Keratinocyte growth factor protected cultured human keratinocytes exposed to oxidative stress

Detalhes bibliográficos
Autor(a) principal: Gragnani, Alfredo [UNIFESP]
Data de Publicação: 2010
Outros Autores: Rossi, Marina Bertelli [UNIFESP], Albuquerque, Janne Cely Medeiros [UNIFESP], Brito, Gabriela Soares Silva [UNIFESP], Aloise, Antonio Carlos [UNIFESP], Ferreira, Lydia Masako [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0102-86502010000100019
http://repositorio.unifesp.br/handle/11600/5594
Resumo: PURPOSE: To evaluate effects of oxidative stress and supplementation of keratinocyte growth factor (KGF) on cultivated human keratinocytes. METHODS: Oxidative stress was produced through addition of hydrogen peroxide (H2O2) to the culture medium. Cultivated human keratinocytes were divided in 4 groups: Group control (G C), Group KGF (G KGF), Group H2O2 (G H2O2), Group H2O2 and KGF (G H2O2-KGF). Each experiment was accomplished with the same lineage cultivated keratinocytes, in triplicate. Cell viability was evaluated by trypan blue exclusion assay. RESULTS: The results showed that the culture medium supplemented with KGF presented a small rate of cell viability when compared to cells only in culture medium (p<0,001). It demonstrated that only the growth factor does not have protector effects for cells in vitro. However, in front of the oxidative stress produced by addition of hydrogen peroxide to the medium, KGF showed a beneficial effect, protecting cells when compared to the group that suffered hydrogen peroxide action but had not been exposed to KGF (p<0,001). CONCLUSION: KGF determined protection to the primary human keratinocytes exposed to oxidative stress.
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spelling Keratinocyte growth factor protected cultured human keratinocytes exposed to oxidative stressFator de crescimento de queratinócitos protegeu queratinócitos humanos cultivados expostos ao estresse oxidativoKeratinocytesCell SurvivalOxidative StressCeratinócitosSobrevivência CelularEstresse OxidativoPURPOSE: To evaluate effects of oxidative stress and supplementation of keratinocyte growth factor (KGF) on cultivated human keratinocytes. METHODS: Oxidative stress was produced through addition of hydrogen peroxide (H2O2) to the culture medium. Cultivated human keratinocytes were divided in 4 groups: Group control (G C), Group KGF (G KGF), Group H2O2 (G H2O2), Group H2O2 and KGF (G H2O2-KGF). Each experiment was accomplished with the same lineage cultivated keratinocytes, in triplicate. Cell viability was evaluated by trypan blue exclusion assay. RESULTS: The results showed that the culture medium supplemented with KGF presented a small rate of cell viability when compared to cells only in culture medium (p<0,001). It demonstrated that only the growth factor does not have protector effects for cells in vitro. However, in front of the oxidative stress produced by addition of hydrogen peroxide to the medium, KGF showed a beneficial effect, protecting cells when compared to the group that suffered hydrogen peroxide action but had not been exposed to KGF (p<0,001). CONCLUSION: KGF determined protection to the primary human keratinocytes exposed to oxidative stress.OBJETIVO: Avaliar os efeitos do estresse oxidativo e da suplementação do fator de crescimento de queratinócitos (KGF) em queratinócitos humanos cultivados. MÉTODOS: O estresse oxidativo foi produzido através da adição de peróxido de hidrogênio (H2O2) ao meio de cultura. Os queratinócitos humanos cultivados foram divididos em quatro grupos: grupo controle (G C), grupo KGF (G KGF), grupo H2O2 (G H2O2), grupo H2O2 e KGF (G H2O2-KGF). Cada experimento foi realizado com a mesma linhagem celular, em triplicata. A viabilidade celular foi avaliada pelo ensaio da exclusão do azul de tripan. RESULTADOS: Os resultados mostraram que o meio de cultura suplementado com o KGF apresentou menor taxa de viabilidade celular quando comparado às células do grupo controle (p<0,001). Isso mostra que somente o fator de crescimento de queratinócitos não apresentou efeito protetor às células em cultura. Entretanto, frente ao estresse oxidativo produzido pela adição do peróxido de hidrogênio ao meio de cultura, o KGF mostrou efeito benéfico, protegendo as células quando comparado ao grupo que sofreu a ação do estresse oxidativo, mas que não foi exposta ao KGF (p<0,001). CONCLUSÃO: O KGF determinou a proteção aos queratinócitos humanos primários cultivados expostos ao estresse oxidativo.UNIFESP Plastic Surgery DivisionUNIFESPUNIFESP Plastic Surgery Post-graduation ProgramUNIFESP, Plastic Surgery DivisionUNIFESP, Plastic Surgery Post-graduation ProgramSciELOConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Sociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaUniversidade Federal de São Paulo (UNIFESP)Gragnani, Alfredo [UNIFESP]Rossi, Marina Bertelli [UNIFESP]Albuquerque, Janne Cely Medeiros [UNIFESP]Brito, Gabriela Soares Silva [UNIFESP]Aloise, Antonio Carlos [UNIFESP]Ferreira, Lydia Masako [UNIFESP]2015-06-14T13:41:32Z2015-06-14T13:41:32Z2010-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion93-97application/pdfhttp://dx.doi.org/10.1590/S0102-86502010000100019Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 25, n. 1, p. 93-97, 2010.10.1590/S0102-86502010000100019S0102-86502010000100019.pdf0102-8650S0102-86502010000100019http://repositorio.unifesp.br/handle/11600/5594WOS:000275423000018engActa Cirurgica Brasileirainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-04T23:53:43Zoai:repositorio.unifesp.br/:11600/5594Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-04T23:53:43Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Keratinocyte growth factor protected cultured human keratinocytes exposed to oxidative stress
Fator de crescimento de queratinócitos protegeu queratinócitos humanos cultivados expostos ao estresse oxidativo
title Keratinocyte growth factor protected cultured human keratinocytes exposed to oxidative stress
spellingShingle Keratinocyte growth factor protected cultured human keratinocytes exposed to oxidative stress
Gragnani, Alfredo [UNIFESP]
Keratinocytes
Cell Survival
Oxidative Stress
Ceratinócitos
Sobrevivência Celular
Estresse Oxidativo
title_short Keratinocyte growth factor protected cultured human keratinocytes exposed to oxidative stress
title_full Keratinocyte growth factor protected cultured human keratinocytes exposed to oxidative stress
title_fullStr Keratinocyte growth factor protected cultured human keratinocytes exposed to oxidative stress
title_full_unstemmed Keratinocyte growth factor protected cultured human keratinocytes exposed to oxidative stress
title_sort Keratinocyte growth factor protected cultured human keratinocytes exposed to oxidative stress
author Gragnani, Alfredo [UNIFESP]
author_facet Gragnani, Alfredo [UNIFESP]
Rossi, Marina Bertelli [UNIFESP]
Albuquerque, Janne Cely Medeiros [UNIFESP]
Brito, Gabriela Soares Silva [UNIFESP]
Aloise, Antonio Carlos [UNIFESP]
Ferreira, Lydia Masako [UNIFESP]
author_role author
author2 Rossi, Marina Bertelli [UNIFESP]
Albuquerque, Janne Cely Medeiros [UNIFESP]
Brito, Gabriela Soares Silva [UNIFESP]
Aloise, Antonio Carlos [UNIFESP]
Ferreira, Lydia Masako [UNIFESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Gragnani, Alfredo [UNIFESP]
Rossi, Marina Bertelli [UNIFESP]
Albuquerque, Janne Cely Medeiros [UNIFESP]
Brito, Gabriela Soares Silva [UNIFESP]
Aloise, Antonio Carlos [UNIFESP]
Ferreira, Lydia Masako [UNIFESP]
dc.subject.por.fl_str_mv Keratinocytes
Cell Survival
Oxidative Stress
Ceratinócitos
Sobrevivência Celular
Estresse Oxidativo
topic Keratinocytes
Cell Survival
Oxidative Stress
Ceratinócitos
Sobrevivência Celular
Estresse Oxidativo
description PURPOSE: To evaluate effects of oxidative stress and supplementation of keratinocyte growth factor (KGF) on cultivated human keratinocytes. METHODS: Oxidative stress was produced through addition of hydrogen peroxide (H2O2) to the culture medium. Cultivated human keratinocytes were divided in 4 groups: Group control (G C), Group KGF (G KGF), Group H2O2 (G H2O2), Group H2O2 and KGF (G H2O2-KGF). Each experiment was accomplished with the same lineage cultivated keratinocytes, in triplicate. Cell viability was evaluated by trypan blue exclusion assay. RESULTS: The results showed that the culture medium supplemented with KGF presented a small rate of cell viability when compared to cells only in culture medium (p<0,001). It demonstrated that only the growth factor does not have protector effects for cells in vitro. However, in front of the oxidative stress produced by addition of hydrogen peroxide to the medium, KGF showed a beneficial effect, protecting cells when compared to the group that suffered hydrogen peroxide action but had not been exposed to KGF (p<0,001). CONCLUSION: KGF determined protection to the primary human keratinocytes exposed to oxidative stress.
publishDate 2010
dc.date.none.fl_str_mv 2010-02-01
2015-06-14T13:41:32Z
2015-06-14T13:41:32Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0102-86502010000100019
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 25, n. 1, p. 93-97, 2010.
10.1590/S0102-86502010000100019
S0102-86502010000100019.pdf
0102-8650
S0102-86502010000100019
http://repositorio.unifesp.br/handle/11600/5594
WOS:000275423000018
url http://dx.doi.org/10.1590/S0102-86502010000100019
http://repositorio.unifesp.br/handle/11600/5594
identifier_str_mv Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 25, n. 1, p. 93-97, 2010.
10.1590/S0102-86502010000100019
S0102-86502010000100019.pdf
0102-8650
S0102-86502010000100019
WOS:000275423000018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Cirurgica Brasileira
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 93-97
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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