Experimental model of cultured keratinocytes
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
dARK ID: | ark:/48912/00130000174j3 |
Texto Completo: | http://dx.doi.org/10.1590/S0102-86502003001100002 http://repositorio.unifesp.br/handle/11600/1610 |
Resumo: | The bioengineering research is essential in the development of ideal combination of biomaterials and cultured cells to produce the permanent wound coverage. The experimental model of cultured keratinocytes presents all steps of the culture, since the isolation of the keratinocytes, preparation of the human acellular dermis, preparation of the composite skin graft and their elevation to the air-liquid interface. The research in cultured keratinocytes model advances in two main ways: 1. optimization of the methods in vitro to the skin cells culture and proliferation and 2. developing biomaterials that present similar skin properties. |
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Experimental model of cultured keratinocytesModelo experimental de cultura de queratinócitosKeratinocyteHuman acellular dermisSkin transplantationFibroblastsCultured cellQueratinócitosDerme acelular humanaTransplante de peleFibroblastosCultura de célulasThe bioengineering research is essential in the development of ideal combination of biomaterials and cultured cells to produce the permanent wound coverage. The experimental model of cultured keratinocytes presents all steps of the culture, since the isolation of the keratinocytes, preparation of the human acellular dermis, preparation of the composite skin graft and their elevation to the air-liquid interface. The research in cultured keratinocytes model advances in two main ways: 1. optimization of the methods in vitro to the skin cells culture and proliferation and 2. developing biomaterials that present similar skin properties.A pesquisa em bioengenharia é primordial no desenvolvimento da combinação ideal de biomateriais e células cultivadas para produzir a cobertura definitiva das lesões. O modelo experimental da cultura de queratinócitos apresenta toda as etapas do cultivo, desde o isolamento dos queratinócitos, preparação da derme acelular humana, do enxerto composto e da sua elevação à interface ar-líquido. A pesquisa em modelo de cultura de queratinócitos desenvolve-se em duas vias principais: 1. otimização dos métodos in vitro para cultivo e proliferação de células da pele e 2. desenvolvimento de biomateriais que mimetizem as propriedades da pele.UNIFESP-EPMShriners Hospital for Children Harvard Medical SchoolUNIFESP-EPM Surgery DepartmentUNIFESP, EPM, Surgery DepartmentSciELOSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaUniversidade Federal de São Paulo (UNIFESP)Shriners Hospital for Children Harvard Medical SchoolGragnani, Alfredo [UNIFESP]Morgan, Jeffrey R.Ferreira, Lydia Masako [UNIFESP]2015-06-14T13:29:53Z2015-06-14T13:29:53Z2003-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion04-14application/pdfhttp://dx.doi.org/10.1590/S0102-86502003001100002Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 18, n. spe, p. 04-14, 2003.10.1590/S0102-86502003001100002S0102-86502003001100002.pdf0102-8650S0102-86502003001100002http://repositorio.unifesp.br/handle/11600/1610ark:/48912/00130000174j3engActa Cirurgica Brasileirainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-04T05:40:39Zoai:repositorio.unifesp.br/:11600/1610Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T21:01:05.772067Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Experimental model of cultured keratinocytes Modelo experimental de cultura de queratinócitos |
title |
Experimental model of cultured keratinocytes |
spellingShingle |
Experimental model of cultured keratinocytes Gragnani, Alfredo [UNIFESP] Keratinocyte Human acellular dermis Skin transplantation Fibroblasts Cultured cell Queratinócitos Derme acelular humana Transplante de pele Fibroblastos Cultura de células |
title_short |
Experimental model of cultured keratinocytes |
title_full |
Experimental model of cultured keratinocytes |
title_fullStr |
Experimental model of cultured keratinocytes |
title_full_unstemmed |
Experimental model of cultured keratinocytes |
title_sort |
Experimental model of cultured keratinocytes |
author |
Gragnani, Alfredo [UNIFESP] |
author_facet |
Gragnani, Alfredo [UNIFESP] Morgan, Jeffrey R. Ferreira, Lydia Masako [UNIFESP] |
author_role |
author |
author2 |
Morgan, Jeffrey R. Ferreira, Lydia Masako [UNIFESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Shriners Hospital for Children Harvard Medical School |
dc.contributor.author.fl_str_mv |
Gragnani, Alfredo [UNIFESP] Morgan, Jeffrey R. Ferreira, Lydia Masako [UNIFESP] |
dc.subject.por.fl_str_mv |
Keratinocyte Human acellular dermis Skin transplantation Fibroblasts Cultured cell Queratinócitos Derme acelular humana Transplante de pele Fibroblastos Cultura de células |
topic |
Keratinocyte Human acellular dermis Skin transplantation Fibroblasts Cultured cell Queratinócitos Derme acelular humana Transplante de pele Fibroblastos Cultura de células |
description |
The bioengineering research is essential in the development of ideal combination of biomaterials and cultured cells to produce the permanent wound coverage. The experimental model of cultured keratinocytes presents all steps of the culture, since the isolation of the keratinocytes, preparation of the human acellular dermis, preparation of the composite skin graft and their elevation to the air-liquid interface. The research in cultured keratinocytes model advances in two main ways: 1. optimization of the methods in vitro to the skin cells culture and proliferation and 2. developing biomaterials that present similar skin properties. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-01-01 2015-06-14T13:29:53Z 2015-06-14T13:29:53Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0102-86502003001100002 Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 18, n. spe, p. 04-14, 2003. 10.1590/S0102-86502003001100002 S0102-86502003001100002.pdf 0102-8650 S0102-86502003001100002 http://repositorio.unifesp.br/handle/11600/1610 |
dc.identifier.dark.fl_str_mv |
ark:/48912/00130000174j3 |
url |
http://dx.doi.org/10.1590/S0102-86502003001100002 http://repositorio.unifesp.br/handle/11600/1610 |
identifier_str_mv |
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 18, n. spe, p. 04-14, 2003. 10.1590/S0102-86502003001100002 S0102-86502003001100002.pdf 0102-8650 S0102-86502003001100002 ark:/48912/00130000174j3 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Cirurgica Brasileira |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
04-14 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1818602586381484032 |