Evaluation of the changes on hemostatic parameters induced by valdecoxib in male Wistar rats
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2006 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| Texto Completo: | http://dx.doi.org/10.1590/S1516-84842006000100008 http://repositorio.unifesp.br/handle/11600/2946 |
Resumo: | The effects of the cyclooxygenase (COX)-2 selective inhibitor, valdecoxib, on blood coagulation parameters were evaluated, along with aspirin in male Wistar rats. Groups of animals were administered a daily oral dose of 10 mg/kg rat of valdecoxib, 100 mg/kg rat of aspirin and the vehicle alone during 4 weeks. Blood samples were collected at the end of 1, 2, 3 and 4 weeks of administration period and the plasma concentrations of valdecoxib were determined by RP-HPLC giving mean values of 101.1, 113.5, 164.0 and 184.6 ng/mL, respectively. The same plasma samples were used for the analysis of hematological parameters and the results compared to the controls. Valdecoxib induced significant activated partial thromboplastin time reduction (18%) after 2 weeks and prothrombin time reduction (12.2%) after 3 weeks (P<0.05). There were no significant changes in the platelet count and fibrinogen levels. The anti-factor Xa and anti-factor IIa activities showed slight reductions, but only significant for the anti-factor Xa on the 3rd week (6.7%). The results showed that valdecoxib at the dose tested with the plasmatic concentrations induced some changes in the hemostatic function of rats, which can be helpful to understand the side effects and the safe use of the drug. |
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Evaluation of the changes on hemostatic parameters induced by valdecoxib in male Wistar ratsAvaliação dos efeitos do valdecoxibe sobre os parâmetros hemostáticos de ratos WistarValdecoxibcoagulationliquid chromatographyratsfactor XaValdecoxibecoagulaçãocromatografia líquidaratosfator XaThe effects of the cyclooxygenase (COX)-2 selective inhibitor, valdecoxib, on blood coagulation parameters were evaluated, along with aspirin in male Wistar rats. Groups of animals were administered a daily oral dose of 10 mg/kg rat of valdecoxib, 100 mg/kg rat of aspirin and the vehicle alone during 4 weeks. Blood samples were collected at the end of 1, 2, 3 and 4 weeks of administration period and the plasma concentrations of valdecoxib were determined by RP-HPLC giving mean values of 101.1, 113.5, 164.0 and 184.6 ng/mL, respectively. The same plasma samples were used for the analysis of hematological parameters and the results compared to the controls. Valdecoxib induced significant activated partial thromboplastin time reduction (18%) after 2 weeks and prothrombin time reduction (12.2%) after 3 weeks (P<0.05). There were no significant changes in the platelet count and fibrinogen levels. The anti-factor Xa and anti-factor IIa activities showed slight reductions, but only significant for the anti-factor Xa on the 3rd week (6.7%). The results showed that valdecoxib at the dose tested with the plasmatic concentrations induced some changes in the hemostatic function of rats, which can be helpful to understand the side effects and the safe use of the drug.Avaliaram-se os efeitos do valdecoxibe, um inibidor seletivo da cicloxigenase-2 (COX-2), sobre os parâ­metros sangüíneos da coagulação em ratos Wistar utilizando-se paralelamente a aspirina. Os animais foram divididos em grupos e submetidos à administração oral diária de 10 mg/kg animal para o valdecoxib, 100 mg/kg animal para a aspirina e veículo para o grupo controle, durante quatro semanas. A coleta do sangue foi efetuada após uma, duas, três e quatro semanas, e as concentrações plasmáticas do valdecoxibe determinadas por cromatografia líquida, obtendo-se valores médios de 101,1 - 113,5 - 164 e 184,6 ng/ml, respectivamente. As amostras de plasma foram também usadas para as análises hematológicas e os resultados comparados aos dos controles. O valde­coxibe apresentou redução significativa no TTPA (18%) após duas semanas, e redução do TP (12,2%) após três semanas (P<0.05). Os efeitos observados na contagem de pla­quetas e nos níveis plasmáticos de fibrinogênio não foram significativos. As atividades anti-fator Xa e anti-fator IIa apresentaram redução, porém os resultados foram significativos somente para o anti-fator Xa na terceira semana (6,7%). Os resultados experimentais com as concentrações plasmáticas alcan­çadas demonstram que o valdecoxibe pode induzir alterações nos parâ­metros hematológicos dos ratos. Esses dados poderão contribuir para a melhor compreensão dos efeitos colaterais e uso do fármaco com segurança.Industrial PharmacyUNIFESPUNIFESPSciELOCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Associação Brasileira de Hematologia e Hemoterapia e Terapia CelularIndustrial PharmacyUniversidade Federal de São Paulo (UNIFESP)Fronza, MarcioWrasse, MicheliBrum Junior, LiberatoSangoi, Maximiliano S.Dalmora, Sérgio L. [UNIFESP]2015-06-14T13:32:00Z2015-06-14T13:32:00Z2006-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion28-32application/pdfhttp://dx.doi.org/10.1590/S1516-84842006000100008Revista Brasileira de Hematologia e Hemoterapia. Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular, v. 28, n. 1, p. 28-32, 2006.10.1590/S1516-84842006000100008S1516-84842006000100008.pdf1516-8484S1516-84842006000100008http://repositorio.unifesp.br/handle/11600/2946engRevista Brasileira de Hematologia e Hemoterapiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-28T10:18:38Zoai:repositorio.unifesp.br/:11600/2946Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-28T10:18:38Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
Evaluation of the changes on hemostatic parameters induced by valdecoxib in male Wistar rats Avaliação dos efeitos do valdecoxibe sobre os parâmetros hemostáticos de ratos Wistar |
| title |
Evaluation of the changes on hemostatic parameters induced by valdecoxib in male Wistar rats |
| spellingShingle |
Evaluation of the changes on hemostatic parameters induced by valdecoxib in male Wistar rats Fronza, Marcio Valdecoxib coagulation liquid chromatography rats factor Xa Valdecoxibe coagulação cromatografia líquida ratos fator Xa |
| title_short |
Evaluation of the changes on hemostatic parameters induced by valdecoxib in male Wistar rats |
| title_full |
Evaluation of the changes on hemostatic parameters induced by valdecoxib in male Wistar rats |
| title_fullStr |
Evaluation of the changes on hemostatic parameters induced by valdecoxib in male Wistar rats |
| title_full_unstemmed |
Evaluation of the changes on hemostatic parameters induced by valdecoxib in male Wistar rats |
| title_sort |
Evaluation of the changes on hemostatic parameters induced by valdecoxib in male Wistar rats |
| author |
Fronza, Marcio |
| author_facet |
Fronza, Marcio Wrasse, Micheli Brum Junior, Liberato Sangoi, Maximiliano S. Dalmora, Sérgio L. [UNIFESP] |
| author_role |
author |
| author2 |
Wrasse, Micheli Brum Junior, Liberato Sangoi, Maximiliano S. Dalmora, Sérgio L. [UNIFESP] |
| author2_role |
author author author author |
| dc.contributor.none.fl_str_mv |
Industrial Pharmacy Universidade Federal de São Paulo (UNIFESP) |
| dc.contributor.author.fl_str_mv |
Fronza, Marcio Wrasse, Micheli Brum Junior, Liberato Sangoi, Maximiliano S. Dalmora, Sérgio L. [UNIFESP] |
| dc.subject.por.fl_str_mv |
Valdecoxib coagulation liquid chromatography rats factor Xa Valdecoxibe coagulação cromatografia líquida ratos fator Xa |
| topic |
Valdecoxib coagulation liquid chromatography rats factor Xa Valdecoxibe coagulação cromatografia líquida ratos fator Xa |
| description |
The effects of the cyclooxygenase (COX)-2 selective inhibitor, valdecoxib, on blood coagulation parameters were evaluated, along with aspirin in male Wistar rats. Groups of animals were administered a daily oral dose of 10 mg/kg rat of valdecoxib, 100 mg/kg rat of aspirin and the vehicle alone during 4 weeks. Blood samples were collected at the end of 1, 2, 3 and 4 weeks of administration period and the plasma concentrations of valdecoxib were determined by RP-HPLC giving mean values of 101.1, 113.5, 164.0 and 184.6 ng/mL, respectively. The same plasma samples were used for the analysis of hematological parameters and the results compared to the controls. Valdecoxib induced significant activated partial thromboplastin time reduction (18%) after 2 weeks and prothrombin time reduction (12.2%) after 3 weeks (P<0.05). There were no significant changes in the platelet count and fibrinogen levels. The anti-factor Xa and anti-factor IIa activities showed slight reductions, but only significant for the anti-factor Xa on the 3rd week (6.7%). The results showed that valdecoxib at the dose tested with the plasmatic concentrations induced some changes in the hemostatic function of rats, which can be helpful to understand the side effects and the safe use of the drug. |
| publishDate |
2006 |
| dc.date.none.fl_str_mv |
2006-03-01 2015-06-14T13:32:00Z 2015-06-14T13:32:00Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1516-84842006000100008 Revista Brasileira de Hematologia e Hemoterapia. Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular, v. 28, n. 1, p. 28-32, 2006. 10.1590/S1516-84842006000100008 S1516-84842006000100008.pdf 1516-8484 S1516-84842006000100008 http://repositorio.unifesp.br/handle/11600/2946 |
| url |
http://dx.doi.org/10.1590/S1516-84842006000100008 http://repositorio.unifesp.br/handle/11600/2946 |
| identifier_str_mv |
Revista Brasileira de Hematologia e Hemoterapia. Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular, v. 28, n. 1, p. 28-32, 2006. 10.1590/S1516-84842006000100008 S1516-84842006000100008.pdf 1516-8484 S1516-84842006000100008 |
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eng |
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eng |
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Revista Brasileira de Hematologia e Hemoterapia |
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info:eu-repo/semantics/openAccess |
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openAccess |
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28-32 application/pdf |
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Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular |
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Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular |
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reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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Universidade Federal de São Paulo (UNIFESP) |
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UNIFESP |
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UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
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biblioteca.csp@unifesp.br |
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1814268367693938688 |