High-resolution proteomic profiling of spider venom: expanding the toxin diversity of Phoneutria nigriventer venom

Detalhes bibliográficos
Autor(a) principal: Liberato, Tarcisio [UNIFESP]
Data de Publicação: 2016
Outros Autores: Paolo Troncone, Lanfranco Ranieri, Yamashiro, Edson T., Serrano, Solange M. T., Zelanis, Andre [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
dARK ID: ark:/48912/001300000bvz0
Texto Completo: http://dx.doi.org/10.1007/s00726-015-2151-6
https://repositorio.unifesp.br/handle/11600/57917
Resumo: Here we present a proteomic characterization of Phoneutria nigriventer venom. A shotgun proteomic approach allowed the identification, for the first time, of O-glycosyl hydrolases (chitinases) in P. nigriventer venom. The electrophoretic profiles under nonreducing and reducing conditions, and protein identification by mass spectrometry, indicated the presence of oligomeric toxin structures in the venom. Complementary proteomic approaches allowed for a qualitative and semi-quantitative profiling of P. nigriventer venom complexity, expanding its known venom proteome diversity.
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spelling High-resolution proteomic profiling of spider venom: expanding the toxin diversity of Phoneutria nigriventer venomPhoneutria nigriventerProteomeSpider venomChitinaseHere we present a proteomic characterization of Phoneutria nigriventer venom. A shotgun proteomic approach allowed the identification, for the first time, of O-glycosyl hydrolases (chitinases) in P. nigriventer venom. The electrophoretic profiles under nonreducing and reducing conditions, and protein identification by mass spectrometry, indicated the presence of oligomeric toxin structures in the venom. Complementary proteomic approaches allowed for a qualitative and semi-quantitative profiling of P. nigriventer venom complexity, expanding its known venom proteome diversity.Inst Butantan, Farmacol Lab, Sao Paulo, BrazilUniv Fed Sao Paulo, Lab Prote Func, Dept Ciencia & Tecnol, ICT UNIFESP, Campus Sao Jose dos Campos,Rua Talim 330, BR-12231280 Sao Paulo, SP, BrazilInst Butantan, Lab Especial Toxinol Aplicada, Ctr Toxins Immune Response & Cell Signaling CeTIC, Sao Paulo, BrazilUniv Fed Sao Paulo, Lab Prote Func, Dept Ciencia & Tecnol, ICT UNIFESP, Campus Sao Jose dos Campos,Rua Talim 330, BR-12231280 Sao Paulo, SP, BrazilWeb of ScienceFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)FAPESP: 11/08514-8FAPESP: 13/07467-1FAPESP: 02/04545-7Springer Wien2020-08-21T17:00:14Z2020-08-21T17:00:14Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion901-906http://dx.doi.org/10.1007/s00726-015-2151-6Amino Acids. Wien, v. 48, n. 3, p. 901-906, 2016.10.1007/s00726-015-2151-60939-4451https://repositorio.unifesp.br/handle/11600/57917WOS:000370817900022ark:/48912/001300000bvz0engAmino AcidsWieninfo:eu-repo/semantics/openAccessLiberato, Tarcisio [UNIFESP]Paolo Troncone, Lanfranco RanieriYamashiro, Edson T.Serrano, Solange M. T.Zelanis, Andre [UNIFESP]reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2021-09-29T09:32:03Zoai:repositorio.unifesp.br/:11600/57917Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T20:09:58.031428Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv High-resolution proteomic profiling of spider venom: expanding the toxin diversity of Phoneutria nigriventer venom
title High-resolution proteomic profiling of spider venom: expanding the toxin diversity of Phoneutria nigriventer venom
spellingShingle High-resolution proteomic profiling of spider venom: expanding the toxin diversity of Phoneutria nigriventer venom
Liberato, Tarcisio [UNIFESP]
Phoneutria nigriventer
Proteome
Spider venom
Chitinase
title_short High-resolution proteomic profiling of spider venom: expanding the toxin diversity of Phoneutria nigriventer venom
title_full High-resolution proteomic profiling of spider venom: expanding the toxin diversity of Phoneutria nigriventer venom
title_fullStr High-resolution proteomic profiling of spider venom: expanding the toxin diversity of Phoneutria nigriventer venom
title_full_unstemmed High-resolution proteomic profiling of spider venom: expanding the toxin diversity of Phoneutria nigriventer venom
title_sort High-resolution proteomic profiling of spider venom: expanding the toxin diversity of Phoneutria nigriventer venom
author Liberato, Tarcisio [UNIFESP]
author_facet Liberato, Tarcisio [UNIFESP]
Paolo Troncone, Lanfranco Ranieri
Yamashiro, Edson T.
Serrano, Solange M. T.
Zelanis, Andre [UNIFESP]
author_role author
author2 Paolo Troncone, Lanfranco Ranieri
Yamashiro, Edson T.
Serrano, Solange M. T.
Zelanis, Andre [UNIFESP]
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Liberato, Tarcisio [UNIFESP]
Paolo Troncone, Lanfranco Ranieri
Yamashiro, Edson T.
Serrano, Solange M. T.
Zelanis, Andre [UNIFESP]
dc.subject.por.fl_str_mv Phoneutria nigriventer
Proteome
Spider venom
Chitinase
topic Phoneutria nigriventer
Proteome
Spider venom
Chitinase
description Here we present a proteomic characterization of Phoneutria nigriventer venom. A shotgun proteomic approach allowed the identification, for the first time, of O-glycosyl hydrolases (chitinases) in P. nigriventer venom. The electrophoretic profiles under nonreducing and reducing conditions, and protein identification by mass spectrometry, indicated the presence of oligomeric toxin structures in the venom. Complementary proteomic approaches allowed for a qualitative and semi-quantitative profiling of P. nigriventer venom complexity, expanding its known venom proteome diversity.
publishDate 2016
dc.date.none.fl_str_mv 2016
2020-08-21T17:00:14Z
2020-08-21T17:00:14Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00726-015-2151-6
Amino Acids. Wien, v. 48, n. 3, p. 901-906, 2016.
10.1007/s00726-015-2151-6
0939-4451
https://repositorio.unifesp.br/handle/11600/57917
WOS:000370817900022
dc.identifier.dark.fl_str_mv ark:/48912/001300000bvz0
url http://dx.doi.org/10.1007/s00726-015-2151-6
https://repositorio.unifesp.br/handle/11600/57917
identifier_str_mv Amino Acids. Wien, v. 48, n. 3, p. 901-906, 2016.
10.1007/s00726-015-2151-6
0939-4451
WOS:000370817900022
ark:/48912/001300000bvz0
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Amino Acids
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 901-906
dc.coverage.none.fl_str_mv Wien
dc.publisher.none.fl_str_mv Springer Wien
publisher.none.fl_str_mv Springer Wien
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1818602440203698176

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