Sleep Loss Induces Differential Response Related To Genotoxicity in Multiple Organs of Three Different Mice Strains
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/32647 http://dx.doi.org/10.1111/j.1742-7843.2010.00540.x |
Resumo: | The purpose of the present study was to determine the genetic damage induced by paradoxical sleep deprivation (PSD) in three different male mice strains in peripheral blood, heart, kidney and liver tissues by the single cell gel (comet) assay. Swiss, C57BL/6j and hairless (HRS/j) mice were submitted to PSD by the multiple platform technique for 72 hr, and DNA damage was evaluated. Statistically significant differences in DNA damage were found in blood cells of the Swiss mice strain when compared to negative controls. By contrast, no statistically significant differences were found in the C57BL/6j or hairless mice strains. With regard to the liver, extensive genotoxic effects were found in the Swiss strain. the hairless and C57BL/6j mice strains did not show any signs of genotoxocity in this organ. the same lack of effect was noted in kidney and heart cells of all strains evaluated. in conclusion, our results reveal that sleep deprivation exerted genetic damage in the form of DNA breakage in blood and liver cells of the Swiss mice strain only. This type of approach should be considered when studying noxious activities on genetic apparatus induced by sleep deprivation in mice since the Swiss strain is more suitable for this purpose. |
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Kahan, Vanessa [UNIFESP]Ribeiro, Daniel Araki [UNIFESP]Andersen, Monica Levy [UNIFESP]Alvarenga, Tathiana Aparecida [UNIFESP]Tufik, Sergio [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2016-01-24T13:59:49Z2016-01-24T13:59:49Z2010-07-01Basic & Clinical Pharmacology & Toxicology. Malden: Wiley-Blackwell, v. 107, n. 1, p. 598-602, 2010.1742-7835http://repositorio.unifesp.br/handle/11600/32647http://dx.doi.org/10.1111/j.1742-7843.2010.00540.x10.1111/j.1742-7843.2010.00540.xWOS:000278931000008The purpose of the present study was to determine the genetic damage induced by paradoxical sleep deprivation (PSD) in three different male mice strains in peripheral blood, heart, kidney and liver tissues by the single cell gel (comet) assay. Swiss, C57BL/6j and hairless (HRS/j) mice were submitted to PSD by the multiple platform technique for 72 hr, and DNA damage was evaluated. Statistically significant differences in DNA damage were found in blood cells of the Swiss mice strain when compared to negative controls. By contrast, no statistically significant differences were found in the C57BL/6j or hairless mice strains. With regard to the liver, extensive genotoxic effects were found in the Swiss strain. the hairless and C57BL/6j mice strains did not show any signs of genotoxocity in this organ. the same lack of effect was noted in kidney and heart cells of all strains evaluated. in conclusion, our results reveal that sleep deprivation exerted genetic damage in the form of DNA breakage in blood and liver cells of the Swiss mice strain only. This type of approach should be considered when studying noxious activities on genetic apparatus induced by sleep deprivation in mice since the Swiss strain is more suitable for this purpose.Associacao Fundo de Incentivo a Psicofarmacologia (AFIP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Federal de São Paulo, Dept Psychobiol, BR-04024002 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biosci, BR-04024002 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Psychobiol, BR-04024002 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biosci, BR-04024002 São Paulo, BrazilFAPESP: 06/58274-5FAPESP: 07/01228-4FAPESP: CEPID 98/14303-3Web of Science598-602engWiley-BlackwellBasic & Clinical Pharmacology & Toxicologyhttp://olabout.wiley.com/WileyCDA/Section/id-406071.htmlinfo:eu-repo/semantics/openAccessSleep Loss Induces Differential Response Related To Genotoxicity in Multiple Organs of Three Different Mice Strainsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/326472022-09-27 09:38:18.73metadata only accessoai:repositorio.unifesp.br:11600/32647Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-09-27T12:38:18Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Sleep Loss Induces Differential Response Related To Genotoxicity in Multiple Organs of Three Different Mice Strains |
title |
Sleep Loss Induces Differential Response Related To Genotoxicity in Multiple Organs of Three Different Mice Strains |
spellingShingle |
Sleep Loss Induces Differential Response Related To Genotoxicity in Multiple Organs of Three Different Mice Strains Kahan, Vanessa [UNIFESP] |
title_short |
Sleep Loss Induces Differential Response Related To Genotoxicity in Multiple Organs of Three Different Mice Strains |
title_full |
Sleep Loss Induces Differential Response Related To Genotoxicity in Multiple Organs of Three Different Mice Strains |
title_fullStr |
Sleep Loss Induces Differential Response Related To Genotoxicity in Multiple Organs of Three Different Mice Strains |
title_full_unstemmed |
Sleep Loss Induces Differential Response Related To Genotoxicity in Multiple Organs of Three Different Mice Strains |
title_sort |
Sleep Loss Induces Differential Response Related To Genotoxicity in Multiple Organs of Three Different Mice Strains |
author |
Kahan, Vanessa [UNIFESP] |
author_facet |
Kahan, Vanessa [UNIFESP] Ribeiro, Daniel Araki [UNIFESP] Andersen, Monica Levy [UNIFESP] Alvarenga, Tathiana Aparecida [UNIFESP] Tufik, Sergio [UNIFESP] |
author_role |
author |
author2 |
Ribeiro, Daniel Araki [UNIFESP] Andersen, Monica Levy [UNIFESP] Alvarenga, Tathiana Aparecida [UNIFESP] Tufik, Sergio [UNIFESP] |
author2_role |
author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Kahan, Vanessa [UNIFESP] Ribeiro, Daniel Araki [UNIFESP] Andersen, Monica Levy [UNIFESP] Alvarenga, Tathiana Aparecida [UNIFESP] Tufik, Sergio [UNIFESP] |
description |
The purpose of the present study was to determine the genetic damage induced by paradoxical sleep deprivation (PSD) in three different male mice strains in peripheral blood, heart, kidney and liver tissues by the single cell gel (comet) assay. Swiss, C57BL/6j and hairless (HRS/j) mice were submitted to PSD by the multiple platform technique for 72 hr, and DNA damage was evaluated. Statistically significant differences in DNA damage were found in blood cells of the Swiss mice strain when compared to negative controls. By contrast, no statistically significant differences were found in the C57BL/6j or hairless mice strains. With regard to the liver, extensive genotoxic effects were found in the Swiss strain. the hairless and C57BL/6j mice strains did not show any signs of genotoxocity in this organ. the same lack of effect was noted in kidney and heart cells of all strains evaluated. in conclusion, our results reveal that sleep deprivation exerted genetic damage in the form of DNA breakage in blood and liver cells of the Swiss mice strain only. This type of approach should be considered when studying noxious activities on genetic apparatus induced by sleep deprivation in mice since the Swiss strain is more suitable for this purpose. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-07-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T13:59:49Z |
dc.date.available.fl_str_mv |
2016-01-24T13:59:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Basic & Clinical Pharmacology & Toxicology. Malden: Wiley-Blackwell, v. 107, n. 1, p. 598-602, 2010. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/32647 http://dx.doi.org/10.1111/j.1742-7843.2010.00540.x |
dc.identifier.issn.none.fl_str_mv |
1742-7835 |
dc.identifier.doi.none.fl_str_mv |
10.1111/j.1742-7843.2010.00540.x |
dc.identifier.wos.none.fl_str_mv |
WOS:000278931000008 |
identifier_str_mv |
Basic & Clinical Pharmacology & Toxicology. Malden: Wiley-Blackwell, v. 107, n. 1, p. 598-602, 2010. 1742-7835 10.1111/j.1742-7843.2010.00540.x WOS:000278931000008 |
url |
http://repositorio.unifesp.br/handle/11600/32647 http://dx.doi.org/10.1111/j.1742-7843.2010.00540.x |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Basic & Clinical Pharmacology & Toxicology |
dc.rights.driver.fl_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
598-602 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764162541027328 |