Hidroxiapatita nano e microparticulada: interação óssea
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/58420 |
Resumo: | Introduction: Tissue Engineering (ET) is the science that associates biological knowledge with Engineering fundamentals. ET is based on the use of cell-seeded biocompatible materials (scaffolds) that have the ability to differentiate into tissues. Among these biomaterial, hydroxyapatite (HA) is the most studied for ET of mineralized tissues. The evaluation of the biomaterial of HA is fundamental to collaborate with the bone regeneration. Aim: To evaluate the interaction of hydroxyapatite implants nano and microparticulate in rabbit tíbia. Methods: Fifty-six hydroxyapatite implants was implanted on twenty- eigth rabbit tíbia. 28 implants was nano particulate hydroxyapatite and 28 micro particulate hydroxyapatite, 14 nano and micro particulate implants was sintered by ultrasound (US) and 14 sintered by magnetic stirring (MS). Three holes were made in the tibia of the rabbits, in the proximal holes were implanted the nano particulate biomaterials (positive control), in the central holes were implanted the micro particulate (experimental sample) and, the distal orifice was empty (negative control). Three radiographic controls were performed with a 14-day interval. After 28 days the painless induced death was performed and the specimens were harvested and submitted to histological analysis and MicroCT. Results: The positive controls MS and US presented lower bone growth and greater porosity in relation to the experimental samples, whereas the negative controls had higher bone growth and lower porosity. Conclusions: The use of a nano particulate hydroxyapatite biomaterial promotes greater bone growth with lower porosity, collaborating with ET of mineralized tissues. |
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Hidroxiapatita nano e microparticulada: interação ósseaNano and microparticulate hydroxyapatite: bone interactionHydroxyapatiteTissue EngineeringBiomaterialsHidroxiapatitaEngenharia TecidualBiomateriaisIntroduction: Tissue Engineering (ET) is the science that associates biological knowledge with Engineering fundamentals. ET is based on the use of cell-seeded biocompatible materials (scaffolds) that have the ability to differentiate into tissues. Among these biomaterial, hydroxyapatite (HA) is the most studied for ET of mineralized tissues. The evaluation of the biomaterial of HA is fundamental to collaborate with the bone regeneration. Aim: To evaluate the interaction of hydroxyapatite implants nano and microparticulate in rabbit tíbia. Methods: Fifty-six hydroxyapatite implants was implanted on twenty- eigth rabbit tíbia. 28 implants was nano particulate hydroxyapatite and 28 micro particulate hydroxyapatite, 14 nano and micro particulate implants was sintered by ultrasound (US) and 14 sintered by magnetic stirring (MS). Three holes were made in the tibia of the rabbits, in the proximal holes were implanted the nano particulate biomaterials (positive control), in the central holes were implanted the micro particulate (experimental sample) and, the distal orifice was empty (negative control). Three radiographic controls were performed with a 14-day interval. After 28 days the painless induced death was performed and the specimens were harvested and submitted to histological analysis and MicroCT. Results: The positive controls MS and US presented lower bone growth and greater porosity in relation to the experimental samples, whereas the negative controls had higher bone growth and lower porosity. Conclusions: The use of a nano particulate hydroxyapatite biomaterial promotes greater bone growth with lower porosity, collaborating with ET of mineralized tissues.Introdução: A Engenharia Tecidual (ET) é a ciência que associa conhecimentos biológicos com fundamentos da Engenharia. A ET baseia-se na utilização de materiais biocompatíveis (scaffolds) semeados com células (construct) que possuem a capacidade de se diferenciar em tecidos. Entre esses biomateriais, a hidroxiapatita (HA) é o mais estudado para a ET dos tecidos mineralizados. A avaliação do biomaterial de HA é fundamental para colaborar com a regeneração óssea. Objetivo: Avaliar a interação dos implantes de HA nano e micro particulada em tíbia de coelhos. Método: Foram implantados cirurgicamente 56 implantes de HA em tíbias de 28 coelhos, 28 implantes eram de HA nano particulada e 28 de HA micro particulada, sendo 14 nano e micro particuladas sinterizados por ultrassom (US) e 14 sinterizados por agitação magnética (AM). Três orifícios foram realizados nas tíbias dos coelhos, nos orifícios proximais foram implantados os biomateriais nano particulados (controle positivo), nos orifícios centrais micro particulados (amostra experimental), e o orifício distal ficou vazio (controle negativo). Três controles radiográficos foram realizados com intervalo de 14 dias. Após 28 dias a morte indolor induzida foi realizada e os espécimes foram colhidos e submetidos à análise histológica e MicroCT. Resultados: Os controles positivos AM e US apresentaram menor crescimento ósseo e maior porosidade em relação às amostras experimentais, já os controles negativos maior crescimento ósseo e menor porosidade. Conclusões: A utilização de um biomaterial de HA nano particulada promove maior crescimento ósseo com menor porosidade, colaborando com a ET de tecidos mineralizados.Universidade Federal de São Paulo (UNIFESP)Duailibi, Silvio Eduardo [UNIFESP]Duailibi, Monica Talarico [UNIFESP]http://lattes.cnpq.br/9581388138152475http://lattes.cnpq.br/3165201570670403http://lattes.cnpq.br/6829631262890543Universidade Federal de São Paulo (UNIFESP)Segala, Renato Dalcin [UNIFESP]2020-10-06T12:51:54Z2020-10-06T12:51:54Z2019info:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/publishedVersion101 f.application/pdfSEGALA, Renato Dalcin. Hidroxiapatita nano e microparticulada: interação óssea. 2019.99f. Dissertação (Mestrado em Cirurgia Translacional) – Escola Paulista de Medicina, Universidade Federal de São Paulo. São Paulo, 2019.RENATO DALCIN SEGALA -A.pdfhttps://repositorio.unifesp.br/handle/11600/58420porSão Pauloinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-02T05:29:28Zoai:repositorio.unifesp.br/:11600/58420Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-02T05:29:28Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Hidroxiapatita nano e microparticulada: interação óssea Nano and microparticulate hydroxyapatite: bone interaction |
title |
Hidroxiapatita nano e microparticulada: interação óssea |
spellingShingle |
Hidroxiapatita nano e microparticulada: interação óssea Segala, Renato Dalcin [UNIFESP] Hydroxyapatite Tissue Engineering Biomaterials Hidroxiapatita Engenharia Tecidual Biomateriais |
title_short |
Hidroxiapatita nano e microparticulada: interação óssea |
title_full |
Hidroxiapatita nano e microparticulada: interação óssea |
title_fullStr |
Hidroxiapatita nano e microparticulada: interação óssea |
title_full_unstemmed |
Hidroxiapatita nano e microparticulada: interação óssea |
title_sort |
Hidroxiapatita nano e microparticulada: interação óssea |
author |
Segala, Renato Dalcin [UNIFESP] |
author_facet |
Segala, Renato Dalcin [UNIFESP] |
author_role |
author |
dc.contributor.none.fl_str_mv |
Duailibi, Silvio Eduardo [UNIFESP] Duailibi, Monica Talarico [UNIFESP] http://lattes.cnpq.br/9581388138152475 http://lattes.cnpq.br/3165201570670403 http://lattes.cnpq.br/6829631262890543 Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Segala, Renato Dalcin [UNIFESP] |
dc.subject.por.fl_str_mv |
Hydroxyapatite Tissue Engineering Biomaterials Hidroxiapatita Engenharia Tecidual Biomateriais |
topic |
Hydroxyapatite Tissue Engineering Biomaterials Hidroxiapatita Engenharia Tecidual Biomateriais |
description |
Introduction: Tissue Engineering (ET) is the science that associates biological knowledge with Engineering fundamentals. ET is based on the use of cell-seeded biocompatible materials (scaffolds) that have the ability to differentiate into tissues. Among these biomaterial, hydroxyapatite (HA) is the most studied for ET of mineralized tissues. The evaluation of the biomaterial of HA is fundamental to collaborate with the bone regeneration. Aim: To evaluate the interaction of hydroxyapatite implants nano and microparticulate in rabbit tíbia. Methods: Fifty-six hydroxyapatite implants was implanted on twenty- eigth rabbit tíbia. 28 implants was nano particulate hydroxyapatite and 28 micro particulate hydroxyapatite, 14 nano and micro particulate implants was sintered by ultrasound (US) and 14 sintered by magnetic stirring (MS). Three holes were made in the tibia of the rabbits, in the proximal holes were implanted the nano particulate biomaterials (positive control), in the central holes were implanted the micro particulate (experimental sample) and, the distal orifice was empty (negative control). Three radiographic controls were performed with a 14-day interval. After 28 days the painless induced death was performed and the specimens were harvested and submitted to histological analysis and MicroCT. Results: The positive controls MS and US presented lower bone growth and greater porosity in relation to the experimental samples, whereas the negative controls had higher bone growth and lower porosity. Conclusions: The use of a nano particulate hydroxyapatite biomaterial promotes greater bone growth with lower porosity, collaborating with ET of mineralized tissues. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 2020-10-06T12:51:54Z 2020-10-06T12:51:54Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SEGALA, Renato Dalcin. Hidroxiapatita nano e microparticulada: interação óssea. 2019.99f. Dissertação (Mestrado em Cirurgia Translacional) – Escola Paulista de Medicina, Universidade Federal de São Paulo. São Paulo, 2019. RENATO DALCIN SEGALA -A.pdf https://repositorio.unifesp.br/handle/11600/58420 |
identifier_str_mv |
SEGALA, Renato Dalcin. Hidroxiapatita nano e microparticulada: interação óssea. 2019.99f. Dissertação (Mestrado em Cirurgia Translacional) – Escola Paulista de Medicina, Universidade Federal de São Paulo. São Paulo, 2019. RENATO DALCIN SEGALA -A.pdf |
url |
https://repositorio.unifesp.br/handle/11600/58420 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
101 f. application/pdf |
dc.coverage.none.fl_str_mv |
São Paulo |
dc.publisher.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
publisher.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
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1814268328858877952 |