Detection of group A beta-hemolytic Streptococcus employing three different detection methods: culture, rapid antigen detecting test, and molecular assay
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/1885 http://dx.doi.org/10.1590/S1413-86702003000500003 |
Resumo: | In order to study the prevalence of Group A beta-hemolytic Streptococcus (GABHS) pharyngotonsillitis in our pediatric population and to compare different sampling methods of GABHS detection, oropharyngeal swabs from 50 children with acute pharyngotonsillitis, between 1 and 12 years old, were used simultaneously for culture, molecular assay and rapid GABHS antigen detection tests. All children were clinically examined at the Division of Pediatric Otorhinolaryngology of the Federal University of São Paulo. Diagnostic criteria were based on signs and symptoms, including sore throat, fever and oropharyngeal purulent secretion. Children that had been treated with antibiotics were excluded. Overall, combining the three methods, the prevalence of GABHS was 34%. GABHS was diagnosed in 30% of the bacterial cultures, in 25% of the samples tested with the molecular nucleic acid hybridization method and in 26% of the cases tested with the rapid antigen detection test. There was no significant difference between these three methods. |
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Santos, Odimara [UNIFESP]Weckx, Luc Louis Maurice [UNIFESP]Pignatari, Antonio Carlos Campos [UNIFESP]Pignatari, Shirley Shizue Nagata [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2015-06-14T13:30:10Z2015-06-14T13:30:10Z2003-10-01Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 7, n. 5, p. 297-300, 2003.1413-8670http://repositorio.unifesp.br/handle/11600/1885http://dx.doi.org/10.1590/S1413-86702003000500003S1413-86702003000500003.pdfS1413-8670200300050000310.1590/S1413-86702003000500003In order to study the prevalence of Group A beta-hemolytic Streptococcus (GABHS) pharyngotonsillitis in our pediatric population and to compare different sampling methods of GABHS detection, oropharyngeal swabs from 50 children with acute pharyngotonsillitis, between 1 and 12 years old, were used simultaneously for culture, molecular assay and rapid GABHS antigen detection tests. All children were clinically examined at the Division of Pediatric Otorhinolaryngology of the Federal University of São Paulo. Diagnostic criteria were based on signs and symptoms, including sore throat, fever and oropharyngeal purulent secretion. Children that had been treated with antibiotics were excluded. Overall, combining the three methods, the prevalence of GABHS was 34%. GABHS was diagnosed in 30% of the bacterial cultures, in 25% of the samples tested with the molecular nucleic acid hybridization method and in 26% of the cases tested with the rapid antigen detection test. There was no significant difference between these three methods.Federal University of São Paulo Division of Pediatric OtolaryngologyUNIFESP, Division of Pediatric OtolaryngologySciELO297-300engBrazilian Society of Infectious DiseasesBrazilian Journal of Infectious DiseasesAcute pharyngotonsillitisGABHSlaboratory detectionDetection of group A beta-hemolytic Streptococcus employing three different detection methods: culture, rapid antigen detecting test, and molecular assayinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS1413-86702003000500003.pdfapplication/pdf20111${dspace.ui.url}/bitstream/11600/1885/1/S1413-86702003000500003.pdf42e4aff0f41d8641b7328d2c801d7b2aMD51open accessTEXTS1413-86702003000500003.pdf.txtS1413-86702003000500003.pdf.txtExtracted texttext/plain14669${dspace.ui.url}/bitstream/11600/1885/21/S1413-86702003000500003.pdf.txt09717c94d921156757ebe007415dbf13MD521open accessTHUMBNAILS1413-86702003000500003.pdf.jpgS1413-86702003000500003.pdf.jpgIM Thumbnailimage/jpeg6388${dspace.ui.url}/bitstream/11600/1885/23/S1413-86702003000500003.pdf.jpg9baee780f97eb5019d51611d91054c69MD523open access11600/18852023-06-05 19:29:47.188open accessoai:repositorio.unifesp.br:11600/1885Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:29:47Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Detection of group A beta-hemolytic Streptococcus employing three different detection methods: culture, rapid antigen detecting test, and molecular assay |
title |
Detection of group A beta-hemolytic Streptococcus employing three different detection methods: culture, rapid antigen detecting test, and molecular assay |
spellingShingle |
Detection of group A beta-hemolytic Streptococcus employing three different detection methods: culture, rapid antigen detecting test, and molecular assay Santos, Odimara [UNIFESP] Acute pharyngotonsillitis GABHS laboratory detection |
title_short |
Detection of group A beta-hemolytic Streptococcus employing three different detection methods: culture, rapid antigen detecting test, and molecular assay |
title_full |
Detection of group A beta-hemolytic Streptococcus employing three different detection methods: culture, rapid antigen detecting test, and molecular assay |
title_fullStr |
Detection of group A beta-hemolytic Streptococcus employing three different detection methods: culture, rapid antigen detecting test, and molecular assay |
title_full_unstemmed |
Detection of group A beta-hemolytic Streptococcus employing three different detection methods: culture, rapid antigen detecting test, and molecular assay |
title_sort |
Detection of group A beta-hemolytic Streptococcus employing three different detection methods: culture, rapid antigen detecting test, and molecular assay |
author |
Santos, Odimara [UNIFESP] |
author_facet |
Santos, Odimara [UNIFESP] Weckx, Luc Louis Maurice [UNIFESP] Pignatari, Antonio Carlos Campos [UNIFESP] Pignatari, Shirley Shizue Nagata [UNIFESP] |
author_role |
author |
author2 |
Weckx, Luc Louis Maurice [UNIFESP] Pignatari, Antonio Carlos Campos [UNIFESP] Pignatari, Shirley Shizue Nagata [UNIFESP] |
author2_role |
author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Santos, Odimara [UNIFESP] Weckx, Luc Louis Maurice [UNIFESP] Pignatari, Antonio Carlos Campos [UNIFESP] Pignatari, Shirley Shizue Nagata [UNIFESP] |
dc.subject.eng.fl_str_mv |
Acute pharyngotonsillitis GABHS laboratory detection |
topic |
Acute pharyngotonsillitis GABHS laboratory detection |
description |
In order to study the prevalence of Group A beta-hemolytic Streptococcus (GABHS) pharyngotonsillitis in our pediatric population and to compare different sampling methods of GABHS detection, oropharyngeal swabs from 50 children with acute pharyngotonsillitis, between 1 and 12 years old, were used simultaneously for culture, molecular assay and rapid GABHS antigen detection tests. All children were clinically examined at the Division of Pediatric Otorhinolaryngology of the Federal University of São Paulo. Diagnostic criteria were based on signs and symptoms, including sore throat, fever and oropharyngeal purulent secretion. Children that had been treated with antibiotics were excluded. Overall, combining the three methods, the prevalence of GABHS was 34%. GABHS was diagnosed in 30% of the bacterial cultures, in 25% of the samples tested with the molecular nucleic acid hybridization method and in 26% of the cases tested with the rapid antigen detection test. There was no significant difference between these three methods. |
publishDate |
2003 |
dc.date.issued.fl_str_mv |
2003-10-01 |
dc.date.accessioned.fl_str_mv |
2015-06-14T13:30:10Z |
dc.date.available.fl_str_mv |
2015-06-14T13:30:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 7, n. 5, p. 297-300, 2003. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/1885 http://dx.doi.org/10.1590/S1413-86702003000500003 |
dc.identifier.issn.none.fl_str_mv |
1413-8670 |
dc.identifier.file.none.fl_str_mv |
S1413-86702003000500003.pdf |
dc.identifier.scielo.none.fl_str_mv |
S1413-86702003000500003 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S1413-86702003000500003 |
identifier_str_mv |
Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 7, n. 5, p. 297-300, 2003. 1413-8670 S1413-86702003000500003.pdf S1413-86702003000500003 10.1590/S1413-86702003000500003 |
url |
http://repositorio.unifesp.br/handle/11600/1885 http://dx.doi.org/10.1590/S1413-86702003000500003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Brazilian Journal of Infectious Diseases |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
297-300 |
dc.publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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Universidade Federal de São Paulo (UNIFESP) |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP |
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