and skeletal muscle. Fat loss from adipose tissue in cancer cachexia is partly the result of increased lipolysis. Despite the growing amount of studies focused on elucidating the mechanisms through which lipolysis-related proteins regulate the lipolytic process, there are scarce data concerning that profile in the adipose tissue of cancer cachectic patients. Considering its fundamental importance, it was our main purpose to characterize the expression of the lipolysis-related proteins in the white adipose tissue of cachectic cancer patients. Methods: Patients from the University Hospital were divided into three groups: control, cancer cachexia (CC), and weight-stable cancer patients (WSC). To gain greater insight into adipose tissue wasting during cancer cachexia progression, we have also analyzed an experimental model of cachexia (Walker 256 carcinosarcoma). Animals were divided into: control, intermediate cachexia (IC) and terminal cachexia (TC). Subcutaneous white adipose tissue of patients and epidydimal white adipose tissue of animals were investigated regarding molecular aspects by determining the protein content and gene expression of hormone-sensitive lipase (HSL), adipose triglyceride lipase (ATGL), comparative gene identification-58 (CGI-58), perilipin 1, leptin, adiponectin, visfatin, and tumour necrosis factor alpha (TNF-alpha). Results: We found augmented lipolysis in CC associated with increased HSL expression, as well as upregulation of ATGL expression and reduction in perilipin 1 content. In IC, there was an imbalance in the secretion of pro-and anti-inflammatory factors. The alterations at the end-stage of cachexia were even more profound, and there was a reduction in the expression of almost all proteins analyzed in the animals. Conclusions: Our findings show that cachexia induces important morphological, molecular, and humoral alterations in the white adipose tissue, which are specific to the stage of the syndrome.Univ São Paulo, Canc Metab Res Grp, Inst Biomed Sci, Dept Surg,Fac Med, São Paulo, BrazilSão Paulo State Univ UNESP, Exercise & Immunometab Res Grp, Dept Phys Educ, Presidente Prudente, SP, BrazilUniv Fed São Paulo, UNIFESP, Dept Fisiol, São Paulo, BrazilUniv São Paulo, Univ Hosp, Dept Clin Surg, São Paulo, BrazilUniv Mogi das Cruzes, Lab Adipose Tissue Biol, Ctr Integrated Biotechnol, Mogi Das Cruzes, BrazilUniv São Paulo, Inst Biomed Sci, Ave Prof Lineu Prestes 1524,Lab 434, BR-05508900 São Paulo, SP, BrazilUniv Fed São Paulo, UNIFESP, Dept Fisiol, São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)FAPESP: 2012/50079-0Biomed Central Ltd2019-08-19T11:49:42Z2019-08-19T11:49:42Z2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion-application/pdfhttps://dx.doi.org/10.1186/s12944-017-0547-xLipids In Health And Disease. London, v. 16, p. -, 2017.10.1186/s12944-017-0547-xWOS000408372700002.pdf1476-511Xhttps://repositorio.unifesp.br/handle/11600/51379WOS:000408372700002enginfo:eu-repo/semantics/openAccessSilverio, RenataLira, Fabio Santos deOyama, Lila Missae [UNIFESP]Nascimento, Claudia Maria da Penha Oller do [UNIFESP]Otoch, Jose PinhataAlcantara, Paulo S. M.Batista Junior, Miguel LuizSeelaender, Mariliareponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-03T01:45:52Zoai:repositorio.unifesp.br/:11600/51379Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-03T01:45:52Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancer
title Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancer
spellingShingle Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancer
Silverio, Renata
title_short Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancer
title_full Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancer
title_fullStr Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancer
title_full_unstemmed Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancer
title_sort Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancer
author Silverio, Renata
author_facet Silverio, Renata
Lira, Fabio Santos de
Oyama, Lila Missae [UNIFESP]
Nascimento, Claudia Maria da Penha Oller do [UNIFESP]
Otoch, Jose Pinhata
Alcantara, Paulo S. M.
Batista Junior, Miguel Luiz
Seelaender, Marilia
author_role author
author2 Lira, Fabio Santos de
Oyama, Lila Missae [UNIFESP]
Nascimento, Claudia Maria da Penha Oller do [UNIFESP]
Otoch, Jose Pinhata
Alcantara, Paulo S. M.
Batista Junior, Miguel Luiz
Seelaender, Marilia
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silverio, Renata
Lira, Fabio Santos de
Oyama, Lila Missae [UNIFESP]
Nascimento, Claudia Maria da Penha Oller do [UNIFESP]
Otoch, Jose Pinhata
Alcantara, Paulo S. M.
Batista Junior, Miguel Luiz
Seelaender, Marilia
description Background: Cancer cachexia is a multifactorial metabolic syndrome characterized by marked loss of adipose tissue and skeletal muscle. Fat loss from adipose tissue in cancer cachexia is partly the result of increased lipolysis. Despite the growing amount of studies focused on elucidating the mechanisms through which lipolysis-related proteins regulate the lipolytic process, there are scarce data concerning that profile in the adipose tissue of cancer cachectic patients. Considering its fundamental importance, it was our main purpose to characterize the expression of the lipolysis-related proteins in the white adipose tissue of cachectic cancer patients. Methods: Patients from the University Hospital were divided into three groups: control, cancer cachexia (CC), and weight-stable cancer patients (WSC). To gain greater insight into adipose tissue wasting during cancer cachexia progression, we have also analyzed an experimental model of cachexia (Walker 256 carcinosarcoma). Animals were divided into: control, intermediate cachexia (IC) and terminal cachexia (TC). Subcutaneous white adipose tissue of patients and epidydimal white adipose tissue of animals were investigated regarding molecular aspects by determining the protein content and gene expression of hormone-sensitive lipase (HSL), adipose triglyceride lipase (ATGL), comparative gene identification-58 (CGI-58), perilipin 1, leptin, adiponectin, visfatin, and tumour necrosis factor alpha (TNF-alpha). Results: We found augmented lipolysis in CC associated with increased HSL expression, as well as upregulation of ATGL expression and reduction in perilipin 1 content. In IC, there was an imbalance in the secretion of pro-and anti-inflammatory factors. The alterations at the end-stage of cachexia were even more profound, and there was a reduction in the expression of almost all proteins analyzed in the animals. Conclusions: Our findings show that cachexia induces important morphological, molecular, and humoral alterations in the white adipose tissue, which are specific to the stage of the syndrome.
publishDate 2017
dc.date.none.fl_str_mv 2017
2019-08-19T11:49:42Z
2019-08-19T11:49:42Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://dx.doi.org/10.1186/s12944-017-0547-x
Lipids In Health And Disease. London, v. 16, p. -, 2017.
10.1186/s12944-017-0547-x
WOS000408372700002.pdf
1476-511X
https://repositorio.unifesp.br/handle/11600/51379
WOS:000408372700002
url https://dx.doi.org/10.1186/s12944-017-0547-x
https://repositorio.unifesp.br/handle/11600/51379
identifier_str_mv Lipids In Health And Disease. London, v. 16, p. -, 2017.
10.1186/s12944-017-0547-x
WOS000408372700002.pdf
1476-511X
WOS:000408372700002
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv -
application/pdf
dc.publisher.none.fl_str_mv Biomed Central Ltd
publisher.none.fl_str_mv Biomed Central Ltd
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268348538552320