Immune response to tetanus booster in infants aged 15 months born prematurely with very low birth weight
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/35412 http://dx.doi.org/10.1016/j.vaccine.2012.08.056 |
Resumo: | Objectives: To compare humoral and cellular immune responses to tetanus booster vaccination in infants born prematurely with those born at full term and identify factors associated with the humoral response.Methods: A prospective study was carried out on children born prematurely and with a birth weight <1500g and with infants born at full term. At 15 months (pre-vaccination) and 18 months (post-vaccination), anti-tetanus antibodies were measured by ELISA; the intracellular interferon-gamma percentages of CD4+ T and CD8+ T cells after in vitro stimulation with tetanus toxoid were determined by flow cytometry. Chi-squared or Fisher's exact test was used to compare categorical variables. Student's t-test or Mann-Whitney test was used to compare numerical variables. Regression analysis was performed to determine factors associated with humoral immunity. Statistical significance was considered if p < 0.05.Results: Sixty-four premature and 54 full-term infants were studied. the proportion of children immune against tetanus at 15 and 18 months was similar in both groups. the geometric mean of the antibodies was lower among the premature children at 15 months (p = 0.025) and was similar in both groups at 18 months (p = 0.852). the percentages of CD4+ and CD8+ T cells expressing intracellular IFN-gamma were similar in both groups at 15 and 18 months. Gestational age <32 weeks was associated with a reduction of 0.116 IU/mL in the level of antibodies at 15 months. Breastfeeding >6 months was associated with a 3.5-fold greater chance of optimal protective (>= 0.1 IU/mL) antibody level against tetanus at 15 months and an increase of 0.956 in the level of antibodies at 18 months.Conclusions: Humoral and cellular response following a tetanus booster was similar in both groups. Premature infants exhibited lower levels of anti-tetanus antibodies at 15 months of age, with the lowest levels in those born at a gestational age of less than 32 weeks. Breastfeeding was associated with greater levels of antibody against tetanus. (C) 2012 Elsevier B.V. All rights reserved. |
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Perin, Maria Cristina Abrão Aued [UNIFESP]Schlindwein, Carolina Frank [UNIFESP]Moraes-Pinto, Maria Isabel de [UNIFESP]Simao-Gurge, Raquel Maria [UNIFESP]Mimica, Ana Flavia de Mello Almada [UNIFESP]Goulart, Ana Lucia [UNIFESP]Santos, Amélia Miyashiro Nunes dos [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2016-01-24T14:27:53Z2016-01-24T14:27:53Z2012-10-12Vaccine. Oxford: Elsevier B.V., v. 30, n. 46, p. 6521-6526, 2012.0264-410Xhttp://repositorio.unifesp.br/handle/11600/35412http://dx.doi.org/10.1016/j.vaccine.2012.08.056WOS000310419700003.pdf10.1016/j.vaccine.2012.08.056WOS:000310419700003Objectives: To compare humoral and cellular immune responses to tetanus booster vaccination in infants born prematurely with those born at full term and identify factors associated with the humoral response.Methods: A prospective study was carried out on children born prematurely and with a birth weight <1500g and with infants born at full term. At 15 months (pre-vaccination) and 18 months (post-vaccination), anti-tetanus antibodies were measured by ELISA; the intracellular interferon-gamma percentages of CD4+ T and CD8+ T cells after in vitro stimulation with tetanus toxoid were determined by flow cytometry. Chi-squared or Fisher's exact test was used to compare categorical variables. Student's t-test or Mann-Whitney test was used to compare numerical variables. Regression analysis was performed to determine factors associated with humoral immunity. Statistical significance was considered if p < 0.05.Results: Sixty-four premature and 54 full-term infants were studied. the proportion of children immune against tetanus at 15 and 18 months was similar in both groups. the geometric mean of the antibodies was lower among the premature children at 15 months (p = 0.025) and was similar in both groups at 18 months (p = 0.852). the percentages of CD4+ and CD8+ T cells expressing intracellular IFN-gamma were similar in both groups at 15 and 18 months. Gestational age <32 weeks was associated with a reduction of 0.116 IU/mL in the level of antibodies at 15 months. Breastfeeding >6 months was associated with a 3.5-fold greater chance of optimal protective (>= 0.1 IU/mL) antibody level against tetanus at 15 months and an increase of 0.956 in the level of antibodies at 18 months.Conclusions: Humoral and cellular response following a tetanus booster was similar in both groups. Premature infants exhibited lower levels of anti-tetanus antibodies at 15 months of age, with the lowest levels in those born at a gestational age of less than 32 weeks. Breastfeeding was associated with greater levels of antibody against tetanus. (C) 2012 Elsevier B.V. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Federal de São Paulo, Neonatal Div Med, Dept Pediat, São Paulo, BrazilUniversidade Federal de São Paulo, Div Pediat Infect Dis, Dept Pediat, São Paulo, BrazilUniversidade Federal de São Paulo, Neonatal Div Med, Dept Pediat, São Paulo, BrazilUniversidade Federal de São Paulo, Div Pediat Infect Dis, Dept Pediat, São Paulo, BrazilFAPESP: 06/51865-8FAPESP: 09/14351-4Web of Science6521-6526engElsevier B.V.Vaccinehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyinfo:eu-repo/semantics/openAccessPremature infantImmunizationTetanus toxoidHumoral immunityCellular immunityImmune response to tetanus booster in infants aged 15 months born prematurely with very low birth weightinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000310419700003.pdfapplication/pdf262359${dspace.ui.url}/bitstream/11600/35412/1/WOS000310419700003.pdfe37a84b04d7cef10d0330a113b7d9d08MD51open accessTEXTWOS000310419700003.pdf.txtWOS000310419700003.pdf.txtExtracted texttext/plain37277${dspace.ui.url}/bitstream/11600/35412/2/WOS000310419700003.pdf.txte6fb1e53b14aa6d58ef8b6a705554e1cMD52open access11600/354122022-06-02 09:34:58.742open accessoai:repositorio.unifesp.br:11600/35412Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:16:16.947130Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Immune response to tetanus booster in infants aged 15 months born prematurely with very low birth weight |
title |
Immune response to tetanus booster in infants aged 15 months born prematurely with very low birth weight |
spellingShingle |
Immune response to tetanus booster in infants aged 15 months born prematurely with very low birth weight Perin, Maria Cristina Abrão Aued [UNIFESP] Premature infant Immunization Tetanus toxoid Humoral immunity Cellular immunity |
title_short |
Immune response to tetanus booster in infants aged 15 months born prematurely with very low birth weight |
title_full |
Immune response to tetanus booster in infants aged 15 months born prematurely with very low birth weight |
title_fullStr |
Immune response to tetanus booster in infants aged 15 months born prematurely with very low birth weight |
title_full_unstemmed |
Immune response to tetanus booster in infants aged 15 months born prematurely with very low birth weight |
title_sort |
Immune response to tetanus booster in infants aged 15 months born prematurely with very low birth weight |
author |
Perin, Maria Cristina Abrão Aued [UNIFESP] |
author_facet |
Perin, Maria Cristina Abrão Aued [UNIFESP] Schlindwein, Carolina Frank [UNIFESP] Moraes-Pinto, Maria Isabel de [UNIFESP] Simao-Gurge, Raquel Maria [UNIFESP] Mimica, Ana Flavia de Mello Almada [UNIFESP] Goulart, Ana Lucia [UNIFESP] Santos, Amélia Miyashiro Nunes dos [UNIFESP] |
author_role |
author |
author2 |
Schlindwein, Carolina Frank [UNIFESP] Moraes-Pinto, Maria Isabel de [UNIFESP] Simao-Gurge, Raquel Maria [UNIFESP] Mimica, Ana Flavia de Mello Almada [UNIFESP] Goulart, Ana Lucia [UNIFESP] Santos, Amélia Miyashiro Nunes dos [UNIFESP] |
author2_role |
author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Perin, Maria Cristina Abrão Aued [UNIFESP] Schlindwein, Carolina Frank [UNIFESP] Moraes-Pinto, Maria Isabel de [UNIFESP] Simao-Gurge, Raquel Maria [UNIFESP] Mimica, Ana Flavia de Mello Almada [UNIFESP] Goulart, Ana Lucia [UNIFESP] Santos, Amélia Miyashiro Nunes dos [UNIFESP] |
dc.subject.eng.fl_str_mv |
Premature infant Immunization Tetanus toxoid Humoral immunity Cellular immunity |
topic |
Premature infant Immunization Tetanus toxoid Humoral immunity Cellular immunity |
description |
Objectives: To compare humoral and cellular immune responses to tetanus booster vaccination in infants born prematurely with those born at full term and identify factors associated with the humoral response.Methods: A prospective study was carried out on children born prematurely and with a birth weight <1500g and with infants born at full term. At 15 months (pre-vaccination) and 18 months (post-vaccination), anti-tetanus antibodies were measured by ELISA; the intracellular interferon-gamma percentages of CD4+ T and CD8+ T cells after in vitro stimulation with tetanus toxoid were determined by flow cytometry. Chi-squared or Fisher's exact test was used to compare categorical variables. Student's t-test or Mann-Whitney test was used to compare numerical variables. Regression analysis was performed to determine factors associated with humoral immunity. Statistical significance was considered if p < 0.05.Results: Sixty-four premature and 54 full-term infants were studied. the proportion of children immune against tetanus at 15 and 18 months was similar in both groups. the geometric mean of the antibodies was lower among the premature children at 15 months (p = 0.025) and was similar in both groups at 18 months (p = 0.852). the percentages of CD4+ and CD8+ T cells expressing intracellular IFN-gamma were similar in both groups at 15 and 18 months. Gestational age <32 weeks was associated with a reduction of 0.116 IU/mL in the level of antibodies at 15 months. Breastfeeding >6 months was associated with a 3.5-fold greater chance of optimal protective (>= 0.1 IU/mL) antibody level against tetanus at 15 months and an increase of 0.956 in the level of antibodies at 18 months.Conclusions: Humoral and cellular response following a tetanus booster was similar in both groups. Premature infants exhibited lower levels of anti-tetanus antibodies at 15 months of age, with the lowest levels in those born at a gestational age of less than 32 weeks. Breastfeeding was associated with greater levels of antibody against tetanus. (C) 2012 Elsevier B.V. All rights reserved. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-10-12 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:27:53Z |
dc.date.available.fl_str_mv |
2016-01-24T14:27:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Vaccine. Oxford: Elsevier B.V., v. 30, n. 46, p. 6521-6526, 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/35412 http://dx.doi.org/10.1016/j.vaccine.2012.08.056 |
dc.identifier.issn.none.fl_str_mv |
0264-410X |
dc.identifier.file.none.fl_str_mv |
WOS000310419700003.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.vaccine.2012.08.056 |
dc.identifier.wos.none.fl_str_mv |
WOS:000310419700003 |
identifier_str_mv |
Vaccine. Oxford: Elsevier B.V., v. 30, n. 46, p. 6521-6526, 2012. 0264-410X WOS000310419700003.pdf 10.1016/j.vaccine.2012.08.056 WOS:000310419700003 |
url |
http://repositorio.unifesp.br/handle/11600/35412 http://dx.doi.org/10.1016/j.vaccine.2012.08.056 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Vaccine |
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http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
6521-6526 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
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Universidade Federal de São Paulo (UNIFESP) |
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