Rapid diagnosis of coccidioidomycosis by nested PCR assay of sputum
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2007 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| dARK ID: | ark:/48912/001300000923f |
| DOI: | 10.1111/j.1469-0691.2006.01679.x |
| Texto Completo: | http://dx.doi.org/10.1111/j.1469-0691.2006.01679.x http://repositorio.unifesp.br/handle/11600/29605 |
Resumo: | Coccidioidomycosis is a deep infection caused by two dimorphic fungi, Coccidioides immitis and Coccidioides posadasii. Diagnosis of the disease requires culture of suspicious clinical samples on mycological media. However, as these species are virulent pathogens, handling of their cultures is a high-risk activity, and is limited to Biosafety Level 3 laboratories. This study describes the direct detection of C. posadasii DNA in an inappropriate sputum sample by PCR amplification of the highly specific Ag2/PRA antigen gene. the results obtained suggest that direct detection of the Ag2/PRA sequence in sputum is an excellent method for rapid and specific diagnosis of coccidioidomycosis. |
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Rapid diagnosis of coccidioidomycosis by nested PCR assay of sputumAg2/PRA genecoccidioidomycosisDNA extractionmolecular diagnosisPCRsputumCoccidioidomycosis is a deep infection caused by two dimorphic fungi, Coccidioides immitis and Coccidioides posadasii. Diagnosis of the disease requires culture of suspicious clinical samples on mycological media. However, as these species are virulent pathogens, handling of their cultures is a high-risk activity, and is limited to Biosafety Level 3 laboratories. This study describes the direct detection of C. posadasii DNA in an inappropriate sputum sample by PCR amplification of the highly specific Ag2/PRA antigen gene. the results obtained suggest that direct detection of the Ag2/PRA sequence in sputum is an excellent method for rapid and specific diagnosis of coccidioidomycosis.Fed Univ Ceara, Med Mycol Specialized Ctr, Fortaleza, Ceara, BrazilFed Univ Ceara, Postgrad Program Med Sci, Fortaleza, Ceara, BrazilState Univ Ceara, Postgrad Program Vet Sci, Fortaleza, Ceara, BrazilState Univ Ceara, Dept Sci Biol, Fortaleza, Ceara, BrazilUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, BrazilWeb of ScienceBlackwell PublishingFed Univ CearaState Univ CearaUniversidade Federal de São Paulo (UNIFESP)Cordeiro, R. de AguiarNogueira Brilhante, R. S.Gadelha Rocha, M. F.Araujo Moura, F. E.Camargo, Zoilo Pires de [UNIFESP]Costa Sidrim, J. J.2016-01-24T12:42:00Z2016-01-24T12:42:00Z2007-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion449-451application/pdfhttp://dx.doi.org/10.1111/j.1469-0691.2006.01679.xClinical Microbiology and Infection. Oxford: Blackwell Publishing, v. 13, n. 4, p. 449-451, 2007.10.1111/j.1469-0691.2006.01679.xWOS000244718200019.pdf1198-743Xhttp://repositorio.unifesp.br/handle/11600/29605WOS:000244718200019ark:/48912/001300000923fengClinical Microbiology and Infectioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-07T15:30:25Zoai:repositorio.unifesp.br/:11600/29605Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T20:06:21.376588Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
Rapid diagnosis of coccidioidomycosis by nested PCR assay of sputum |
| title |
Rapid diagnosis of coccidioidomycosis by nested PCR assay of sputum |
| spellingShingle |
Rapid diagnosis of coccidioidomycosis by nested PCR assay of sputum Rapid diagnosis of coccidioidomycosis by nested PCR assay of sputum Cordeiro, R. de Aguiar Ag2/PRA gene coccidioidomycosis DNA extraction molecular diagnosis PCR sputum Cordeiro, R. de Aguiar Ag2/PRA gene coccidioidomycosis DNA extraction molecular diagnosis PCR sputum |
| title_short |
Rapid diagnosis of coccidioidomycosis by nested PCR assay of sputum |
| title_full |
Rapid diagnosis of coccidioidomycosis by nested PCR assay of sputum |
| title_fullStr |
Rapid diagnosis of coccidioidomycosis by nested PCR assay of sputum Rapid diagnosis of coccidioidomycosis by nested PCR assay of sputum |
| title_full_unstemmed |
Rapid diagnosis of coccidioidomycosis by nested PCR assay of sputum Rapid diagnosis of coccidioidomycosis by nested PCR assay of sputum |
| title_sort |
Rapid diagnosis of coccidioidomycosis by nested PCR assay of sputum |
| author |
Cordeiro, R. de Aguiar |
| author_facet |
Cordeiro, R. de Aguiar Cordeiro, R. de Aguiar Nogueira Brilhante, R. S. Gadelha Rocha, M. F. Araujo Moura, F. E. Camargo, Zoilo Pires de [UNIFESP] Costa Sidrim, J. J. Nogueira Brilhante, R. S. Gadelha Rocha, M. F. Araujo Moura, F. E. Camargo, Zoilo Pires de [UNIFESP] Costa Sidrim, J. J. |
| author_role |
author |
| author2 |
Nogueira Brilhante, R. S. Gadelha Rocha, M. F. Araujo Moura, F. E. Camargo, Zoilo Pires de [UNIFESP] Costa Sidrim, J. J. |
| author2_role |
author author author author author |
| dc.contributor.none.fl_str_mv |
Fed Univ Ceara State Univ Ceara Universidade Federal de São Paulo (UNIFESP) |
| dc.contributor.author.fl_str_mv |
Cordeiro, R. de Aguiar Nogueira Brilhante, R. S. Gadelha Rocha, M. F. Araujo Moura, F. E. Camargo, Zoilo Pires de [UNIFESP] Costa Sidrim, J. J. |
| dc.subject.por.fl_str_mv |
Ag2/PRA gene coccidioidomycosis DNA extraction molecular diagnosis PCR sputum |
| topic |
Ag2/PRA gene coccidioidomycosis DNA extraction molecular diagnosis PCR sputum |
| description |
Coccidioidomycosis is a deep infection caused by two dimorphic fungi, Coccidioides immitis and Coccidioides posadasii. Diagnosis of the disease requires culture of suspicious clinical samples on mycological media. However, as these species are virulent pathogens, handling of their cultures is a high-risk activity, and is limited to Biosafety Level 3 laboratories. This study describes the direct detection of C. posadasii DNA in an inappropriate sputum sample by PCR amplification of the highly specific Ag2/PRA antigen gene. the results obtained suggest that direct detection of the Ag2/PRA sequence in sputum is an excellent method for rapid and specific diagnosis of coccidioidomycosis. |
| publishDate |
2007 |
| dc.date.none.fl_str_mv |
2007-04-01 2016-01-24T12:42:00Z 2016-01-24T12:42:00Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/j.1469-0691.2006.01679.x Clinical Microbiology and Infection. Oxford: Blackwell Publishing, v. 13, n. 4, p. 449-451, 2007. 10.1111/j.1469-0691.2006.01679.x WOS000244718200019.pdf 1198-743X http://repositorio.unifesp.br/handle/11600/29605 WOS:000244718200019 |
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ark:/48912/001300000923f |
| url |
http://dx.doi.org/10.1111/j.1469-0691.2006.01679.x http://repositorio.unifesp.br/handle/11600/29605 |
| identifier_str_mv |
Clinical Microbiology and Infection. Oxford: Blackwell Publishing, v. 13, n. 4, p. 449-451, 2007. 10.1111/j.1469-0691.2006.01679.x WOS000244718200019.pdf 1198-743X WOS:000244718200019 ark:/48912/001300000923f |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Clinical Microbiology and Infection |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
449-451 application/pdf |
| dc.publisher.none.fl_str_mv |
Blackwell Publishing |
| publisher.none.fl_str_mv |
Blackwell Publishing |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
| instname_str |
Universidade Federal de São Paulo (UNIFESP) |
| instacron_str |
UNIFESP |
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UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
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biblioteca.csp@unifesp.br |
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1822183947457527808 |
| dc.identifier.doi.none.fl_str_mv |
10.1111/j.1469-0691.2006.01679.x |