Drifter technique: a new method to obtain metaphases in Hep-2 cell line cultures

• Autor(a) principal: Lima, Eleonidas Moura
• Data de Publicação: 2005
• Outros Autores: Rissino, Jorge Dores, Guimarães, Adriana Costa, Overal, David James, Khayat, André Salim, Souza, Patrícia Carvalho de, Christofolini, Denise Maria [UNIFESP], Smith, Marilia de Arruda Cardoso [UNIFESP], Burbano, Rommel Rodríguez [UNIFESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.1590/S1516-89132005000500005; http://repositorio.unifesp.br/handle/11600/2590
• topic: Hep-2 cell line; drifter technique; mitotic index

The Hep-2 cell line is derived from laryngeal carcinoma cells and is often utilized as a model in carcinogenesis and mutagenesis tests. To evaluate the proliferative potential of this line, we developed a cytogenetic methodology (drifter technique) to obtain metaphases from cells that loose cellular adhesion when they underwent mitosis in culture. By this procedure, 2000 cells were counted, resulting in a mitotic index (MI) of 22.2%. Although this MI was not statistically different from the one obtained using either a classical cytogenetic method or a cell synchronization technique, the drifter technique has the advantage of not requiring the use of some reagents for the obtention of metaphases and also of diminishing the consumption of maintenance reagents for this cell line.