Efeito da melatonina, metformina e citrato de clomifeno no ovário de ratas em estro permanente

• Autor(a) principal: Lombardi, Leonardo Augusto [UNIFESP]
• Data de Publicação: 2016
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=4017000; http://repositorio.unifesp.br/handle/11600/47489
• topic: Ovary; Polycystic ovarian syndrome; Melatonin; Metformin; Clomiphene citrate; Ovário; Síndrome do ovário policístico; Melatonina; Metformina; Citrato de clomifeno

Analysing histomorphometric and immunohistochemical changes in the interstitial cells and ovarian follicles of rats treated with melatonin, metformin and clomiphene citrate before and after induction of permanent estrus. Methods: Seventy rats were divided into thirteen groups. The groups were divided into 2 experiments. Experiment 1 was composed of rats in the physiological estrus phase (GSham), rats in continuous light induced permanent estrus (GCtrl). In addition, a second group of rats received melatonin, metformin or clomiphene citrate in isolation and associated for a period of 60 days of exposure to continuous illumination, being considered as a prevention group. Experiment 2 was composed of animals in permanent estrus previously induced by the 60 day exposure by continuous illumination that after exposure to light, remained exposed and received melatonin, metformin and Clomiphene citrate as a form of treatment. GSham animals remained in the light period from 7:00 am to 7:00 p.m., while animals from the control group and other treated groups remained under continuous illumination (400 Lux) for a period of 60 days for experiment 1 and a total of 120 days for experiment 2. At the end of this period all animals were anesthetized. Futhermore, the ovaries were removed, fixed in 10% formalin and processed for inclusion in paraffin. Histological sections with 5 ?m were collected on slides and submitted to HE staining for morphological analysis and immunohistochemical methods for the detection of anti-Ki-67 (cell proliferation) and anti-caspase-3 (Apoptosis). Results: The presence of luteal bodies and an increase in the number of primary and antral follicles were observed in all animals treated in both experiments, as well as a decrease in the area occupied by interstitial cells and in the nuclear volume of interstitial cells. These data is more evident in animals treated with melatonin alone and associated with another drug. The immunohistochemical findings presented greater expression of follicular cells to ki-67 in all animals treated in both experiments, being these data more expressive in animals treated with clomiifene citrate. The immunoexpression to caspase 3 was shown to be significantly greater in interstitial cells and lower in granulosa cells in treated animals from both experiments. Conclusion: The administration of melatonin, metformin and clomiphene citrate applied alone or in combination, before and after induction to the permanent estrous state showed an improvement in physiological function and in the ovarian morphology.