The effect of n-acetylcysteine on hepatic histomorphology during hypothermic preservation
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014001500028 |
Resumo: | PURPOSE: To evaluate the NAC effects on liver hypothermic preservation at different time intervals. METHODS: For this, we used livers of male Wistar rats weighing between 250 and 300g, undergoing preservation in Ringer solution at 4°C for up to 24 hours. Tissue samples were obtained at four moments of preservation for histological examination by hematoxylin and eosin staining: T0 = beginning of preservation, T12 = 12 hours, T18 = 18 hours and T24 = 24 hours. Will be analyzed vacuolation, hepatic apoptosis by optical microscopy and parenchymal. RESULTS: The results showed a progressive increase in hepatic injury in both groups and showed that NAC was effective at T0. The parenchyma preservation was better in the NAC group and no difference when vacuolization of the cells. CONCLUSION: Hypothermic preservation, over time, causes changes in the hepatic parenchyma with increased apoptosis, loss of architecture, vacuolization, culminating in severe injury. The administration of N-acetylcysteine protects against preservation liver injury. |
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Acta Cirúrgica Brasileira (Online) |
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The effect of n-acetylcysteine on hepatic histomorphology during hypothermic preservationAcetylcysteineLiverHypothermiaOrgan PreservationRats PURPOSE: To evaluate the NAC effects on liver hypothermic preservation at different time intervals. METHODS: For this, we used livers of male Wistar rats weighing between 250 and 300g, undergoing preservation in Ringer solution at 4°C for up to 24 hours. Tissue samples were obtained at four moments of preservation for histological examination by hematoxylin and eosin staining: T0 = beginning of preservation, T12 = 12 hours, T18 = 18 hours and T24 = 24 hours. Will be analyzed vacuolation, hepatic apoptosis by optical microscopy and parenchymal. RESULTS: The results showed a progressive increase in hepatic injury in both groups and showed that NAC was effective at T0. The parenchyma preservation was better in the NAC group and no difference when vacuolization of the cells. CONCLUSION: Hypothermic preservation, over time, causes changes in the hepatic parenchyma with increased apoptosis, loss of architecture, vacuolization, culminating in severe injury. The administration of N-acetylcysteine protects against preservation liver injury. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014001500028Acta Cirúrgica Brasileira v.29 suppl.3 2014reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502014001700006info:eu-repo/semantics/openAccessRisso,Patricia SayuriKoike,Marcia KiyomiAbrahão,Marcos de SouzaFerreira,Natalie ChavesMontero,Edna Frasson de Souzaeng2015-07-30T00:00:00Zoai:scielo:S0102-86502014001500028Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2015-07-30T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
The effect of n-acetylcysteine on hepatic histomorphology during hypothermic preservation |
title |
The effect of n-acetylcysteine on hepatic histomorphology during hypothermic preservation |
spellingShingle |
The effect of n-acetylcysteine on hepatic histomorphology during hypothermic preservation Risso,Patricia Sayuri Acetylcysteine Liver Hypothermia Organ Preservation Rats |
title_short |
The effect of n-acetylcysteine on hepatic histomorphology during hypothermic preservation |
title_full |
The effect of n-acetylcysteine on hepatic histomorphology during hypothermic preservation |
title_fullStr |
The effect of n-acetylcysteine on hepatic histomorphology during hypothermic preservation |
title_full_unstemmed |
The effect of n-acetylcysteine on hepatic histomorphology during hypothermic preservation |
title_sort |
The effect of n-acetylcysteine on hepatic histomorphology during hypothermic preservation |
author |
Risso,Patricia Sayuri |
author_facet |
Risso,Patricia Sayuri Koike,Marcia Kiyomi Abrahão,Marcos de Souza Ferreira,Natalie Chaves Montero,Edna Frasson de Souza |
author_role |
author |
author2 |
Koike,Marcia Kiyomi Abrahão,Marcos de Souza Ferreira,Natalie Chaves Montero,Edna Frasson de Souza |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Risso,Patricia Sayuri Koike,Marcia Kiyomi Abrahão,Marcos de Souza Ferreira,Natalie Chaves Montero,Edna Frasson de Souza |
dc.subject.por.fl_str_mv |
Acetylcysteine Liver Hypothermia Organ Preservation Rats |
topic |
Acetylcysteine Liver Hypothermia Organ Preservation Rats |
description |
PURPOSE: To evaluate the NAC effects on liver hypothermic preservation at different time intervals. METHODS: For this, we used livers of male Wistar rats weighing between 250 and 300g, undergoing preservation in Ringer solution at 4°C for up to 24 hours. Tissue samples were obtained at four moments of preservation for histological examination by hematoxylin and eosin staining: T0 = beginning of preservation, T12 = 12 hours, T18 = 18 hours and T24 = 24 hours. Will be analyzed vacuolation, hepatic apoptosis by optical microscopy and parenchymal. RESULTS: The results showed a progressive increase in hepatic injury in both groups and showed that NAC was effective at T0. The parenchyma preservation was better in the NAC group and no difference when vacuolization of the cells. CONCLUSION: Hypothermic preservation, over time, causes changes in the hepatic parenchyma with increased apoptosis, loss of architecture, vacuolization, culminating in severe injury. The administration of N-acetylcysteine protects against preservation liver injury. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014001500028 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014001500028 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-86502014001700006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.29 suppl.3 2014 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126442788880384 |