Immune response to tetanus booster in infants aged 15 months born prematurely with very low birth weight

Detalhes bibliográficos
Autor(a) principal: Perin, Maria Cristina Abrão Aued [UNIFESP]
Data de Publicação: 2012
Outros Autores: Schlindwein, Carolina Frank [UNIFESP], Moraes-Pinto, Maria Isabel de [UNIFESP], Simao-Gurge, Raquel Maria [UNIFESP], Mimica, Ana Flavia de Mello Almada [UNIFESP], Goulart, Ana Lucia [UNIFESP], Santos, Amélia Miyashiro Nunes dos [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://repositorio.unifesp.br/handle/11600/35412
http://dx.doi.org/10.1016/j.vaccine.2012.08.056
Resumo: Objectives: To compare humoral and cellular immune responses to tetanus booster vaccination in infants born prematurely with those born at full term and identify factors associated with the humoral response.Methods: A prospective study was carried out on children born prematurely and with a birth weight <1500g and with infants born at full term. At 15 months (pre-vaccination) and 18 months (post-vaccination), anti-tetanus antibodies were measured by ELISA; the intracellular interferon-gamma percentages of CD4+ T and CD8+ T cells after in vitro stimulation with tetanus toxoid were determined by flow cytometry. Chi-squared or Fisher's exact test was used to compare categorical variables. Student's t-test or Mann-Whitney test was used to compare numerical variables. Regression analysis was performed to determine factors associated with humoral immunity. Statistical significance was considered if p < 0.05.Results: Sixty-four premature and 54 full-term infants were studied. the proportion of children immune against tetanus at 15 and 18 months was similar in both groups. the geometric mean of the antibodies was lower among the premature children at 15 months (p = 0.025) and was similar in both groups at 18 months (p = 0.852). the percentages of CD4+ and CD8+ T cells expressing intracellular IFN-gamma were similar in both groups at 15 and 18 months. Gestational age <32 weeks was associated with a reduction of 0.116 IU/mL in the level of antibodies at 15 months. Breastfeeding >6 months was associated with a 3.5-fold greater chance of optimal protective (>= 0.1 IU/mL) antibody level against tetanus at 15 months and an increase of 0.956 in the level of antibodies at 18 months.Conclusions: Humoral and cellular response following a tetanus booster was similar in both groups. Premature infants exhibited lower levels of anti-tetanus antibodies at 15 months of age, with the lowest levels in those born at a gestational age of less than 32 weeks. Breastfeeding was associated with greater levels of antibody against tetanus. (C) 2012 Elsevier B.V. All rights reserved.
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spelling Perin, Maria Cristina Abrão Aued [UNIFESP]Schlindwein, Carolina Frank [UNIFESP]Moraes-Pinto, Maria Isabel de [UNIFESP]Simao-Gurge, Raquel Maria [UNIFESP]Mimica, Ana Flavia de Mello Almada [UNIFESP]Goulart, Ana Lucia [UNIFESP]Santos, Amélia Miyashiro Nunes dos [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2016-01-24T14:27:53Z2016-01-24T14:27:53Z2012-10-12Vaccine. Oxford: Elsevier B.V., v. 30, n. 46, p. 6521-6526, 2012.0264-410Xhttp://repositorio.unifesp.br/handle/11600/35412http://dx.doi.org/10.1016/j.vaccine.2012.08.056WOS000310419700003.pdf10.1016/j.vaccine.2012.08.056WOS:000310419700003Objectives: To compare humoral and cellular immune responses to tetanus booster vaccination in infants born prematurely with those born at full term and identify factors associated with the humoral response.Methods: A prospective study was carried out on children born prematurely and with a birth weight <1500g and with infants born at full term. At 15 months (pre-vaccination) and 18 months (post-vaccination), anti-tetanus antibodies were measured by ELISA; the intracellular interferon-gamma percentages of CD4+ T and CD8+ T cells after in vitro stimulation with tetanus toxoid were determined by flow cytometry. Chi-squared or Fisher's exact test was used to compare categorical variables. Student's t-test or Mann-Whitney test was used to compare numerical variables. Regression analysis was performed to determine factors associated with humoral immunity. Statistical significance was considered if p < 0.05.Results: Sixty-four premature and 54 full-term infants were studied. the proportion of children immune against tetanus at 15 and 18 months was similar in both groups. the geometric mean of the antibodies was lower among the premature children at 15 months (p = 0.025) and was similar in both groups at 18 months (p = 0.852). the percentages of CD4+ and CD8+ T cells expressing intracellular IFN-gamma were similar in both groups at 15 and 18 months. Gestational age <32 weeks was associated with a reduction of 0.116 IU/mL in the level of antibodies at 15 months. Breastfeeding >6 months was associated with a 3.5-fold greater chance of optimal protective (>= 0.1 IU/mL) antibody level against tetanus at 15 months and an increase of 0.956 in the level of antibodies at 18 months.Conclusions: Humoral and cellular response following a tetanus booster was similar in both groups. Premature infants exhibited lower levels of anti-tetanus antibodies at 15 months of age, with the lowest levels in those born at a gestational age of less than 32 weeks. Breastfeeding was associated with greater levels of antibody against tetanus. (C) 2012 Elsevier B.V. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Federal de São Paulo, Neonatal Div Med, Dept Pediat, São Paulo, BrazilUniversidade Federal de São Paulo, Div Pediat Infect Dis, Dept Pediat, São Paulo, BrazilUniversidade Federal de São Paulo, Neonatal Div Med, Dept Pediat, São Paulo, BrazilUniversidade Federal de São Paulo, Div Pediat Infect Dis, Dept Pediat, São Paulo, BrazilFAPESP: 06/51865-8FAPESP: 09/14351-4Web of Science6521-6526engElsevier B.V.Vaccinehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyinfo:eu-repo/semantics/openAccessPremature infantImmunizationTetanus toxoidHumoral immunityCellular immunityImmune response to tetanus booster in infants aged 15 months born prematurely with very low birth weightinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000310419700003.pdfapplication/pdf262359${dspace.ui.url}/bitstream/11600/35412/1/WOS000310419700003.pdfe37a84b04d7cef10d0330a113b7d9d08MD51open accessTEXTWOS000310419700003.pdf.txtWOS000310419700003.pdf.txtExtracted texttext/plain37277${dspace.ui.url}/bitstream/11600/35412/2/WOS000310419700003.pdf.txte6fb1e53b14aa6d58ef8b6a705554e1cMD52open access11600/354122022-06-02 09:34:58.742open accessoai:repositorio.unifesp.br:11600/35412Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:16:16.947130Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.en.fl_str_mv Immune response to tetanus booster in infants aged 15 months born prematurely with very low birth weight
title Immune response to tetanus booster in infants aged 15 months born prematurely with very low birth weight
spellingShingle Immune response to tetanus booster in infants aged 15 months born prematurely with very low birth weight
Perin, Maria Cristina Abrão Aued [UNIFESP]
Premature infant
Immunization
Tetanus toxoid
Humoral immunity
Cellular immunity
title_short Immune response to tetanus booster in infants aged 15 months born prematurely with very low birth weight
title_full Immune response to tetanus booster in infants aged 15 months born prematurely with very low birth weight
title_fullStr Immune response to tetanus booster in infants aged 15 months born prematurely with very low birth weight
title_full_unstemmed Immune response to tetanus booster in infants aged 15 months born prematurely with very low birth weight
title_sort Immune response to tetanus booster in infants aged 15 months born prematurely with very low birth weight
author Perin, Maria Cristina Abrão Aued [UNIFESP]
author_facet Perin, Maria Cristina Abrão Aued [UNIFESP]
Schlindwein, Carolina Frank [UNIFESP]
Moraes-Pinto, Maria Isabel de [UNIFESP]
Simao-Gurge, Raquel Maria [UNIFESP]
Mimica, Ana Flavia de Mello Almada [UNIFESP]
Goulart, Ana Lucia [UNIFESP]
Santos, Amélia Miyashiro Nunes dos [UNIFESP]
author_role author
author2 Schlindwein, Carolina Frank [UNIFESP]
Moraes-Pinto, Maria Isabel de [UNIFESP]
Simao-Gurge, Raquel Maria [UNIFESP]
Mimica, Ana Flavia de Mello Almada [UNIFESP]
Goulart, Ana Lucia [UNIFESP]
Santos, Amélia Miyashiro Nunes dos [UNIFESP]
author2_role author
author
author
author
author
author
dc.contributor.institution.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Perin, Maria Cristina Abrão Aued [UNIFESP]
Schlindwein, Carolina Frank [UNIFESP]
Moraes-Pinto, Maria Isabel de [UNIFESP]
Simao-Gurge, Raquel Maria [UNIFESP]
Mimica, Ana Flavia de Mello Almada [UNIFESP]
Goulart, Ana Lucia [UNIFESP]
Santos, Amélia Miyashiro Nunes dos [UNIFESP]
dc.subject.eng.fl_str_mv Premature infant
Immunization
Tetanus toxoid
Humoral immunity
Cellular immunity
topic Premature infant
Immunization
Tetanus toxoid
Humoral immunity
Cellular immunity
description Objectives: To compare humoral and cellular immune responses to tetanus booster vaccination in infants born prematurely with those born at full term and identify factors associated with the humoral response.Methods: A prospective study was carried out on children born prematurely and with a birth weight <1500g and with infants born at full term. At 15 months (pre-vaccination) and 18 months (post-vaccination), anti-tetanus antibodies were measured by ELISA; the intracellular interferon-gamma percentages of CD4+ T and CD8+ T cells after in vitro stimulation with tetanus toxoid were determined by flow cytometry. Chi-squared or Fisher's exact test was used to compare categorical variables. Student's t-test or Mann-Whitney test was used to compare numerical variables. Regression analysis was performed to determine factors associated with humoral immunity. Statistical significance was considered if p < 0.05.Results: Sixty-four premature and 54 full-term infants were studied. the proportion of children immune against tetanus at 15 and 18 months was similar in both groups. the geometric mean of the antibodies was lower among the premature children at 15 months (p = 0.025) and was similar in both groups at 18 months (p = 0.852). the percentages of CD4+ and CD8+ T cells expressing intracellular IFN-gamma were similar in both groups at 15 and 18 months. Gestational age <32 weeks was associated with a reduction of 0.116 IU/mL in the level of antibodies at 15 months. Breastfeeding >6 months was associated with a 3.5-fold greater chance of optimal protective (>= 0.1 IU/mL) antibody level against tetanus at 15 months and an increase of 0.956 in the level of antibodies at 18 months.Conclusions: Humoral and cellular response following a tetanus booster was similar in both groups. Premature infants exhibited lower levels of anti-tetanus antibodies at 15 months of age, with the lowest levels in those born at a gestational age of less than 32 weeks. Breastfeeding was associated with greater levels of antibody against tetanus. (C) 2012 Elsevier B.V. All rights reserved.
publishDate 2012
dc.date.issued.fl_str_mv 2012-10-12
dc.date.accessioned.fl_str_mv 2016-01-24T14:27:53Z
dc.date.available.fl_str_mv 2016-01-24T14:27:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv Vaccine. Oxford: Elsevier B.V., v. 30, n. 46, p. 6521-6526, 2012.
dc.identifier.uri.fl_str_mv http://repositorio.unifesp.br/handle/11600/35412
http://dx.doi.org/10.1016/j.vaccine.2012.08.056
dc.identifier.issn.none.fl_str_mv 0264-410X
dc.identifier.file.none.fl_str_mv WOS000310419700003.pdf
dc.identifier.doi.none.fl_str_mv 10.1016/j.vaccine.2012.08.056
dc.identifier.wos.none.fl_str_mv WOS:000310419700003
identifier_str_mv Vaccine. Oxford: Elsevier B.V., v. 30, n. 46, p. 6521-6526, 2012.
0264-410X
WOS000310419700003.pdf
10.1016/j.vaccine.2012.08.056
WOS:000310419700003
url http://repositorio.unifesp.br/handle/11600/35412
http://dx.doi.org/10.1016/j.vaccine.2012.08.056
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv Vaccine
dc.rights.driver.fl_str_mv http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
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rights_invalid_str_mv http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 6521-6526
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
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