Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats

Detalhes bibliográficos
Autor(a) principal: SUEUR-MALUF,LUCIANA LE
Data de Publicação: 2015
Outros Autores: VIANA,MILENA B., NAGAOKA,MÁRCIA R., AMORIM,ANA LAURA B., CARDOSO,AMANDA N., RODRIGUES,BRUNA C., MENDES,NATÁLIA F., BITTENCOURT,JACKSON C., CÉSPEDES,ISABEL C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais da Academia Brasileira de Ciências (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100331
Resumo: Hepatic encephalopathy (HE) encompasses a variety of neuropsychiatric symptoms, including anxiety and psychomotor dysfunction. Although HE is a frequent complication of liver cirrhosis, the neurobiological substrates responsible for its clinical manifestations are largely unclear. In the present study, male Wistar rats were bile duct-ligated (BDL), a procedure which induces liver cirrhosis, and on the 21st day after surgery tested in the elevated plus-maze (EPM) and in an open field for anxiety and locomotor activity measurements. Analysis of Fos protein immunoreactivity (Fos-ir) was used to better understand the neurobiological alterations present in BDL animals. Plasma levels of ammonia were quantified and histopathological analysis of the livers was performed. BDL rats showed a significant decrease in the percentage of entries and time spent in the open arms of the EPM, an anxiogenic effect. These animals also presented significant decreases in Fos-ir in the lateral septal nucleus and medial amygdalar nucleus. Their ammonia plasma levels were significantly higher when compared to the sham group and the diagnosis of cirrhosis was confirmed by histopathological analysis. These results indicate that the BDL model induces anxiogenic results, possibly related to changes in the activation of anxiety-mediating circuitries and to increases in ammonia plasma levels.
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spelling Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic ratsanxietybile-duct ligationFos-immunoreactivityhepatic encephalopathylocomotor activityHepatic encephalopathy (HE) encompasses a variety of neuropsychiatric symptoms, including anxiety and psychomotor dysfunction. Although HE is a frequent complication of liver cirrhosis, the neurobiological substrates responsible for its clinical manifestations are largely unclear. In the present study, male Wistar rats were bile duct-ligated (BDL), a procedure which induces liver cirrhosis, and on the 21st day after surgery tested in the elevated plus-maze (EPM) and in an open field for anxiety and locomotor activity measurements. Analysis of Fos protein immunoreactivity (Fos-ir) was used to better understand the neurobiological alterations present in BDL animals. Plasma levels of ammonia were quantified and histopathological analysis of the livers was performed. BDL rats showed a significant decrease in the percentage of entries and time spent in the open arms of the EPM, an anxiogenic effect. These animals also presented significant decreases in Fos-ir in the lateral septal nucleus and medial amygdalar nucleus. Their ammonia plasma levels were significantly higher when compared to the sham group and the diagnosis of cirrhosis was confirmed by histopathological analysis. These results indicate that the BDL model induces anxiogenic results, possibly related to changes in the activation of anxiety-mediating circuitries and to increases in ammonia plasma levels.Academia Brasileira de Ciências2015-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100331Anais da Academia Brasileira de Ciências v.87 n.1 2015reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201420130262info:eu-repo/semantics/openAccessSUEUR-MALUF,LUCIANA LEVIANA,MILENA B.NAGAOKA,MÁRCIA R.AMORIM,ANA LAURA B.CARDOSO,AMANDA N.RODRIGUES,BRUNA C.MENDES,NATÁLIA F.BITTENCOURT,JACKSON C.CÉSPEDES,ISABEL C.eng2015-11-16T00:00:00Zoai:scielo:S0001-37652015000100331Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2015-11-16T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false
dc.title.none.fl_str_mv Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats
title Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats
spellingShingle Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats
SUEUR-MALUF,LUCIANA LE
anxiety
bile-duct ligation
Fos-immunoreactivity
hepatic encephalopathy
locomotor activity
title_short Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats
title_full Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats
title_fullStr Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats
title_full_unstemmed Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats
title_sort Behavioral alterations and Fos protein immunoreactivity in brain regions of bile duct-ligated cirrhotic rats
author SUEUR-MALUF,LUCIANA LE
author_facet SUEUR-MALUF,LUCIANA LE
VIANA,MILENA B.
NAGAOKA,MÁRCIA R.
AMORIM,ANA LAURA B.
CARDOSO,AMANDA N.
RODRIGUES,BRUNA C.
MENDES,NATÁLIA F.
BITTENCOURT,JACKSON C.
CÉSPEDES,ISABEL C.
author_role author
author2 VIANA,MILENA B.
NAGAOKA,MÁRCIA R.
AMORIM,ANA LAURA B.
CARDOSO,AMANDA N.
RODRIGUES,BRUNA C.
MENDES,NATÁLIA F.
BITTENCOURT,JACKSON C.
CÉSPEDES,ISABEL C.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv SUEUR-MALUF,LUCIANA LE
VIANA,MILENA B.
NAGAOKA,MÁRCIA R.
AMORIM,ANA LAURA B.
CARDOSO,AMANDA N.
RODRIGUES,BRUNA C.
MENDES,NATÁLIA F.
BITTENCOURT,JACKSON C.
CÉSPEDES,ISABEL C.
dc.subject.por.fl_str_mv anxiety
bile-duct ligation
Fos-immunoreactivity
hepatic encephalopathy
locomotor activity
topic anxiety
bile-duct ligation
Fos-immunoreactivity
hepatic encephalopathy
locomotor activity
description Hepatic encephalopathy (HE) encompasses a variety of neuropsychiatric symptoms, including anxiety and psychomotor dysfunction. Although HE is a frequent complication of liver cirrhosis, the neurobiological substrates responsible for its clinical manifestations are largely unclear. In the present study, male Wistar rats were bile duct-ligated (BDL), a procedure which induces liver cirrhosis, and on the 21st day after surgery tested in the elevated plus-maze (EPM) and in an open field for anxiety and locomotor activity measurements. Analysis of Fos protein immunoreactivity (Fos-ir) was used to better understand the neurobiological alterations present in BDL animals. Plasma levels of ammonia were quantified and histopathological analysis of the livers was performed. BDL rats showed a significant decrease in the percentage of entries and time spent in the open arms of the EPM, an anxiogenic effect. These animals also presented significant decreases in Fos-ir in the lateral septal nucleus and medial amygdalar nucleus. Their ammonia plasma levels were significantly higher when compared to the sham group and the diagnosis of cirrhosis was confirmed by histopathological analysis. These results indicate that the BDL model induces anxiogenic results, possibly related to changes in the activation of anxiety-mediating circuitries and to increases in ammonia plasma levels.
publishDate 2015
dc.date.none.fl_str_mv 2015-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100331
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100331
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0001-3765201420130262
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências v.87 n.1 2015
reponame:Anais da Academia Brasileira de Ciências (Online)
instname:Academia Brasileira de Ciências (ABC)
instacron:ABC
instname_str Academia Brasileira de Ciências (ABC)
instacron_str ABC
institution ABC
reponame_str Anais da Academia Brasileira de Ciências (Online)
collection Anais da Academia Brasileira de Ciências (Online)
repository.name.fl_str_mv Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)
repository.mail.fl_str_mv ||aabc@abc.org.br
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