cagA positive Helicobacter pylori in Brazilian children related to chronic gastritis

Detalhes bibliográficos
Autor(a) principal: Gatti, Luciano Lobo [UNIFESP]
Data de Publicação: 2006
Outros Autores: Lábio, Roger Willian de, Silva, Luiz Carlos da, Smith, Marilia de Arruda Cardoso [UNIFESP], Payão, Spencer Luiz Marques [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S1413-86702006000400008
http://repositorio.unifesp.br/handle/11600/3174
Resumo: Helicobacter pylori is a spiral-shaped Gram-negative bacterium. It colonizes the gastric mucosa of humans and persists for decades if not treated. Helicobacter pylori infection affects more than half of the world's population and invariably results in chronic gastritis. The cagA gene is present in about 60 to 70% of H. pylori strains; it encodes a high-molecular-weight protein (120 to 140 kDa) and several investigators have noted a correlation between strains that possess cagA and the severity of gastric mucosal inflammation. We examined the relation between cagA status in H. pylori strains and chronic gastritis with inflammatory processes in children from Marília, São Paulo, Brazil. One-hundred-twenty-one children were analyzed histopathologically and by polymerase chain reaction (PCR) to detect H. pylori and cagA. We then looked for an association between cagA presence and inflammatory infiltration. Using histology and PCR, we found 47% H. pylori positive infection; 29 children were diagnosed with chronic gastritis, while 28 showed normal mucosa by histopathological analysis. CagA presence was genotyped in both groups, and an inflammatory infiltrate was studied in all infected children with chronic gastritis. We found cagA strains in 20 of 29 (69%) children with chronic gastritis and 18 of 28 (64%) with normal mucosa, demonstrating a strong relationship between the strains and the inflammatory process. We found a positive association between an inflammatory process associated with H. pylori of cagA+ strains and chronic gastritis development.
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spelling cagA positive Helicobacter pylori in Brazilian children related to chronic gastritisHelicobacter pylori infectioncagAinflammatory infiltrateHelicobacter pylori is a spiral-shaped Gram-negative bacterium. It colonizes the gastric mucosa of humans and persists for decades if not treated. Helicobacter pylori infection affects more than half of the world's population and invariably results in chronic gastritis. The cagA gene is present in about 60 to 70% of H. pylori strains; it encodes a high-molecular-weight protein (120 to 140 kDa) and several investigators have noted a correlation between strains that possess cagA and the severity of gastric mucosal inflammation. We examined the relation between cagA status in H. pylori strains and chronic gastritis with inflammatory processes in children from Marília, São Paulo, Brazil. One-hundred-twenty-one children were analyzed histopathologically and by polymerase chain reaction (PCR) to detect H. pylori and cagA. We then looked for an association between cagA presence and inflammatory infiltration. Using histology and PCR, we found 47% H. pylori positive infection; 29 children were diagnosed with chronic gastritis, while 28 showed normal mucosa by histopathological analysis. CagA presence was genotyped in both groups, and an inflammatory infiltrate was studied in all infected children with chronic gastritis. We found cagA strains in 20 of 29 (69%) children with chronic gastritis and 18 of 28 (64%) with normal mucosa, demonstrating a strong relationship between the strains and the inflammatory process. We found a positive association between an inflammatory process associated with H. pylori of cagA+ strains and chronic gastritis development.School of Medicine of Marília Department of Genetic and Molecular Biology HemocentroUNIFESP-EPM Department of GeneticFAMEMA Department of PathologyUNIFESP, EPM, Department of GeneticSciELOFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Brazilian Society of Infectious DiseasesSchool of Medicine of Marília Department of Genetic and Molecular Biology HemocentroUniversidade Federal de São Paulo (UNIFESP)FAMEMA Department of PathologyGatti, Luciano Lobo [UNIFESP]Lábio, Roger Willian deSilva, Luiz Carlos daSmith, Marilia de Arruda Cardoso [UNIFESP]Payão, Spencer Luiz Marques [UNIFESP]2015-06-14T13:36:21Z2015-06-14T13:36:21Z2006-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion254-258application/pdfhttp://dx.doi.org/10.1590/S1413-86702006000400008Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 10, n. 4, p. 254-258, 2006.10.1590/S1413-86702006000400008S1413-86702006000400008.pdf1413-8670S1413-86702006000400008http://repositorio.unifesp.br/handle/11600/3174engBrazilian Journal of Infectious Diseasesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-06T05:24:05Zoai:repositorio.unifesp.br/:11600/3174Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-06T05:24:05Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv cagA positive Helicobacter pylori in Brazilian children related to chronic gastritis
title cagA positive Helicobacter pylori in Brazilian children related to chronic gastritis
spellingShingle cagA positive Helicobacter pylori in Brazilian children related to chronic gastritis
Gatti, Luciano Lobo [UNIFESP]
Helicobacter pylori infection
cagA
inflammatory infiltrate
title_short cagA positive Helicobacter pylori in Brazilian children related to chronic gastritis
title_full cagA positive Helicobacter pylori in Brazilian children related to chronic gastritis
title_fullStr cagA positive Helicobacter pylori in Brazilian children related to chronic gastritis
title_full_unstemmed cagA positive Helicobacter pylori in Brazilian children related to chronic gastritis
title_sort cagA positive Helicobacter pylori in Brazilian children related to chronic gastritis
author Gatti, Luciano Lobo [UNIFESP]
author_facet Gatti, Luciano Lobo [UNIFESP]
Lábio, Roger Willian de
Silva, Luiz Carlos da
Smith, Marilia de Arruda Cardoso [UNIFESP]
Payão, Spencer Luiz Marques [UNIFESP]
author_role author
author2 Lábio, Roger Willian de
Silva, Luiz Carlos da
Smith, Marilia de Arruda Cardoso [UNIFESP]
Payão, Spencer Luiz Marques [UNIFESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv School of Medicine of Marília Department of Genetic and Molecular Biology Hemocentro
Universidade Federal de São Paulo (UNIFESP)
FAMEMA Department of Pathology
dc.contributor.author.fl_str_mv Gatti, Luciano Lobo [UNIFESP]
Lábio, Roger Willian de
Silva, Luiz Carlos da
Smith, Marilia de Arruda Cardoso [UNIFESP]
Payão, Spencer Luiz Marques [UNIFESP]
dc.subject.por.fl_str_mv Helicobacter pylori infection
cagA
inflammatory infiltrate
topic Helicobacter pylori infection
cagA
inflammatory infiltrate
description Helicobacter pylori is a spiral-shaped Gram-negative bacterium. It colonizes the gastric mucosa of humans and persists for decades if not treated. Helicobacter pylori infection affects more than half of the world's population and invariably results in chronic gastritis. The cagA gene is present in about 60 to 70% of H. pylori strains; it encodes a high-molecular-weight protein (120 to 140 kDa) and several investigators have noted a correlation between strains that possess cagA and the severity of gastric mucosal inflammation. We examined the relation between cagA status in H. pylori strains and chronic gastritis with inflammatory processes in children from Marília, São Paulo, Brazil. One-hundred-twenty-one children were analyzed histopathologically and by polymerase chain reaction (PCR) to detect H. pylori and cagA. We then looked for an association between cagA presence and inflammatory infiltration. Using histology and PCR, we found 47% H. pylori positive infection; 29 children were diagnosed with chronic gastritis, while 28 showed normal mucosa by histopathological analysis. CagA presence was genotyped in both groups, and an inflammatory infiltrate was studied in all infected children with chronic gastritis. We found cagA strains in 20 of 29 (69%) children with chronic gastritis and 18 of 28 (64%) with normal mucosa, demonstrating a strong relationship between the strains and the inflammatory process. We found a positive association between an inflammatory process associated with H. pylori of cagA+ strains and chronic gastritis development.
publishDate 2006
dc.date.none.fl_str_mv 2006-08-01
2015-06-14T13:36:21Z
2015-06-14T13:36:21Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1413-86702006000400008
Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 10, n. 4, p. 254-258, 2006.
10.1590/S1413-86702006000400008
S1413-86702006000400008.pdf
1413-8670
S1413-86702006000400008
http://repositorio.unifesp.br/handle/11600/3174
url http://dx.doi.org/10.1590/S1413-86702006000400008
http://repositorio.unifesp.br/handle/11600/3174
identifier_str_mv Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 10, n. 4, p. 254-258, 2006.
10.1590/S1413-86702006000400008
S1413-86702006000400008.pdf
1413-8670
S1413-86702006000400008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Infectious Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 254-258
application/pdf
dc.publisher.none.fl_str_mv Brazilian Society of Infectious Diseases
publisher.none.fl_str_mv Brazilian Society of Infectious Diseases
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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