Treadmill Exercise Training Prevents Myocardial Mechanical Dysfunction Induced by Androgenic-Anabolic Steroid Treatment in Rats
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Publication Date: | 2014 |
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Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNIFESP |
Download full: | http://repositorio.unifesp.br/handle/11600/37436 http://dx.doi.org/10.1371/journal.pone.0087106 |
Summary: | Elevated concentrations of testosterone and its synthetic analogs may induce changes in cardiovascular function. However, the effects of the combination of anabolic/androgenic steroid (AAS) treatment and exercise training on systolic and diastolic cardiac function are poorly understood. in the present study, we aimed to investigate the effects of low-dose steroid treatment (stanozolol) on cardiac contractile parameters when this steroid treatment was combined with exercise training in rats and the effects of chronic steroid treatment on the Frank-Starling (length-tension curves) relationship. Male Wistar rats were randomly assigned to one of four groups: U (untrained), US (untrained and treated with stanozolol 5 mg/kg/week), T (trained, 16 m/min/1 h) and TS (trained and treated with stanozolol 5 mg/kg/week). Continuous exercise training was conducted 5 days/week for 8 consecutive weeks. the speed of the treadmill was gradually increased to a final setting of 16 m/min/1 h. Experiments were divided into two independent series: 1) central hemodynamic analysis for mean arterial blood pressure (MAP) and cardiac output (CO) measurements and 2) isolated papillary muscle preparation in Krebs solution. Stanozolol treatment significantly increased the MAP and the heart size in untrained and trained rats (U 113 +/- 2; T 106 +/- 2; US 138 +/- 8 and TS 130 +/- 7 mmHg). Furthermore, stanozolol significantly decreased developed tension and dT/dt (maximal and minimal) in U rats. However, the developed tension was completely restored by training. the Frank/Starling relationship was impaired in rats treated with stanozolol; however, again, training completely restored diastolic function. Taken together, the present data suggest that AAS treatment is able to decrease cardiac performance (systolic and diastolic functions). the combination of stanozolol and physical training improved cardiac performance, including diastolic and systolic functions, independent of changes in central hemodynamic parameters. Therefore, changes in ventricular myocyte calcium transients may play a cardioprotective role. |
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Bocalini, Danilo S.Beutel, Abram [UNIFESP]Bergamaschi, Cassia Toledo [UNIFESP]Tucci, Paulo José Ferreira [UNIFESP]Campos, Ruy Ribeiro [UNIFESP]Univ Sao Judas TadeuUniversidade Federal de São Paulo (UNIFESP)2016-01-24T14:35:18Z2016-01-24T14:35:18Z2014-02-12Plos One. San Francisco: Public Library Science, v. 9, n. 2, 5 p., 2014.1932-6203http://repositorio.unifesp.br/handle/11600/37436http://dx.doi.org/10.1371/journal.pone.0087106WOS000331262600011.pdf10.1371/journal.pone.0087106WOS:000331262600011Elevated concentrations of testosterone and its synthetic analogs may induce changes in cardiovascular function. However, the effects of the combination of anabolic/androgenic steroid (AAS) treatment and exercise training on systolic and diastolic cardiac function are poorly understood. in the present study, we aimed to investigate the effects of low-dose steroid treatment (stanozolol) on cardiac contractile parameters when this steroid treatment was combined with exercise training in rats and the effects of chronic steroid treatment on the Frank-Starling (length-tension curves) relationship. Male Wistar rats were randomly assigned to one of four groups: U (untrained), US (untrained and treated with stanozolol 5 mg/kg/week), T (trained, 16 m/min/1 h) and TS (trained and treated with stanozolol 5 mg/kg/week). Continuous exercise training was conducted 5 days/week for 8 consecutive weeks. the speed of the treadmill was gradually increased to a final setting of 16 m/min/1 h. Experiments were divided into two independent series: 1) central hemodynamic analysis for mean arterial blood pressure (MAP) and cardiac output (CO) measurements and 2) isolated papillary muscle preparation in Krebs solution. Stanozolol treatment significantly increased the MAP and the heart size in untrained and trained rats (U 113 +/- 2; T 106 +/- 2; US 138 +/- 8 and TS 130 +/- 7 mmHg). Furthermore, stanozolol significantly decreased developed tension and dT/dt (maximal and minimal) in U rats. However, the developed tension was completely restored by training. the Frank/Starling relationship was impaired in rats treated with stanozolol; however, again, training completely restored diastolic function. Taken together, the present data suggest that AAS treatment is able to decrease cardiac performance (systolic and diastolic functions). the combination of stanozolol and physical training improved cardiac performance, including diastolic and systolic functions, independent of changes in central hemodynamic parameters. Therefore, changes in ventricular myocyte calcium transients may play a cardioprotective role.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Sao Judas Tadeu, Dept Post Grad Phys Educ, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Physiol, Div Cardiovasc, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Med, Div Cardiol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Physiol, Div Cardiovasc, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Med, Div Cardiol, São Paulo, BrazilWeb of Science5engPublic Library SciencePlos OneTreadmill Exercise Training Prevents Myocardial Mechanical Dysfunction Induced by Androgenic-Anabolic Steroid Treatment in Ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000331262600011.pdfapplication/pdf507402${dspace.ui.url}/bitstream/11600/37436/1/WOS000331262600011.pdf24f8ca2ced2ef8913fbefc5fe8f0630dMD51open accessTEXTWOS000331262600011.pdf.txtWOS000331262600011.pdf.txtExtracted texttext/plain28454${dspace.ui.url}/bitstream/11600/37436/2/WOS000331262600011.pdf.txtb352d56777437a71dc821f9391313104MD52open access11600/374362023-02-15 09:10:17.769open accessoai:repositorio.unifesp.br:11600/37436Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:30:35.313906Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Treadmill Exercise Training Prevents Myocardial Mechanical Dysfunction Induced by Androgenic-Anabolic Steroid Treatment in Rats |
title |
Treadmill Exercise Training Prevents Myocardial Mechanical Dysfunction Induced by Androgenic-Anabolic Steroid Treatment in Rats |
spellingShingle |
Treadmill Exercise Training Prevents Myocardial Mechanical Dysfunction Induced by Androgenic-Anabolic Steroid Treatment in Rats Bocalini, Danilo S. |
title_short |
Treadmill Exercise Training Prevents Myocardial Mechanical Dysfunction Induced by Androgenic-Anabolic Steroid Treatment in Rats |
title_full |
Treadmill Exercise Training Prevents Myocardial Mechanical Dysfunction Induced by Androgenic-Anabolic Steroid Treatment in Rats |
title_fullStr |
Treadmill Exercise Training Prevents Myocardial Mechanical Dysfunction Induced by Androgenic-Anabolic Steroid Treatment in Rats |
title_full_unstemmed |
Treadmill Exercise Training Prevents Myocardial Mechanical Dysfunction Induced by Androgenic-Anabolic Steroid Treatment in Rats |
title_sort |
Treadmill Exercise Training Prevents Myocardial Mechanical Dysfunction Induced by Androgenic-Anabolic Steroid Treatment in Rats |
author |
Bocalini, Danilo S. |
author_facet |
Bocalini, Danilo S. Beutel, Abram [UNIFESP] Bergamaschi, Cassia Toledo [UNIFESP] Tucci, Paulo José Ferreira [UNIFESP] Campos, Ruy Ribeiro [UNIFESP] |
author_role |
author |
author2 |
Beutel, Abram [UNIFESP] Bergamaschi, Cassia Toledo [UNIFESP] Tucci, Paulo José Ferreira [UNIFESP] Campos, Ruy Ribeiro [UNIFESP] |
author2_role |
author author author author |
dc.contributor.institution.none.fl_str_mv |
Univ Sao Judas Tadeu Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Bocalini, Danilo S. Beutel, Abram [UNIFESP] Bergamaschi, Cassia Toledo [UNIFESP] Tucci, Paulo José Ferreira [UNIFESP] Campos, Ruy Ribeiro [UNIFESP] |
description |
Elevated concentrations of testosterone and its synthetic analogs may induce changes in cardiovascular function. However, the effects of the combination of anabolic/androgenic steroid (AAS) treatment and exercise training on systolic and diastolic cardiac function are poorly understood. in the present study, we aimed to investigate the effects of low-dose steroid treatment (stanozolol) on cardiac contractile parameters when this steroid treatment was combined with exercise training in rats and the effects of chronic steroid treatment on the Frank-Starling (length-tension curves) relationship. Male Wistar rats were randomly assigned to one of four groups: U (untrained), US (untrained and treated with stanozolol 5 mg/kg/week), T (trained, 16 m/min/1 h) and TS (trained and treated with stanozolol 5 mg/kg/week). Continuous exercise training was conducted 5 days/week for 8 consecutive weeks. the speed of the treadmill was gradually increased to a final setting of 16 m/min/1 h. Experiments were divided into two independent series: 1) central hemodynamic analysis for mean arterial blood pressure (MAP) and cardiac output (CO) measurements and 2) isolated papillary muscle preparation in Krebs solution. Stanozolol treatment significantly increased the MAP and the heart size in untrained and trained rats (U 113 +/- 2; T 106 +/- 2; US 138 +/- 8 and TS 130 +/- 7 mmHg). Furthermore, stanozolol significantly decreased developed tension and dT/dt (maximal and minimal) in U rats. However, the developed tension was completely restored by training. the Frank/Starling relationship was impaired in rats treated with stanozolol; however, again, training completely restored diastolic function. Taken together, the present data suggest that AAS treatment is able to decrease cardiac performance (systolic and diastolic functions). the combination of stanozolol and physical training improved cardiac performance, including diastolic and systolic functions, independent of changes in central hemodynamic parameters. Therefore, changes in ventricular myocyte calcium transients may play a cardioprotective role. |
publishDate |
2014 |
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2014-02-12 |
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2016-01-24T14:35:18Z |
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2016-01-24T14:35:18Z |
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Plos One. San Francisco: Public Library Science, v. 9, n. 2, 5 p., 2014. |
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http://repositorio.unifesp.br/handle/11600/37436 http://dx.doi.org/10.1371/journal.pone.0087106 |
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1932-6203 |
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Plos One. San Francisco: Public Library Science, v. 9, n. 2, 5 p., 2014. 1932-6203 WOS000331262600011.pdf 10.1371/journal.pone.0087106 WOS:000331262600011 |
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