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Efeito da suplementação com benfotiamina sobre parâmetros metabólicos e desempenho de endurance de camundongos submetidos a treinamento de nataçãoExercícios físicos.Tiamina.Estresse Oxidativo.Suplementos Nutricionais.Resistência física.Physical exercise.Thiamine.Oxidative stress.Nutritional supplementation.Endurance exercise.Metabolismo e BioenergéticaIntrodução: Exercícios físicos aumentam a demanda energética, fazendo-se necessário o adequado consumo de nutrientes a fim de aperfeiçoar a produção de energia, possibilitar a melhor recuperação e otimizar os processos adaptativos. O difosfato de tiamina (TDP) é cofator essencial para o funcionamento das enzimas complexo piruvato-desidrogenase (PDH), α- cetoglutarato desidrogenase (OGDH) e transcetolase (TKT), fundamentais no metabolismo energético. A benfotiamina é um análogo sintético da tiamina, capaz de promover uma biodisponibilidade de TDP maior em comparação a outros sais de tiamina. Objetivo: O objetivo deste estudo foi avaliar os efeitos da suplementação com benfotiamina sobre os parâmetros metabólicos e de desempenho em camundongos submetidos o treinamento físico de endurance. Métodos: A pesquisa utilizou 25 camundongos BALB/c, machos com seis semanas de idade. Os animais foram separados em 4 grupos: Dieta padrão e sedentarismo (Pad-Sed); Dieta padrão com treinamento em natação (Pad-Tr); Dieta suplementada com benfotiamina e sedentário (Ben-Sed); Dieta suplementada com benfotiamina e treinamento em natação (Ben-Tr). A benfotiamina foi adicionada à ração AIN-93 (500mg/kg). Os animais foram submetidos a treinamento de natação durante 6 semanas. O teste de exaustão foi a última sessão de natação com sobrecarga de 5% da massa corporal. A concentração plasmática de lactato foi dosada antes e imediatamente após o teste de exaustão. As concentrações de substâncias reativas ao ácido tiobarbitúrico (TBARS), proteínas carboniladas, tióis totais e tióis não-proteicos foram analisadas no fígado, coração e músculo tibial anterior. As concentrações de tiamina nos eritrócitos e no músculo gastrocnêmio foram avaliadas por cromatografia líquida de alta eficiência. A expressão do RNAm dos genes PDHa1 e OGDH no músculo gastrocnêmio foi avaliada por Transcrição reversa-reação em cadeia da polimerase em tempo real (qRT-PCR). A concentração dos ácidos pirúvico, lático e hidroxibutírico foram quantificados no músculo por cromatografia gasosa acoplada ao espectrômetro de massas (GC-MS). Resultados: Os animais suplementados apresentaram níveis mais elevados de tiamina livre, monofosfato de tiamina e difosfato de tiamina nos eritrócitos e nos músculos. No músculo tibial, a peroxidação lipídica foi maior no grupo Pad-Sed foi maior, enquanto no coração, a peroxidação lipídica nos grupos Pad-Sed e Ben-Tr foi maior do que no grupo Ben-Sed. A concentração de proteínas carboniladas no músculo foi maior no grupo Pad-Sed do que emambos os grupos suplementados. No fígado, a carbonilação de proteínas foi menor no grupo Ben-Sed do que no Pad-Sed. O nível de tióis totais foi menor no grupo Ben-Sed do que no PadTr. No coração, o nível de tióis totais foi mais alto no grupo Ben-Sed do que no Ben-Tr. A concentração de tióis não-proteicos no músculo foi maior no grupo Ben-Sed do que no Ben-Tr, enquanto no coração, a concentração de tióis não-proteicos do grupo Pad-Tr foi menor do que no grupo Pad-Sed. Não houve diferença na expressão gênica entre todos os grupos. O músculo dos animais treinados suplementados apresentou maiores concentrações de ácido lático e de ácido hidroxibutírico que animais sedentários. A razão ácido lático:ácido pirúvico foi maior nos animais treinados. Não houve diferença na capacidade de endurance entre os grupos Pad-Tr e Ben-Tr. Da mesma forma, a concentração final de lactato também não foi diferente entre os grupos. Conclusão: A suplementação oral com benfotiamina aumenta a concentração de tiamina e seus éteres nos eritrócitos e no músculo gastrocnêmio. A benfotiamina se mostrou um antioxidante eficiente contra o estresse oxidativo no músculo tibial anterior e no coração de animais submetidos ao treinamento de endurance. Entretanto, não é capaz de afetar a expressão dos genes de enzimas dependente de tiamina no músculo gastrocnêmio. A suplementação aumentou o catabolismo do piruvato no músculo dos animais treinados, mas não apresentou efeito antifadiga.Introduction: Physical exercises increase energy demand, making it necessary an adequate nutrients intake to improve energy production, aiming to promote better recovery and optimize adaptive processes. Thiamine diphosphate (TDP) is an essential cofactor for the functioning of the enzymes pyruvate dehydrogenase (PDH), α-ketoglutarate dehydrogenase (OGDH), and transketolase (TKT), which are fundamentals in energy metabolism. Benfotiamine is a synthetic analogue of thiamine capable of promoting a greater bioavailability of TDP compared to other thiamine salts. Objective: The objective of this study was to evaluate the effects of supplementation with benfotiamine on metabolic and performance parameters in mice, physical training of endurance. Methods: Twenty-five male BALB/c mice were allocated to the following treatment groups: standard diet and sedentary activity (Sta-Sed), benfotiamine–supplemented diet and sedentary activity (Ben-Sed), standard diet and training activity (Sta-Tr) and benfotiamine–supplemented diet and training activity (Ben-Tr). Benfotiamine was added to the AIN-93 diet (500mg/Kg). The training comprised 6 weeks of endurance swimming training. The exhaustion test was the last swimming session with an overload of 5% of the body weight attached to the tail. The plasma lactate concentration was measured before and immediately after the exhaustion test. The concentration of thiobarbituric acid reactive substances (TBARS), carbonylated proteins, total thiols and non-protein thiols was analyzed in the liver, heart and tibialis anterior muscle. The thiamine concentration in erythrocytes and in gastrocnemius muscle was assessed using high performance liquid chromatography. The expression of the genes PDHa1 and OGDH in gastrocnemius muscle was evaluated by reverse transcriptase-Reaction in the polymerase chain in real time (qRT-PCR). Pyruvic, lactic and hydroxybutyric acids were quantified in muscle by gas chromatography coupled to the mass spectrometer (GC-MS). Results: Supplemented animals showed higher levels of thiamine, thiamine monophosphate and thiamine diphosphate in the erythrocytes and in the muscle. In the tibialis muscle, lipid peroxidation was higher in the Sta-Sed group was higher while in the heart, lipid peroxidation in the Sta-Sed and Ben-Tr groups was higher than in the Ben-Sed group. The carbonyl content in the muscle was higher in the Sta-Sed group than in both supplemented groups. In liver, the carbonyl content was lower in the Ben-Sed group than in the Sta-Sed. The level of total thiols was lower in the Ben-Sed group than in the Sta-Tr. In the heart, the level of total thiols washigher in the Ben-Sed group than in the Ben-Tr. The concentration of non-protein thiols in the muscle was higher in the Ben-Sed group than in the Ben-Tr, whereas in the heart, concentration of non-protein thiols of Sta-Tr group was lower than in the Sta-Sed. There was no difference in gene expression between all groups. The muscle of the Ben-Tr animals showed higher concentrations of lactic acid and hydroxybutyric acid than sedentary animals. The lactic acid: pyruvic acid ratio was higher in the trained animals. There was no difference in endurance capacity between the Pad-Tr and Ben-Tr groups. Similarly, the final lactate concentration was also no different between groups. Conclusion: Oral supplementation with benfotiamine increases the concentration of thiamine and its ethers in the erythrocytes and in the gastrocnemius muscle. It is an efficient antioxidant against oxidative stress in the anterior tibial muscle and in the heart of animals submitted to endurance training. However, it is not able to affect the expression of thiamine-dependent enzyme genes in gastrocnemius muscle. The supplementation increased the pyruvate catabolism in muscle of trained mice, but did not affect endurance performance.Universidade Federal do Triângulo MineiroInstituto de Ciências da Saúde - ICS::Programa de Pós-Graduação em Ciências da SaúdeBrasilUFTMPrograma de Pós-Graduação em Ciências da SaúdePORTARI, Guilherme Vannucchi26157082879http://lattes.cnpq.br/6076945534196087GONÇALVES, Alisson de Carvalho2021-11-25T16:00:52Z2021-05-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfapplication/pdfGONÇALVES, Alisson de Carvalho. Efeito da suplementação com benfotiamina sobre parâmetros metabólicos e desempenho de endurance de camundongos submetidos a treinamento de natação. 2021. 123f. Tese (Doutorado em Ciências da Saúde) - Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal do Triângulo Mineiro, Uberaba, 2021.http://bdtd.uftm.edu.br/handle/tede/1054porACÍN-PÉREZ, Rebeca et al. 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Postgraduate Medicine Journal, v. 73, p. 27-31, 1997.http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFTMinstname:Universidade Federal do Triangulo Mineiro (UFTM)instacron:UFTM2021-11-26T04:00:10Zoai:bdtd.uftm.edu.br:tede/1054Biblioteca Digital de Teses e Dissertaçõeshttp://bdtd.uftm.edu.br/PUBhttp://bdtd.uftm.edu.br/oai/requestbdtd@uftm.edu.br||bdtd@uftm.edu.bropendoar:2024-04-24T09:59:50.139191Biblioteca Digital de Teses e Dissertações da UFTM - Universidade Federal do Triangulo Mineiro (UFTM)false
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