Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1590/0001-3765201420130262 http://repositorio.unifesp.br/handle/11600/8783 |
Resumo: | Hepatic encephalopathy (HE) encompasses a variety of neuropsychiatric symptoms, including anxiety and psychomotor dysfunction. Although HE is a frequent complication of liver cirrhosis, the neurobiological substrates responsible for its clinical manifestations are largely unclear. In the present study, male Wistar rats were bile duct-ligated (BDL), a procedure which induces liver cirrhosis, and on the 21st day after surgery tested in the elevated plus-maze (EPM) and in an open field for anxiety and locomotor activity measurements. Analysis of Fos protein immunoreactivity (Fos-ir) was used to better understand the neurobiological alterations present in BDL animals. Plasma levels of ammonia were quantified and histopathological analysis of the livers was performed. BDL rats showed a significant decrease in the percentage of entries and time spent in the open arms of the EPM, an anxiogenic effect. These animals also presented significant decreases in Fos-ir in the lateral septal nucleus and medial amygdalar nucleus. Their ammonia plasma levels were significantly higher when compared to the sham group and the diagnosis of cirrhosis was confirmed by histopathological analysis. These results indicate that the BDL model induces anxiogenic results, possibly related to changes in the activation of anxiety-mediating circuitries and to increases in ammonia plasma levels. |
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Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic ratsanxietybile-duct ligationFos-immunoreactivityhepatic encephalopathylocomotor activityansiedadeligação do ducto biliarencefalopatia hepáticaatividade locomotoraHepatic encephalopathy (HE) encompasses a variety of neuropsychiatric symptoms, including anxiety and psychomotor dysfunction. Although HE is a frequent complication of liver cirrhosis, the neurobiological substrates responsible for its clinical manifestations are largely unclear. In the present study, male Wistar rats were bile duct-ligated (BDL), a procedure which induces liver cirrhosis, and on the 21st day after surgery tested in the elevated plus-maze (EPM) and in an open field for anxiety and locomotor activity measurements. Analysis of Fos protein immunoreactivity (Fos-ir) was used to better understand the neurobiological alterations present in BDL animals. Plasma levels of ammonia were quantified and histopathological analysis of the livers was performed. BDL rats showed a significant decrease in the percentage of entries and time spent in the open arms of the EPM, an anxiogenic effect. These animals also presented significant decreases in Fos-ir in the lateral septal nucleus and medial amygdalar nucleus. Their ammonia plasma levels were significantly higher when compared to the sham group and the diagnosis of cirrhosis was confirmed by histopathological analysis. These results indicate that the BDL model induces anxiogenic results, possibly related to changes in the activation of anxiety-mediating circuitries and to increases in ammonia plasma levels.A Encefalopatia hepática (HE) engloba uma variedade de sintomas neuropsiquiátricos, incluindo ansiedade e disfunção psicomotora. Embora seja uma complicação frequente da cirrose hepática, os substratos neurobiológicos responsáveis por suas manifestações clínicas são em grande parte desconhecidos. No presente estudo, ratos Wistar machos foram submetidos ao procedimento cirúrgico de ligação e secção do ducto biliar (BDL; bile-duct ligation), para indução da cirrose hepática e, no 21º dia após a cirurgia, submetidos aos testes comportamentais no labirinto em cruz elevado (LCE) e campo aberto para avaliação da ansiedade e atividade locomotora. A análise da imunorreatividade à proteína Fos (Fos-ir) foi utilizada para melhor compreender as alterações neurobiológicas presentes nos animais do grupo BDL. Foi realizada a quantificação da concentração de amônia plasmática e análise histopatológica dos fígados. Os ratos do grupo BDL mostraram diminuição significativa na porcentagem de entradas e tempo gasto nos braços abertos do LCE, caracterizando efeito ansiogênico. Estes animais também apresentaram redução significativa na Fos-ir no núcleo septal lateral e núcleo medial da amígdala. A concentração plasmática de amônia foi significativamente mais elevada que a do grupo sham e o diagnóstico de cirrose foi confirmado por análise histopatológica. Estes resultados indicam que o modelo de HE induzido por BDL induz efeito ansiogênico possivelmente relacionado à ativação de circuitos mediadores da ansiedade e à hiperamonemia.Universidade Federal de São Paulo (UNIFESP) Departamento de BiociênciasUniversidade de São Paulo Instituto de Ciências Biomédicas Departamento de AnatomiaUNIFESP, Depto. de BiociênciasSciELOAcademia Brasileira de CiênciasUniversidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Le Sueur-Maluf, Luciana [UNIFESP]Viana, Milena de Barros [UNIFESP]Nagaoka, Márcia Regina [UNIFESP]Amorim, Ana Laura B.Cardoso, Amanda N.Rodrigues, Bruna C.Mendes, Natália F.Bittencourt, Jackson C.Céspedes, Isabel Cristina [UNIFESP]2015-06-14T13:47:30Z2015-06-14T13:47:30Z2015-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://dx.doi.org/10.1590/0001-3765201420130262Anais da Academia Brasileira de Ciências. Academia Brasileira de Ciências, v. 00, n. ahead, p. 00-00, 2015.10.1590/0001-3765201420130262S0001-37652015005030262.pdf0001-3765S0001-37652015005030262http://repositorio.unifesp.br/handle/11600/8783engAnais da Academia Brasileira de Ciênciasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-05T14:19:59Zoai:repositorio.unifesp.br/:11600/8783Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-05T14:19:59Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats |
title |
Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats |
spellingShingle |
Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats Le Sueur-Maluf, Luciana [UNIFESP] anxiety bile-duct ligation Fos-immunoreactivity hepatic encephalopathy locomotor activity ansiedade ligação do ducto biliar encefalopatia hepática atividade locomotora |
title_short |
Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats |
title_full |
Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats |
title_fullStr |
Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats |
title_full_unstemmed |
Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats |
title_sort |
Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats |
author |
Le Sueur-Maluf, Luciana [UNIFESP] |
author_facet |
Le Sueur-Maluf, Luciana [UNIFESP] Viana, Milena de Barros [UNIFESP] Nagaoka, Márcia Regina [UNIFESP] Amorim, Ana Laura B. Cardoso, Amanda N. Rodrigues, Bruna C. Mendes, Natália F. Bittencourt, Jackson C. Céspedes, Isabel Cristina [UNIFESP] |
author_role |
author |
author2 |
Viana, Milena de Barros [UNIFESP] Nagaoka, Márcia Regina [UNIFESP] Amorim, Ana Laura B. Cardoso, Amanda N. Rodrigues, Bruna C. Mendes, Natália F. Bittencourt, Jackson C. Céspedes, Isabel Cristina [UNIFESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Le Sueur-Maluf, Luciana [UNIFESP] Viana, Milena de Barros [UNIFESP] Nagaoka, Márcia Regina [UNIFESP] Amorim, Ana Laura B. Cardoso, Amanda N. Rodrigues, Bruna C. Mendes, Natália F. Bittencourt, Jackson C. Céspedes, Isabel Cristina [UNIFESP] |
dc.subject.por.fl_str_mv |
anxiety bile-duct ligation Fos-immunoreactivity hepatic encephalopathy locomotor activity ansiedade ligação do ducto biliar encefalopatia hepática atividade locomotora |
topic |
anxiety bile-duct ligation Fos-immunoreactivity hepatic encephalopathy locomotor activity ansiedade ligação do ducto biliar encefalopatia hepática atividade locomotora |
description |
Hepatic encephalopathy (HE) encompasses a variety of neuropsychiatric symptoms, including anxiety and psychomotor dysfunction. Although HE is a frequent complication of liver cirrhosis, the neurobiological substrates responsible for its clinical manifestations are largely unclear. In the present study, male Wistar rats were bile duct-ligated (BDL), a procedure which induces liver cirrhosis, and on the 21st day after surgery tested in the elevated plus-maze (EPM) and in an open field for anxiety and locomotor activity measurements. Analysis of Fos protein immunoreactivity (Fos-ir) was used to better understand the neurobiological alterations present in BDL animals. Plasma levels of ammonia were quantified and histopathological analysis of the livers was performed. BDL rats showed a significant decrease in the percentage of entries and time spent in the open arms of the EPM, an anxiogenic effect. These animals also presented significant decreases in Fos-ir in the lateral septal nucleus and medial amygdalar nucleus. Their ammonia plasma levels were significantly higher when compared to the sham group and the diagnosis of cirrhosis was confirmed by histopathological analysis. These results indicate that the BDL model induces anxiogenic results, possibly related to changes in the activation of anxiety-mediating circuitries and to increases in ammonia plasma levels. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-06-14T13:47:30Z 2015-06-14T13:47:30Z 2015-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0001-3765201420130262 Anais da Academia Brasileira de Ciências. Academia Brasileira de Ciências, v. 00, n. ahead, p. 00-00, 2015. 10.1590/0001-3765201420130262 S0001-37652015005030262.pdf 0001-3765 S0001-37652015005030262 http://repositorio.unifesp.br/handle/11600/8783 |
url |
http://dx.doi.org/10.1590/0001-3765201420130262 http://repositorio.unifesp.br/handle/11600/8783 |
identifier_str_mv |
Anais da Academia Brasileira de Ciências. Academia Brasileira de Ciências, v. 00, n. ahead, p. 00-00, 2015. 10.1590/0001-3765201420130262 S0001-37652015005030262.pdf 0001-3765 S0001-37652015005030262 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Anais da Academia Brasileira de Ciências |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
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1814268441519980544 |