Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo city

Detalhes bibliográficos
Autor(a) principal: Teixeira, Daniela [UNIFESP]
Data de Publicação: 2011
Outros Autores: Longo-Maugéri, Ieda Maria [UNIFESP], Santos, Jair Licio Ferreira, Duarte, Yeda Aparecida Oliveira, Lebrão, Maria Lucia, Bueno, Valquiria [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.5581/1516-8484.20110100
http://repositorio.unifesp.br/handle/11600/6672
Resumo: BACKGROUND: Age-associated changes in the immune system cause decreased protection after vaccination and increased rates of infections and tumor development. METHODS: Lymphocyte percentages were compared by gender and age to establish differences between subtypes. Three mL blood samples were obtained from 218 randomly selected individuals (60-101 years old) who live in São Paulo city. Blood was lysed with Tris phosphate buffer and stained for 30 minutes with monoclonal antibodies (CD3PerCP, CD4FITC, CD8Pe, CD19Pe) for analysis by flow cytometry. Statistical analysis was by ANOVA. RESULTS: The percentage of CD4+ T cells (p-value = 0.005) and the CD4/CD8 ratio (p-value = 0.010) were lower in men, whereas the percentage of CD8+ T cells was lower (p-value = 0.002) in women; the percentage of B cells (CD19+ ) was similar between groups. Individuals grouped by gender and age range and compared showed a drop in CD4+ cells in 75 to 79-year-old men (female: 46.1% ± 8.1% and male: 38.8% ± 10.5%; p-value = 0.023). Also, the 80 to 84-year-old group of men had a higher percentage of CD8+ (female: 20.8% ± 8.2%, and male: 27.2% ± 8.2%; p-value = 0.032). Low percentages of B cells were detected in men in the 75 to 79-year-old (p-value = 0.003), 85 to 89-year-old (p-value = 0.020) and older than 90 year old (p-value = 0.002) age ranges. CONCLUSION: Elderly men present with more changes in lymphocyte subsets compared to elderly women. These findings could demonstrate impairment in the immune response since the lower CD4+ in men would provide less help to B cells (also lower in men) in terms of antibody production. In addition, the increase in CD8+ cells in this group could represent chronic inflammation observed during the aging process.
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spelling Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo cityAgingSex distributionImmune SystemLymphocytesFlow CytometryBACKGROUND: Age-associated changes in the immune system cause decreased protection after vaccination and increased rates of infections and tumor development. METHODS: Lymphocyte percentages were compared by gender and age to establish differences between subtypes. Three mL blood samples were obtained from 218 randomly selected individuals (60-101 years old) who live in São Paulo city. Blood was lysed with Tris phosphate buffer and stained for 30 minutes with monoclonal antibodies (CD3PerCP, CD4FITC, CD8Pe, CD19Pe) for analysis by flow cytometry. Statistical analysis was by ANOVA. RESULTS: The percentage of CD4+ T cells (p-value = 0.005) and the CD4/CD8 ratio (p-value = 0.010) were lower in men, whereas the percentage of CD8+ T cells was lower (p-value = 0.002) in women; the percentage of B cells (CD19+ ) was similar between groups. Individuals grouped by gender and age range and compared showed a drop in CD4+ cells in 75 to 79-year-old men (female: 46.1% ± 8.1% and male: 38.8% ± 10.5%; p-value = 0.023). Also, the 80 to 84-year-old group of men had a higher percentage of CD8+ (female: 20.8% ± 8.2%, and male: 27.2% ± 8.2%; p-value = 0.032). Low percentages of B cells were detected in men in the 75 to 79-year-old (p-value = 0.003), 85 to 89-year-old (p-value = 0.020) and older than 90 year old (p-value = 0.002) age ranges. CONCLUSION: Elderly men present with more changes in lymphocyte subsets compared to elderly women. These findings could demonstrate impairment in the immune response since the lower CD4+ in men would provide less help to B cells (also lower in men) in terms of antibody production. In addition, the increase in CD8+ cells in this group could represent chronic inflammation observed during the aging process.Universidade Federal de São Paulo (UNIFESP) Immunology DepartmentUniversidade de São Paulo Faculdade de Medicina de Ribeirão PretoUniversidade de São Paulo Escola de Saúde PúblicaUniversidade Federal de São Paulo (UNIFESP) Biosciences DepartmentUNIFESP, Immunology DepartmentUNIFESP, Biosciences DepartmentSciELOFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Associação Brasileira de Hematologia e Hemoterapia e Terapia CelularUniversidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Teixeira, Daniela [UNIFESP]Longo-Maugéri, Ieda Maria [UNIFESP]Santos, Jair Licio FerreiraDuarte, Yeda Aparecida OliveiraLebrão, Maria LuciaBueno, Valquiria [UNIFESP]2015-06-14T13:43:19Z2015-06-14T13:43:19Z2011-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion367-371application/pdfhttp://dx.doi.org/10.5581/1516-8484.20110100Revista Brasileira de Hematologia e Hemoterapia. Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular, v. 33, n. 5, p. 367-371, 2011.10.5581/1516-8484.20110100S1516-84842011000500013.pdf1516-8484S1516-84842011000500013http://repositorio.unifesp.br/handle/11600/6672engRevista Brasileira de Hematologia e Hemoterapiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-30T01:49:15Zoai:repositorio.unifesp.br/:11600/6672Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-30T01:49:15Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo city
title Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo city
spellingShingle Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo city
Teixeira, Daniela [UNIFESP]
Aging
Sex distribution
Immune System
Lymphocytes
Flow Cytometry
title_short Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo city
title_full Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo city
title_fullStr Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo city
title_full_unstemmed Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo city
title_sort Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo city
author Teixeira, Daniela [UNIFESP]
author_facet Teixeira, Daniela [UNIFESP]
Longo-Maugéri, Ieda Maria [UNIFESP]
Santos, Jair Licio Ferreira
Duarte, Yeda Aparecida Oliveira
Lebrão, Maria Lucia
Bueno, Valquiria [UNIFESP]
author_role author
author2 Longo-Maugéri, Ieda Maria [UNIFESP]
Santos, Jair Licio Ferreira
Duarte, Yeda Aparecida Oliveira
Lebrão, Maria Lucia
Bueno, Valquiria [UNIFESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Teixeira, Daniela [UNIFESP]
Longo-Maugéri, Ieda Maria [UNIFESP]
Santos, Jair Licio Ferreira
Duarte, Yeda Aparecida Oliveira
Lebrão, Maria Lucia
Bueno, Valquiria [UNIFESP]
dc.subject.por.fl_str_mv Aging
Sex distribution
Immune System
Lymphocytes
Flow Cytometry
topic Aging
Sex distribution
Immune System
Lymphocytes
Flow Cytometry
description BACKGROUND: Age-associated changes in the immune system cause decreased protection after vaccination and increased rates of infections and tumor development. METHODS: Lymphocyte percentages were compared by gender and age to establish differences between subtypes. Three mL blood samples were obtained from 218 randomly selected individuals (60-101 years old) who live in São Paulo city. Blood was lysed with Tris phosphate buffer and stained for 30 minutes with monoclonal antibodies (CD3PerCP, CD4FITC, CD8Pe, CD19Pe) for analysis by flow cytometry. Statistical analysis was by ANOVA. RESULTS: The percentage of CD4+ T cells (p-value = 0.005) and the CD4/CD8 ratio (p-value = 0.010) were lower in men, whereas the percentage of CD8+ T cells was lower (p-value = 0.002) in women; the percentage of B cells (CD19+ ) was similar between groups. Individuals grouped by gender and age range and compared showed a drop in CD4+ cells in 75 to 79-year-old men (female: 46.1% ± 8.1% and male: 38.8% ± 10.5%; p-value = 0.023). Also, the 80 to 84-year-old group of men had a higher percentage of CD8+ (female: 20.8% ± 8.2%, and male: 27.2% ± 8.2%; p-value = 0.032). Low percentages of B cells were detected in men in the 75 to 79-year-old (p-value = 0.003), 85 to 89-year-old (p-value = 0.020) and older than 90 year old (p-value = 0.002) age ranges. CONCLUSION: Elderly men present with more changes in lymphocyte subsets compared to elderly women. These findings could demonstrate impairment in the immune response since the lower CD4+ in men would provide less help to B cells (also lower in men) in terms of antibody production. In addition, the increase in CD8+ cells in this group could represent chronic inflammation observed during the aging process.
publishDate 2011
dc.date.none.fl_str_mv 2011-10-01
2015-06-14T13:43:19Z
2015-06-14T13:43:19Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.5581/1516-8484.20110100
Revista Brasileira de Hematologia e Hemoterapia. Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular, v. 33, n. 5, p. 367-371, 2011.
10.5581/1516-8484.20110100
S1516-84842011000500013.pdf
1516-8484
S1516-84842011000500013
http://repositorio.unifesp.br/handle/11600/6672
url http://dx.doi.org/10.5581/1516-8484.20110100
http://repositorio.unifesp.br/handle/11600/6672
identifier_str_mv Revista Brasileira de Hematologia e Hemoterapia. Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular, v. 33, n. 5, p. 367-371, 2011.
10.5581/1516-8484.20110100
S1516-84842011000500013.pdf
1516-8484
S1516-84842011000500013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Brasileira de Hematologia e Hemoterapia
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 367-371
application/pdf
dc.publisher.none.fl_str_mv Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular
publisher.none.fl_str_mv Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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