Experimental model of cultured keratinocytes

Detalhes bibliográficos
Autor(a) principal: Gragnani, Alfredo [UNIFESP]
Data de Publicação: 2003
Outros Autores: Morgan, Jeffrey R., Ferreira, Lydia Masako [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
dARK ID: ark:/48912/00130000174j3
Texto Completo: http://dx.doi.org/10.1590/S0102-86502003001100002
http://repositorio.unifesp.br/handle/11600/1610
Resumo: The bioengineering research is essential in the development of ideal combination of biomaterials and cultured cells to produce the permanent wound coverage. The experimental model of cultured keratinocytes presents all steps of the culture, since the isolation of the keratinocytes, preparation of the human acellular dermis, preparation of the composite skin graft and their elevation to the air-liquid interface. The research in cultured keratinocytes model advances in two main ways: 1. optimization of the methods in vitro to the skin cells culture and proliferation and 2. developing biomaterials that present similar skin properties.
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spelling Experimental model of cultured keratinocytesModelo experimental de cultura de queratinócitosKeratinocyteHuman acellular dermisSkin transplantationFibroblastsCultured cellQueratinócitosDerme acelular humanaTransplante de peleFibroblastosCultura de célulasThe bioengineering research is essential in the development of ideal combination of biomaterials and cultured cells to produce the permanent wound coverage. The experimental model of cultured keratinocytes presents all steps of the culture, since the isolation of the keratinocytes, preparation of the human acellular dermis, preparation of the composite skin graft and their elevation to the air-liquid interface. The research in cultured keratinocytes model advances in two main ways: 1. optimization of the methods in vitro to the skin cells culture and proliferation and 2. developing biomaterials that present similar skin properties.A pesquisa em bioengenharia é primordial no desenvolvimento da combinação ideal de biomateriais e células cultivadas para produzir a cobertura definitiva das lesões. O modelo experimental da cultura de queratinócitos apresenta toda as etapas do cultivo, desde o isolamento dos queratinócitos, preparação da derme acelular humana, do enxerto composto e da sua elevação à interface ar-líquido. A pesquisa em modelo de cultura de queratinócitos desenvolve-se em duas vias principais: 1. otimização dos métodos in vitro para cultivo e proliferação de células da pele e 2. desenvolvimento de biomateriais que mimetizem as propriedades da pele.UNIFESP-EPMShriners Hospital for Children Harvard Medical SchoolUNIFESP-EPM Surgery DepartmentUNIFESP, EPM, Surgery DepartmentSciELOSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaUniversidade Federal de São Paulo (UNIFESP)Shriners Hospital for Children Harvard Medical SchoolGragnani, Alfredo [UNIFESP]Morgan, Jeffrey R.Ferreira, Lydia Masako [UNIFESP]2015-06-14T13:29:53Z2015-06-14T13:29:53Z2003-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion04-14application/pdfhttp://dx.doi.org/10.1590/S0102-86502003001100002Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 18, n. spe, p. 04-14, 2003.10.1590/S0102-86502003001100002S0102-86502003001100002.pdf0102-8650S0102-86502003001100002http://repositorio.unifesp.br/handle/11600/1610ark:/48912/00130000174j3engActa Cirurgica Brasileirainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-04T05:40:39Zoai:repositorio.unifesp.br/:11600/1610Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T21:01:05.772067Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Experimental model of cultured keratinocytes
Modelo experimental de cultura de queratinócitos
title Experimental model of cultured keratinocytes
spellingShingle Experimental model of cultured keratinocytes
Gragnani, Alfredo [UNIFESP]
Keratinocyte
Human acellular dermis
Skin transplantation
Fibroblasts
Cultured cell
Queratinócitos
Derme acelular humana
Transplante de pele
Fibroblastos
Cultura de células
title_short Experimental model of cultured keratinocytes
title_full Experimental model of cultured keratinocytes
title_fullStr Experimental model of cultured keratinocytes
title_full_unstemmed Experimental model of cultured keratinocytes
title_sort Experimental model of cultured keratinocytes
author Gragnani, Alfredo [UNIFESP]
author_facet Gragnani, Alfredo [UNIFESP]
Morgan, Jeffrey R.
Ferreira, Lydia Masako [UNIFESP]
author_role author
author2 Morgan, Jeffrey R.
Ferreira, Lydia Masako [UNIFESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Shriners Hospital for Children Harvard Medical School
dc.contributor.author.fl_str_mv Gragnani, Alfredo [UNIFESP]
Morgan, Jeffrey R.
Ferreira, Lydia Masako [UNIFESP]
dc.subject.por.fl_str_mv Keratinocyte
Human acellular dermis
Skin transplantation
Fibroblasts
Cultured cell
Queratinócitos
Derme acelular humana
Transplante de pele
Fibroblastos
Cultura de células
topic Keratinocyte
Human acellular dermis
Skin transplantation
Fibroblasts
Cultured cell
Queratinócitos
Derme acelular humana
Transplante de pele
Fibroblastos
Cultura de células
description The bioengineering research is essential in the development of ideal combination of biomaterials and cultured cells to produce the permanent wound coverage. The experimental model of cultured keratinocytes presents all steps of the culture, since the isolation of the keratinocytes, preparation of the human acellular dermis, preparation of the composite skin graft and their elevation to the air-liquid interface. The research in cultured keratinocytes model advances in two main ways: 1. optimization of the methods in vitro to the skin cells culture and proliferation and 2. developing biomaterials that present similar skin properties.
publishDate 2003
dc.date.none.fl_str_mv 2003-01-01
2015-06-14T13:29:53Z
2015-06-14T13:29:53Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0102-86502003001100002
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 18, n. spe, p. 04-14, 2003.
10.1590/S0102-86502003001100002
S0102-86502003001100002.pdf
0102-8650
S0102-86502003001100002
http://repositorio.unifesp.br/handle/11600/1610
dc.identifier.dark.fl_str_mv ark:/48912/00130000174j3
url http://dx.doi.org/10.1590/S0102-86502003001100002
http://repositorio.unifesp.br/handle/11600/1610
identifier_str_mv Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 18, n. spe, p. 04-14, 2003.
10.1590/S0102-86502003001100002
S0102-86502003001100002.pdf
0102-8650
S0102-86502003001100002
ark:/48912/00130000174j3
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Cirurgica Brasileira
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 04-14
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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