Technical challenges of working with extracellular vesicles
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| Texto Completo: | https://repositorio.unifesp.br/handle/11600/54256 https://dx.doi.org/10.1039/c7nr08360b |
Resumo: | Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nano-sized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review. |
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Ramirez, Marcel IvanAmorim, Maria G.Gadelha, CatarinaMilic, IvanaWelsh, Joshua A.Freitas, Vanessa MoraisNawaz, MuhammadAkbar, NaveedCouch, YvonneMakin, LauraCooke, FionaVettore, Andre Luiz [UNIFESP]Xander, Patricia [UNIFESP]Freezor, RobertaPezuk, Julia A.Rosa-Fernandes, LiviaCarreira, Ana Claudia OliveiraDevitt, AndrewJacobs, LauraSilva, Israel T.Coakley, GillianNunes, Diana NoronhaCarter, DavePalmisano, GiuseppeDias-Neto, Emmanuel2020-07-08T13:09:52Z2020-07-08T13:09:52Z2018Nanoscale. Cambridge, v. 10, n. 3, p. 881-906, 2018.2040-3364https://repositorio.unifesp.br/handle/11600/54256https://dx.doi.org/10.1039/c7nr08360bWOS000423259000001.pdf10.1039/c7nr08360bWOS:000423259000001Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nano-sized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.British Council/Newton Fund, BrazilAssociação Beneficente Alzira Denise Hertzog Silva (ABADHS), BrazilRicerca Finalizzata from the Ministero della Salute, ItalyScience without Borders/CAPES, BrazilFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundacao Oswaldo Cruz, Rio De Janeiro, RJ, BrazilUniv Fed Parana, Curitiba, Parana, BrazilAC Camargo Canc Ctr, CIPE, Sao Paulo, SP, BrazilUniv Nottingham, Sch Life Sci, Nottingham, EnglandAston Univ, Sch Life & Hlth Sci, Birmingham, W Midlands, EnglandUniv Southampton, Southampton, EnglandUniv Sao Paulo, Sao Paulo, SP, BrazilUniv Gothenburg, Gothenburg, SwedenUniv Oxford, Div Cardiovasc Med, Oxford, EnglandUniv Oxford, Acute Stroke Programme, RDM Investigat Med, Oxford, EnglandUniv Oxford, Sir William Dunn Sch Pathol, Oxford, EnglandUniv St Andrews, St Andrews, Fife, ScotlandUniv Fed Sao Paulo, Campus Diadema, Diadema, BrazilLondon Metropolitan Univ, London, EnglandUniv Anhanguera Sao Paulo, Sao Paulo, BrazilUniv Southern Denmark, Odense, DenmarkTech Univ Munich, Munich, GermanyUniv Glasgow, Inst Infect Immun & Inflammat, Glasgow, Lanark, ScotlandOxford Brookes Univ, Oxford, EnglandFdn Santa Lucia, IRCCS, Rome, ItalyUniv Fed Sao Paulo, Campus Diadema, Diadema, BrazilBritish Council/Newton Fund, Brazil:203855854FAPESP: 2012/24574-3Science without Borders/CAPES, Brazil: BEX 7057/15-6FAPESP: 2015/50257-3FAPESP: 2014/26897-0FAPESP: 2014/06863-3CNPq: 441878/2014-8CNPq: 202077/2015-2Ricerca Finalizzata from the Ministero della Salute, Italy: 172/GR-2011-02350301Web of Science881-906engRoyal Soc ChemistryNanoscaleTechnical challenges of working with extracellular vesiclesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCambridge103info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000423259000001.pdfapplication/pdf1287687${dspace.ui.url}/bitstream/11600/54256/1/WOS000423259000001.pdf2a9b6d8f6cedc28eaaba619634328a09MD51open accessTEXTWOS000423259000001.pdf.txtWOS000423259000001.pdf.txtExtracted texttext/plain151291${dspace.ui.url}/bitstream/11600/54256/8/WOS000423259000001.pdf.txta79122207f82aa68ef92cba953aac59fMD58open accessTHUMBNAILWOS000423259000001.pdf.jpgWOS000423259000001.pdf.jpgIM Thumbnailimage/jpeg8114${dspace.ui.url}/bitstream/11600/54256/10/WOS000423259000001.pdf.jpg2cfe0a0e1e8855c87e2ffdd000d07325MD510open access11600/542562023-06-05 19:25:57.815open accessoai:repositorio.unifesp.br:11600/54256Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:25:57Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.en.fl_str_mv |
Technical challenges of working with extracellular vesicles |
| title |
Technical challenges of working with extracellular vesicles |
| spellingShingle |
Technical challenges of working with extracellular vesicles Ramirez, Marcel Ivan |
| title_short |
Technical challenges of working with extracellular vesicles |
| title_full |
Technical challenges of working with extracellular vesicles |
| title_fullStr |
Technical challenges of working with extracellular vesicles |
| title_full_unstemmed |
Technical challenges of working with extracellular vesicles |
| title_sort |
Technical challenges of working with extracellular vesicles |
| author |
Ramirez, Marcel Ivan |
| author_facet |
Ramirez, Marcel Ivan Amorim, Maria G. Gadelha, Catarina Milic, Ivana Welsh, Joshua A. Freitas, Vanessa Morais Nawaz, Muhammad Akbar, Naveed Couch, Yvonne Makin, Laura Cooke, Fiona Vettore, Andre Luiz [UNIFESP] Xander, Patricia [UNIFESP] Freezor, Roberta Pezuk, Julia A. Rosa-Fernandes, Livia Carreira, Ana Claudia Oliveira Devitt, Andrew Jacobs, Laura Silva, Israel T. Coakley, Gillian Nunes, Diana Noronha Carter, Dave Palmisano, Giuseppe Dias-Neto, Emmanuel |
| author_role |
author |
| author2 |
Amorim, Maria G. Gadelha, Catarina Milic, Ivana Welsh, Joshua A. Freitas, Vanessa Morais Nawaz, Muhammad Akbar, Naveed Couch, Yvonne Makin, Laura Cooke, Fiona Vettore, Andre Luiz [UNIFESP] Xander, Patricia [UNIFESP] Freezor, Roberta Pezuk, Julia A. Rosa-Fernandes, Livia Carreira, Ana Claudia Oliveira Devitt, Andrew Jacobs, Laura Silva, Israel T. Coakley, Gillian Nunes, Diana Noronha Carter, Dave Palmisano, Giuseppe Dias-Neto, Emmanuel |
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author author author author author author author author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Ramirez, Marcel Ivan Amorim, Maria G. Gadelha, Catarina Milic, Ivana Welsh, Joshua A. Freitas, Vanessa Morais Nawaz, Muhammad Akbar, Naveed Couch, Yvonne Makin, Laura Cooke, Fiona Vettore, Andre Luiz [UNIFESP] Xander, Patricia [UNIFESP] Freezor, Roberta Pezuk, Julia A. Rosa-Fernandes, Livia Carreira, Ana Claudia Oliveira Devitt, Andrew Jacobs, Laura Silva, Israel T. Coakley, Gillian Nunes, Diana Noronha Carter, Dave Palmisano, Giuseppe Dias-Neto, Emmanuel |
| description |
Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nano-sized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review. |
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2018 |
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2018 |
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2020-07-08T13:09:52Z |
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2020-07-08T13:09:52Z |
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info:eu-repo/semantics/publishedVersion |
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Nanoscale. Cambridge, v. 10, n. 3, p. 881-906, 2018. |
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https://repositorio.unifesp.br/handle/11600/54256 https://dx.doi.org/10.1039/c7nr08360b |
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2040-3364 |
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WOS000423259000001.pdf |
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10.1039/c7nr08360b |
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Nanoscale. Cambridge, v. 10, n. 3, p. 881-906, 2018. 2040-3364 WOS000423259000001.pdf 10.1039/c7nr08360b WOS:000423259000001 |
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https://repositorio.unifesp.br/handle/11600/54256 https://dx.doi.org/10.1039/c7nr08360b |
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