A TSH-CREB1-microRNA Loop Is Required for Thyroid Cell Growth
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/34079 http://dx.doi.org/10.1210/me.2011-0014 |
Resumo: | MicroRNA (miRNA or miR) are an important class of regulators that participate in such biological functions as development, cell proliferation, differentiation, and apoptosis. the aim of this study was to elucidate the role of miRNA in cell proliferation using a unique cell system, namely thyroid cells that require thyrotropin for their growth. Here, we report the identification of a set of five specific miRNA (miR-1, miR-28-A, miR-290-5p, miR-296-3p, and miR-297a), whose down-regulation by thyrotropin is required for thyroid cell growth. in fact, overexpression of these miRNA negatively affects cell growth. We show that three of these miRNA target cAMP-responsive element binding protein (CREB)1, a thyrotropin-activated transcription factor, and that CREB1 binds the regulatory regions of the down-regulated miRNA. Hence, these data indicate that a synergistic loop involving thyrotropin, CREB1, and miRNA is required for thyroid cell proliferation. (Molecular Endocrinology 25: 1819-1830, 2011) |
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Leone, VincenzaD'Angelo, DanielaFerraro, AngeloPallante, PierlorenzoRubio, Ileana [UNIFESP]Santoro, MassimoCroce, Carlo MariaFusco, AlfredoUniv Naples Federico 2CEINGE Ctr Ingn Genet Biotecnol AvanzateEuropean Sch Mol MedUniversidade Federal de São Paulo (UNIFESP)Ohio State Univ2016-01-24T14:17:15Z2016-01-24T14:17:15Z2011-10-01Molecular Endocrinology. Chevy Chase: Endocrine Soc, v. 25, n. 10, p. 1819-1830, 2011.0888-8809http://repositorio.unifesp.br/handle/11600/34079http://dx.doi.org/10.1210/me.2011-001410.1210/me.2011-0014WOS:000295322700012MicroRNA (miRNA or miR) are an important class of regulators that participate in such biological functions as development, cell proliferation, differentiation, and apoptosis. the aim of this study was to elucidate the role of miRNA in cell proliferation using a unique cell system, namely thyroid cells that require thyrotropin for their growth. Here, we report the identification of a set of five specific miRNA (miR-1, miR-28-A, miR-290-5p, miR-296-3p, and miR-297a), whose down-regulation by thyrotropin is required for thyroid cell growth. in fact, overexpression of these miRNA negatively affects cell growth. We show that three of these miRNA target cAMP-responsive element binding protein (CREB)1, a thyrotropin-activated transcription factor, and that CREB1 binds the regulatory regions of the down-regulated miRNA. Hence, these data indicate that a synergistic loop involving thyrotropin, CREB1, and miRNA is required for thyroid cell proliferation. (Molecular Endocrinology 25: 1819-1830, 2011)Associazione Italiana Ricerca sul CancroUniv Naples Federico 2, Ist Endocrinol & Oncol Sperimentale, CNR, Dipartimento Biol & Patol Cellulare & Mol,Fac Med, I-80131 Naples, ItalyCEINGE Ctr Ingn Genet Biotecnol Avanzate, Naples Oncogenom Ctr, Naples, ItalyEuropean Sch Mol Med, I-80145 Naples, ItalyUniversidade Federal de São Paulo, Dept Biol Sci, BR-05403 São Paulo, BrazilOhio State Univ, Ctr Comprehens Canc, Dept Mol Virol, Columbus, OH 43210 USAUniversidade Federal de São Paulo, Dept Biol Sci, BR-05403 São Paulo, BrazilWeb of Science1819-1830engEndocrine SocMolecular EndocrinologyA TSH-CREB1-microRNA Loop Is Required for Thyroid Cell Growthinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/340792022-07-08 10:54:11.648metadata only accessoai:repositorio.unifesp.br:11600/34079Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-07-08T13:54:11Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
A TSH-CREB1-microRNA Loop Is Required for Thyroid Cell Growth |
title |
A TSH-CREB1-microRNA Loop Is Required for Thyroid Cell Growth |
spellingShingle |
A TSH-CREB1-microRNA Loop Is Required for Thyroid Cell Growth Leone, Vincenza |
title_short |
A TSH-CREB1-microRNA Loop Is Required for Thyroid Cell Growth |
title_full |
A TSH-CREB1-microRNA Loop Is Required for Thyroid Cell Growth |
title_fullStr |
A TSH-CREB1-microRNA Loop Is Required for Thyroid Cell Growth |
title_full_unstemmed |
A TSH-CREB1-microRNA Loop Is Required for Thyroid Cell Growth |
title_sort |
A TSH-CREB1-microRNA Loop Is Required for Thyroid Cell Growth |
author |
Leone, Vincenza |
author_facet |
Leone, Vincenza D'Angelo, Daniela Ferraro, Angelo Pallante, Pierlorenzo Rubio, Ileana [UNIFESP] Santoro, Massimo Croce, Carlo Maria Fusco, Alfredo |
author_role |
author |
author2 |
D'Angelo, Daniela Ferraro, Angelo Pallante, Pierlorenzo Rubio, Ileana [UNIFESP] Santoro, Massimo Croce, Carlo Maria Fusco, Alfredo |
author2_role |
author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Univ Naples Federico 2 CEINGE Ctr Ingn Genet Biotecnol Avanzate European Sch Mol Med Universidade Federal de São Paulo (UNIFESP) Ohio State Univ |
dc.contributor.author.fl_str_mv |
Leone, Vincenza D'Angelo, Daniela Ferraro, Angelo Pallante, Pierlorenzo Rubio, Ileana [UNIFESP] Santoro, Massimo Croce, Carlo Maria Fusco, Alfredo |
description |
MicroRNA (miRNA or miR) are an important class of regulators that participate in such biological functions as development, cell proliferation, differentiation, and apoptosis. the aim of this study was to elucidate the role of miRNA in cell proliferation using a unique cell system, namely thyroid cells that require thyrotropin for their growth. Here, we report the identification of a set of five specific miRNA (miR-1, miR-28-A, miR-290-5p, miR-296-3p, and miR-297a), whose down-regulation by thyrotropin is required for thyroid cell growth. in fact, overexpression of these miRNA negatively affects cell growth. We show that three of these miRNA target cAMP-responsive element binding protein (CREB)1, a thyrotropin-activated transcription factor, and that CREB1 binds the regulatory regions of the down-regulated miRNA. Hence, these data indicate that a synergistic loop involving thyrotropin, CREB1, and miRNA is required for thyroid cell proliferation. (Molecular Endocrinology 25: 1819-1830, 2011) |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-10-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:17:15Z |
dc.date.available.fl_str_mv |
2016-01-24T14:17:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Molecular Endocrinology. Chevy Chase: Endocrine Soc, v. 25, n. 10, p. 1819-1830, 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/34079 http://dx.doi.org/10.1210/me.2011-0014 |
dc.identifier.issn.none.fl_str_mv |
0888-8809 |
dc.identifier.doi.none.fl_str_mv |
10.1210/me.2011-0014 |
dc.identifier.wos.none.fl_str_mv |
WOS:000295322700012 |
identifier_str_mv |
Molecular Endocrinology. Chevy Chase: Endocrine Soc, v. 25, n. 10, p. 1819-1830, 2011. 0888-8809 10.1210/me.2011-0014 WOS:000295322700012 |
url |
http://repositorio.unifesp.br/handle/11600/34079 http://dx.doi.org/10.1210/me.2011-0014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Molecular Endocrinology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1819-1830 |
dc.publisher.none.fl_str_mv |
Endocrine Soc |
publisher.none.fl_str_mv |
Endocrine Soc |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764155280687104 |