Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Anais da Academia Brasileira de Ciências (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100331 |
Resumo: | Hepatic encephalopathy (HE) encompasses a variety of neuropsychiatric symptoms, including anxiety and psychomotor dysfunction. Although HE is a frequent complication of liver cirrhosis, the neurobiological substrates responsible for its clinical manifestations are largely unclear. In the present study, male Wistar rats were bile duct-ligated (BDL), a procedure which induces liver cirrhosis, and on the 21st day after surgery tested in the elevated plus-maze (EPM) and in an open field for anxiety and locomotor activity measurements. Analysis of Fos protein immunoreactivity (Fos-ir) was used to better understand the neurobiological alterations present in BDL animals. Plasma levels of ammonia were quantified and histopathological analysis of the livers was performed. BDL rats showed a significant decrease in the percentage of entries and time spent in the open arms of the EPM, an anxiogenic effect. These animals also presented significant decreases in Fos-ir in the lateral septal nucleus and medial amygdalar nucleus. Their ammonia plasma levels were significantly higher when compared to the sham group and the diagnosis of cirrhosis was confirmed by histopathological analysis. These results indicate that the BDL model induces anxiogenic results, possibly related to changes in the activation of anxiety-mediating circuitries and to increases in ammonia plasma levels. |
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Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic ratsanxietybile-duct ligationFos-immunoreactivityhepatic encephalopathylocomotor activityHepatic encephalopathy (HE) encompasses a variety of neuropsychiatric symptoms, including anxiety and psychomotor dysfunction. Although HE is a frequent complication of liver cirrhosis, the neurobiological substrates responsible for its clinical manifestations are largely unclear. In the present study, male Wistar rats were bile duct-ligated (BDL), a procedure which induces liver cirrhosis, and on the 21st day after surgery tested in the elevated plus-maze (EPM) and in an open field for anxiety and locomotor activity measurements. Analysis of Fos protein immunoreactivity (Fos-ir) was used to better understand the neurobiological alterations present in BDL animals. Plasma levels of ammonia were quantified and histopathological analysis of the livers was performed. BDL rats showed a significant decrease in the percentage of entries and time spent in the open arms of the EPM, an anxiogenic effect. These animals also presented significant decreases in Fos-ir in the lateral septal nucleus and medial amygdalar nucleus. Their ammonia plasma levels were significantly higher when compared to the sham group and the diagnosis of cirrhosis was confirmed by histopathological analysis. These results indicate that the BDL model induces anxiogenic results, possibly related to changes in the activation of anxiety-mediating circuitries and to increases in ammonia plasma levels.Academia Brasileira de Ciências2015-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100331Anais da Academia Brasileira de Ciências v.87 n.1 2015reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201420130262info:eu-repo/semantics/openAccessSUEUR-MALUF,LUCIANA LEVIANA,MILENA B.NAGAOKA,MÁRCIA R.AMORIM,ANA LAURA B.CARDOSO,AMANDA N.RODRIGUES,BRUNA C.MENDES,NATÁLIA F.BITTENCOURT,JACKSON C.CÉSPEDES,ISABEL C.eng2015-11-16T00:00:00Zoai:scielo:S0001-37652015000100331Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2015-11-16T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false |
dc.title.none.fl_str_mv |
Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats |
title |
Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats |
spellingShingle |
Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats SUEUR-MALUF,LUCIANA LE anxiety bile-duct ligation Fos-immunoreactivity hepatic encephalopathy locomotor activity |
title_short |
Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats |
title_full |
Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats |
title_fullStr |
Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats |
title_full_unstemmed |
Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats |
title_sort |
Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats |
author |
SUEUR-MALUF,LUCIANA LE |
author_facet |
SUEUR-MALUF,LUCIANA LE VIANA,MILENA B. NAGAOKA,MÁRCIA R. AMORIM,ANA LAURA B. CARDOSO,AMANDA N. RODRIGUES,BRUNA C. MENDES,NATÁLIA F. BITTENCOURT,JACKSON C. CÉSPEDES,ISABEL C. |
author_role |
author |
author2 |
VIANA,MILENA B. NAGAOKA,MÁRCIA R. AMORIM,ANA LAURA B. CARDOSO,AMANDA N. RODRIGUES,BRUNA C. MENDES,NATÁLIA F. BITTENCOURT,JACKSON C. CÉSPEDES,ISABEL C. |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
SUEUR-MALUF,LUCIANA LE VIANA,MILENA B. NAGAOKA,MÁRCIA R. AMORIM,ANA LAURA B. CARDOSO,AMANDA N. RODRIGUES,BRUNA C. MENDES,NATÁLIA F. BITTENCOURT,JACKSON C. CÉSPEDES,ISABEL C. |
dc.subject.por.fl_str_mv |
anxiety bile-duct ligation Fos-immunoreactivity hepatic encephalopathy locomotor activity |
topic |
anxiety bile-duct ligation Fos-immunoreactivity hepatic encephalopathy locomotor activity |
description |
Hepatic encephalopathy (HE) encompasses a variety of neuropsychiatric symptoms, including anxiety and psychomotor dysfunction. Although HE is a frequent complication of liver cirrhosis, the neurobiological substrates responsible for its clinical manifestations are largely unclear. In the present study, male Wistar rats were bile duct-ligated (BDL), a procedure which induces liver cirrhosis, and on the 21st day after surgery tested in the elevated plus-maze (EPM) and in an open field for anxiety and locomotor activity measurements. Analysis of Fos protein immunoreactivity (Fos-ir) was used to better understand the neurobiological alterations present in BDL animals. Plasma levels of ammonia were quantified and histopathological analysis of the livers was performed. BDL rats showed a significant decrease in the percentage of entries and time spent in the open arms of the EPM, an anxiogenic effect. These animals also presented significant decreases in Fos-ir in the lateral septal nucleus and medial amygdalar nucleus. Their ammonia plasma levels were significantly higher when compared to the sham group and the diagnosis of cirrhosis was confirmed by histopathological analysis. These results indicate that the BDL model induces anxiogenic results, possibly related to changes in the activation of anxiety-mediating circuitries and to increases in ammonia plasma levels. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100331 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100331 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0001-3765201420130262 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
dc.source.none.fl_str_mv |
Anais da Academia Brasileira de Ciências v.87 n.1 2015 reponame:Anais da Academia Brasileira de Ciências (Online) instname:Academia Brasileira de Ciências (ABC) instacron:ABC |
instname_str |
Academia Brasileira de Ciências (ABC) |
instacron_str |
ABC |
institution |
ABC |
reponame_str |
Anais da Academia Brasileira de Ciências (Online) |
collection |
Anais da Academia Brasileira de Ciências (Online) |
repository.name.fl_str_mv |
Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC) |
repository.mail.fl_str_mv |
||aabc@abc.org.br |
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1754302861514113024 |