Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo city
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.5581/1516-8484.20110100 http://repositorio.unifesp.br/handle/11600/6672 |
Resumo: | BACKGROUND: Age-associated changes in the immune system cause decreased protection after vaccination and increased rates of infections and tumor development. METHODS: Lymphocyte percentages were compared by gender and age to establish differences between subtypes. Three mL blood samples were obtained from 218 randomly selected individuals (60-101 years old) who live in São Paulo city. Blood was lysed with Tris phosphate buffer and stained for 30 minutes with monoclonal antibodies (CD3PerCP, CD4FITC, CD8Pe, CD19Pe) for analysis by flow cytometry. Statistical analysis was by ANOVA. RESULTS: The percentage of CD4+ T cells (p-value = 0.005) and the CD4/CD8 ratio (p-value = 0.010) were lower in men, whereas the percentage of CD8+ T cells was lower (p-value = 0.002) in women; the percentage of B cells (CD19+ ) was similar between groups. Individuals grouped by gender and age range and compared showed a drop in CD4+ cells in 75 to 79-year-old men (female: 46.1% ± 8.1% and male: 38.8% ± 10.5%; p-value = 0.023). Also, the 80 to 84-year-old group of men had a higher percentage of CD8+ (female: 20.8% ± 8.2%, and male: 27.2% ± 8.2%; p-value = 0.032). Low percentages of B cells were detected in men in the 75 to 79-year-old (p-value = 0.003), 85 to 89-year-old (p-value = 0.020) and older than 90 year old (p-value = 0.002) age ranges. CONCLUSION: Elderly men present with more changes in lymphocyte subsets compared to elderly women. These findings could demonstrate impairment in the immune response since the lower CD4+ in men would provide less help to B cells (also lower in men) in terms of antibody production. In addition, the increase in CD8+ cells in this group could represent chronic inflammation observed during the aging process. |
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Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo cityAgingSex distributionImmune SystemLymphocytesFlow CytometryBACKGROUND: Age-associated changes in the immune system cause decreased protection after vaccination and increased rates of infections and tumor development. METHODS: Lymphocyte percentages were compared by gender and age to establish differences between subtypes. Three mL blood samples were obtained from 218 randomly selected individuals (60-101 years old) who live in São Paulo city. Blood was lysed with Tris phosphate buffer and stained for 30 minutes with monoclonal antibodies (CD3PerCP, CD4FITC, CD8Pe, CD19Pe) for analysis by flow cytometry. Statistical analysis was by ANOVA. RESULTS: The percentage of CD4+ T cells (p-value = 0.005) and the CD4/CD8 ratio (p-value = 0.010) were lower in men, whereas the percentage of CD8+ T cells was lower (p-value = 0.002) in women; the percentage of B cells (CD19+ ) was similar between groups. Individuals grouped by gender and age range and compared showed a drop in CD4+ cells in 75 to 79-year-old men (female: 46.1% ± 8.1% and male: 38.8% ± 10.5%; p-value = 0.023). Also, the 80 to 84-year-old group of men had a higher percentage of CD8+ (female: 20.8% ± 8.2%, and male: 27.2% ± 8.2%; p-value = 0.032). Low percentages of B cells were detected in men in the 75 to 79-year-old (p-value = 0.003), 85 to 89-year-old (p-value = 0.020) and older than 90 year old (p-value = 0.002) age ranges. CONCLUSION: Elderly men present with more changes in lymphocyte subsets compared to elderly women. These findings could demonstrate impairment in the immune response since the lower CD4+ in men would provide less help to B cells (also lower in men) in terms of antibody production. In addition, the increase in CD8+ cells in this group could represent chronic inflammation observed during the aging process.Universidade Federal de São Paulo (UNIFESP) Immunology DepartmentUniversidade de São Paulo Faculdade de Medicina de Ribeirão PretoUniversidade de São Paulo Escola de Saúde PúblicaUniversidade Federal de São Paulo (UNIFESP) Biosciences DepartmentUNIFESP, Immunology DepartmentUNIFESP, Biosciences DepartmentSciELOFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Associação Brasileira de Hematologia e Hemoterapia e Terapia CelularUniversidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Teixeira, Daniela [UNIFESP]Longo-Maugéri, Ieda Maria [UNIFESP]Santos, Jair Licio FerreiraDuarte, Yeda Aparecida OliveiraLebrão, Maria LuciaBueno, Valquiria [UNIFESP]2015-06-14T13:43:19Z2015-06-14T13:43:19Z2011-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion367-371application/pdfhttp://dx.doi.org/10.5581/1516-8484.20110100Revista Brasileira de Hematologia e Hemoterapia. Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular, v. 33, n. 5, p. 367-371, 2011.10.5581/1516-8484.20110100S1516-84842011000500013.pdf1516-8484S1516-84842011000500013http://repositorio.unifesp.br/handle/11600/6672engRevista Brasileira de Hematologia e Hemoterapiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-30T01:49:15Zoai:repositorio.unifesp.br/:11600/6672Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-30T01:49:15Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo city |
title |
Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo city |
spellingShingle |
Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo city Teixeira, Daniela [UNIFESP] Aging Sex distribution Immune System Lymphocytes Flow Cytometry |
title_short |
Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo city |
title_full |
Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo city |
title_fullStr |
Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo city |
title_full_unstemmed |
Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo city |
title_sort |
Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo city |
author |
Teixeira, Daniela [UNIFESP] |
author_facet |
Teixeira, Daniela [UNIFESP] Longo-Maugéri, Ieda Maria [UNIFESP] Santos, Jair Licio Ferreira Duarte, Yeda Aparecida Oliveira Lebrão, Maria Lucia Bueno, Valquiria [UNIFESP] |
author_role |
author |
author2 |
Longo-Maugéri, Ieda Maria [UNIFESP] Santos, Jair Licio Ferreira Duarte, Yeda Aparecida Oliveira Lebrão, Maria Lucia Bueno, Valquiria [UNIFESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Teixeira, Daniela [UNIFESP] Longo-Maugéri, Ieda Maria [UNIFESP] Santos, Jair Licio Ferreira Duarte, Yeda Aparecida Oliveira Lebrão, Maria Lucia Bueno, Valquiria [UNIFESP] |
dc.subject.por.fl_str_mv |
Aging Sex distribution Immune System Lymphocytes Flow Cytometry |
topic |
Aging Sex distribution Immune System Lymphocytes Flow Cytometry |
description |
BACKGROUND: Age-associated changes in the immune system cause decreased protection after vaccination and increased rates of infections and tumor development. METHODS: Lymphocyte percentages were compared by gender and age to establish differences between subtypes. Three mL blood samples were obtained from 218 randomly selected individuals (60-101 years old) who live in São Paulo city. Blood was lysed with Tris phosphate buffer and stained for 30 minutes with monoclonal antibodies (CD3PerCP, CD4FITC, CD8Pe, CD19Pe) for analysis by flow cytometry. Statistical analysis was by ANOVA. RESULTS: The percentage of CD4+ T cells (p-value = 0.005) and the CD4/CD8 ratio (p-value = 0.010) were lower in men, whereas the percentage of CD8+ T cells was lower (p-value = 0.002) in women; the percentage of B cells (CD19+ ) was similar between groups. Individuals grouped by gender and age range and compared showed a drop in CD4+ cells in 75 to 79-year-old men (female: 46.1% ± 8.1% and male: 38.8% ± 10.5%; p-value = 0.023). Also, the 80 to 84-year-old group of men had a higher percentage of CD8+ (female: 20.8% ± 8.2%, and male: 27.2% ± 8.2%; p-value = 0.032). Low percentages of B cells were detected in men in the 75 to 79-year-old (p-value = 0.003), 85 to 89-year-old (p-value = 0.020) and older than 90 year old (p-value = 0.002) age ranges. CONCLUSION: Elderly men present with more changes in lymphocyte subsets compared to elderly women. These findings could demonstrate impairment in the immune response since the lower CD4+ in men would provide less help to B cells (also lower in men) in terms of antibody production. In addition, the increase in CD8+ cells in this group could represent chronic inflammation observed during the aging process. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-10-01 2015-06-14T13:43:19Z 2015-06-14T13:43:19Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.5581/1516-8484.20110100 Revista Brasileira de Hematologia e Hemoterapia. Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular, v. 33, n. 5, p. 367-371, 2011. 10.5581/1516-8484.20110100 S1516-84842011000500013.pdf 1516-8484 S1516-84842011000500013 http://repositorio.unifesp.br/handle/11600/6672 |
url |
http://dx.doi.org/10.5581/1516-8484.20110100 http://repositorio.unifesp.br/handle/11600/6672 |
identifier_str_mv |
Revista Brasileira de Hematologia e Hemoterapia. Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular, v. 33, n. 5, p. 367-371, 2011. 10.5581/1516-8484.20110100 S1516-84842011000500013.pdf 1516-8484 S1516-84842011000500013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira de Hematologia e Hemoterapia |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
367-371 application/pdf |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular |
publisher.none.fl_str_mv |
Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268312698224640 |