Comparative phenotypic and genomic features of Staphylococci from sonication fluid of orthopedic implant-associated infections with poor outcome
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://doi.org/10.3390/microorganisms10061149 https://repositorio.unifesp.br/xmlui/handle/11600/63955 |
Resumo: | Staphylococcus spp. remain the leading biofilm-forming agents causing orthopedic im-plant-associated infections (OIAI). This is a descriptive study of phenotypic and genomic features identified in clinical isolates of S. aureus and coagulase-negative Staphylococcus (CoNS) recovered from OIAIs patients that progressed to treatment failure. Ten isolates were identified by matrix-time-of-flight laser-assisted desorption mass spectrometry (MALDI-TOF-MS) and tested for antibi-otic susceptibility and biofilm formation. Genotypic characteristics, including, MLST (Multi Locus Sequence Typing), SCCmec typing, virulence and resistance genes were assessed by whole-genome sequencing (WGS). All S. aureus harbored mecA, blaZ, and multiple resistance genes for aminogly-cosides and quinolones. All MRSA were strong biofilm producers harboring the complete icaADBC and icaR operon. Seven CoNS isolates comprising five species (S. epidermidis, S. haemolyticus, S. sci-uri, S. capitis and S. lugdunensis) were analyzed, with mecA gene detected in five isolates. S. haemoli-tycus (isolate 95), and S. lugdunensis were unable to form biofilm and did not harbor the complete icaADBCR operon. High variability of adhesion genes was detected, with atl, ebp, icaADBC operon, and IS256 being the most common. In conclusion, MRSA and CoNS isolates carrying genes for bio-film production, and resistance to β-lactam and aminoglycosides are associated with treatment fail-ure in OIAIs. |
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Santos, Ingrid Nayara Marcelino [UNIFESP]Kurihara, Mariana Neri Lucas [UNIFESP]Santos, Fernanda FernandesValiatti, Tiago Barcelos [UNIFESP]Silva, Juliana Thalita Paulino [UNIFESP]Pignatari, Antonio Carlos Campos [UNIFESP]Salles, Mauro Jose Costa [UNIFESP]http://lattes.cnpq.br/7196518766323325http://lattes.cnpq.br/0214364156717092http://lattes.cnpq.br/6922450552975380http://lattes.cnpq.br/1570873280199853http://lattes.cnpq.br/9461346610553865http://lattes.cnpq.br/31199338645204842022-06-09T17:44:39Z2022-06-09T17:44:39Z2022-06-02https://doi.org/10.3390/microorganisms10061149https://repositorio.unifesp.br/xmlui/handle/11600/6395510.3390/microorganisms10061149Staphylococcus spp. remain the leading biofilm-forming agents causing orthopedic im-plant-associated infections (OIAI). This is a descriptive study of phenotypic and genomic features identified in clinical isolates of S. aureus and coagulase-negative Staphylococcus (CoNS) recovered from OIAIs patients that progressed to treatment failure. Ten isolates were identified by matrix-time-of-flight laser-assisted desorption mass spectrometry (MALDI-TOF-MS) and tested for antibi-otic susceptibility and biofilm formation. Genotypic characteristics, including, MLST (Multi Locus Sequence Typing), SCCmec typing, virulence and resistance genes were assessed by whole-genome sequencing (WGS). All S. aureus harbored mecA, blaZ, and multiple resistance genes for aminogly-cosides and quinolones. All MRSA were strong biofilm producers harboring the complete icaADBC and icaR operon. Seven CoNS isolates comprising five species (S. epidermidis, S. haemolyticus, S. sci-uri, S. capitis and S. lugdunensis) were analyzed, with mecA gene detected in five isolates. S. haemoli-tycus (isolate 95), and S. lugdunensis were unable to form biofilm and did not harbor the complete icaADBCR operon. High variability of adhesion genes was detected, with atl, ebp, icaADBC operon, and IS256 being the most common. In conclusion, MRSA and CoNS isolates carrying genes for bio-film production, and resistance to β-lactam and aminoglycosides are associated with treatment fail-ure in OIAIs.engMDPIMicroorganismsbacterial resistancewhole-genome sequencingStaphylococcus aureuscoagulase-negative StaphylococciBiofilmOrthopedic infectionsSonicationComparative phenotypic and genomic features of Staphylococci from sonication fluid of orthopedic implant-associated infections with poor outcomeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPEscola Paulista de Medicina (EPM)InfectologiaDoenças infecciosas e parasitáriasInfecções Associadas aos BiofilmesMedicinaBiomedicinaORIGINALartigo do mestrado.pdfartigo do mestrado.pdfapplication/pdf596098${dspace.ui.url}/bitstream/11600/63955/1/artigo%20do%20mestrado.pdfe8d8e0d6dda050b48cad1623c262cc9bMD51open 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InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-16T04:46:06Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Comparative phenotypic and genomic features of Staphylococci from sonication fluid of orthopedic implant-associated infections with poor outcome |
title |
Comparative phenotypic and genomic features of Staphylococci from sonication fluid of orthopedic implant-associated infections with poor outcome |
spellingShingle |
Comparative phenotypic and genomic features of Staphylococci from sonication fluid of orthopedic implant-associated infections with poor outcome Santos, Ingrid Nayara Marcelino [UNIFESP] bacterial resistance whole-genome sequencing Staphylococcus aureus coagulase-negative Staphylococci Biofilm Orthopedic infections Sonication |
title_short |
Comparative phenotypic and genomic features of Staphylococci from sonication fluid of orthopedic implant-associated infections with poor outcome |
title_full |
Comparative phenotypic and genomic features of Staphylococci from sonication fluid of orthopedic implant-associated infections with poor outcome |
title_fullStr |
Comparative phenotypic and genomic features of Staphylococci from sonication fluid of orthopedic implant-associated infections with poor outcome |
title_full_unstemmed |
Comparative phenotypic and genomic features of Staphylococci from sonication fluid of orthopedic implant-associated infections with poor outcome |
title_sort |
Comparative phenotypic and genomic features of Staphylococci from sonication fluid of orthopedic implant-associated infections with poor outcome |
author |
Santos, Ingrid Nayara Marcelino [UNIFESP] |
author_facet |
Santos, Ingrid Nayara Marcelino [UNIFESP] Kurihara, Mariana Neri Lucas [UNIFESP] Santos, Fernanda Fernandes Valiatti, Tiago Barcelos [UNIFESP] Silva, Juliana Thalita Paulino [UNIFESP] Pignatari, Antonio Carlos Campos [UNIFESP] Salles, Mauro Jose Costa [UNIFESP] |
author_role |
author |
author2 |
Kurihara, Mariana Neri Lucas [UNIFESP] Santos, Fernanda Fernandes Valiatti, Tiago Barcelos [UNIFESP] Silva, Juliana Thalita Paulino [UNIFESP] Pignatari, Antonio Carlos Campos [UNIFESP] Salles, Mauro Jose Costa [UNIFESP] |
author2_role |
author author author author author author |
dc.contributor.authorLattes.pt_BR.fl_str_mv |
http://lattes.cnpq.br/7196518766323325 |
dc.contributor.authorLattes.none.fl_str_mv |
http://lattes.cnpq.br/0214364156717092 http://lattes.cnpq.br/6922450552975380 http://lattes.cnpq.br/1570873280199853 http://lattes.cnpq.br/9461346610553865 http://lattes.cnpq.br/3119933864520484 |
dc.contributor.author.fl_str_mv |
Santos, Ingrid Nayara Marcelino [UNIFESP] Kurihara, Mariana Neri Lucas [UNIFESP] Santos, Fernanda Fernandes Valiatti, Tiago Barcelos [UNIFESP] Silva, Juliana Thalita Paulino [UNIFESP] Pignatari, Antonio Carlos Campos [UNIFESP] Salles, Mauro Jose Costa [UNIFESP] |
dc.subject.por.fl_str_mv |
bacterial resistance whole-genome sequencing |
topic |
bacterial resistance whole-genome sequencing Staphylococcus aureus coagulase-negative Staphylococci Biofilm Orthopedic infections Sonication |
dc.subject.eng.fl_str_mv |
Staphylococcus aureus coagulase-negative Staphylococci Biofilm Orthopedic infections Sonication |
description |
Staphylococcus spp. remain the leading biofilm-forming agents causing orthopedic im-plant-associated infections (OIAI). This is a descriptive study of phenotypic and genomic features identified in clinical isolates of S. aureus and coagulase-negative Staphylococcus (CoNS) recovered from OIAIs patients that progressed to treatment failure. Ten isolates were identified by matrix-time-of-flight laser-assisted desorption mass spectrometry (MALDI-TOF-MS) and tested for antibi-otic susceptibility and biofilm formation. Genotypic characteristics, including, MLST (Multi Locus Sequence Typing), SCCmec typing, virulence and resistance genes were assessed by whole-genome sequencing (WGS). All S. aureus harbored mecA, blaZ, and multiple resistance genes for aminogly-cosides and quinolones. All MRSA were strong biofilm producers harboring the complete icaADBC and icaR operon. Seven CoNS isolates comprising five species (S. epidermidis, S. haemolyticus, S. sci-uri, S. capitis and S. lugdunensis) were analyzed, with mecA gene detected in five isolates. S. haemoli-tycus (isolate 95), and S. lugdunensis were unable to form biofilm and did not harbor the complete icaADBCR operon. High variability of adhesion genes was detected, with atl, ebp, icaADBC operon, and IS256 being the most common. In conclusion, MRSA and CoNS isolates carrying genes for bio-film production, and resistance to β-lactam and aminoglycosides are associated with treatment fail-ure in OIAIs. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-06-09T17:44:39Z |
dc.date.available.fl_str_mv |
2022-06-09T17:44:39Z |
dc.date.issued.fl_str_mv |
2022-06-02 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.pt_BR.fl_str_mv |
https://doi.org/10.3390/microorganisms10061149 |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/xmlui/handle/11600/63955 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.3390/microorganisms10061149 |
url |
https://doi.org/10.3390/microorganisms10061149 https://repositorio.unifesp.br/xmlui/handle/11600/63955 |
identifier_str_mv |
10.3390/microorganisms10061149 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.en.fl_str_mv |
Microorganisms |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
bitstream.url.fl_str_mv |
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MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
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1802764259077128192 |