Microbial composition of inoculum and mature compost in the São Paulo Zoo composting process assessed through metagenomics

• Autor(a) principal: Guima, Suzana Eiko Sato
• Data de Publicação: 2021
• Tipo de documento: Dissertação
• Idioma: eng
• Título da fonte: Biblioteca Digital de Teses e Dissertações da USP
• Texto Completo: https://www.teses.usp.br/teses/disponiveis/95/95131/tde-16022021-122522/
• topic: 16S rRNA; Degradação; Degradation; Genomas montados a partir de metagenomas; Lignocellulose; Lignocellulose; MAGs; MAGs; Metagenome-assembled genomes; rRNA 16S

Composting piles consist in a thermophilic environment that can contain bacteria and enzymes potentially related to lignocellulosic deconstruction. Inoculum is the composting material in advanced stage added to the organic matter to be decomposed, while mature compost is the final product of composting product ready to be used as fertilizer. Here, we present results of 16S rRNA profile and metagenome-assembled genomes (MAGs) analysis from inoculum and mature compost samples collected from a composting facility at the São Paulo Zoo Park. We were able to recover 17 MAGs from inoculum samples and 16 MAGs from mature compost samples. Variation in abundance over time shows that these MAGs are likely present during the whole composting process, even if they are present in low abundance. Groups of MAGs can contribute to the lignocellulosic deconstruction in different stages of composting. Genes encoding cellulases, endohemicellulases, and oligosaccharide-degrading enzymes were distributed in MAGs from both inoculum and mature compost samples. Auxiliary activity enzymes were found especially in MAGs recovered from mature compost samples. Mature compost material can contain bacteria that play an important role to the lignin degradation, and as such they can be potential candidates for lignocellulose degradation biotechnological processes.