ZAP-70: aspectos práticos

Detalhes bibliográficos
Autor(a) principal: Yamamoto, Mihoko [UNIFESP]
Data de Publicação: 2005
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S1516-84842005000400004
http://repositorio.unifesp.br/handle/11600/2835
Resumo: ZAP-70 is a 70Kd protein kinase present in normal T Lymphocytes and natural killer cells (NK), but absent in normal B lymphocytes. Nevertheless, B cells in some B-cell Chronic Lymphocytic Leukemia (CLL) patients express ZAP-70 and it has been found to be associated with the unmutated status of immunoglobulin heavy chains (IgVH) genes, a strong prognostic factor in CLL. In addition, the ZAP-70 expression in CLL cells is stable over time, differently to the CD38 expression, another prognostic marker in CLL. Several studies have been performed to validate the ZAP-70 expression as a surrogate marker for the IgVH mutation status which uses a laborious and time consuming technique. Using monoclonal antibodies, anti-ZAP-70 is easily detected by a rapid and sensitive flow cytometric technique. Upstate Biotechnology developed the first unconjugated antibody (2F3.2 clone) and soon after other products were developed, including fluorochrome conjugated ones, facilitating the technical assays. ZAP-70 can be evaluated by flow cytometric methods and its expression predicts the prognosis in the great majority of CLL cases.
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spelling ZAP-70: aspectos práticosZAP-70: practical aspectsZAP-70CLLprognosisZAP-70LLCprognósticoZAP-70 is a 70Kd protein kinase present in normal T Lymphocytes and natural killer cells (NK), but absent in normal B lymphocytes. Nevertheless, B cells in some B-cell Chronic Lymphocytic Leukemia (CLL) patients express ZAP-70 and it has been found to be associated with the unmutated status of immunoglobulin heavy chains (IgVH) genes, a strong prognostic factor in CLL. In addition, the ZAP-70 expression in CLL cells is stable over time, differently to the CD38 expression, another prognostic marker in CLL. Several studies have been performed to validate the ZAP-70 expression as a surrogate marker for the IgVH mutation status which uses a laborious and time consuming technique. Using monoclonal antibodies, anti-ZAP-70 is easily detected by a rapid and sensitive flow cytometric technique. Upstate Biotechnology developed the first unconjugated antibody (2F3.2 clone) and soon after other products were developed, including fluorochrome conjugated ones, facilitating the technical assays. ZAP-70 can be evaluated by flow cytometric methods and its expression predicts the prognosis in the great majority of CLL cases.A ZAP-70 é uma proteína kinase normalmente expressa nos linfócitos T e células NK, mas ausente nos linfócitos B. No entanto, ela está expressa nas células B de pacientes com LLC que não apresentam mutação nos genes da região variável da cadeia pesada de imunoglobulina (genes IgVH). Além disso, observa-se que a expressão da ZAP-70 é estável nestes pacientes ao longo do tempo, diferentemente da expressão de CD38, outro marcador considerado de prognóstico nesta doença. Estudos têm sido realizados para validá-la como marcador surrogate para o estado de mutação dos genes IgVH (um forte fator de prognóstico), uma vez que a sua avaliação molecular é laboriosa e demorada. O método mais rápido e simples para avaliar a expressão da ZAP-70 é através da citometria de fluxo utilizando-se anticorpos monoclonais já disponíveis no mercado. O primeiro produto comercial (clone 2F3.2 da marca Upstate) é puro, não conjugado com fluorocromos, o que torna o ensaio laborioso, apesar de resultados consistentes. Rapidamente surgiram outros Ac conjugados com fluorocromos (com FITC da própria Upstate, ou Alexa-fluor, FITC ou PE da Caltag) e estudos estão sendo realizados para a sua validação técnica.Universidade Federal de São Paulo (UNIFESP)UNIFESP, EPM, São Paulo, BrazilSciELOAssociação Brasileira de Hematologia e Hemoterapia e Terapia CelularUniversidade Federal de São Paulo (UNIFESP)Yamamoto, Mihoko [UNIFESP]2015-06-14T13:31:53Z2015-06-14T13:31:53Z2005-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion236-240application/pdfhttp://dx.doi.org/10.1590/S1516-84842005000400004Revista Brasileira de Hematologia e Hemoterapia. Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular, v. 27, n. 4, p. 236-240, 2005.10.1590/S1516-84842005000400004S1516-84842005000400004.pdf1516-8484S1516-84842005000400004http://repositorio.unifesp.br/handle/11600/2835porRevista Brasileira de Hematologia e Hemoterapiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-28T09:45:22Zoai:repositorio.unifesp.br/:11600/2835Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-28T09:45:22Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv ZAP-70: aspectos práticos
ZAP-70: practical aspects
title ZAP-70: aspectos práticos
spellingShingle ZAP-70: aspectos práticos
Yamamoto, Mihoko [UNIFESP]
ZAP-70
CLL
prognosis
ZAP-70
LLC
prognóstico
title_short ZAP-70: aspectos práticos
title_full ZAP-70: aspectos práticos
title_fullStr ZAP-70: aspectos práticos
title_full_unstemmed ZAP-70: aspectos práticos
title_sort ZAP-70: aspectos práticos
author Yamamoto, Mihoko [UNIFESP]
author_facet Yamamoto, Mihoko [UNIFESP]
author_role author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Yamamoto, Mihoko [UNIFESP]
dc.subject.por.fl_str_mv ZAP-70
CLL
prognosis
ZAP-70
LLC
prognóstico
topic ZAP-70
CLL
prognosis
ZAP-70
LLC
prognóstico
description ZAP-70 is a 70Kd protein kinase present in normal T Lymphocytes and natural killer cells (NK), but absent in normal B lymphocytes. Nevertheless, B cells in some B-cell Chronic Lymphocytic Leukemia (CLL) patients express ZAP-70 and it has been found to be associated with the unmutated status of immunoglobulin heavy chains (IgVH) genes, a strong prognostic factor in CLL. In addition, the ZAP-70 expression in CLL cells is stable over time, differently to the CD38 expression, another prognostic marker in CLL. Several studies have been performed to validate the ZAP-70 expression as a surrogate marker for the IgVH mutation status which uses a laborious and time consuming technique. Using monoclonal antibodies, anti-ZAP-70 is easily detected by a rapid and sensitive flow cytometric technique. Upstate Biotechnology developed the first unconjugated antibody (2F3.2 clone) and soon after other products were developed, including fluorochrome conjugated ones, facilitating the technical assays. ZAP-70 can be evaluated by flow cytometric methods and its expression predicts the prognosis in the great majority of CLL cases.
publishDate 2005
dc.date.none.fl_str_mv 2005-12-01
2015-06-14T13:31:53Z
2015-06-14T13:31:53Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1516-84842005000400004
Revista Brasileira de Hematologia e Hemoterapia. Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular, v. 27, n. 4, p. 236-240, 2005.
10.1590/S1516-84842005000400004
S1516-84842005000400004.pdf
1516-8484
S1516-84842005000400004
http://repositorio.unifesp.br/handle/11600/2835
url http://dx.doi.org/10.1590/S1516-84842005000400004
http://repositorio.unifesp.br/handle/11600/2835
identifier_str_mv Revista Brasileira de Hematologia e Hemoterapia. Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular, v. 27, n. 4, p. 236-240, 2005.
10.1590/S1516-84842005000400004
S1516-84842005000400004.pdf
1516-8484
S1516-84842005000400004
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Revista Brasileira de Hematologia e Hemoterapia
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 236-240
application/pdf
dc.publisher.none.fl_str_mv Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular
publisher.none.fl_str_mv Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268329904308224

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