A synthetic peptide selectively kills only virulent Paracoccidioides brasiliensis yeasts
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/33484 http://dx.doi.org/10.1016/j.micinf.2010.10.019 |
Resumo: | This work was conducted to identify virulence biomarkers for Paracoccidioides brasiliensis (Pb), the fungus responsible for Paracoccidioidomycosis (PCM), a systemic disease endemic in Latin America. Measurement of mortality showed that all B10.A mice were killed after 250 days by the virulent Pb18 isolate while only one of the mice that received the attenuated counterpart died. Also, number of lung CFUs from virulent Pb18 inoculated mice were much higher when these isolates were compared. Phage display methodology allowed selection of three phages that specifically bound to virulent Pb18. Variability of p04 phage binding to different Pb isolates were examples of variability of expression by the fungus of its binding molecule, strongly suggesting p04 as a biomarker of virulence. in vitro, its derived peptide pep04 killed only virulent fungi, and confocal microscopy showed that it was internalized only by the virulent isolate. Pep04 blocked establishment of Pb infection in mice and virulent Pb18 pre-incubated with p04 showed significantly inhibited lung infection. Furthermore, infected mice treated with p04 showed highly significant reduction in lung CFUs. These findings firmly establish p04 as a biomarker of Pb virulence. Therefore, after proper peptide engineering, p04 may become a useful adjuvant for the distressing treatment of PCM. (C) 2010 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. |
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Kioshima, Érika Seki [UNIFESP]Aliperti, Fabiana [UNIFESP]Maricato, Juliana Terzi [UNIFESP]Mortara, Renato Arruda [UNIFESP]Bagagli, EduardoMariano, Mario [UNIFESP]Lopes, Jose Daniel [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Univ Estadual Paulista2016-01-24T14:06:13Z2016-01-24T14:06:13Z2011-03-01Microbes and Infection. Amsterdam: Elsevier B.V., v. 13, n. 3, p. 251-260, 2011.1286-4579http://repositorio.unifesp.br/handle/11600/33484http://dx.doi.org/10.1016/j.micinf.2010.10.019WOS000287615000006.pdf10.1016/j.micinf.2010.10.019WOS:000287615000006This work was conducted to identify virulence biomarkers for Paracoccidioides brasiliensis (Pb), the fungus responsible for Paracoccidioidomycosis (PCM), a systemic disease endemic in Latin America. Measurement of mortality showed that all B10.A mice were killed after 250 days by the virulent Pb18 isolate while only one of the mice that received the attenuated counterpart died. Also, number of lung CFUs from virulent Pb18 inoculated mice were much higher when these isolates were compared. Phage display methodology allowed selection of three phages that specifically bound to virulent Pb18. Variability of p04 phage binding to different Pb isolates were examples of variability of expression by the fungus of its binding molecule, strongly suggesting p04 as a biomarker of virulence. in vitro, its derived peptide pep04 killed only virulent fungi, and confocal microscopy showed that it was internalized only by the virulent isolate. Pep04 blocked establishment of Pb infection in mice and virulent Pb18 pre-incubated with p04 showed significantly inhibited lung infection. Furthermore, infected mice treated with p04 showed highly significant reduction in lung CFUs. These findings firmly establish p04 as a biomarker of Pb virulence. Therefore, after proper peptide engineering, p04 may become a useful adjuvant for the distressing treatment of PCM. (C) 2010 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, BrazilState Univ São Paulo, Dept Microbiol, Inst Biosci Botucatu, Botucatu, SP, BrazilUniv Estadual Paulista, Inst Hlth, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, BrazilFAPESP: 04/04471-9Web of Science251-260engElsevier B.V.Microbes and Infectionhttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyinfo:eu-repo/semantics/openAccessP. brasiliensisVirulenceBiomarkersA synthetic peptide selectively kills only virulent Paracoccidioides brasiliensis yeastsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000287615000006.pdfapplication/pdf872274${dspace.ui.url}/bitstream/11600/33484/1/WOS000287615000006.pdf4c5a188b50b54b66fda235cd33f3100fMD51open accessTEXTWOS000287615000006.pdf.txtWOS000287615000006.pdf.txtExtracted texttext/plain46653${dspace.ui.url}/bitstream/11600/33484/2/WOS000287615000006.pdf.txtcdc843296ab3ed15adf234e89e54560fMD52open access11600/334842022-02-18 10:14:11.513open accessoai:repositorio.unifesp.br:11600/33484Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:08:05.529419Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
A synthetic peptide selectively kills only virulent Paracoccidioides brasiliensis yeasts |
title |
A synthetic peptide selectively kills only virulent Paracoccidioides brasiliensis yeasts |
spellingShingle |
A synthetic peptide selectively kills only virulent Paracoccidioides brasiliensis yeasts Kioshima, Érika Seki [UNIFESP] P. brasiliensis Virulence Biomarkers |
title_short |
A synthetic peptide selectively kills only virulent Paracoccidioides brasiliensis yeasts |
title_full |
A synthetic peptide selectively kills only virulent Paracoccidioides brasiliensis yeasts |
title_fullStr |
A synthetic peptide selectively kills only virulent Paracoccidioides brasiliensis yeasts |
title_full_unstemmed |
A synthetic peptide selectively kills only virulent Paracoccidioides brasiliensis yeasts |
title_sort |
A synthetic peptide selectively kills only virulent Paracoccidioides brasiliensis yeasts |
author |
Kioshima, Érika Seki [UNIFESP] |
author_facet |
Kioshima, Érika Seki [UNIFESP] Aliperti, Fabiana [UNIFESP] Maricato, Juliana Terzi [UNIFESP] Mortara, Renato Arruda [UNIFESP] Bagagli, Eduardo Mariano, Mario [UNIFESP] Lopes, Jose Daniel [UNIFESP] |
author_role |
author |
author2 |
Aliperti, Fabiana [UNIFESP] Maricato, Juliana Terzi [UNIFESP] Mortara, Renato Arruda [UNIFESP] Bagagli, Eduardo Mariano, Mario [UNIFESP] Lopes, Jose Daniel [UNIFESP] |
author2_role |
author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade de São Paulo (USP) Univ Estadual Paulista |
dc.contributor.author.fl_str_mv |
Kioshima, Érika Seki [UNIFESP] Aliperti, Fabiana [UNIFESP] Maricato, Juliana Terzi [UNIFESP] Mortara, Renato Arruda [UNIFESP] Bagagli, Eduardo Mariano, Mario [UNIFESP] Lopes, Jose Daniel [UNIFESP] |
dc.subject.eng.fl_str_mv |
P. brasiliensis Virulence Biomarkers |
topic |
P. brasiliensis Virulence Biomarkers |
description |
This work was conducted to identify virulence biomarkers for Paracoccidioides brasiliensis (Pb), the fungus responsible for Paracoccidioidomycosis (PCM), a systemic disease endemic in Latin America. Measurement of mortality showed that all B10.A mice were killed after 250 days by the virulent Pb18 isolate while only one of the mice that received the attenuated counterpart died. Also, number of lung CFUs from virulent Pb18 inoculated mice were much higher when these isolates were compared. Phage display methodology allowed selection of three phages that specifically bound to virulent Pb18. Variability of p04 phage binding to different Pb isolates were examples of variability of expression by the fungus of its binding molecule, strongly suggesting p04 as a biomarker of virulence. in vitro, its derived peptide pep04 killed only virulent fungi, and confocal microscopy showed that it was internalized only by the virulent isolate. Pep04 blocked establishment of Pb infection in mice and virulent Pb18 pre-incubated with p04 showed significantly inhibited lung infection. Furthermore, infected mice treated with p04 showed highly significant reduction in lung CFUs. These findings firmly establish p04 as a biomarker of Pb virulence. Therefore, after proper peptide engineering, p04 may become a useful adjuvant for the distressing treatment of PCM. (C) 2010 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-03-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:06:13Z |
dc.date.available.fl_str_mv |
2016-01-24T14:06:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Microbes and Infection. Amsterdam: Elsevier B.V., v. 13, n. 3, p. 251-260, 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/33484 http://dx.doi.org/10.1016/j.micinf.2010.10.019 |
dc.identifier.issn.none.fl_str_mv |
1286-4579 |
dc.identifier.file.none.fl_str_mv |
WOS000287615000006.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.micinf.2010.10.019 |
dc.identifier.wos.none.fl_str_mv |
WOS:000287615000006 |
identifier_str_mv |
Microbes and Infection. Amsterdam: Elsevier B.V., v. 13, n. 3, p. 251-260, 2011. 1286-4579 WOS000287615000006.pdf 10.1016/j.micinf.2010.10.019 WOS:000287615000006 |
url |
http://repositorio.unifesp.br/handle/11600/33484 http://dx.doi.org/10.1016/j.micinf.2010.10.019 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Microbes and Infection |
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http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy info:eu-repo/semantics/openAccess |
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http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
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openAccess |
dc.format.none.fl_str_mv |
251-260 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
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Elsevier B.V. |
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