Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats

Detalhes bibliográficos
Autor(a) principal: Le Sueur-Maluf, Luciana [UNIFESP]
Data de Publicação: 2015
Outros Autores: Viana, Milena de Barros [UNIFESP], Nagaoka, Márcia Regina [UNIFESP], Amorim, Ana Laura B., Cardoso, Amanda N., Rodrigues, Bruna C., Mendes, Natália F., Bittencourt, Jackson C., Céspedes, Isabel Cristina [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/0001-3765201420130262
http://repositorio.unifesp.br/handle/11600/8783
Resumo: Hepatic encephalopathy (HE) encompasses a variety of neuropsychiatric symptoms, including anxiety and psychomotor dysfunction. Although HE is a frequent complication of liver cirrhosis, the neurobiological substrates responsible for its clinical manifestations are largely unclear. In the present study, male Wistar rats were bile duct-ligated (BDL), a procedure which induces liver cirrhosis, and on the 21st day after surgery tested in the elevated plus-maze (EPM) and in an open field for anxiety and locomotor activity measurements. Analysis of Fos protein immunoreactivity (Fos-ir) was used to better understand the neurobiological alterations present in BDL animals. Plasma levels of ammonia were quantified and histopathological analysis of the livers was performed. BDL rats showed a significant decrease in the percentage of entries and time spent in the open arms of the EPM, an anxiogenic effect. These animals also presented significant decreases in Fos-ir in the lateral septal nucleus and medial amygdalar nucleus. Their ammonia plasma levels were significantly higher when compared to the sham group and the diagnosis of cirrhosis was confirmed by histopathological analysis. These results indicate that the BDL model induces anxiogenic results, possibly related to changes in the activation of anxiety-mediating circuitries and to increases in ammonia plasma levels.
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spelling Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic ratsanxietybile-duct ligationFos-immunoreactivityhepatic encephalopathylocomotor activityansiedadeligação do ducto biliarencefalopatia hepáticaatividade locomotoraHepatic encephalopathy (HE) encompasses a variety of neuropsychiatric symptoms, including anxiety and psychomotor dysfunction. Although HE is a frequent complication of liver cirrhosis, the neurobiological substrates responsible for its clinical manifestations are largely unclear. In the present study, male Wistar rats were bile duct-ligated (BDL), a procedure which induces liver cirrhosis, and on the 21st day after surgery tested in the elevated plus-maze (EPM) and in an open field for anxiety and locomotor activity measurements. Analysis of Fos protein immunoreactivity (Fos-ir) was used to better understand the neurobiological alterations present in BDL animals. Plasma levels of ammonia were quantified and histopathological analysis of the livers was performed. BDL rats showed a significant decrease in the percentage of entries and time spent in the open arms of the EPM, an anxiogenic effect. These animals also presented significant decreases in Fos-ir in the lateral septal nucleus and medial amygdalar nucleus. Their ammonia plasma levels were significantly higher when compared to the sham group and the diagnosis of cirrhosis was confirmed by histopathological analysis. These results indicate that the BDL model induces anxiogenic results, possibly related to changes in the activation of anxiety-mediating circuitries and to increases in ammonia plasma levels.A Encefalopatia hepática (HE) engloba uma variedade de sintomas neuropsiquiátricos, incluindo ansiedade e disfunção psicomotora. Embora seja uma complicação frequente da cirrose hepática, os substratos neurobiológicos responsáveis por suas manifestações clínicas são em grande parte desconhecidos. No presente estudo, ratos Wistar machos foram submetidos ao procedimento cirúrgico de ligação e secção do ducto biliar (BDL; bile-duct ligation), para indução da cirrose hepática e, no 21º dia após a cirurgia, submetidos aos testes comportamentais no labirinto em cruz elevado (LCE) e campo aberto para avaliação da ansiedade e atividade locomotora. A análise da imunorreatividade à proteína Fos (Fos-ir) foi utilizada para melhor compreender as alterações neurobiológicas presentes nos animais do grupo BDL. Foi realizada a quantificação da concentração de amônia plasmática e análise histopatológica dos fígados. Os ratos do grupo BDL mostraram diminuição significativa na porcentagem de entradas e tempo gasto nos braços abertos do LCE, caracterizando efeito ansiogênico. Estes animais também apresentaram redução significativa na Fos-ir no núcleo septal lateral e núcleo medial da amígdala. A concentração plasmática de amônia foi significativamente mais elevada que a do grupo sham e o diagnóstico de cirrose foi confirmado por análise histopatológica. Estes resultados indicam que o modelo de HE induzido por BDL induz efeito ansiogênico possivelmente relacionado à ativação de circuitos mediadores da ansiedade e à hiperamonemia.Universidade Federal de São Paulo (UNIFESP) Departamento de BiociênciasUniversidade de São Paulo Instituto de Ciências Biomédicas Departamento de AnatomiaUNIFESP, Depto. de BiociênciasSciELOAcademia Brasileira de CiênciasUniversidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Le Sueur-Maluf, Luciana [UNIFESP]Viana, Milena de Barros [UNIFESP]Nagaoka, Márcia Regina [UNIFESP]Amorim, Ana Laura B.Cardoso, Amanda N.Rodrigues, Bruna C.Mendes, Natália F.Bittencourt, Jackson C.Céspedes, Isabel Cristina [UNIFESP]2015-06-14T13:47:30Z2015-06-14T13:47:30Z2015-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://dx.doi.org/10.1590/0001-3765201420130262Anais da Academia Brasileira de Ciências. Academia Brasileira de Ciências, v. 00, n. ahead, p. 00-00, 2015.10.1590/0001-3765201420130262S0001-37652015005030262.pdf0001-3765S0001-37652015005030262http://repositorio.unifesp.br/handle/11600/8783engAnais da Academia Brasileira de Ciênciasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-05T14:19:59Zoai:repositorio.unifesp.br/:11600/8783Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-05T14:19:59Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats
title Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats
spellingShingle Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats
Le Sueur-Maluf, Luciana [UNIFESP]
anxiety
bile-duct ligation
Fos-immunoreactivity
hepatic encephalopathy
locomotor activity
ansiedade
ligação do ducto biliar
encefalopatia hepática
atividade locomotora
title_short Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats
title_full Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats
title_fullStr Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats
title_full_unstemmed Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats
title_sort Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats
author Le Sueur-Maluf, Luciana [UNIFESP]
author_facet Le Sueur-Maluf, Luciana [UNIFESP]
Viana, Milena de Barros [UNIFESP]
Nagaoka, Márcia Regina [UNIFESP]
Amorim, Ana Laura B.
Cardoso, Amanda N.
Rodrigues, Bruna C.
Mendes, Natália F.
Bittencourt, Jackson C.
Céspedes, Isabel Cristina [UNIFESP]
author_role author
author2 Viana, Milena de Barros [UNIFESP]
Nagaoka, Márcia Regina [UNIFESP]
Amorim, Ana Laura B.
Cardoso, Amanda N.
Rodrigues, Bruna C.
Mendes, Natália F.
Bittencourt, Jackson C.
Céspedes, Isabel Cristina [UNIFESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Le Sueur-Maluf, Luciana [UNIFESP]
Viana, Milena de Barros [UNIFESP]
Nagaoka, Márcia Regina [UNIFESP]
Amorim, Ana Laura B.
Cardoso, Amanda N.
Rodrigues, Bruna C.
Mendes, Natália F.
Bittencourt, Jackson C.
Céspedes, Isabel Cristina [UNIFESP]
dc.subject.por.fl_str_mv anxiety
bile-duct ligation
Fos-immunoreactivity
hepatic encephalopathy
locomotor activity
ansiedade
ligação do ducto biliar
encefalopatia hepática
atividade locomotora
topic anxiety
bile-duct ligation
Fos-immunoreactivity
hepatic encephalopathy
locomotor activity
ansiedade
ligação do ducto biliar
encefalopatia hepática
atividade locomotora
description Hepatic encephalopathy (HE) encompasses a variety of neuropsychiatric symptoms, including anxiety and psychomotor dysfunction. Although HE is a frequent complication of liver cirrhosis, the neurobiological substrates responsible for its clinical manifestations are largely unclear. In the present study, male Wistar rats were bile duct-ligated (BDL), a procedure which induces liver cirrhosis, and on the 21st day after surgery tested in the elevated plus-maze (EPM) and in an open field for anxiety and locomotor activity measurements. Analysis of Fos protein immunoreactivity (Fos-ir) was used to better understand the neurobiological alterations present in BDL animals. Plasma levels of ammonia were quantified and histopathological analysis of the livers was performed. BDL rats showed a significant decrease in the percentage of entries and time spent in the open arms of the EPM, an anxiogenic effect. These animals also presented significant decreases in Fos-ir in the lateral septal nucleus and medial amygdalar nucleus. Their ammonia plasma levels were significantly higher when compared to the sham group and the diagnosis of cirrhosis was confirmed by histopathological analysis. These results indicate that the BDL model induces anxiogenic results, possibly related to changes in the activation of anxiety-mediating circuitries and to increases in ammonia plasma levels.
publishDate 2015
dc.date.none.fl_str_mv 2015-06-14T13:47:30Z
2015-06-14T13:47:30Z
2015-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/0001-3765201420130262
Anais da Academia Brasileira de Ciências. Academia Brasileira de Ciências, v. 00, n. ahead, p. 00-00, 2015.
10.1590/0001-3765201420130262
S0001-37652015005030262.pdf
0001-3765
S0001-37652015005030262
http://repositorio.unifesp.br/handle/11600/8783
url http://dx.doi.org/10.1590/0001-3765201420130262
http://repositorio.unifesp.br/handle/11600/8783
identifier_str_mv Anais da Academia Brasileira de Ciências. Academia Brasileira de Ciências, v. 00, n. ahead, p. 00-00, 2015.
10.1590/0001-3765201420130262
S0001-37652015005030262.pdf
0001-3765
S0001-37652015005030262
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Anais da Academia Brasileira de Ciências
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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