Evaluation of Rhamnetin as an Inhibitor of the Pharmacological Effect of Secretory Phospholipase A2
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| Texto Completo: | http://repositorio.unifesp.br/handle/11600/51284 http://dx.doi.org/10.3390/molecules22091441 |
Resumo: | Rhamnetin (Rhm), 3-O-methylquercetin (3MQ), and Rhamnazin (Rhz) are methylated derivatives of quercetin commonly found in fruits and vegetables that possess antioxidant and anti-inflammatory properties. Phospholipase A2 (PLA2) displays several important roles during acute inflammation |
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Belchor, Mariana Novo [UNIFESP]Gaeta, Henrique HesselBittencourt Rodrigues, Caroline Fabrida Cruz Costa, Caroline RamosToyama, Daniela de OliveiraDomingues Passero, Luiz FelipeLaurenti, Marcia DalastraToyama, Marcos Hikari2019-08-19T11:48:34Z2019-08-19T11:48:34Z2017Molecules. Basel, v. 22, n. 9, p. -, 2017.1420-3049http://repositorio.unifesp.br/handle/11600/51284http://dx.doi.org/10.3390/molecules22091441WOS000411499400046.pdf10.3390/molecules22091441WOS:000411499400046Rhamnetin (Rhm), 3-O-methylquercetin (3MQ), and Rhamnazin (Rhz) are methylated derivatives of quercetin commonly found in fruits and vegetables that possess antioxidant and anti-inflammatory properties. Phospholipase A2 (PLA2) displays several important roles during acute inflammationtherefore, this study aimed at investigating new compounds able to inhibit this enzyme, besides evaluating creatine kinase (CK) levels and citotoxicity. Methylated quercetins were compared with quercetin (Q) and were incubated with secretory PLA2 (sPLA2) from Bothrops jararacussu to determine their inhibitory activity. Cytotoxic studies were performed by using the J774 cell lineage incubated with quercertins. In vivo tests were performed with Swiss female mice to evaluate decreasing paw edema potential and compounds' CK levels. Structural modifications on sPLA2 were made with circular dichroism (CD). Despite Q and Rhz showing greater enzymatic inhibitory potential, high CK was observed. Rhm exhibited sPLA2 inhibitory potential, no toxicity and, remarkably, it decreased CK levels. The presence of 3OH on the C-ring of Rhm may contribute to both its anti-inflammatory and enzymatic inhibition of sPLA2, and the methylation of ring A may provide the increase in cell viability and low CK level induced by sPLA2. These results showed that Rhm can be a candidate as a natural compound for the development of new anti-inflammatory drugs.Universidade Estadual Paulista (UNESP)Universidade Federal de São Paulo (UNIFESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Fed São Paulo, Postgrad Program Food Nutr & Hlth, BR-11015020 São Paulo, BrazilUniv Estadual Paulista, Biosci Inst, BR-11330900 São Paulo, BrazilBrazil Univ, Prorector Res, BR-08230030 São Paulo, BrazilUniv São Paulo, Pathol Lab Infect Dis LIM50, Dept Pathol, Sch Med, BR-01246903 São Paulo, BrazilUniv Fed São Paulo, Postgrad Program Food Nutr & Hlth, BR-11015020 São Paulo, BrazilWeb of Science-engMdpi Agrhamnetinmethylated quercetinsphospholipase A2anti-inflammatoryBothrops jararacussuEvaluation of Rhamnetin as an Inhibitor of the Pharmacological Effect of Secretory Phospholipase A2info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000411499400046.pdfapplication/pdf1205954${dspace.ui.url}/bitstream/11600/51284/1/WOS000411499400046.pdf6e20d32d7739932392ae137a1c426549MD51open accessTEXTWOS000411499400046.pdf.txtWOS000411499400046.pdf.txtExtracted texttext/plain50166${dspace.ui.url}/bitstream/11600/51284/2/WOS000411499400046.pdf.txtce1991e7afffb6ef2cb64bd8c7d2fc78MD52open accessTHUMBNAILWOS000411499400046.pdf.jpgWOS000411499400046.pdf.jpgIM Thumbnailimage/jpeg6789${dspace.ui.url}/bitstream/11600/51284/4/WOS000411499400046.pdf.jpgca574d1617d7c1ad058bb7aa4ce6d8d1MD54open access11600/512842022-08-01 18:43:39.88open accessoai:repositorio.unifesp.br:11600/51284Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-08-01T21:43:39Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.en.fl_str_mv |
Evaluation of Rhamnetin as an Inhibitor of the Pharmacological Effect of Secretory Phospholipase A2 |
| title |
Evaluation of Rhamnetin as an Inhibitor of the Pharmacological Effect of Secretory Phospholipase A2 |
| spellingShingle |
Evaluation of Rhamnetin as an Inhibitor of the Pharmacological Effect of Secretory Phospholipase A2 Belchor, Mariana Novo [UNIFESP] rhamnetin methylated quercetins phospholipase A2 anti-inflammatory Bothrops jararacussu |
| title_short |
Evaluation of Rhamnetin as an Inhibitor of the Pharmacological Effect of Secretory Phospholipase A2 |
| title_full |
Evaluation of Rhamnetin as an Inhibitor of the Pharmacological Effect of Secretory Phospholipase A2 |
| title_fullStr |
Evaluation of Rhamnetin as an Inhibitor of the Pharmacological Effect of Secretory Phospholipase A2 |
| title_full_unstemmed |
Evaluation of Rhamnetin as an Inhibitor of the Pharmacological Effect of Secretory Phospholipase A2 |
| title_sort |
Evaluation of Rhamnetin as an Inhibitor of the Pharmacological Effect of Secretory Phospholipase A2 |
| author |
Belchor, Mariana Novo [UNIFESP] |
| author_facet |
Belchor, Mariana Novo [UNIFESP] Gaeta, Henrique Hessel Bittencourt Rodrigues, Caroline Fabri da Cruz Costa, Caroline Ramos Toyama, Daniela de Oliveira Domingues Passero, Luiz Felipe Laurenti, Marcia Dalastra Toyama, Marcos Hikari |
| author_role |
author |
| author2 |
Gaeta, Henrique Hessel Bittencourt Rodrigues, Caroline Fabri da Cruz Costa, Caroline Ramos Toyama, Daniela de Oliveira Domingues Passero, Luiz Felipe Laurenti, Marcia Dalastra Toyama, Marcos Hikari |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Belchor, Mariana Novo [UNIFESP] Gaeta, Henrique Hessel Bittencourt Rodrigues, Caroline Fabri da Cruz Costa, Caroline Ramos Toyama, Daniela de Oliveira Domingues Passero, Luiz Felipe Laurenti, Marcia Dalastra Toyama, Marcos Hikari |
| dc.subject.eng.fl_str_mv |
rhamnetin methylated quercetins phospholipase A2 anti-inflammatory Bothrops jararacussu |
| topic |
rhamnetin methylated quercetins phospholipase A2 anti-inflammatory Bothrops jararacussu |
| description |
Rhamnetin (Rhm), 3-O-methylquercetin (3MQ), and Rhamnazin (Rhz) are methylated derivatives of quercetin commonly found in fruits and vegetables that possess antioxidant and anti-inflammatory properties. Phospholipase A2 (PLA2) displays several important roles during acute inflammation |
| publishDate |
2017 |
| dc.date.issued.fl_str_mv |
2017 |
| dc.date.accessioned.fl_str_mv |
2019-08-19T11:48:34Z |
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2019-08-19T11:48:34Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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Molecules. Basel, v. 22, n. 9, p. -, 2017. |
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http://repositorio.unifesp.br/handle/11600/51284 http://dx.doi.org/10.3390/molecules22091441 |
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1420-3049 |
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WOS000411499400046.pdf |
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10.3390/molecules22091441 |
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WOS:000411499400046 |
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Molecules. Basel, v. 22, n. 9, p. -, 2017. 1420-3049 WOS000411499400046.pdf 10.3390/molecules22091441 WOS:000411499400046 |
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http://repositorio.unifesp.br/handle/11600/51284 http://dx.doi.org/10.3390/molecules22091441 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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- |
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Mdpi Ag |
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Mdpi Ag |
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