Efeito da suplementação com benfotiamina sobre parâmetros de estresse oxidativo muscular de camundongos submetidos a treinamento de endurance

Detalhes bibliográficos
Autor(a) principal: GONÇALVES, Álisson de Carvalho
Data de Publicação: 2017
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFTM
Texto Completo: http://bdtd.uftm.edu.br/handle/tede/367
Resumo: Os altos níveis de espécies reativas de oxigênio podem desencadear um desequilíbrio no estado redox capaz de gerar danos oxidativos a macromoléculas. O presente estudo objetivou investigar a influência da suplementação oral com benfotiamina sobre o estresse oxidativo e a atividade antioxidante no músculo de camundongos treinados. Vinte e cinco ratos Balb / c machos foram alocados nos grupos Pad-Sed: dieta padrão e sedentário (n=6); Ben-Sed: suplementação com benfotiamina e sedentário (n=6); Sta-Tr: dieta padrão e treinado (n=6); Ben-Tr: Suplementação com benfotiamina e treinado (n=7). Os camundongos treinados foram submetidos a treinamento de endurance em natação durante 6 semanas, com um teste de exaustão ao final do protocolo de treinamento. A concentração de malondialdeído livre (MDA), proteínas ligadas a malondialdeído (PrMDA) e tióis não proteicos bem como a atividade das enzimas catalase (CAT) e superóxido dismutase (SOD) foram analisadas no músculo gastrocnêmio. Não houve diferença no tempo de resistência à exaustão e na concentração de lactato pós-exaustão. A concentração de MDA foi menor no grupo Ben-Tr em relação aos grupos sedentários. A concentração de PrMDA foi maior no grupo Pad-Sed em relação aos demais grupos. Os nívéis de tióis foram maiores no grupo Ben-Sed em relação aos grupos não suplementados. A atividade da CAT foi mais acentuada em ambos os grupos suplementados. A SOD mostrou-se mais eficiente no grupo Ben-Tr que nos grupos não suplementados. Os resultados mostraram que a suplementação de benfotiamina é uma estratégia antioxidante eficiente, capaz de prevenir os danos oxidativos musculares em indivíduos engajados em programas de treinamento em endurance. Em conclusão, a benfotiamina é eficiente em prevenir danos oxidativos musculares e não interfere no desempenho de endurance.
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spelling Efeito da suplementação com benfotiamina sobre parâmetros de estresse oxidativo muscular de camundongos submetidos a treinamento de enduranceTiamina.Estresse Oxidativo.Esforço FísicoOxidative stress.Physical effort.Thiamine.Metabolismo e BioenergéticaOs altos níveis de espécies reativas de oxigênio podem desencadear um desequilíbrio no estado redox capaz de gerar danos oxidativos a macromoléculas. O presente estudo objetivou investigar a influência da suplementação oral com benfotiamina sobre o estresse oxidativo e a atividade antioxidante no músculo de camundongos treinados. Vinte e cinco ratos Balb / c machos foram alocados nos grupos Pad-Sed: dieta padrão e sedentário (n=6); Ben-Sed: suplementação com benfotiamina e sedentário (n=6); Sta-Tr: dieta padrão e treinado (n=6); Ben-Tr: Suplementação com benfotiamina e treinado (n=7). Os camundongos treinados foram submetidos a treinamento de endurance em natação durante 6 semanas, com um teste de exaustão ao final do protocolo de treinamento. A concentração de malondialdeído livre (MDA), proteínas ligadas a malondialdeído (PrMDA) e tióis não proteicos bem como a atividade das enzimas catalase (CAT) e superóxido dismutase (SOD) foram analisadas no músculo gastrocnêmio. Não houve diferença no tempo de resistência à exaustão e na concentração de lactato pós-exaustão. A concentração de MDA foi menor no grupo Ben-Tr em relação aos grupos sedentários. A concentração de PrMDA foi maior no grupo Pad-Sed em relação aos demais grupos. Os nívéis de tióis foram maiores no grupo Ben-Sed em relação aos grupos não suplementados. A atividade da CAT foi mais acentuada em ambos os grupos suplementados. A SOD mostrou-se mais eficiente no grupo Ben-Tr que nos grupos não suplementados. Os resultados mostraram que a suplementação de benfotiamina é uma estratégia antioxidante eficiente, capaz de prevenir os danos oxidativos musculares em indivíduos engajados em programas de treinamento em endurance. Em conclusão, a benfotiamina é eficiente em prevenir danos oxidativos musculares e não interfere no desempenho de endurance.The high levels of reactive oxygen species can trigger an imbalance in redox status, which generate oxidative damage in macromolecules. The present study aimed investigate the influence of oral supplementation with benfotiamine on oxidative stress and antioxidant activity in muscle of trained mice. Twenty-five male Balb/c mice was placed in groups: Pad-Sed) standard chow and sedentary (n=6); Ben-Sed: benfotiamine supplemented and sedentary (n=6); Pad-Tr: standard chow and trained (n=6); and Ben-Tr: benfotiamine supplemented and trained (n=7). Supplemented animals received chow AIN-93G with benfotiamine. Trained mice was submitted to 6-weeks endurance swimming training, with an exhaustion test at the end. The concentration of free malondialdehyde (MDA), proteins bound to malondialdehyde (PrMDA) and non-protein thiols as well as the activity of catalase (CAT) and superoxide dismutase (SOD) were analyzed in the gastrocnemius muscle. There was no difference in the time of exhaustion resistance and the post-exhaustion lactate concentration. The concentration of MDA was lower in the Ben-Tr group than in the sedentary groups. The concentration of PrMDA was higher in the Pad-Sed group than in the other groups. Thiol levels were higher in the Ben-Sed group than in the non-supplemented groups. CAT activity was more pronounced in both supplemented groups. SOD was more efficient in the Ben-Tr group than in the non-supplemented groups. The results showed that benfotiamine supplementation is an efficient antioxidant strategy capable of preventing muscle oxidative damage in individuals engaged in endurance training programs. In conclusion, benfotiamine is effective in preventing muscle oxidative damage and does not interfere in endurance performance.Universidade Federal do Triângulo MineiroInstituto de Ciências da Saúde - ICS::Curso de Graduação em Educação FísicaBrasilUFTMPrograma de Pós-Graduação em Educação FísicaPORTARI, Guilherme Vannucchi26157082879http://lattes.cnpq.br/6076945534196087GONÇALVES, Álisson de Carvalho2017-04-01T13:49:14Z2017-02-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfGONÇALVES, Álisson de Carvalho. Efeito da suplementação com benfotiamina sobre parâmetros de estresse oxidativo muscular de camundongos submetidos a treinamento de endurance. 2017. 54f. Dissertação (Mestrado em Educação Física) - Programa de Pós-Graduação em Educação Física, Universidade Federal do Triângulo Mineiro, Uberaba, 2017.http://bdtd.uftm.edu.br/handle/tede/367porANDERSON, E. J.; KATUNGA, L. A.; WILLIS, M. S. Mitochondria as a source and target of lipid peroxidation products in healthy and diseased heart. Clinical and Experimental Pharmacology and Physiology, v. 39, n. 2, p. 179-193, 2012. ANDRADE, F. H. et al. Effect of hydrogen peroxide and dithiothreitol on contractile function of single skeletal muscle fibres from the mouse. The Journal of physiology, v. 509, n. 2, p. 565-575, 1998. ANDRADE, F. H.; REID, M. B.; WESTERBLAD, H. Contractile response of skeletal muscle to low peroxide concentrations: myofibrillar calcium sensitivity as a likely target for redoxmodulation. The FASEB Journal, v. 15, n. 2, p. 309-311, 2001. ASLANI, B. A.; GHOBADI, S. Studies on oxidants and antioxidants with a brief glance at their relevance to the immune system. 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Postgraduate Medicine Journal, v. 73, p. 27-31, 1997.http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFTMinstname:Universidade Federal do Triangulo Mineiro (UFTM)instacron:UFTM2018-03-23T18:43:33Zoai:bdtd.uftm.edu.br:tede/367Biblioteca Digital de Teses e Dissertaçõeshttp://bdtd.uftm.edu.br/PUBhttp://bdtd.uftm.edu.br/oai/requestbdtd@uftm.edu.br||bdtd@uftm.edu.bropendoar:2018-03-23T18:43:33Biblioteca Digital de Teses e Dissertações da UFTM - Universidade Federal do Triangulo Mineiro (UFTM)false
dc.title.none.fl_str_mv Efeito da suplementação com benfotiamina sobre parâmetros de estresse oxidativo muscular de camundongos submetidos a treinamento de endurance
title Efeito da suplementação com benfotiamina sobre parâmetros de estresse oxidativo muscular de camundongos submetidos a treinamento de endurance
spellingShingle Efeito da suplementação com benfotiamina sobre parâmetros de estresse oxidativo muscular de camundongos submetidos a treinamento de endurance
GONÇALVES, Álisson de Carvalho
Tiamina.
Estresse Oxidativo.
Esforço Físico
Oxidative stress.
Physical effort.
Thiamine.
Metabolismo e Bioenergética
title_short Efeito da suplementação com benfotiamina sobre parâmetros de estresse oxidativo muscular de camundongos submetidos a treinamento de endurance
title_full Efeito da suplementação com benfotiamina sobre parâmetros de estresse oxidativo muscular de camundongos submetidos a treinamento de endurance
title_fullStr Efeito da suplementação com benfotiamina sobre parâmetros de estresse oxidativo muscular de camundongos submetidos a treinamento de endurance
title_full_unstemmed Efeito da suplementação com benfotiamina sobre parâmetros de estresse oxidativo muscular de camundongos submetidos a treinamento de endurance
title_sort Efeito da suplementação com benfotiamina sobre parâmetros de estresse oxidativo muscular de camundongos submetidos a treinamento de endurance
author GONÇALVES, Álisson de Carvalho
author_facet GONÇALVES, Álisson de Carvalho
author_role author
dc.contributor.none.fl_str_mv PORTARI, Guilherme Vannucchi
26157082879
http://lattes.cnpq.br/6076945534196087
dc.contributor.author.fl_str_mv GONÇALVES, Álisson de Carvalho
dc.subject.por.fl_str_mv Tiamina.
Estresse Oxidativo.
Esforço Físico
Oxidative stress.
Physical effort.
Thiamine.
Metabolismo e Bioenergética
topic Tiamina.
Estresse Oxidativo.
Esforço Físico
Oxidative stress.
Physical effort.
Thiamine.
Metabolismo e Bioenergética
description Os altos níveis de espécies reativas de oxigênio podem desencadear um desequilíbrio no estado redox capaz de gerar danos oxidativos a macromoléculas. O presente estudo objetivou investigar a influência da suplementação oral com benfotiamina sobre o estresse oxidativo e a atividade antioxidante no músculo de camundongos treinados. Vinte e cinco ratos Balb / c machos foram alocados nos grupos Pad-Sed: dieta padrão e sedentário (n=6); Ben-Sed: suplementação com benfotiamina e sedentário (n=6); Sta-Tr: dieta padrão e treinado (n=6); Ben-Tr: Suplementação com benfotiamina e treinado (n=7). Os camundongos treinados foram submetidos a treinamento de endurance em natação durante 6 semanas, com um teste de exaustão ao final do protocolo de treinamento. A concentração de malondialdeído livre (MDA), proteínas ligadas a malondialdeído (PrMDA) e tióis não proteicos bem como a atividade das enzimas catalase (CAT) e superóxido dismutase (SOD) foram analisadas no músculo gastrocnêmio. Não houve diferença no tempo de resistência à exaustão e na concentração de lactato pós-exaustão. A concentração de MDA foi menor no grupo Ben-Tr em relação aos grupos sedentários. A concentração de PrMDA foi maior no grupo Pad-Sed em relação aos demais grupos. Os nívéis de tióis foram maiores no grupo Ben-Sed em relação aos grupos não suplementados. A atividade da CAT foi mais acentuada em ambos os grupos suplementados. A SOD mostrou-se mais eficiente no grupo Ben-Tr que nos grupos não suplementados. Os resultados mostraram que a suplementação de benfotiamina é uma estratégia antioxidante eficiente, capaz de prevenir os danos oxidativos musculares em indivíduos engajados em programas de treinamento em endurance. Em conclusão, a benfotiamina é eficiente em prevenir danos oxidativos musculares e não interfere no desempenho de endurance.
publishDate 2017
dc.date.none.fl_str_mv 2017-04-01T13:49:14Z
2017-02-03
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.uri.fl_str_mv GONÇALVES, Álisson de Carvalho. Efeito da suplementação com benfotiamina sobre parâmetros de estresse oxidativo muscular de camundongos submetidos a treinamento de endurance. 2017. 54f. Dissertação (Mestrado em Educação Física) - Programa de Pós-Graduação em Educação Física, Universidade Federal do Triângulo Mineiro, Uberaba, 2017.
http://bdtd.uftm.edu.br/handle/tede/367
identifier_str_mv GONÇALVES, Álisson de Carvalho. Efeito da suplementação com benfotiamina sobre parâmetros de estresse oxidativo muscular de camundongos submetidos a treinamento de endurance. 2017. 54f. Dissertação (Mestrado em Educação Física) - Programa de Pós-Graduação em Educação Física, Universidade Federal do Triângulo Mineiro, Uberaba, 2017.
url http://bdtd.uftm.edu.br/handle/tede/367
dc.language.iso.fl_str_mv por
language por
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dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
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application/pdf
dc.publisher.none.fl_str_mv Universidade Federal do Triângulo Mineiro
Instituto de Ciências da Saúde - ICS::Curso de Graduação em Educação Física
Brasil
UFTM
Programa de Pós-Graduação em Educação Física
publisher.none.fl_str_mv Universidade Federal do Triângulo Mineiro
Instituto de Ciências da Saúde - ICS::Curso de Graduação em Educação Física
Brasil
UFTM
Programa de Pós-Graduação em Educação Física
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reponame_str Biblioteca Digital de Teses e Dissertações da UFTM
collection Biblioteca Digital de Teses e Dissertações da UFTM
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da UFTM - Universidade Federal do Triangulo Mineiro (UFTM)
repository.mail.fl_str_mv bdtd@uftm.edu.br||bdtd@uftm.edu.br
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