Microbial community structure and dynamics in thermophilic composting viewed through metagenomics and metatranscriptomics

• Autor(a) principal: Antunes, Luciana Principal
• Data de Publicação: 2016
• Outros Autores: Martins, Layla Farage, Pereira, Roberta Verciano, Thomas, Andrew Maltez, Barbosa, Deibs, Lemos, Leandro Nascimento, Machado Silva, Gianluca Major, Silva Moura, Livia Maria, Condomitti Epamino, George Willian, Digiampietri, Luciano Antonio, Lombardi, Karen Cristina, Ramos, Patricia Locosque, Quaggio, Ronaldo Bento, Oliveira, Julio Cezar Franco de [UNIFESP], Pascon, Renata Castiglioni [UNIFESP], da Cruz, Joao Batista, da Silva, Aline Maria, Setubal, Joao Carlos
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.1038/srep38915; https://repositorio.unifesp.br/handle/11600/56552

Composting is a promising source of new organisms and thermostable enzymes that may be helpful in environmental management and industrial processes. Here we present results of metagenomicand metatranscriptomic-based analyses of a large composting operation in the Sao Paulo Zoo Park. This composting exhibits a sustained thermophilic profile (50 degrees C to 75 degrees C), which seems to preclude fungal activity. The main novelty of our study is the combination of time-series sampling with shotgun DNA, 16S rRNA gene amplicon, and metatranscriptome high-throughput sequencing, enabling an unprecedented detailed view of microbial community structure, dynamics, and function in this ecosystem. The time-series data showed that the turning procedure has a strong impact on the compost microbiota, restoring to a certain extent the population profile seen at the beginning of the process