Hidroxiapatita nano e microparticulada: interação óssea

Detalhes bibliográficos
Autor(a) principal: Segala, Renato Dalcin [UNIFESP]
Data de Publicação: 2019
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: https://repositorio.unifesp.br/handle/11600/58420
Resumo: Introduction: Tissue Engineering (ET) is the science that associates biological knowledge with Engineering fundamentals. ET is based on the use of cell-seeded biocompatible materials (scaffolds) that have the ability to differentiate into tissues. Among these biomaterial, hydroxyapatite (HA) is the most studied for ET of mineralized tissues. The evaluation of the biomaterial of HA is fundamental to collaborate with the bone regeneration. Aim: To evaluate the interaction of hydroxyapatite implants nano and microparticulate in rabbit tíbia. Methods: Fifty-six hydroxyapatite implants was implanted on twenty- eigth rabbit tíbia. 28 implants was nano particulate hydroxyapatite and 28 micro particulate hydroxyapatite, 14 nano and micro particulate implants was sintered by ultrasound (US) and 14 sintered by magnetic stirring (MS). Three holes were made in the tibia of the rabbits, in the proximal holes were implanted the nano particulate biomaterials (positive control), in the central holes were implanted the micro particulate (experimental sample) and, the distal orifice was empty (negative control). Three radiographic controls were performed with a 14-day interval. After 28 days the painless induced death was performed and the specimens were harvested and submitted to histological analysis and MicroCT. Results: The positive controls MS and US presented lower bone growth and greater porosity in relation to the experimental samples, whereas the negative controls had higher bone growth and lower porosity. Conclusions: The use of a nano particulate hydroxyapatite biomaterial promotes greater bone growth with lower porosity, collaborating with ET of mineralized tissues.
id UFSP_ce4dd54a87fa3781aa5fc8d951cc0892
oai_identifier_str oai:repositorio.unifesp.br/:11600/58420
network_acronym_str UFSP
network_name_str Repositório Institucional da UNIFESP
repository_id_str 3465
spelling Hidroxiapatita nano e microparticulada: interação ósseaNano and microparticulate hydroxyapatite: bone interactionHydroxyapatiteTissue EngineeringBiomaterialsHidroxiapatitaEngenharia TecidualBiomateriaisIntroduction: Tissue Engineering (ET) is the science that associates biological knowledge with Engineering fundamentals. ET is based on the use of cell-seeded biocompatible materials (scaffolds) that have the ability to differentiate into tissues. Among these biomaterial, hydroxyapatite (HA) is the most studied for ET of mineralized tissues. The evaluation of the biomaterial of HA is fundamental to collaborate with the bone regeneration. Aim: To evaluate the interaction of hydroxyapatite implants nano and microparticulate in rabbit tíbia. Methods: Fifty-six hydroxyapatite implants was implanted on twenty- eigth rabbit tíbia. 28 implants was nano particulate hydroxyapatite and 28 micro particulate hydroxyapatite, 14 nano and micro particulate implants was sintered by ultrasound (US) and 14 sintered by magnetic stirring (MS). Three holes were made in the tibia of the rabbits, in the proximal holes were implanted the nano particulate biomaterials (positive control), in the central holes were implanted the micro particulate (experimental sample) and, the distal orifice was empty (negative control). Three radiographic controls were performed with a 14-day interval. After 28 days the painless induced death was performed and the specimens were harvested and submitted to histological analysis and MicroCT. Results: The positive controls MS and US presented lower bone growth and greater porosity in relation to the experimental samples, whereas the negative controls had higher bone growth and lower porosity. Conclusions: The use of a nano particulate hydroxyapatite biomaterial promotes greater bone growth with lower porosity, collaborating with ET of mineralized tissues.Introdução: A Engenharia Tecidual (ET) é a ciência que associa conhecimentos biológicos com fundamentos da Engenharia. A ET baseia-se na utilização de materiais biocompatíveis (scaffolds) semeados com células (construct) que possuem a capacidade de se diferenciar em tecidos. Entre esses biomateriais, a hidroxiapatita (HA) é o mais estudado para a ET dos tecidos mineralizados. A avaliação do biomaterial de HA é fundamental para colaborar com a regeneração óssea. Objetivo: Avaliar a interação dos implantes de HA nano e micro particulada em tíbia de coelhos. Método: Foram implantados cirurgicamente 56 implantes de HA em tíbias de 28 coelhos, 28 implantes eram de HA nano particulada e 28 de HA micro particulada, sendo 14 nano e micro particuladas sinterizados por ultrassom (US) e 14 sinterizados por agitação magnética (AM). Três orifícios foram realizados nas tíbias dos coelhos, nos orifícios proximais foram implantados os biomateriais nano particulados (controle positivo), nos orifícios centrais micro particulados (amostra experimental), e o orifício distal ficou vazio (controle negativo). Três controles radiográficos foram realizados com intervalo de 14 dias. Após 28 dias a morte indolor induzida foi realizada e os espécimes foram colhidos e submetidos à análise histológica e MicroCT. Resultados: Os controles positivos AM e US apresentaram menor crescimento ósseo e maior porosidade em relação às amostras experimentais, já os controles negativos maior crescimento ósseo e menor porosidade. Conclusões: A utilização de um biomaterial de HA nano particulada promove maior crescimento ósseo com menor porosidade, colaborando com a ET de tecidos mineralizados.Universidade Federal de São Paulo (UNIFESP)Duailibi, Silvio Eduardo [UNIFESP]Duailibi, Monica Talarico [UNIFESP]http://lattes.cnpq.br/9581388138152475http://lattes.cnpq.br/3165201570670403http://lattes.cnpq.br/6829631262890543Universidade Federal de São Paulo (UNIFESP)Segala, Renato Dalcin [UNIFESP]2020-10-06T12:51:54Z2020-10-06T12:51:54Z2019info:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/publishedVersion101 f.application/pdfSEGALA, Renato Dalcin. Hidroxiapatita nano e microparticulada: interação óssea. 2019.99f. Dissertação (Mestrado em Cirurgia Translacional) – Escola Paulista de Medicina, Universidade Federal de São Paulo. São Paulo, 2019.RENATO DALCIN SEGALA -A.pdfhttps://repositorio.unifesp.br/handle/11600/58420porSão Pauloinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-02T05:29:28Zoai:repositorio.unifesp.br/:11600/58420Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-02T05:29:28Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Hidroxiapatita nano e microparticulada: interação óssea
Nano and microparticulate hydroxyapatite: bone interaction
title Hidroxiapatita nano e microparticulada: interação óssea
spellingShingle Hidroxiapatita nano e microparticulada: interação óssea
Segala, Renato Dalcin [UNIFESP]
Hydroxyapatite
Tissue Engineering
Biomaterials
Hidroxiapatita
Engenharia Tecidual
Biomateriais
title_short Hidroxiapatita nano e microparticulada: interação óssea
title_full Hidroxiapatita nano e microparticulada: interação óssea
title_fullStr Hidroxiapatita nano e microparticulada: interação óssea
title_full_unstemmed Hidroxiapatita nano e microparticulada: interação óssea
title_sort Hidroxiapatita nano e microparticulada: interação óssea
author Segala, Renato Dalcin [UNIFESP]
author_facet Segala, Renato Dalcin [UNIFESP]
author_role author
dc.contributor.none.fl_str_mv Duailibi, Silvio Eduardo [UNIFESP]
Duailibi, Monica Talarico [UNIFESP]
http://lattes.cnpq.br/9581388138152475
http://lattes.cnpq.br/3165201570670403
http://lattes.cnpq.br/6829631262890543
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Segala, Renato Dalcin [UNIFESP]
dc.subject.por.fl_str_mv Hydroxyapatite
Tissue Engineering
Biomaterials
Hidroxiapatita
Engenharia Tecidual
Biomateriais
topic Hydroxyapatite
Tissue Engineering
Biomaterials
Hidroxiapatita
Engenharia Tecidual
Biomateriais
description Introduction: Tissue Engineering (ET) is the science that associates biological knowledge with Engineering fundamentals. ET is based on the use of cell-seeded biocompatible materials (scaffolds) that have the ability to differentiate into tissues. Among these biomaterial, hydroxyapatite (HA) is the most studied for ET of mineralized tissues. The evaluation of the biomaterial of HA is fundamental to collaborate with the bone regeneration. Aim: To evaluate the interaction of hydroxyapatite implants nano and microparticulate in rabbit tíbia. Methods: Fifty-six hydroxyapatite implants was implanted on twenty- eigth rabbit tíbia. 28 implants was nano particulate hydroxyapatite and 28 micro particulate hydroxyapatite, 14 nano and micro particulate implants was sintered by ultrasound (US) and 14 sintered by magnetic stirring (MS). Three holes were made in the tibia of the rabbits, in the proximal holes were implanted the nano particulate biomaterials (positive control), in the central holes were implanted the micro particulate (experimental sample) and, the distal orifice was empty (negative control). Three radiographic controls were performed with a 14-day interval. After 28 days the painless induced death was performed and the specimens were harvested and submitted to histological analysis and MicroCT. Results: The positive controls MS and US presented lower bone growth and greater porosity in relation to the experimental samples, whereas the negative controls had higher bone growth and lower porosity. Conclusions: The use of a nano particulate hydroxyapatite biomaterial promotes greater bone growth with lower porosity, collaborating with ET of mineralized tissues.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020-10-06T12:51:54Z
2020-10-06T12:51:54Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv SEGALA, Renato Dalcin. Hidroxiapatita nano e microparticulada: interação óssea. 2019.99f. Dissertação (Mestrado em Cirurgia Translacional) – Escola Paulista de Medicina, Universidade Federal de São Paulo. São Paulo, 2019.
RENATO DALCIN SEGALA -A.pdf
https://repositorio.unifesp.br/handle/11600/58420
identifier_str_mv SEGALA, Renato Dalcin. Hidroxiapatita nano e microparticulada: interação óssea. 2019.99f. Dissertação (Mestrado em Cirurgia Translacional) – Escola Paulista de Medicina, Universidade Federal de São Paulo. São Paulo, 2019.
RENATO DALCIN SEGALA -A.pdf
url https://repositorio.unifesp.br/handle/11600/58420
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 101 f.
application/pdf
dc.coverage.none.fl_str_mv São Paulo
dc.publisher.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
publisher.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268328858877952