Antioxidant, anti-acetylcholinesterase and cytotoxic activities of ethanol extracts of peel, pulp and seeds of exotic Brazilian fruits Antioxidant, anti-acetylcholinesterase and cytotoxic activities in fruits

Detalhes bibliográficos
Autor(a) principal: Omena, Cristhiane Maria Bazilio
Data de Publicação: 2012
Outros Autores: Valentim, Iara Barros, Guedes, Glaucevane da Silva, Rabelo, Luiza Antas, Mano, Camila Marinho, Etelvino, José Henriques Bechara, [UNIFESP], Sawaya, Alexandra Christine Helena Frankland, Trevisan, Maria Teresa Salles, Costa, Joao Gomes da, Ferreira, Roberta Costa Silva, Sant'Ana, Antonio Euzebio Goulart, Goulart, Marilia Oliveira Fonseca
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1016/j.foodres.2012.07.010
http://repositorio.unifesp.br/handle/11600/35420
Resumo: Ethanol extracts of powdered genipap (Genipa americana L), umbu (Spondia tuberosa A.) and siriguela (Spondia purpurea L) prepared from separate pulp, seeds and peel were investigated for their (i) antioxidant capacity, which was evaluated by various known methods; (ii) acetylcholinesterase (AChE) inhibitory activity; and (iii) cytotoxic effect on corneal epithelial cells of sheep. the highest values of total phenolic content were obtained with peel and seed extracts. Siriguela and umbu (seeds and peel) extracts displayed the highest antioxidant activities. Lipid peroxidation assays using mimetic biomembranes and mouse liver homogenates indicated that genipap pulp is a promising antioxidant. the investigation of phenols and organic acid contents revealed the presence of quercetin, citric and quinic acids, chlorogenic acid derivatives, among others, in several extracts, with the highest amount found in siriguela seeds. Genipap pulp and siriguela seed ethanol extracts presented an AChE inhibition zone similar to that of the positive control, carbachol. AChE inhibition assay with chlorogenic acid, one of the main constituents of siriguela seeds, revealed that this acid showed activity similar to that of the control physostigmine. These data suggest that these extracts are potentially important antioxidant supplements for the everyday human diet, pharmaceutical and cosmetic industries. (C) 2012 Elsevier B.V. All rights reserved.
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spelling Antioxidant, anti-acetylcholinesterase and cytotoxic activities of ethanol extracts of peel, pulp and seeds of exotic Brazilian fruits Antioxidant, anti-acetylcholinesterase and cytotoxic activities in fruitsTotal phenolic contentFree radical scavenging activityAntioxidant capacityBODIPYAcetylcholinesterase inhibitionTropical fruitsEthanol extracts of powdered genipap (Genipa americana L), umbu (Spondia tuberosa A.) and siriguela (Spondia purpurea L) prepared from separate pulp, seeds and peel were investigated for their (i) antioxidant capacity, which was evaluated by various known methods; (ii) acetylcholinesterase (AChE) inhibitory activity; and (iii) cytotoxic effect on corneal epithelial cells of sheep. the highest values of total phenolic content were obtained with peel and seed extracts. Siriguela and umbu (seeds and peel) extracts displayed the highest antioxidant activities. Lipid peroxidation assays using mimetic biomembranes and mouse liver homogenates indicated that genipap pulp is a promising antioxidant. the investigation of phenols and organic acid contents revealed the presence of quercetin, citric and quinic acids, chlorogenic acid derivatives, among others, in several extracts, with the highest amount found in siriguela seeds. Genipap pulp and siriguela seed ethanol extracts presented an AChE inhibition zone similar to that of the positive control, carbachol. AChE inhibition assay with chlorogenic acid, one of the main constituents of siriguela seeds, revealed that this acid showed activity similar to that of the control physostigmine. These data suggest that these extracts are potentially important antioxidant supplements for the everyday human diet, pharmaceutical and cosmetic industries. (C) 2012 Elsevier B.V. All rights reserved.Univ Fed Alagoas, Inst Quim & Biotecnol, BR-57072970 Maceio, AL, BrazilUniv Pernambuco, BR-56300000 Petrolina, PE, BrazilUniv Fed Alagoas, Rede Nordeste Biotecnol RENORBIO, BR-57072970 Maceio, AL, BrazilUniv Fed Alagoas, Inst Ciencias Biol & Saude, BR-57072970 Maceio, AL, BrazilUniv São Paulo, Inst Quim, Dept Bioquim, BR-05508000 São Paulo, BrazilUniversidade Federal de São Paulo, Inst Ciencias Ambientais Quim & Farmaceut, BR-09972270 Diadema, SP, BrazilUniv Estadual Campinas, Inst Biol, Dept Biol Vegetal, Campinas, SP, BrazilUniv Fed Ceara, Dept Quim Organ & Inorgan, BR-60021970 Fortaleza, CE, BrazilEmbrapa Tabuleiros Costeiros, Tabuleiro Martins, BR-57061970 Maceio, AL, BrazilUniversidade Federal de São Paulo, Inst Ciencias Ambientais Quim & Farmaceut, BR-09972270 Diadema, SP, BrazilWeb of ScienceConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)INCT-RedoxomaINCT-BioanaliticaFAPESP: 2008/58035-6Elsevier B.V.Univ Fed AlagoasUniv PernambucoUniversidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)Universidade Estadual de Campinas (UNICAMP)Univ Fed CearaEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Omena, Cristhiane Maria BazilioValentim, Iara BarrosGuedes, Glaucevane da SilvaRabelo, Luiza AntasMano, Camila MarinhoEtelvino, José Henriques Bechara, [UNIFESP]Sawaya, Alexandra Christine Helena FranklandTrevisan, Maria Teresa SallesCosta, Joao Gomes daFerreira, Roberta Costa SilvaSant'Ana, Antonio Euzebio GoulartGoulart, Marilia Oliveira Fonseca2016-01-24T14:27:54Z2016-01-24T14:27:54Z2012-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion334-344application/pdfhttp://dx.doi.org/10.1016/j.foodres.2012.07.010Food Research International. Amsterdam: Elsevier B.V., v. 49, n. 1, p. 334-344, 2012.10.1016/j.foodres.2012.07.010WOS000311592500043.pdf0963-9969http://repositorio.unifesp.br/handle/11600/35420WOS:000311592500043engFood Research Internationalinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-08T15:34:43Zoai:repositorio.unifesp.br/:11600/35420Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-08T15:34:43Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Antioxidant, anti-acetylcholinesterase and cytotoxic activities of ethanol extracts of peel, pulp and seeds of exotic Brazilian fruits Antioxidant, anti-acetylcholinesterase and cytotoxic activities in fruits
title Antioxidant, anti-acetylcholinesterase and cytotoxic activities of ethanol extracts of peel, pulp and seeds of exotic Brazilian fruits Antioxidant, anti-acetylcholinesterase and cytotoxic activities in fruits
spellingShingle Antioxidant, anti-acetylcholinesterase and cytotoxic activities of ethanol extracts of peel, pulp and seeds of exotic Brazilian fruits Antioxidant, anti-acetylcholinesterase and cytotoxic activities in fruits
Omena, Cristhiane Maria Bazilio
Total phenolic content
Free radical scavenging activity
Antioxidant capacity
BODIPY
Acetylcholinesterase inhibition
Tropical fruits
title_short Antioxidant, anti-acetylcholinesterase and cytotoxic activities of ethanol extracts of peel, pulp and seeds of exotic Brazilian fruits Antioxidant, anti-acetylcholinesterase and cytotoxic activities in fruits
title_full Antioxidant, anti-acetylcholinesterase and cytotoxic activities of ethanol extracts of peel, pulp and seeds of exotic Brazilian fruits Antioxidant, anti-acetylcholinesterase and cytotoxic activities in fruits
title_fullStr Antioxidant, anti-acetylcholinesterase and cytotoxic activities of ethanol extracts of peel, pulp and seeds of exotic Brazilian fruits Antioxidant, anti-acetylcholinesterase and cytotoxic activities in fruits
title_full_unstemmed Antioxidant, anti-acetylcholinesterase and cytotoxic activities of ethanol extracts of peel, pulp and seeds of exotic Brazilian fruits Antioxidant, anti-acetylcholinesterase and cytotoxic activities in fruits
title_sort Antioxidant, anti-acetylcholinesterase and cytotoxic activities of ethanol extracts of peel, pulp and seeds of exotic Brazilian fruits Antioxidant, anti-acetylcholinesterase and cytotoxic activities in fruits
author Omena, Cristhiane Maria Bazilio
author_facet Omena, Cristhiane Maria Bazilio
Valentim, Iara Barros
Guedes, Glaucevane da Silva
Rabelo, Luiza Antas
Mano, Camila Marinho
Etelvino, José Henriques Bechara, [UNIFESP]
Sawaya, Alexandra Christine Helena Frankland
Trevisan, Maria Teresa Salles
Costa, Joao Gomes da
Ferreira, Roberta Costa Silva
Sant'Ana, Antonio Euzebio Goulart
Goulart, Marilia Oliveira Fonseca
author_role author
author2 Valentim, Iara Barros
Guedes, Glaucevane da Silva
Rabelo, Luiza Antas
Mano, Camila Marinho
Etelvino, José Henriques Bechara, [UNIFESP]
Sawaya, Alexandra Christine Helena Frankland
Trevisan, Maria Teresa Salles
Costa, Joao Gomes da
Ferreira, Roberta Costa Silva
Sant'Ana, Antonio Euzebio Goulart
Goulart, Marilia Oliveira Fonseca
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Univ Fed Alagoas
Univ Pernambuco
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
Univ Fed Ceara
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.author.fl_str_mv Omena, Cristhiane Maria Bazilio
Valentim, Iara Barros
Guedes, Glaucevane da Silva
Rabelo, Luiza Antas
Mano, Camila Marinho
Etelvino, José Henriques Bechara, [UNIFESP]
Sawaya, Alexandra Christine Helena Frankland
Trevisan, Maria Teresa Salles
Costa, Joao Gomes da
Ferreira, Roberta Costa Silva
Sant'Ana, Antonio Euzebio Goulart
Goulart, Marilia Oliveira Fonseca
dc.subject.por.fl_str_mv Total phenolic content
Free radical scavenging activity
Antioxidant capacity
BODIPY
Acetylcholinesterase inhibition
Tropical fruits
topic Total phenolic content
Free radical scavenging activity
Antioxidant capacity
BODIPY
Acetylcholinesterase inhibition
Tropical fruits
description Ethanol extracts of powdered genipap (Genipa americana L), umbu (Spondia tuberosa A.) and siriguela (Spondia purpurea L) prepared from separate pulp, seeds and peel were investigated for their (i) antioxidant capacity, which was evaluated by various known methods; (ii) acetylcholinesterase (AChE) inhibitory activity; and (iii) cytotoxic effect on corneal epithelial cells of sheep. the highest values of total phenolic content were obtained with peel and seed extracts. Siriguela and umbu (seeds and peel) extracts displayed the highest antioxidant activities. Lipid peroxidation assays using mimetic biomembranes and mouse liver homogenates indicated that genipap pulp is a promising antioxidant. the investigation of phenols and organic acid contents revealed the presence of quercetin, citric and quinic acids, chlorogenic acid derivatives, among others, in several extracts, with the highest amount found in siriguela seeds. Genipap pulp and siriguela seed ethanol extracts presented an AChE inhibition zone similar to that of the positive control, carbachol. AChE inhibition assay with chlorogenic acid, one of the main constituents of siriguela seeds, revealed that this acid showed activity similar to that of the control physostigmine. These data suggest that these extracts are potentially important antioxidant supplements for the everyday human diet, pharmaceutical and cosmetic industries. (C) 2012 Elsevier B.V. All rights reserved.
publishDate 2012
dc.date.none.fl_str_mv 2012-11-01
2016-01-24T14:27:54Z
2016-01-24T14:27:54Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.foodres.2012.07.010
Food Research International. Amsterdam: Elsevier B.V., v. 49, n. 1, p. 334-344, 2012.
10.1016/j.foodres.2012.07.010
WOS000311592500043.pdf
0963-9969
http://repositorio.unifesp.br/handle/11600/35420
WOS:000311592500043
url http://dx.doi.org/10.1016/j.foodres.2012.07.010
http://repositorio.unifesp.br/handle/11600/35420
identifier_str_mv Food Research International. Amsterdam: Elsevier B.V., v. 49, n. 1, p. 334-344, 2012.
10.1016/j.foodres.2012.07.010
WOS000311592500043.pdf
0963-9969
WOS:000311592500043
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Food Research International
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.format.none.fl_str_mv 334-344
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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