Adipose-derived stem cells (ADSC) in the viability of a random pattern dorsal skin flap in rats
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/8208 http://dx.doi.org/10.1590/S0102-86502014001700001 |
Resumo: | PURPOSE:To evaluate the viability of random pattern dorsal skin flaps in rats after injection of adipose-derived stem cells (ADSC).METHODS: Thirty five adult male Wistar EPM rats (weight 250-300 g) were distributed, at random, in two groups. I- Control (flap elevation with injection of saline solution) with fifteen animals and II- Experimental (flap elevation with injection of ADSC ) with fifteen animal. The ADSC were isolated from others five adult male rats. A dorsal skin flap measuring 10x4 cm was raised and a plastic barrier was placed between the flap and its bed in both groups and the injection (cells or saline solution) were perfomed immediately after the surgery. The percentage of flap necrosis was measured on the seventh postoperative day.RESULTS:The ADSC were able to replicate in our culture conditions. We also induced their adipogenic, osteogenic and chondrogenic differentiation to verify their mesenchymal stem cells potentiality in vitro. The results were statistically significant showing that the ADSC decreased the area of necrosis (p<0.05).CONCLUSIONS:The cells demonstrated adipogenic, osteogenic and chondrogenic differentiation potential in vitro. The administration of adipose-derived stem cells was effective to increase the viability of the random random pattern dorsal skin flaps in rats. |
id |
UFSP_cbe3a7c64ff76966611e55ec6ad22e62 |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br:11600/8208 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Suartz, Caio ViniciusGaiba, SilvanaFrança, Jerônimo Pereira deAloise, Antonio Carlos [UNIFESP]Ferreira, Lydia Masako [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Universidade Estadual de Santa Cruz Department of Surgery2015-06-14T13:46:54Z2015-06-14T13:46:54Z2014-01-01Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 02-05, 2014.0102-8650http://repositorio.unifesp.br/handle/11600/8208http://dx.doi.org/10.1590/S0102-86502014001700001S0102-86502014001500002.pdfS0102-8650201400150000210.1590/S0102-86502014001700001WOS:000344466300002PURPOSE:To evaluate the viability of random pattern dorsal skin flaps in rats after injection of adipose-derived stem cells (ADSC).METHODS: Thirty five adult male Wistar EPM rats (weight 250-300 g) were distributed, at random, in two groups. I- Control (flap elevation with injection of saline solution) with fifteen animals and II- Experimental (flap elevation with injection of ADSC ) with fifteen animal. The ADSC were isolated from others five adult male rats. A dorsal skin flap measuring 10x4 cm was raised and a plastic barrier was placed between the flap and its bed in both groups and the injection (cells or saline solution) were perfomed immediately after the surgery. The percentage of flap necrosis was measured on the seventh postoperative day.RESULTS:The ADSC were able to replicate in our culture conditions. We also induced their adipogenic, osteogenic and chondrogenic differentiation to verify their mesenchymal stem cells potentiality in vitro. The results were statistically significant showing that the ADSC decreased the area of necrosis (p<0.05).CONCLUSIONS:The cells demonstrated adipogenic, osteogenic and chondrogenic differentiation potential in vitro. The administration of adipose-derived stem cells was effective to increase the viability of the random random pattern dorsal skin flaps in rats.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Federal University of São Paulo Department of SurgeryUniversidade Estadual de Santa Cruz Department of SurgeryUNIFESPUNIFESP, Department of SurgeryUNIFESPCNPq: 312356/2009-9SciELO02-05engSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirurgica BrasileiraSurgical FlapsRatsAdult Stem CellsStem CellsAdipose TissueAdipose-derived stem cells (ADSC) in the viability of a random pattern dorsal skin flap in ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS0102-86502014001500002.pdfapplication/pdf400835${dspace.ui.url}/bitstream/11600/8208/1/S0102-86502014001500002.pdf4145a0cd99176ee031fd99c5f1756adbMD51open accessTEXTS0102-86502014001500002.pdf.txtS0102-86502014001500002.pdf.txtExtracted texttext/plain19273${dspace.ui.url}/bitstream/11600/8208/2/S0102-86502014001500002.pdf.txt3ea2f8b60f12f4a1243bd204af632f83MD52open access11600/82082021-09-30 11:01:51.29open accessoai:repositorio.unifesp.br:11600/8208Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:27:01.267298Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Adipose-derived stem cells (ADSC) in the viability of a random pattern dorsal skin flap in rats |
title |
Adipose-derived stem cells (ADSC) in the viability of a random pattern dorsal skin flap in rats |
spellingShingle |
Adipose-derived stem cells (ADSC) in the viability of a random pattern dorsal skin flap in rats Suartz, Caio Vinicius Surgical Flaps Rats Adult Stem Cells Stem Cells Adipose Tissue |
title_short |
Adipose-derived stem cells (ADSC) in the viability of a random pattern dorsal skin flap in rats |
title_full |
Adipose-derived stem cells (ADSC) in the viability of a random pattern dorsal skin flap in rats |
title_fullStr |
Adipose-derived stem cells (ADSC) in the viability of a random pattern dorsal skin flap in rats |
title_full_unstemmed |
Adipose-derived stem cells (ADSC) in the viability of a random pattern dorsal skin flap in rats |
title_sort |
Adipose-derived stem cells (ADSC) in the viability of a random pattern dorsal skin flap in rats |
author |
Suartz, Caio Vinicius |
author_facet |
Suartz, Caio Vinicius Gaiba, Silvana França, Jerônimo Pereira de Aloise, Antonio Carlos [UNIFESP] Ferreira, Lydia Masako [UNIFESP] |
author_role |
author |
author2 |
Gaiba, Silvana França, Jerônimo Pereira de Aloise, Antonio Carlos [UNIFESP] Ferreira, Lydia Masako [UNIFESP] |
author2_role |
author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade Estadual de Santa Cruz Department of Surgery |
dc.contributor.author.fl_str_mv |
Suartz, Caio Vinicius Gaiba, Silvana França, Jerônimo Pereira de Aloise, Antonio Carlos [UNIFESP] Ferreira, Lydia Masako [UNIFESP] |
dc.subject.eng.fl_str_mv |
Surgical Flaps Rats Adult Stem Cells Stem Cells Adipose Tissue |
topic |
Surgical Flaps Rats Adult Stem Cells Stem Cells Adipose Tissue |
description |
PURPOSE:To evaluate the viability of random pattern dorsal skin flaps in rats after injection of adipose-derived stem cells (ADSC).METHODS: Thirty five adult male Wistar EPM rats (weight 250-300 g) were distributed, at random, in two groups. I- Control (flap elevation with injection of saline solution) with fifteen animals and II- Experimental (flap elevation with injection of ADSC ) with fifteen animal. The ADSC were isolated from others five adult male rats. A dorsal skin flap measuring 10x4 cm was raised and a plastic barrier was placed between the flap and its bed in both groups and the injection (cells or saline solution) were perfomed immediately after the surgery. The percentage of flap necrosis was measured on the seventh postoperative day.RESULTS:The ADSC were able to replicate in our culture conditions. We also induced their adipogenic, osteogenic and chondrogenic differentiation to verify their mesenchymal stem cells potentiality in vitro. The results were statistically significant showing that the ADSC decreased the area of necrosis (p<0.05).CONCLUSIONS:The cells demonstrated adipogenic, osteogenic and chondrogenic differentiation potential in vitro. The administration of adipose-derived stem cells was effective to increase the viability of the random random pattern dorsal skin flaps in rats. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-01-01 |
dc.date.accessioned.fl_str_mv |
2015-06-14T13:46:54Z |
dc.date.available.fl_str_mv |
2015-06-14T13:46:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 02-05, 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/8208 http://dx.doi.org/10.1590/S0102-86502014001700001 |
dc.identifier.issn.none.fl_str_mv |
0102-8650 |
dc.identifier.file.none.fl_str_mv |
S0102-86502014001500002.pdf |
dc.identifier.scielo.none.fl_str_mv |
S0102-86502014001500002 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S0102-86502014001700001 |
dc.identifier.wos.none.fl_str_mv |
WOS:000344466300002 |
identifier_str_mv |
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 02-05, 2014. 0102-8650 S0102-86502014001500002.pdf S0102-86502014001500002 10.1590/S0102-86502014001700001 WOS:000344466300002 |
url |
http://repositorio.unifesp.br/handle/11600/8208 http://dx.doi.org/10.1590/S0102-86502014001700001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Acta Cirurgica Brasileira |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
02-05 |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
bitstream.url.fl_str_mv |
${dspace.ui.url}/bitstream/11600/8208/1/S0102-86502014001500002.pdf ${dspace.ui.url}/bitstream/11600/8208/2/S0102-86502014001500002.pdf.txt |
bitstream.checksum.fl_str_mv |
4145a0cd99176ee031fd99c5f1756adb 3ea2f8b60f12f4a1243bd204af632f83 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1783460292257120256 |