PHARMACOLOGICAL CHARACTERIZATION OF RABBIT CORPUS CAVERNOSUM RELAXATION MEDIATED BY THE TISSUE KALLIKREIN-KININ SYSTEM

• Autor(a) principal: Lopes-Martins, Rodrigo A. B
• Data de Publicação: 1994
• Outros Autores: Antunes, Edson, Oliva, Maria Luiza Vilela [UNIFESP], Sampaio, Claudio Augusto Machado [UNIFESP], Burton, J, Denucci, G
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• dARK ID: ark:/48912/001300000mf3v
• Texto Completo: http://onlinelibrary.wiley.com/doi/10.1111/j.1476-5381.1994.tb16177.x/full; http://repositorio.unifesp.br/handle/11600/43177
• topic: KININ RECEPTORS; PENILE ERECTION; PRIAPISM; HOE 140; APROTININ; PROKALLIKREINS; KIZD-06; PHONEUTRIA NIGRIVENTER VENOM

1 The roles of the tissue kallikrein-kinin system and nitric oxide (NO) release in Phoneutria nigriventer venom-induced relaxations of rabbit corpus cavernosum (RbCC) smooth muscle have been investigated by use of a bioassay cascade.2 Phoneutria nigriventer venom (10-30 mu g), porcine pancreatic kallikrein (100 mu), rabbit urinary kallikrein (10 mu), bradykinin (BK, 0.3-3 nmol), acetylcholine (ACh, 0.3-30 nmol) and glyceryl trinitrate (GTN, 0.5-10 nmol) caused relaxations of the RbCC strips. Captopril (1 mu M) substantially potentiated Phoneutria nigriventer venom- and BK-induced RbCC relaxations without affecting those elicited by GTN.3 The bradykinin B-2 receptor antagonist, Hoe 140 (D-Arg-[Hyp(3),Thi(5), D-Tic(7),Oic(8)]-BK, 50 nM), aprotinin (10 mu g ml(-1)) and the tissue kallikrein inhibitor, Pro-Phe-Aph-Ser-Val-Gln-NH2 (KIZD-06, 1.3 mu M) significantly inhibited Phoneutria nigriventer venom-induced RbCC relaxations, without affecting those provoked by GTN and ACh. The B-1 receptor antagonist, [Leu(9)]des Arg(10)BK (0.5 mu M) and soybean trypsin inhibitor (SBTI, 10 mu g ml(-1)) had no effect on Phoneutria nigriventer venom-induced RbCC relaxations.4 The relaxations induced by Phoneutria nigriventer venom, porcine pancreas kallikrein, BK and ACh were significantly inhibited by N-omega-nitro-L-arginine methyl ester (L-NAME, 10 mu M) but not by D-NAME (10 mu M). L-NAME did not affect GTN-induced relaxations. L-Arginine (300 mu M), but not D-arginine (300 mu M), significantly reversed the inhibitory effect of L-NAME.5 Our results indicate that Phoneutria nigriventer venom activates the tissue kallikrein-kininogen-kinin system in RbCC strips leading to NO release and suggest a functional role for this system in penile erection.